FastQCFastQC Report
Thu 10 Oct 2024
CHILD15_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD15_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16216
Sequences flagged as poor quality0
Sequence length73-301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3282.0226936359151457No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA3171.9548593981253082No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2461.517020226936359No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2461.517020226936359No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2351.449185989146522No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2181.3443512580167736No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2101.295017266896892No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2011.2395165268870252No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1881.1593487913172176No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1821.1223482979773063No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1791.1038480513073508No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1771.0915145535273805No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1651.0175135668475581No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1610.9928465712876172No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1590.9805130735076467No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1500.92501233349778No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1410.8695115934879132No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1360.8386778490379871No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1330.8201776023680316No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1310.8078441045880612No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1280.7893438579181057No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1260.7770103601381352No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA1250.7708436112481499No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1200.740009866798224No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1190.7338431179082389No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1060.6536753823384311No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT980.6043413912185496No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA910.5611741489886531No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA880.5426739023186976No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT870.5365071534287124No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT830.5118401578687716No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA800.493339911198816No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT760.46867291563887514No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA760.46867291563887514No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT730.45017266896891955No Hit
CCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT730.45017266896891955No Hit
CCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT720.4440059200789344No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT670.41317217562900843No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT640.39467192895905284No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT600.370004933399112No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA590.3638381845091268No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA580.35767143561914155No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT580.35767143561914155No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT560.3453379378391712No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT520.32067094227923043No Hit
CCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT490.3021706956092748No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC480.2960039467192896No Hit
CCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT470.2898371978293044No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT470.2898371978293044No Hit
CCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT460.2836704489393192No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC430.26517020226936355No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC420.2590034533793784No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC410.2528367044893932No Hit
CCTACGGGCGGCTGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT400.246669955599408No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC390.2405032067094228No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCCTGAA370.2281697089294524No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT370.2281697089294524No Hit
CCTACGGGAGGCTGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT370.2281697089294524No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT360.2220029600394672No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC350.21583621114948198No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT350.21583621114948198No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC350.21583621114948198No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA340.20966946225949679No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT330.2035027133695116No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC330.2035027133695116No Hit
CCTACGGGAGGCAGCAGTGAGGAACACTGGTCAATGGACGAGAGTCTGAA320.19733596447952642No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC320.19733596447952642No Hit
CCTACGGGTGGCTGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT320.19733596447952642No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA300.185002466699556No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC300.185002466699556No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC290.17883571780957078No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA290.17883571780957078No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC290.17883571780957078No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC290.17883571780957078No Hit
CCTACGGGTGGCAGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT290.17883571780957078No Hit
CCTACGGGTGGCAGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT280.1726689689195856No Hit
CCTACGGGCGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT280.1726689689195856No Hit
CCTACGGGTGGCTGCAGTGAGGAACACTGGTCAATGGACGAGAGTCTGAA270.16650222002960038No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT270.16650222002960038No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC260.16033547113961522No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT260.16033547113961522No Hit
CCTACGGGAGGCAGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT250.15416872224963No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC250.15416872224963No Hit
CCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGCAACCCTGAT240.1480019733596448No Hit
CCTACGGGGGGCAGCAGTGAGGAACACTGGTCAATGGACGAGAGTCTGAA240.1480019733596448No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT240.1480019733596448No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC240.1480019733596448No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT240.1480019733596448No Hit
CCTACGGGAGGCAGCAGTGGGGAACACTGCACAATGGGCGCAAGCCTGAT240.1480019733596448No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC230.1418352244696596No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT230.1418352244696596No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCCTGAA220.1356684755796744No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT220.1356684755796744No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA220.1356684755796744No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC210.1295017266896892No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT210.1295017266896892No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT210.1295017266896892No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC210.1295017266896892No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTTAGCCTGAA210.1295017266896892No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA200.123334977799704No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT200.123334977799704No Hit
CCTACGGGTGGCTGCAGTGGGGAACATTGCACAATGGGCGCAAGCCTGAT200.123334977799704No Hit
CCTACGGGTGGCAGCAGTGGGGAACACTGCACAATGGGCGCAAGCCTGAT200.123334977799704No Hit
CCTACGGGAGGCAGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT200.123334977799704No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT200.123334977799704No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC200.123334977799704No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC200.123334977799704No Hit
CCTACGGGTGGCTGCAGTGGGGAACACTGCACAATGGGCGAAAGCCTGAT190.11716822890971879No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT190.11716822890971879No Hit
CCTACGGGTGGCTGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT190.11716822890971879No Hit
CCTACGGGTGGCTGCAGTGGGGAACACTGCACAATGGGCGCAAGCCTGAT190.11716822890971879No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC190.11716822890971879No Hit
CCTACGGGAGGCAGCAGTGAGGAACATTGGTCAATGGACGAGAGTCTGAA190.11716822890971879No Hit
CCTACGGGAGGCAGCAGTGGGGAACATTGCACAATGGGCGCAAGCCTGAT190.11716822890971879No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT180.1110014800197336No Hit
CCTACGGGGGGCTGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT180.1110014800197336No Hit
CCTACGGGAGGCAGCAGTGGGGAACATTGCACAATGGGCGAAAGCCTGAT180.1110014800197336No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA180.1110014800197336No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC180.1110014800197336No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA180.1110014800197336No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA180.1110014800197336No Hit
CCTACGGGTGGCAGCAGTGAGGAACACTGGTCAATGGACGAGAGTCTGAA180.1110014800197336No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA170.10483473112974839No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGTAAGTCTGAA170.10483473112974839No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA170.10483473112974839No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC170.10483473112974839No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGAAAGCCTGAT170.10483473112974839No Hit
CCTACGGGCGGCAGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT170.10483473112974839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)