FastQCFastQC Report
Thu 10 Oct 2024
CHILD14_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD14_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21549
Sequences flagged as poor quality0
Sequence length81-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2261.0487725648521973No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2231.03485080514177No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG2080.965242006589633No Hit
GACTACACGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1740.8074620632047891No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1580.7332126780825096No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1450.6728850526706576No Hit
GACTACTCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1390.6450415332498027No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1280.5939950809782357No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1130.5243862824260986No Hit
GACTACTAGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG1130.5243862824260986No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG1100.5104645227156712No Hit
GACTACCGGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG1090.5058239361455288No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1090.5058239361455288No Hit
GACTACCAGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG990.45941807044410415No Hit
GACTACACGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG980.45477748387396166No Hit
GACTACAAGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG980.45477748387396166No Hit
GACTACAGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG930.4315745510232494No Hit
GACTACTCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG910.4222933778829644No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG890.4130122047426795No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG870.40373103160239454No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG860.39909044503225205No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG850.39444985846210956No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG840.3898092718919671No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG810.37588751218153976No Hit
GACTACCAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG780.36196575247111235No Hit
GACTACCCGGGTATCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCAG770.35732516590096985No Hit
GACTACACGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG740.3434034061905425No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG730.3387628196204No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG730.3387628196204No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG710.3294816464801151No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG690.32020047333983015No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG690.32020047333983015No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG680.3155598867696877No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG670.3109193001995452No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG660.3062787136294028No Hit
GACTACCCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG630.29235695391897537No Hit
GACTACCGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG610.28307578077869044No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG570.26451343449812054No Hit
GACTACAGGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG570.26451343449812054No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG530.24595108821755068No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG530.24595108821755068No Hit
GACTACTCGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG530.24595108821755068No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG520.24131050164740825No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG520.24131050164740825No Hit
GACTACAAGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC500.2320293285071233No Hit
GACTACTGGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG490.22738874193698083No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA490.22738874193698083No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG480.22274815536683837No Hit
GACTACTCGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC460.21346698222655344No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG460.21346698222655344No Hit
GACTACCGGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG460.21346698222655344No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG460.21346698222655344No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG460.21346698222655344No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG450.20882639565641095No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG450.20882639565641095No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG440.20418580908626852No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA430.19954522251612603No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG430.19954522251612603No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG420.19490463594598356No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG400.18562346280569864No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA400.18562346280569864No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG400.18562346280569864No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG390.18098287623555617No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG390.18098287623555617No Hit
GACTACTAGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC390.18098287623555617No Hit
GACTACACGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC380.1763422896654137No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA380.1763422896654137No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG380.1763422896654137No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA380.1763422896654137No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG370.17170170309527125No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG360.16706111652512878No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG360.16706111652512878No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA360.16706111652512878No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG340.15777994338484386No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA340.15777994338484386No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG340.15777994338484386No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG340.15777994338484386No Hit
GACTACAGGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC340.15777994338484386No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG340.15777994338484386No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG340.15777994338484386No Hit
GACTACCCGGGTATCTAATCCTGTTCTATCCCCACGCTTTCGTGCCTCAG330.1531393568147014No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA320.14849877024455893No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG310.14385818367441644No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA300.13921759710427398No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG300.13921759710427398No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG300.13921759710427398No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA300.13921759710427398No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG300.13921759710427398No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG290.1345770105341315No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG290.1345770105341315No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG290.1345770105341315No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG290.1345770105341315No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG290.1345770105341315No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA290.1345770105341315No Hit
GACTACTGGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC290.1345770105341315No Hit
GACTACCAGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC280.12993642396398905No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG280.12993642396398905No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG280.12993642396398905No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA270.1252958373938466No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG270.1252958373938466No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG260.12065525082370412No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG260.12065525082370412No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA260.12065525082370412No Hit
GACTACAAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGAGCCTCAG250.11601466425356165No Hit
GACTACACGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG250.11601466425356165No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG250.11601466425356165No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA250.11601466425356165No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG240.11137407768341918No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA240.11137407768341918No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG240.11137407768341918No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG240.11137407768341918No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG240.11137407768341918No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG230.10673349111327672No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG230.10673349111327672No Hit
GACTACCGGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC230.10673349111327672No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA230.10673349111327672No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA230.10673349111327672No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA220.10209290454313426No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG220.10209290454313426No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG220.10209290454313426No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG220.10209290454313426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)