FastQCFastQC Report
Thu 10 Oct 2024
CHILD23_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD23_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90364
Sequences flagged as poor quality0
Sequence length158-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT59886.626532690009296No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT44024.871408968173166No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT35753.9562215041388162No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA31203.4527024036120584No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT30553.380771103536807No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT27933.0908326324642554No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT26572.940330219999115No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA25782.85290602452304No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA19892.201097782302687No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT18832.083794431410739No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA17001.8812801558142622No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16451.8204152095967419No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16361.8104554911247843No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA16221.7949625957239608No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA15281.69093886946129No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA14941.6533132663450048No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT13541.4983843123367713No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA13311.47293169846399No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA12881.425346376875747No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT11321.2527112566951442No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA10421.1531140719755655No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA9911.0966756673011377No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9581.0601566995706255No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA9301.0291709087689787No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA8940.9893320348811473No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA8850.9793723164091895No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7950.879775131689611No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA7860.869815413217653No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7680.8498959762737374No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA7600.8410428931875525No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA7150.7912443008277632No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA6760.7480855207826125No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA5770.6385286175910762No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA5750.63631534681953No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5240.579876942145102No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4880.5400380682572705No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA4760.5267584436279935No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA4330.4791731220397504No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA3430.37957593732017175No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3160.34969678190429815No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA3110.3441636049754327No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA3040.33641715727502103No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA2990.33088398034615557No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2880.3187109911026515No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2460.27223230490018147No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2410.266699127971316No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC2400.2655924925855429No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2330.25784604488513124No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA2330.25784604488513124No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2290.2534195033420389No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA2130.23571333716966933No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA2080.23018016024080384No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1850.20472754636802265No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1740.19255455712451863No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC1720.19034128635297243No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1620.17927493249524148No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1520.16820857863751051No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1510.16710194325173744No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT1490.16488867248019123No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1490.16488867248019123No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA1460.16156876632287193No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC1460.16156876632287193No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA1430.15824886016555265No Hit
CCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1420.15714222477977957No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1420.15714222477977957No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGAC1350.14939577707936788No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC1340.14828914169359478No Hit
CCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1280.14164932937895622No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1270.14054269399318314No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA1260.13943605860741004No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1250.13832942322163694No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1220.13500951706431763No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1200.13279624629277145No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1190.13168961090699838No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1180.13058297552122528No Hit
CCTACGGGGAGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1150.127263069363906No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC1130.1250497985923598No Hit
CCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1130.1250497985923598No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1100.1217298924350405No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1100.1217298924350405No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1090.12062325704926742No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1080.11951662166349432No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC1080.11951662166349432No Hit
CCTACGGGGTGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1040.11509008012040192No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGAC1040.11509008012040192No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1040.11509008012040192No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGAC1020.11287680934885574No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT990.10955690319153645No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA980.10845026780576335No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC950.10513036164844407No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT940.10402372626267098No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC920.10181045549112477No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC920.10181045549112477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)