##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	CHILD25_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	103848
Sequences flagged as poor quality	0
Sequence length	37-301
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.31809953008243	34.0	34.0	34.0	33.0	34.0
2	33.772802557584164	34.0	34.0	34.0	34.0	34.0
3	33.65598759725753	34.0	34.0	34.0	34.0	34.0
4	33.7141880440644	34.0	34.0	34.0	34.0	34.0
5	33.90646907017949	34.0	34.0	34.0	34.0	34.0
6	36.54867691241044	38.0	36.0	38.0	34.0	38.0
7	35.814363300207994	38.0	35.0	38.0	31.0	38.0
8	32.43091826515677	35.0	29.0	37.0	23.0	38.0
9	30.91119713427317	32.0	28.0	37.0	23.0	37.0
10-14	29.63184461905862	31.8	24.0	35.6	18.4	37.6
15-19	35.73761073877205	37.4	35.4	38.0	30.6	38.0
20-24	37.21402819505431	38.0	37.4	38.0	35.6	38.0
25-29	37.180922116940145	38.0	38.0	38.0	36.6	38.0
30-34	37.185211077728994	38.0	38.0	38.0	36.4	38.0
35-39	37.10181392821916	38.0	38.0	38.0	36.0	38.0
40-44	37.05247527612738	38.0	38.0	38.0	35.6	38.0
45-49	37.18401301915318	38.0	37.8	38.0	36.6	38.0
50-54	37.07114312401899	38.0	37.8	38.0	36.0	38.0
55-59	36.718164222365594	38.0	37.2	38.0	34.8	38.0
60-64	36.92644948818936	38.0	37.6	38.0	35.2	38.0
65-69	36.285899448226715	38.0	37.0	38.0	32.6	38.0
70-74	36.9094783672133	38.0	37.4	38.0	35.0	38.0
75-79	36.369903800783845	38.0	37.4	38.0	33.4	38.0
80-84	36.250707290533185	38.0	37.0	38.0	32.8	38.0
85-89	35.79844771635194	38.0	37.0	38.0	31.4	38.0
90-94	36.12612400935992	38.0	37.0	38.0	32.2	38.0
95-99	35.750303812339304	38.0	37.0	38.0	30.6	38.0
100-104	35.71652142093657	38.0	37.0	38.0	30.6	38.0
105-109	36.13884464645103	38.0	37.0	38.0	32.6	38.0
110-114	35.44039018941327	38.0	36.4	38.0	29.2	38.0
115-119	35.271021791674286	38.0	36.4	38.0	28.0	38.0
120-124	35.02743266536346	38.0	35.8	38.0	26.8	38.0
125-129	34.71257715677872	38.0	35.2	38.0	25.8	38.0
130-134	34.67105645805849	38.0	35.4	38.0	24.8	38.0
135-139	34.29601432877213	38.0	34.4	38.0	23.0	38.0
140-144	34.08283917686597	37.8	34.2	38.0	23.2	38.0
145-149	33.762015272468155	37.8	33.8	38.0	22.4	38.0
150-154	33.198332161737945	37.4	32.4	38.0	20.0	38.0
155-159	32.19749053896599	36.2	29.8	37.8	19.0	37.8
160-164	32.05266208942001	35.6	30.2	37.6	19.4	37.6
165-169	30.743618977919436	34.6	25.8	37.6	15.0	37.8
170-174	30.961999865186282	35.6	26.4	37.6	16.2	37.8
175-179	29.52981405336697	33.6	23.6	37.2	12.8	37.6
180-184	28.70764490067118	32.0	22.4	37.2	13.0	37.2
185-189	28.863273854805623	32.6	22.8	37.0	12.2	37.6
190-194	28.857580864155928	32.2	22.8	37.0	12.6	37.4
195-199	26.38001868132926	29.2	19.0	35.2	8.4	37.2
200-204	26.512313307076756	29.2	19.8	35.0	11.2	37.0
205-209	26.222234633643723	28.2	19.2	34.8	9.6	37.2
210-214	25.739526418673627	27.8	18.6	35.0	9.2	37.2
215-219	23.20201642801429	23.6	15.4	32.2	8.0	37.0
220-224	25.98739876934336	27.6	17.6	35.4	11.0	37.4
225-229	23.56976706115728	24.2	14.4	33.6	8.8	36.8
230-234	21.733600392885688	21.6	12.6	29.8	7.8	35.4
235-239	19.53568230184791	19.2	11.4	26.2	7.4	32.0
240-244	20.494641154775778	20.4	11.6	28.2	7.8	34.0
245-249	19.404458482190144	18.8	10.4	26.6	7.4	33.2
250-254	19.224139358864484	18.6	10.6	26.6	7.4	32.0
255-259	18.412354714146776	18.0	10.6	25.4	7.2	31.8
260-264	17.93604052115131	17.2	8.8	24.8	7.2	30.4
265-269	16.1030920488796	13.8	8.0	21.6	7.0	28.4
270-274	16.120161391277556	14.0	8.6	21.6	7.2	28.6
275-279	17.25959151443951	16.0	9.0	23.0	7.2	30.2
280-284	14.964079848238274	13.0	8.2	20.4	7.2	26.6
285-289	14.98773387772396	12.8	8.0	20.0	7.0	27.0
290-294	14.050191146590658	12.0	7.8	19.0	7.0	25.0
295-299	13.728325217440963	12.0	7.6	18.6	7.0	24.4
300-301	14.202410541712858	12.5	9.5	19.0	7.0	24.5
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-1.693756037687681
1101	2	-0.6546274576176927
1101	3	0.16805923224163166
1101	4	0.08126900951145899
1101	5	0.06142645715003425
1101	6	0.5110856695452171
1101	7	1.1264728501882146
1101	8	0.7323499768992932
1101	9	1.6823634073293405
1101	10-14	3.0943527001950777
1101	15-19	1.8019893214971532
1101	20-24	0.46177274730678164
1101	25-29	0.5736476648779671
1101	30-34	0.28534092209946493
1101	35-39	0.3765560414202014
1101	40-44	0.13089922972728374
1101	45-49	0.17431407600253834
1101	50-54	0.22516813372195799
1101	55-59	0.1889564092848275
