##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	CHILD22_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20530
Sequences flagged as poor quality	0
Sequence length	296-301
%GC	55
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.27652216268875	34.0	34.0	34.0	33.0	34.0
2	33.7690209449586	34.0	34.0	34.0	34.0	34.0
3	33.643789576229906	34.0	34.0	34.0	34.0	34.0
4	33.71729176814418	34.0	34.0	34.0	34.0	34.0
5	33.89220652703361	34.0	34.0	34.0	34.0	34.0
6	36.53906478324404	38.0	36.0	38.0	34.0	38.0
7	35.75893813930833	38.0	35.0	38.0	31.0	38.0
8	32.28684851436922	35.0	29.0	37.0	23.0	38.0
9	30.681393083292743	32.0	28.0	37.0	20.0	37.0
10-14	29.55909400876766	31.8	23.4	35.6	17.0	37.6
15-19	35.69223575255724	37.4	35.4	38.0	30.2	38.0
20-24	37.10870920603993	38.0	37.4	38.0	35.2	38.0
25-29	37.05047247929859	38.0	38.0	38.0	36.2	38.0
30-34	36.981773015099854	38.0	37.8	38.0	35.4	38.0
35-39	36.97684364344861	38.0	37.8	38.0	35.6	38.0
40-44	36.960701412566976	38.0	38.0	38.0	35.0	38.0
45-49	37.019454456892355	38.0	37.8	38.0	36.0	38.0
50-54	36.781217730151	38.0	37.0	38.0	35.0	38.0
55-59	36.467501217730145	38.0	37.2	38.0	33.2	38.0
60-64	36.75629810034097	38.0	37.6	38.0	34.4	38.0
65-69	36.33205065757428	38.0	37.0	38.0	33.4	38.0
70-74	36.69789576229907	38.0	37.2	38.0	34.6	38.0
75-79	35.94640038967365	38.0	37.0	38.0	31.6	38.0
80-84	35.767179736970284	38.0	37.0	38.0	30.8	38.0
85-89	35.32720896249391	38.0	36.6	38.0	28.6	38.0
90-94	35.6023185582075	38.0	36.6	38.0	29.6	38.0
95-99	35.12162688748174	38.0	36.4	38.0	27.4	38.0
100-104	35.27055041402826	38.0	37.0	38.0	27.8	38.0
105-109	35.854856307842184	38.0	36.6	38.0	31.0	38.0
110-114	34.63246955674622	37.6	35.0	38.0	25.8	38.0
115-119	34.72796882610814	38.0	35.4	38.0	25.6	38.0
120-124	34.72284461763273	38.0	35.2	38.0	25.6	38.0
125-129	34.099883097905504	37.6	34.6	38.0	23.8	38.0
130-134	34.212917681441795	37.8	34.4	38.0	23.8	38.0
135-139	33.270112031173895	37.4	32.6	38.0	21.0	38.0
140-144	32.834028251339504	36.8	32.0	38.0	20.0	38.0
145-149	32.96132489040429	37.0	32.0	38.0	20.0	38.0
150-154	31.939698002922558	36.8	28.6	38.0	16.2	38.0
155-159	31.133239162201658	36.0	27.0	37.8	16.6	37.8
160-164	30.667082318558208	34.8	26.0	37.6	15.4	37.6
165-169	28.736697515830492	32.8	22.8	36.8	11.6	37.8
170-174	28.807773989283977	32.0	22.6	37.2	11.4	37.8
175-179	28.249264490988793	31.2	22.0	36.8	11.6	37.6
180-184	26.404588407208962	29.0	18.8	35.4	8.4	37.2
185-189	27.117457379444716	30.0	20.6	36.0	9.8	37.6
190-194	27.51960058451047	30.2	20.6	36.2	11.2	37.4
195-199	25.18261081344374	26.8	17.4	34.4	8.2	37.2
200-204	25.549469069654165	27.8	18.2	34.2	10.0	37.0
205-209	24.764305893813933	25.8	17.6	33.2	9.6	37.2
210-214	24.04543594739406	24.8	16.2	32.8	8.2	36.2
215-219	21.767871407696056	21.6	13.6	30.4	7.6	35.6
220-224	23.456746225036532	23.8	14.6	32.0	10.0	36.8
225-229	22.29087189478812	22.8	13.2	31.0	8.6	35.8
230-234	20.56225036531904	20.2	11.2	28.0	7.8	33.8
235-239	18.417126156843644	18.0	9.6	25.6	7.4	31.6
240-244	18.829069654164634	18.4	10.4	25.6	7.8	32.2
245-249	19.285552849488553	18.8	10.0	26.4	7.4	32.6
250-254	18.228543594739406	17.2	9.6	24.6	7.0	31.0
255-259	17.559493424257187	16.8	8.0	24.4	7.2	30.6
260-264	17.191933755479788	16.2	8.4	23.8	7.0	29.8
265-269	15.311115440818313	13.0	8.0	20.4	7.0	27.0
270-274	16.23529469069654	14.0	8.4	22.0	7.2	29.4
275-279	16.67360935216756	15.2	8.6	22.2	7.4	28.8
280-284	15.009235265465174	13.0	8.0	20.4	7.2	26.6
285-289	13.783955187530442	12.0	8.0	19.0	7.0	24.8
290-294	13.659064783244034	11.6	8.0	18.6	7.0	24.4
295-299	13.577258589012184	11.4	7.6	18.0	7.0	23.2
300-301	13.552813129160707	12.0	7.5	18.5	7.0	23.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-1.5747644306974813
1101	2	-0.9104402046995617
1101	3	0.13471136962655805
1101	4	0.18345871176305906
