FastQCFastQC Report
Thu 10 Oct 2024
CHILD14_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD14_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21549
Sequences flagged as poor quality0
Sequence length81-301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT2481.1508654693953315No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2251.0441319782820548No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2201.0209290454313424No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA2080.965242006589633No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2020.9373984871687782No Hit
CCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1970.9141955543180659No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1940.9002737946076385No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1780.826024409485359No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1760.8167432363450741No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1760.8167432363450741No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1670.7749779572137918No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1610.7471344377929371No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1540.7146503318019397No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1520.7053691586616548No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1510.7007285720915124No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1490.6914473989512274No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1430.6636038795303726No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1380.6404009466796603No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1360.6311197735393754No Hit
CCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1310.607916840688663No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1310.607916840688663No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1300.6032762541185206No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1280.5939950809782357No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1250.5800733212678082No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1220.5661515615573809No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1180.547589215276811No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1150.5336674555663836No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1150.5336674555663836No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA1120.5197456958559562No Hit
CCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1110.5151051092858138No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1080.5011833495753863No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1060.49190217643510137No Hit
CCTACGGGCGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1050.48726158986495893No Hit
CCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1020.4733398301545315No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA1010.4686992435843891No Hit
CCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1000.4640586570142466No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1000.4640586570142466No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT990.45941807044410415No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC960.44549631073367674No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA940.43621513759339187No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT930.4315745510232494No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT920.4269339644531069No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT900.4176527913128219No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC880.40837161817253703No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT880.40837161817253703No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA860.39909044503225205No Hit
CCTACGGGTGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC860.39909044503225205No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT820.3805280987516822No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA800.37124692561139727No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA780.36196575247111235No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA770.35732516590096985No Hit
CCTACGGGGGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC730.3387628196204No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC720.33412223305025757No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT710.3294816464801151No Hit
CCTACGGGAGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC690.32020047333983015No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC690.32020047333983015No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT680.3155598867696877No Hit
CCTACGGGCGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT680.3155598867696877No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA670.3109193001995452No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC620.2877163673488329No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC570.26451343449812054No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC570.26451343449812054No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA570.26451343449812054No Hit
CCTACGGGGGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC550.2552322613578356No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC520.24131050164740825No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA520.24131050164740825No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC520.24131050164740825No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC520.24131050164740825No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT520.24131050164740825No Hit
CCTACGGGTGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC510.23666991507726576No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT510.23666991507726576No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC510.23666991507726576No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC500.2320293285071233No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT480.22274815536683837No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC470.21810756879669593No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC470.21810756879669593No Hit
CCTACGGGCGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC470.21810756879669593No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC460.21346698222655344No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC450.20882639565641095No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT450.20882639565641095No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC450.20882639565641095No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC440.20418580908626852No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA430.19954522251612603No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC420.19490463594598356No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC410.1902640493758411No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC410.1902640493758411No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT400.18562346280569864No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC390.18098287623555617No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT380.1763422896654137No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT380.1763422896654137No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT380.1763422896654137No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC360.16706111652512878No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT360.16706111652512878No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC360.16706111652512878No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC350.16242052995498632No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT350.16242052995498632No Hit
CCTACGGGCGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC350.16242052995498632No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC350.16242052995498632No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC340.15777994338484386No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC340.15777994338484386No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC340.15777994338484386No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC340.15777994338484386No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC320.14849877024455893No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA320.14849877024455893No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC320.14849877024455893No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC320.14849877024455893No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC320.14849877024455893No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA320.14849877024455893No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT310.14385818367441644No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC310.14385818367441644No Hit
CCTACGGGAGGCAGCAGTGGGGAACACTGCACAATGGGCGCAAGCCTGAT310.14385818367441644No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC300.13921759710427398No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC300.13921759710427398No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC300.13921759710427398No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA290.1345770105341315No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT280.12993642396398905No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGGACAATGGGGGCAACCCTGAT280.12993642396398905No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC280.12993642396398905No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA280.12993642396398905No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGGGAAACCCTGAC270.1252958373938466No Hit
CCTACGGGTGGCTGCAGTGGGGAACACTGCACAATGGGCGCAAGCCTGAT270.1252958373938466No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTGAC270.1252958373938466No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT250.11601466425356165No Hit
CCTACGGGAGGCAGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT250.11601466425356165No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC240.11137407768341918No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC240.11137407768341918No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC230.10673349111327672No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC230.10673349111327672No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA230.10673349111327672No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA230.10673349111327672No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA230.10673349111327672No Hit
CCTACGGGAGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC230.10673349111327672No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA220.10209290454313426No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC220.10209290454313426No Hit
CCTACGGGTGGCTGCAGTGAGGAACACTGGTCAATGGGCGGAAGCCTGAA220.10209290454313426No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA220.10209290454313426No Hit
CCTACGGGTGGCTGCAGTGAGGAACATTGGTCAATGGACGAGAGTCTGAA220.10209290454313426No Hit
CCTACGGGTGGCAGCAGTGGGGAACACTGCACAATGGGCGCAAGCCTGAT220.10209290454313426No Hit
CCTACGGGAGGCTGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT220.10209290454313426No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT220.10209290454313426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)