1101	60-64	0.16419960273715617
1101	65-69	0.5495291263898423
1101	70-74	0.36984383662829856
1101	75-79	0.4077480656550705
1101	80-84	0.21235924518881433
1101	85-89	0.4583747225401922
1101	90-94	0.49470558538747156
1101	95-99	0.5508527758613582
1101	100-104	0.14287445745080873
1101	105-109	0.21167810203861137
1101	110-114	0.3780131698735403
1101	115-119	0.3995852502019659
1101	120-124	0.3217814768633218
1101	125-129	0.2257897065995209
1101	130-134	-0.2244917315621251
1101	135-139	0.10950846686463223
1101	140-144	0.4542272328259216
1101	145-149	0.4517335347819582
1101	150-154	0.18266082707347664
1101	155-159	0.3248052930949967
1101	160-164	-0.016702570104875747
1101	165-169	0.22851323391906675
1101	170-174	-0.5977180996463254
1101	175-179	-0.13311136431118697
1101	180-184	0.10798970097860305
1101	185-189	-0.7089327633374545
1101	190-194	-0.02114703827140829
1101	195-199	-0.8768102767133428
1101	200-204	-0.5533235535359182
1101	205-209	0.23982096410525955
1101	210-214	-0.3344132465068981
1101	215-219	-1.1637264984902203
1101	220-224	-0.8599370432652655
1101	225-229	-2.6926497249705044
1101	230-234	-0.6602165471144197
1101	235-239	-0.4526095787052036
1101	240-244	-1.0722317056826078
1101	245-249	-0.7832302260486337
1101	250-254	-0.9462103409783076
1101	255-259	-1.290344730108874
1101	260-264	-1.140831986124315
1101	265-269	-0.965122339132872
1101	270-274	-0.6260914104193311
1101	275-279	-0.8085093949358431
1101	280-284	-1.1989250220513394
1101	285-289	-0.9685332395517001
1101	290-294	-0.6722640228838888
1101	295-299	-0.7825596433694955
1101	300-301	-0.9027853828246784
1102	1	0.515469573387378
1102	2	0.160535856247769
1102	3	0.31017474165110315
1102	4	0.1739577397731935
1102	5	0.011567058491152693
1102	6	0.6390328899648594
1102	7	1.1528085629288611
1102	8	2.4574202775698524
1102	9	2.3564386831242743
1102	10-14	4.244374331170636
1102	15-19	1.69874467516361
1102	20-24	0.1854586655416952
1102	25-29	0.4952353784838621
1102	30-34	0.25531712802635553
1102	35-39	0.45359476086644435
1102	40-44	0.19051419836236505
1102	45-49	0.32168591247236833
1102	50-54	0.23983393514959772
1102	55-59	0.5271891585818196
1102	60-64	0.46055478335580347
1102	65-69	0.730958933874156
1102	70-74	0.42373741222856154
1102	75-79	0.5245445181750839
1102	80-84	0.6508299352169402
1102	85-89	0.9291188280993552
1102	90-94	0.711750794126381
1102	95-99	0.7096998448641685
1102	100-104	0.762158472159868
1102	105-109	0.6917343425750602
1102	110-114	0.7899967303321205
1102	115-119	0.5958430914306021
1102	120-124	0.5755993440491309
1102	125-129	0.9652986834543853
1102	130-134	0.953001238370824
1102	135-139	0.9462919407992985
1102	140-144	1.06035961439634
1102	145-149	1.3199576316887232
1102	150-154	1.0420270394896534
1102	155-159	1.3387356413537006
1102	160-164	1.1045390478920964
1102	165-169	1.4290756392835533
1102	170-174	1.5792232804099164
1102	175-179	1.1909639114837454
1102	180-184	1.7474662313701188
1102	185-189	2.5200311129333635
1102	190-194	1.4686647722412438
1102	195-199	2.226380292181318
1102	200-204	1.0320214605242164
1102	205-209	1.5023344834649777
1102	210-214	1.0687016139733103
1102	215-219	3.382617866640647
1102	220-224	1.7510947542688022
1102	225-229	1.3445663684753093
1102	230-234	1.1008368813952067
1102	235-239	1.609222564924476
1102	240-244	0.8359448030779362
1102	245-249	0.8709337367460925
1102	250-254	1.0934716836810026
1102	255-259	0.8700165071829282
1102	260-264	0.9025273043796851
1102	265-269	0.310012854464361
1102	270-274	1.5598700864441746
1102	275-279	0.7656524047613225
1102	280-284	0.9627340737738805
1102	285-289	1.076286141802397
1102	290-294	0.830829206620761
1102	295-299	0.36022737563375173
1102	300-301	0.8460964518121123
1103	1	0.41655263400760845
1103	2	0.17549611805234377
1103	3	0.16333821367852863
1103	4	0.21092984511837187
1103	5	0.0912772034186915
1103	6	0.42884900341351084
1103	7	1.6009151547942153
1103	8	3.7194975722558397
1103	9	3.5934263720754984
1103	10-14	7.467500402543553
1103	15-19	1.720335761340948
1103	20-24	0.4585068188843664
1103	25-29	0.350323425858015
1103	30-34	0.40668848535843694
1103	35-39	0.3265961633909953
1103	40-44	0.2953217408276885
1103	45-49	0.32405462059141854
1103	50-54	0.15479419695417818