1101	5	0.11029199724201533
1101	6	1.0363583400381486
1101	7	1.3406108438944742
1101	8	1.452341249920586
1101	9	0.45903318492634426
1101	10-14	3.5654790111269925
1101	15-19	2.1260292285018636
1101	20-24	0.39109870049237827
1101	25-29	0.83736116307999
1101	30-34	0.2975551152545819
1101	35-39	0.42645586621998177
1101	40-44	-0.06034228756907822
1101	45-49	0.42013788626760373
1101	50-54	0.38243440308037435
1101	55-59	-0.2821778095267291
1101	60-64	0.9389410850767845
1101	65-69	1.1054860184084845
1101	70-74	0.4931162506085087
1101	75-79	0.6062086113774754
1101	80-84	0.242686181438188
1101	85-89	1.0746131725619676
1101	90-94	0.3341503570829829
1101	95-99	-0.002362699142928193
1101	100-104	-0.3532165025553482
1101	105-109	0.6291368349461735
1101	110-114	0.08080562169213579
1101	115-119	0.5792658196435241
1101	120-124	0.8716352616854905
1101	125-129	0.46121296491376995
1101	130-134	-0.13812022395252654
1101	135-139	-0.9637394576561604
1101	140-144	0.5738692789298199
1101	145-149	0.24277925806453027
1101	150-154	0.6937270832930125
1101	155-159	0.8538147214563914
1101	160-164	0.14032079123403918
1101	165-169	0.19127727917242865
1101	170-174	-0.8142444752872109
1101	175-179	-0.3372881706778763
1101	180-184	-0.38205410773147364
1101	185-189	0.33284521580813475
1101	190-194	1.1796443082282657
1101	195-199	0.7619319850752113
1101	200-204	-0.2633077908436299
1101	205-209	-0.3288269622983435
1101	210-214	-0.7848907219665762
1101	215-219	-1.6235723240163225
1101	220-224	0.4445642699801553
1101	225-229	-3.0859302629889065
1101	230-234	-0.8188265317912027
1101	235-239	-1.067897573171674
1101	240-244	-0.9143034257860947
1101	245-249	-0.10853811995740514
1101	250-254	-1.6346181876033405
1101	255-259	-0.5275474375488258
1101	260-264	0.5383367874445355
1101	265-269	-0.7629124174183524
1101	270-274	-0.6836873095630089
1101	275-279	0.030678110602696762
1101	280-284	-0.6424680278190991
1101	285-289	0.16331081911129885
1101	290-294	-0.09740572489607047
1101	295-299	-0.003515141022397472
1101	300-301	0.7622593341042911
1102	1	0.7410250429867276
1102	2	0.24745453214254098
1102	3	0.310149966117784
1102	4	0.2536341503595523
1102	5	0.11029199724201533
1102	6	0.8100425505644608
1102	7	1.7055231245962261
1102	8	2.8705868639556726
1102	9	2.2590331849263414
1102	10-14	4.801970239197171
1102	15-19	2.078239754817652
1102	20-24	0.3008530864572876
1102	25-29	0.5238173034308744
1102	30-34	0.4087831854300248
1102	35-39	0.5839997258691056
1102	40-44	0.21993841418531446
1102	45-49	0.5310150792500679
1102	50-54	0.24292563115054833
1102	55-59	0.27438359398204426
1102	60-64	0.5791516113925681
1102	65-69	0.7149597026190051
1102	70-74	0.3671513383278082
1102	75-79	0.2867700148862582
1102	80-84	0.9502651288066133
1102	85-89	0.58738510238652
1102	90-94	0.639343339539117
1102	95-99	3.0396752374173275E-4
1102	100-104	0.5505027956902708
1102	105-109	0.8955227998584618
1102	110-114	0.2699284287096759
1102	115-119	0.2836166968365106
1102	120-124	1.0465124546679547
1102	125-129	0.9850726140365822
1102	130-134	-0.042962329215683326
1102	135-139	0.06468159497541137
1102	140-144	0.9521850684035016
1102	145-149	-0.2222733735144189
1102	150-154	0.21106041662634567
1102	155-159	0.2922708618072676
1102	160-164	0.1959699140410578
1102	165-169	-0.4218455278451181
1102	170-174	0.2678607878706849
1102	175-179	0.5076241100238796
1102	180-184	1.2178757168299335
1102	185-189	2.1584592508958593
1102	190-194	1.7472232555966904
1102	195-199	1.114563564022582
1102	200-204	0.37521852494584707
1102	205-209	0.6563660201577974
1102	210-214	1.0714601552264043
1102	215-219	2.8633048689661322
1102	220-224	2.3209151471731353
1102	225-229	1.5708416668356548
1102	230-234	1.9618050471561652
1102	235-239	0.9164533040213136
1102	240-244	0.7938369250910995
1102	245-249	-0.5146433831153026