1103	55-59	0.37545937831066567
1103	60-64	0.3329873842596527
1103	65-69	0.30464601506474764
1103	70-74	0.2948531984214853
1103	75-79	0.4681396560899955
1103	80-84	0.8228417787575282
1103	85-89	0.7331861492774792
1103	90-94	0.6657401972742747
1103	95-99	0.5413813159565848
1103	100-104	0.5914166925120554
1103	105-109	0.5445861424815561
1103	110-114	0.9407291598162999
1103	115-119	0.5188678693642146
1103	120-124	0.6682828410103312
1103	125-129	0.6509461822856153
1103	130-134	0.7405031863215754
1103	135-139	0.7494164538116124
1103	140-144	0.9624789848186523
1103	145-149	0.8319881755565817
1103	150-154	0.9393077002345507
1103	155-159	1.5145420929666145
1103	160-164	2.2802909568838317
1103	165-169	2.0099233874524813
1103	170-174	1.983103598315477
1103	175-179	2.2311774068865837
1103	180-184	2.4926898561168898
1103	185-189	2.296593369439517
1103	190-194	2.5560342595406027
1103	195-199	2.796974027571686
1103	200-204	1.6990543419329711
1103	205-209	2.5339925791483218
1103	210-214	2.9088917339670743
1103	215-219	3.847788507126854
1103	220-224	3.890552444778386
1103	225-229	1.653581484573177
1103	230-234	2.2653919694425753
1103	235-239	1.5939506309989646
1103	240-244	1.3551983483483099
1103	245-249	1.020731149867487
1103	250-254	2.011798004391615
1103	255-259	2.488264909863311
1103	260-264	1.9712821742565723
1103	265-269	2.1541923776060194
1103	270-274	2.494598152518666
1103	275-279	3.352492049455101
1103	280-284	2.208152493596229
1103	285-289	2.001928965551814
1103	290-294	1.3249541871412571
1103	295-299	0.6959375091863951
1103	300-301	1.0878815788155443
1104	1	0.4951157796959862
1104	2	0.17806569426993235
1104	3	0.24751489510379088
1104	4	0.21285373295307863
1104	5	0.04348308577163351
1104	6	0.5502647740228284
1104	7	1.207849057387513
1104	8	3.989922408857147
1104	9	2.4085486575488275
1104	10-14	4.74362838061651
1104	15-19	1.7172549053426422
1104	20-24	0.2554075146465564
1104	25-29	0.25151201957507396
1104	30-34	0.25393179128295884
1104	35-39	0.3417448034921904
1104	40-44	0.05305682410831025
1104	45-49	0.2777600812703156
1104	50-54	0.04437357709333156
1104	55-59	0.05430978944285414
1104	60-64	0.21051215760984832
1104	65-69	0.6213574845987964
1104	70-74	0.15931925366078303
1104	75-79	0.24163744230124706
1104	80-84	0.28665687468838286
1104	85-89	0.5247816584488874
1104	90-94	0.3864417574682406
1104	95-99	0.45092959763485396
1104	100-104	0.0390553178546682
1104	105-109	0.24200075348725392
1104	110-114	0.4443871821650234
1104	115-119	0.09327489023709035
1104	120-124	0.06967436536200466
1104	125-129	0.23884940809206512
1104	130-134	0.4548980840656256
1104	135-139	0.2167736117285486
1104	140-144	0.4018238087697412
1104	145-149	0.7128178192420052
1104	150-154	0.26976574366698003
1104	155-159	0.5281964256674314
1104	160-164	1.0992214860847653
1104	165-169	0.9993868072289871
1104	170-174	1.0626418652351468
1104	175-179	1.5114562388494797
1104	180-184	0.9544888230154775
1104	185-189	1.4506150856528883
1104	190-194	1.730301600885742
1104	195-199	1.3097349383876242
1104	200-204	0.64107943153871
1104	205-209	0.9413903231921736
1104	210-214	0.9267259106484964
1104	215-219	3.614565863230581
1104	220-224	2.5802187951895235
1104	225-229	1.4765996165571522
1104	230-234	2.1328914423500223
1104	235-239	2.273246435342209
1104	240-244	1.0142696112620797
1104	245-249	0.37733825507515917
1104	250-254	1.3441430391797269
1104	255-259	2.030353250575935
1104	260-264	0.8734458892049197
1104	265-269	1.4866006635010187
1104	270-274	1.7028155254893598
1104	275-279	2.9191903416752147
1104	280-284	0.7753726074482223
1104	285-289	1.1461177701058904
1104	290-294	0.98445239502656
1104	295-299	1.2435372414872656
1104	300-301	0.3526966945722325
1105	1	0.5312479745093981
1105	2	0.19700149439643155
1105	3	0.2565281778362376
1105	4	0.21092984511837187
1105	5	0.07694028585596868
1105	6	0.9163042005461293
1105	7	1.496972502464466
1105	8	3.8915405830085277
1105	9	2.1704873039751433
1105	10-14	4.9377512986009044
1105	15-19	1.9117336108033172
1105	20-24	0.27642796583777596
1105	25-29	0.5825814903741531
1105	30-34	0.3830325713799425
1105	35-39	0.42050297342682796
1105	40-44	0.3196945006843208
1105	45-49	0.407208742455218
1105	50-54	0.28454330089684277