1102	250-254	1.5812414615194648
1102	255-259	0.20192624666170644
1102	260-264	1.0438806470936584
1102	265-269	1.172105126441295
1102	270-274	1.3075407606124294
1102	275-279	0.869976356216732
1102	280-284	1.62181267393529
1102	285-289	0.8989599419183207
1102	290-294	1.1334714680863858
1102	295-299	-0.26084847435572556
1102	300-301	1.5452883351784035
1103	1	0.5152185913738236
1103	2	0.24745453214254098
1103	3	0.310149966117784
1103	4	0.2536341503595523
1103	5	0.07803393272588721
1103	6	0.6713328731451114
1103	7	1.5958457052413877
1103	8	4.202199767181483
1103	9	4.378388023636024
1103	10-14	7.710357335971363
1103	15-19	1.9392720128821637
1103	20-24	0.3960788929088963
1103	25-29	0.6359463356889279
1103	30-34	0.22104124994615404
1103	35-39	0.6052900484497528
1103	40-44	0.12290615612079847
1103	45-49	0.20972475666941648
1103	50-54	0.024086921473120526
1103	55-59	0.4810932714013987
1103	60-64	0.0360548371990248
1103	65-69	0.7014113155222432
1103	70-74	0.6361835963923284
1103	75-79	-0.014391275436331341
1103	80-84	0.5387812578388704
1103	85-89	0.17048187658007663
1103	90-94	0.49521430728105287
1103	95-99	0.07075558042696173
1103	100-104	-0.03323913979359361
1103	105-109	0.2093292514713596
1103	110-114	1.000896170645163
1103	115-119	0.7002618581268365
1103	120-124	0.10638342240989829
1103	125-129	0.5244274527462593
1103	130-134	0.5508441223972227
1103	135-139	0.39822998207219484
1103	140-144	0.9743786167905952
1103	145-149	0.9926943684210627
1103	150-154	1.736737835981188
1103	155-159	2.2330450553556496
1103	160-164	1.5967441075894442
1103	165-169	1.5615093108645581
1103	170-174	1.7762478846448815
1103	175-179	2.817946690669043
1103	180-184	2.5866499103783163
1103	185-189	2.0236205412184347
1103	190-194	2.5563845459192684
1103	195-199	2.3694022737000004
1103	200-204	2.1439927184942356
1103	205-209	2.0580434395126375
1103	210-214	2.523202090710278
1103	215-219	3.59504680445
1103	220-224	3.572011921366684
1103	225-229	2.406712634577591
1103	230-234	1.695095369736812
1103	235-239	2.4176145943438954
1103	240-244	1.7482885379943234
1103	245-249	1.6977437136588946
1103	250-254	2.4318221066807553
1103	255-259	1.4232165692423493
1103	260-264	1.5462032277388182
1103	265-269	2.911847061925169
1103	270-274	3.4720568896446906
1103	275-279	2.9467505497651203
1103	280-284	3.0117481578062577
1103	285-289	1.8971534903054135
1103	290-294	1.6728263067960611
1103	295-299	0.31799023532168924
1103	300-301	0.8008841218801823
1104	1	0.5928768948385823
1104	2	0.24745453214254098
1104	3	0.310149966117784
1104	4	0.2536341503595523
1104	5	0.11029199724201533
1104	6	1.0285610690829827
1104	7	1.8055231245962275
1104	8	4.3987350121038205
1104	9	3.1812554071485657
1104	10-14	5.313822091049023
1104	15-19	2.0806101251880236
1104	20-24	0.25581604942025393
1104	25-29	0.6716691552827214
1104	30-34	0.15174614839298783
1104	35-39	0.6402960221654084
1104	40-44	0.1741606364075281
1104	45-49	0.6858298940648808
1104	50-54	0.3085552607801816
1104	55-59	0.42016137175982493
1104	60-64	0.5935219817629402
1104	65-69	0.9245893322486367
1104	70-74	0.636780967957435
1104	75-79	1.0307700148862509
1104	80-84	0.5187095732510585
1104	85-89	0.6389406579420793
1104	90-94	0.8891211173168969
1104	95-99	0.8680076712274385
1104	100-104	0.6270953882828607
1104	105-109	0.6872265035621652
1104	110-114	0.5632617620430125
1104	115-119	-0.5248277476079366
1104	120-124	-0.2071171749616738
1104	125-129	0.012480021443991518
1104	130-134	0.7509635967102426
1104	135-139	0.8861630764569028
1104	140-144	0.4372221054405401
1104	145-149	0.8143192190781647
1104	150-154	1.6085418981078305
1104	155-159	1.0659004914368957
1104	160-164	2.081451395522542
1104	165-169	1.886598916599329
1104	170-174	2.2982311582410553
1104	175-179	1.9128092952090618
1104	180-184	1.9417275686817845
1104	185-189	2.4747555471921494
1104	190-194	2.100112144485575
1104	195-199	1.8330820825410967
1104	200-204	1.3064777842051072
1104	205-209	2.0446623164540902
1104	210-214	1.2147194144856641
1104	215-219	5.705082646743914
1104	220-224	3.4441744064323956
1104	225-229	2.678397222391208
1104	230-234	3.1226939360450565
1104	235-239	2.1020829336509443
1104	240-244	2.1714665547207304
1104	245-249	3.175282542810624
1104	250-254	3.1055377578157604
1104	255-259	3.124889209624669
1104	260-264	2.0373621285751398
1104	265-269	1.3181792005153703
1104	270-274	3.04087409394576
1104	275-279	2.3981245043648833
1104	280-284	1.0772200813426966
1104	285-289	0.904441423399799
1104	290-294	1.3793973940123117
1104	295-299	2.61129967379242
1104	300-301	2.0824715522807598
1105	1	0.6006741657937482
1105	2	0.24745453214254098
1105	3	0.310149966117784
1105	4	0.23609029071042897
1105	5	0.11029199724201533
1105	6	0.878463603196046
1105	7	1.5160494403857
1105	8	4.364621951674977
1105	9	3.0730682726456457
1105	10-14	5.281268484811211
1105	15-19	1.8944502811334445
1105	20-24	0.26127413908886155
1105	25-29	0.49350151395718456
1105	30-34	0.1010638871844094
1105	35-39	0.28961376095682567
1105	40-44	0.41685069488706006
1105	45-49	0.7218922722325232
1105	50-54	-0.03510945656874753
1105	55-59	0.5388748220522217
1105	60-64	0.5845551201645023
1105	65-69	0.4844333868295365
1105	70-74	0.6334671278014881
1105	75-79	0.6448051026055523
1105	80-84	1.4426861814381908
1105	85-89	0.878121944491788
1105	90-94	1.0604661465566707
1105	95-99	1.3064092306816306
1105	100-104	1.092397532532381
1105	105-109	0.71685613319179
1105	110-114	1.231682814674592
1105	115-119	0.6178623108715939
1105	120-124	0.8400563143170814
1105	125-129	1.4155989298260536
1105	130-134	0.8232832848194107
1105	135-139	1.1801201914666422
1105	140-144	0.9177289280526253
1105	145-149	2.1655862756083906
1105	150-154	2.43056918855617
1105	155-159	2.576621739000256
1105	160-164	2.5894435982515773
1105	165-169	2.8088211388215463
1105	170-174	3.5717204369934983
1105	175-179	3.9820100749361593
1105	180-184	3.046016067707118
1105	185-189	3.0696873210712994
1105	190-194	4.200696939807216
1105	195-199	4.379475844724336
1105	200-204	3.1858150161739154
1105	205-209	3.436085318403407
1105	210-214	3.292302260489567
1105	215-219	5.48870837773806
1105	220-224	4.588423919102958
1105	225-229	2.6509118422742475
1105	230-234	4.349594520840377
1105	235-239	3.489997163670438
1105	240-244	2.9839421882489887
1105	245-249	1.0809355642531173
1105	250-254	2.751346724677358
1105	255-259	2.8408736150827565
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2119	280-284	-1.9556418715192567
2119	285-289	-0.7188582398998644
2119	290-294	-1.819255804640889
2119	295-299	-1.8568484743557292
2119	300-301	-0.8040794927608523
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	10.0
17	43.0
18	86.0
19	212.0
20	362.0
21	542.0
22	679.0
23	916.0
24	963.0
25	1164.0
26	1387.0
27	1650.0
28	1873.0
29	2144.0
30	2272.0
31	2423.0
32	1978.0
33	1293.0
34	474.0
35	54.0
36	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.034096444227959086	0.02922552362396493	0.12664393570384802	99.81003409644423
2	0.01948368241597662	0.034096444227959086	0.10228933268387726	99.84413054067218
3	0.07306380905991232	0.07306380905991232	99.77106673161228	0.08280565026790064
4	0.08767657087189479	99.77106673161228	0.03896736483195324	0.10228933268387726
5	0.18996590355577203	0.043838285435947394	0.06819288845591817	99.69800292255236
6	99.83925962006819	0.08767657087189479	0.01948368241597662	0.0535801266439357
7	99.76619581100829	0.043838285435947394	0.07306380905991232	0.11690209449585973
8	99.12323429128105	0.2727715538236727	0.5065757428153921	0.0974184120798831
9	46.283487579152464	21.50998538723819	21.071602532878714	11.134924500730637