1105	55-59	0.38406152884829936
1105	60-64	0.34875799357865134
1105	65-69	0.600703362735004
1105	70-74	0.436071836414321
1105	75-79	0.6731575772369425
1105	80-84	0.6228417787575324
1105	85-89	0.6894585507111657
1105	90-94	0.7668154660914723
1105	95-99	0.6015963697200277
1105	100-104	0.5376532516518395
1105	105-109	0.49082270162134023
1105	110-114	0.8991520988844073
1105	115-119	0.8909108801168983
1105	120-124	0.8510785399350596
1105	125-129	0.8946737808519174
1105	130-134	1.0358436881136939
1105	135-139	0.7838250559621542
1105	140-144	0.9804001317720576
1105	145-149	1.1344971361300509
1105	150-154	1.4683399582990617
1105	155-159	1.8865851037192982
1105	160-164	2.081007802761974
1105	165-169	1.7289198032230857
1105	170-174	1.9494118420430802
1105	175-179	2.375263428391964
1105	180-184	2.0489622575505813
1105	185-189	2.5732958784000886
1105	190-194	2.6628442953829
1105	195-199	2.8485869307974916
1105	200-204	1.9176923347645136
1105	205-209	2.2924155182164228
1105	210-214	2.290253741135537
1105	215-219	4.830584206051583
1105	220-224	3.0016635558895004
1105	225-229	2.1388861440713853
1105	230-234	3.4403023637078114
1105	235-239	2.5982517062677815
1105	240-244	1.7896069504988503
1105	245-249	0.2028100029140809
1105	250-254	2.1465650294812235
1105	255-259	2.2158634761715525
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2119	280-284	-1.1394659994746927
2119	285-289	-1.5513259546764395
2119	290-294	-1.5476775688723574
2119	295-299	-1.1203465710725453
2119	300-301	-0.6762119380320328
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	10.0
16	20.0
17	86.0
18	285.0
19	662.0
20	1376.0
21	1850.0
22	2561.0
23	3368.0
24	4039.0
25	4872.0
26	5895.0
27	7082.0
28	8678.0
29	10601.0
30	12324.0
31	13850.0
32	12631.0
33	8923.0
34	3908.0
35	787.0
36	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.03274015869347508	0.04140667128880672	0.12710885139819736	99.79874431861953
2	0.01733302519066328	0.05970264232339573	0.12229412217856868	99.80067021030737
3	0.06259147985517295	0.11073877205145984	99.72748632616901	0.09918342192435096
4	0.06933210076265311	99.72267159694938	0.05007318388413835	0.15792311840382098
5	0.2195516524150682	0.05873969647947	0.11555350127108852	99.60615514983438
6	99.7977813727756	0.11651644711501426	0.023110700254217704	0.06259147985517295
7	99.77852245589708	0.09244280101687082	0.06548031738695016	0.06355442569909868
8	99.24505045836221	0.2224404899468454	0.42851090054695323	0.10399815114397967
9	41.985401741006086	24.09675679839766	21.571912795624375	12.345928664971883
10-14	58.80748786688237	11.2474000462214	9.513134581311148	20.431977505585085
15-19	39.42203990447577	20.648832909637164	19.90235729142593	20.026769894461136
20-24	55.97334565904013	43.815384022802554	0.1831522995146753	0.028118018642631536
25-29	19.20788074878669	18.927663508204297	60.19143363377244	1.6730221092365765
30-34	8.088745088976196	52.864571296510285	5.599144904090593	33.447538710422926
35-39	63.58298121478012	4.387583343283812	19.807101945543277	12.22233349639279
40-44	36.2192456209616	50.57132127071557	0.6754167188267355	12.534016389496086
45-49	21.193486571590896	19.978622396410103	24.27821699230599	34.54967403969301
50-54	34.44779338835017	25.248875749901295	13.059404701146878	27.243926160601657
55-59	29.773031479002764	27.717507486976032	3.202788718017853	39.30667231600335
60-64	39.99191117702004	4.1104702109834665	5.5464288809498585	50.35118973104663
65-69	46.305044921856194	20.879178021512416	26.37957764788583	6.436199408745559
70-74	40.30583454505185	54.303927893920864	5.184357757084943	0.20587980394233826
75-79	34.85261971939488	22.043583348580125	20.356100802141615	22.747696129883384
80-84	34.28043178907431	5.6138357391162	37.878032104923584	22.227700366885898
85-89	33.94224195210261	11.669860467803597	51.81757778269955	2.5703197973942435
90-94	7.013587296696102	59.98112607971342	12.510712875672864	20.49457374791761
95-99	10.036495998921492	7.592708503856635	56.68050112184271	25.690294375379164
100-104	19.28336880218013	28.54218224888538	33.37833543578534	18.79611351314915
105-109	57.33165137172956	39.169932689437346	0.9696958024786465	2.5287201363544445
110-114	21.219101177694107	63.535586006336246	13.664140514410622	1.5811723015590244