10-14	58.63419386264004	11.191427179736971	9.61714564052606	20.55723331709693
15-19	38.260107160253284	21.64052605942523	19.686312713102776	20.413054067218706
20-24	60.96346809547004	38.63321967851924	0.1919142717973697	0.21139795421334634
25-29	17.954213346322454	17.666829030686802	60.23964929371651	4.1393083292742325
30-34	7.577204091573307	54.30979055041403	0.9098879688261081	37.20311738918656
35-39	74.09352167559669	1.469069654164637	19.95908426692645	4.478324403312226
40-44	25.896736483195326	56.45981490501705	0.270823185582075	17.372625426205555
45-49	20.410131514856307	19.886994641987336	21.151485630784215	38.55138821237214
50-54	39.627861665854844	20.322454943984415	14.568923526546518	25.48075986361422
55-59	29.446663419386265	30.344861178762788	0.9868485143692158	39.22162688748173
60-64	39.795421334632245	0.9556746225036532	0.8222113979542133	58.426692644909885
65-69	42.87286897223575	26.15294690696542	25.780808572820263	5.193375547978568
70-74	40.45786653677545	49.22747199220653	9.294690696541647	1.019970774476376
75-79	32.113979542133464	25.621042377009257	22.32537749634681	19.939600584510472
80-84	38.71894788114953	1.1962981003409645	35.54213346322455	24.542620555284948
85-89	28.339016074037993	12.947881149537263	54.5796395518753	4.13346322454944
90-94	9.936678032148077	59.47101802240623	9.44958597174866	21.142717973697028
95-99	9.575255723331711	7.955187530443254	60.680954700438384	21.788602045786654
100-104	13.718460789089137	32.17535314174379	22.35752557233317	31.748660496833903
105-109	53.84413054067218	37.67072576716999	2.0087676570871893	6.476376035070628
110-114	28.132489040428645	57.167072576717004	11.09985387238188	3.6005845104724794
115-119	39.27423283000487	25.259620068192888	19.69020944958597	15.775937652216268
120-124	25.72235752557233	22.003896736483195	23.403799318071115	28.869946419873354
125-129	29.35216755966878	26.46760837798344	28.452021432050657	15.728202630297124
130-134	27.306380905991233	60.42182172430589	8.665367754505601	3.606429615197272
135-139	20.8348757915246	17.111544081831468	7.018996590355578	55.03458353628836
140-144	38.380905991232346	17.505114466634193	18.753044325377495	25.360935216755966
145-149	6.515343399902582	38.51534339990258	21.445689235265466	33.52362396492937
150-154	31.44666341938626	10.653677545056016	23.56746225036532	34.3321967851924
155-159	36.73453482708232	37.97369702873843	1.6210423770092546	23.670725767169994
160-164	37.02776424744277	7.483682415976619	1.3073550901120312	54.18119824646859
165-169	28.668290306868	30.909887968826112	30.56697515830492	9.854846566000974
170-174	18.2484169508037	40.15197272284462	19.68046760837798	21.919142717973696
175-179	75.00340964442279	7.880175353141744	10.93424257184608	6.1821724305893815
180-184	31.095957135898683	30.770579639551876	3.42523136872869	34.70823185582075
185-189	29.68144179249878	15.129079396005846	44.39064783244034	10.79883097905504
190-194	35.91134924500731	20.235752557233315	10.088650754992694	33.76424744276668
195-199	11.886994641987336	25.575255723331708	45.45640526059425	17.0813443740867
200-204	54.54359473940574	5.984413054067219	28.70238675109596	10.769605455431076
205-209	29.81782756941062	43.61324890404286	21.20116902094496	5.3677545056015585
210-214	65.01607403799318	21.60740379931807	8.737457379444715	4.6390647832440335
215-219	42.691670725767175	17.050170482221137	18.252313687286897	22.00584510472479
220-224	54.281539210910864	14.001948368241598	16.679006332196785	15.037506088650755
225-229	38.14125669751583	10.315635655138822	33.52946906965417	18.013638577691182
230-234	29.85874330248417	36.587433024841694	17.95031660983926	15.603507062834876
235-239	48.037018996590355	25.994154895275205	18.255236239649296	7.713589868485143
240-244	33.04140282513395	43.66293229420361	17.64150024354603	5.6541646371164145
245-249	30.41013151485631	23.35119337554798	17.48952752070141	28.749147588894303
250-254	50.9985387238188	7.945445689235266	19.64052605942523	21.415489527520702
255-259	11.743789576229908	34.79201169020945	12.326351680467608	41.13784705309303
260-264	42.37603507062835	18.286410131514856	18.292255236239647	21.045299561617146
265-269	31.560642961519726	19.987335606429614	18.869946419873358	29.5820750121773
270-274	29.39503166098393	14.805650267900633	40.23283000487092	15.56648806624452
275-279	16.57379444715051	45.219678519240134	15.752557233317097	22.453969800292256
280-284	36.14905017048222	25.749634680954703	18.998538723818804	19.102776424744278
285-289	32.73063809059912	17.369702873843156	28.431563565513883	21.46809547004384
290-294	45.59571358986849	18.48319532391622	22.058451047247928	13.862640038967363
295-299	56.612601372426695	22.29121179039301	10.66457423580786	10.431612601372427
300-301	71.54589135124534	22.658854677387723	4.449401843498725	1.3458521278682094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.25
40	0.25
41	0.25
42	0.25
43	0.5
44	0.5
45	1.25
46	4.25
47	19.75
48	48.666666666666664
49	54.66666666666667
50	74.0
51	268.4166666666667
52	469.4166666666666
53	566.1666666666666
54	866.7500000000001
55	1204.4166666666665
56	1144.9166666666665
57	734.4166666666667
58	424.5
59	246.58333333333334
60	166.58333333333331
61	111.33333333333333
62	80.08333333333333
63	63.25
64	57.583333333333336
65	45.0
66	34.25
67	28.5
68	44.0
69	42.58333333333333
70	24.333333333333332
71	9.75
72	5.0
73	3.75
74	1.75
75	0.25
76	0.25
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
296	1.0
297	21.0
298	13.0
299	24.0
300	150.0
301	20321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.065270336093521
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.03835284618489	7.847053093034583
2	13.605167541380702	3.2830004870920604
3	5.61162696810658	2.0311738918655626
4	3.310456197012515	1.5976619581100828
5	1.5341138473960436	0.9254749147588894
6	1.2515139281388776	0.9059912323429129
7	0.8074283407347598	0.6819288845591817
8	0.8074283407347598	0.7793472966390648
9	0.4844570044408559	0.5260594252313687
>10	5.248284214775938	12.752070141256697
>50	1.2111425111021397	9.736970287384315
>100	0.7670569236980218	18.855333658061372
>500	0.20185708518368994	17.778860204578663
>1k	0.12111425111021397	22.299074525085242
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1997	9.727228446176326	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1520	7.4037993180711155	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1061	5.1680467608377985	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	953	4.64198733560643	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	834	4.062347783731125	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	800	3.896736483195324	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	551	2.6838772528007793	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	512	2.4939113492450073	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	470	2.289332683877253	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	398	1.9386264003896738	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC	305	1.4856307842182173	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	293	1.4271797369702874	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	268	1.3054067218704335	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	254	1.2372138334145153	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC	239	1.164150024354603	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	227	1.1056989771066732	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC	150	0.7306380905991232	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC	149	