115-119	41.493543385942786	20.147909905919285	22.853428601692872	15.50511810644506
120-124	27.886987587508543	17.502287018402072	27.493909308887112	27.116816085202267
125-129	18.45214594547748	38.2139108496153	24.49314857434495	18.840794630562268
130-134	20.22860554469556	42.415091432588326	26.661145723997805	10.695157298718307
135-139	25.05647731759223	18.446945987847506	21.634520015022098	34.86205667953817
140-144	34.120966421755085	20.115554613999443	19.510048436642368	26.25343052760311
145-149	8.361531868999586	34.23536548961453	31.825859196702844	25.577243444683045
150-154	26.17832002850347	27.56670871570676	19.932207959786993	26.322763296002773
155-159	35.685961077354186	38.640499966296574	2.916983639392568	22.756555316956675
160-164	36.59422034338979	10.407041127813033	5.570117576819744	47.42862095197743
165-169	20.152532090479262	33.279536240815816	28.15199283561393	18.41593883309099
170-174	22.92526505339586	27.531079376390267	19.45978217955261	30.08387339066126
175-179	54.73244292083545	16.572457557753236	13.127389332383219	15.56771018902809
180-184	30.453070382389473	27.55573102737681	1.1853977486109373	40.805800841622776
185-189	28.773098885860932	18.45214594547748	41.79687424769131	10.977880920970273
190-194	36.6554642888095	27.38105096921433	8.089400753030901	27.874083988945277
195-199	19.42838984274943	27.575760493803386	34.43643051797356	18.55941914547363
200-204	58.922838406501874	4.292083545985922	23.543867420339538	13.241210627172666
205-209	24.30229087022254	41.157086868181075	27.950350034184908	6.5902722274114796
210-214	57.69333731354781	22.202470942829354	7.63642666615309	12.467765077469739
215-219	31.09805771952969	19.601143990678594	28.093830346567543	21.206967943224168
220-224	54.985507525494235	10.116036091557772	20.72857184126648	14.169884541681514
225-229	33.667607152830605	28.95798626826004	20.745905033366395	16.62850154554296
230-234	40.61552091057036	33.5599487707878	16.997698537271177	8.82683178137067
235-239	47.52645719183029	25.777730699972075	15.135535932670177	11.56027617552746
240-244	38.416324015137654	34.12424046915174	12.474698354309705	14.984737161400908
245-249	28.869779579573795	18.835787263955627	26.294067233526246	26.00036592294433
250-254	46.454880738008804	14.342253507564012	16.743478386472407	22.45938736795478
255-259	19.220198946527102	30.319027030150124	16.87752173871176	33.583252284611014
260-264	35.67845002744422	31.13811665238283	16.690323264032664	16.493110056140285
265-269	30.60752838310206	23.10302656793167	25.128506360318546	21.16093868864772
270-274	26.204319816653342	13.616185349600855	35.987943801939394	24.19155103180641
275-279	15.527266074128285	44.162469787283214	11.000606661723497	29.309657476865002
280-284	29.195258409005557	19.164154958737374	31.753830153976526	19.88675647828055
285-289	30.985969743950236	15.541902991901546	30.257590493707088	23.21453677044113
290-294	42.9254576444192	20.120561980606084	20.79058615077951	16.16339422419521
295-299	40.365621444218256	25.756244630043888	17.249653613641815	16.628480312096045
300-301	72.71824231747895	22.905167351732235	3.372969269915835	1.0036210608729692
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.25
40	0.5
41	0.75
42	2.0
43	4.583333333333334
44	11.166666666666666
45	89.49999999999993
46	247.666666666668
47	314.41666666666634
48	546.4166666666664
49	1111.5000000000005
50	2408.4999999999986
51	3319.9166666666783
52	3996.7500000000095
53	3763.66666666667
54	3525.7500000000027
55	3698.2500000000023
56	3647.916666666668
57	3086.1666666666674
58	2157.083333333333
59	1166.25
60	634.8333333333334
61	413.6666666666667
62	280.0
63	188.25
64	117.5
65	71.5
66	33.25
67	16.5
68	7.75
69	3.5
70	3.0
71	0.25
72	0.25
73	0.25
74	0.25
75	0.0
76	0.0
77	0.25
78	0.25
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-39	1.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	1444.0
300-302	102403.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.196094291657038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.39181051786431	4.633695400970649
2	14.184397163120568	2.041445189122564
3	5.941389000401445	1.2826438641090825
4	3.331995182657567	0.9590940605500347
5	1.9938445068914759	0.7173946537246745