0.725767169995129	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	143	0.6965416463711641	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC	142	0.69167072576717	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	135	0.6575742815392108	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC	129	0.6283487579152459	No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	119	0.5796395518753045	No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	118	0.5747686312713103	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	118	0.5747686312713103	No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC	110	0.535801266439357	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	104	0.5065757428153921	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	96	0.4676083779834389	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	90	0.438382854359474	No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC	88	0.4286410131514856	No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	86	0.4188991719434973	No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC	85	0.41402825133950316	No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	84	0.40915733073550903	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	82	0.3994154895275207	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	77	0.37506088650754993	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	76	0.3701899659035558	No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC	71	0.345835362883585	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	71	0.345835362883585	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	71	0.345835362883585	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC	70	0.34096444227959083	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC	67	0.3263516804676084	No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC	65	0.31660983925962005	No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC	60	0.2922552362396493	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	59	0.28738431563565514	No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC	59	0.28738431563565514	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	58	0.28251339503166095	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	57	0.2776424744276669	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	55	0.2679006332196785	No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	55	0.2679006332196785	No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC	54	0.26302971261568436	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	54	0.26302971261568436	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	52	0.25328787140769604	No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC	52	0.25328787140769604	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	52	0.25328787140769604	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	51	0.2484169508037019	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	51	0.2484169508037019	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT	51	0.2484169508037019	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	47	0.2289332683877253	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	46	0.2240623477837311	No Hit
CCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT	46	0.2240623477837311	No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC	45	0.219191427179737	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	43	0.20944958597174865	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	41	0.19970774476376035	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	39	0.18996590355577203	No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	39	0.18996590355577203	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	37	0.18022406234778374	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	36	0.17535314174378958	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC	36	0.17535314174378958	No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	36	0.17535314174378958	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	36	0.17535314174378958	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	34	0.16561130053580128	No Hit
CCTACGGGGGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC	33	0.1607403799318071	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	33	0.1607403799318071	No Hit
CCTACGGGCGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC	33	0.1607403799318071	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	32	0.15586945932781296	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	32	0.15586945932781296	No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC	31	0.1509985387238188	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	31	0.1509985387238188	No Hit
CCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT	30	0.14612761811982464	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	30	0.14612761811982464	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	30	0.14612761811982464	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	29	0.14125669751583048	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	29	0.14125669751583048	No Hit
CCTACGGGGAGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	29	0.14125669751583048	No Hit
CCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT	28	0.13638577691183634	No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC	28	0.13638577691183634	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT	27	0.13151485630784218	No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC	27	0.13151485630784218	No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC	27	0.13151485630784218	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	26	0.12664393570384802	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGAC	26	0.12664393570384802	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	26	0.12664393570384802	No Hit
CCTACGGGGGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC	25	0.12177301509985387	No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC	25	0.12177301509985387	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	25	0.12177301509985387	No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	24	0.11690209449585973	No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	24	0.11690209449585973	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	24	0.11690209449585973	No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	23	0.11203117389186555	No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC	22	0.1071602532878714	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCTTGAT	22	0.1071602532878714	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT	21	0.10228933268387726	No Hit
CCTACGGGTGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC	21	0.10228933268387726	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT	21	0.10228933268387726	No Hit
CCTACGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATG	21	0.10228933268387726	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	21	0.10228933268387726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.004870920603994155	0.0	0.0	0.0
245-249	0.0	0.004870920603994155	0.0	0.0	0.0
250-254	0.0	0.004870920603994155	0.0	0.0	0.0
255-259	0.0	0.004870920603994155	0.0	0.0	0.0
260-264	0.0	0.004870920603994155	0.0	0.0	0.0
265-269	0.0	0.004870920603994155	0.0	0.0	0.0
270-274	0.0	0.004870920603994155	0.0	0.0	0.0
275-279	0.0	0.004870920603994155	0.0	0.0	0.0
280-284	0.0	0.004870920603994155	0.0	0.0	0.0
285-289	0.0	0.004870920603994155	0.0	0.0	0.0
290	0.0	0.004870920603994155	0.0	0.0	0.0
>>END_MODULE