6	1.3247691690084302	0.5719898312918881
7	1.0169945135822294	0.5122871889684923
8	0.749364378429011	0.43139973807873044
9	0.615549310852402	0.39865957938525537
>10	4.1081225746019	6.112780217240582
>50	0.923323966278603	4.655843155380941
>100	0.8430349257326376	10.827363069100993
>500	0.17395958784959187	8.247631153223942
>1k	0.3880636959721665	53.1690547723596
>5k	0.013381506757660913	5.4387181264925655
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	5648	5.4387181264925655	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	4126	3.9731145520375932	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3656	3.520530005392497	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	3294	3.17194360989138	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3155	3.038094137585702	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2826	2.7212849549341342	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2714	2.613435020414452	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2558	2.4632154687620367	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	2461	2.36980972190124	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	2212	2.1300362067637315	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	2073	1.9961867344580542	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1870	1.8007087281411296	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1725	1.6610815807718973	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1714	1.6504891764887144	No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1663	1.6013789384485018	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1617	1.5570834296279177	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1613	1.5532316462522149	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1599	1.5397504044372545	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1529	1.4723441953624528	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1444	1.390493798628765	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1359	1.3086434018950774	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1189	1.144942608427702	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1182	1.1382019875202218	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1167	1.1237577998613357	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1105	1.06405515753794	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1099	1.0582774824743857	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1094	1.053462753254757	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	1072	1.0322779446883907	No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1062	1.0226484862491332	No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1037	0.99857484015099	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	869	0.836799938371466	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	770	0.7414682998228179	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	707	0.6808027116554964	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	702	0.6759879824358679	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	685	0.6596179030891303	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	684	0.6586549572452045	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	668	0.6432478237423928	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	632	0.6085817733610662	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	616	0.5931746398582544	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	585	0.5633233186965565	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	576	0.5546568061012249	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	570	0.5488791310376704	No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	501	0.48243586780679454	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	418	0.40251136276095834	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	364	0.3505122871889685	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	354	0.3408828287497111	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	347	0.334142207842231	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	323	0.3110315075880133	No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	314	0.3023649949926816	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	308	0.2965873199291272	No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	293	0.2821431322702411	No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	286	0.27540251136276095	No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	277	0.2667359987674293	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	275	0.26481010707957786	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	252	0.24266235266928587	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	251	0.24169940682536015	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	250	0.2407364609814344	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	231	0.2224404899468454	No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	229	0.22051459825899392	No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	217	0.20895924813188504	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	205	0.1974038980047762	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	204	0.1964409521608505	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	193	0.18584854787766736	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	192	0.18488560203374163	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC	185	0.17814498112626145	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	185	0.17814498112626145	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	173	0.1665896309991526	No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	164	0.15792311840382098	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	162	0.1559972267159695	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	161	0.15503428087204374	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	153	0.14733071412063786	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCCTGAA	151	0.14540482243278638	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	147	0.14155303905708344	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	147	0.14155303905708344	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	146	0.14059009321315769	No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	140	0.13481241814960326	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	139	0.13384947230567754	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	138	0.13288652646175178	No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	135	0.12999768892997457	No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	135	0.12999768892997457	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	134	0.12903474308604884	No Hit
CCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	134	0.12903474308604884	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT	132	0.12710885139819736	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	131	0.12614590555427163	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	131	0.12614590555427163	No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	130	0.1251829597103459	No Hit
CCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	127	0.12229412217856868	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	123	0.11844233880286574	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	123	0.11844233880286574	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC	122	0.11747939295894	No Hit
CCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	116	0.11170171789538558	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	115	0.11073877205145984	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	113	0.10881288036360835	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	112	0.10784993451968261	No Hit
CCTACGGGGTGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	111	0.10688698867575687	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	109	0.10496109698790541	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	108	0.10399815114397967	No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC	106	0.10207225945612819	No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	105	0.10110931361220245	No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	104	0.1001463677682767	No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	104	0.1001463677682767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
