FastQCFastQC Report
Thu 10 Oct 2024
CHILD13_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD13_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8231
Sequences flagged as poor quality0
Sequence length71-301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG810.984084558376868No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA790.9597861742194144No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA760.923338597983234No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA730.8868910217470539No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG650.7896974851172398No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG630.7653991009597861No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG590.7168023326448791No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG580.7046531405661524No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA560.6803547564086988No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA540.6560563722512452No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA540.6560563722512452No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG530.6439071801725186No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG530.6439071801725186No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA520.6317579880937918No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG510.619608796015065No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA500.6074596039363382No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG480.5831612197788847No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA470.5710120277001579No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG450.5467136435427045No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA450.5467136435427045No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG420.5102660673065241No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA420.5102660673065241No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA410.49811687522779735No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG400.4859676831490706No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG400.4859676831490706No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG400.4859676831490706No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA390.47381849107034385No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG390.47381849107034385No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG350.4252217227554368No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG350.4252217227554368No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG330.40092333859798324No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG330.40092333859798324No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG320.38877414651925646No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA320.38877414651925646No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG320.38877414651925646No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG310.3766249544405297No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG310.3766249544405297No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG310.3766249544405297No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA280.3401773782043494No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG270.3280281861256226No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG270.3280281861256226No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA270.3280281861256226No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG260.3158789940468959No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG250.3037298019681691No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG240.29158060988944234No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG230.27943141781071557No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA230.27943141781071557No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG200.2429838415745353No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA190.2308346494958085No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG190.2308346494958085No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG190.2308346494958085No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG180.21868545741708176No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG170.20653626533835498No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG170.20653626533835498No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG160.19438707325962823No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG160.19438707325962823No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG160.19438707325962823No Hit
GACTACACGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG150.18223788118090148No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG150.18223788118090148No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG150.18223788118090148No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG140.1700886891021747No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG130.15793949702344795No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG130.15793949702344795No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG120.14579030494472117No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG120.14579030494472117No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG120.14579030494472117No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG120.14579030494472117No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG110.1336411128659944No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG110.1336411128659944No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG110.1336411128659944No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG110.1336411128659944No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG110.1336411128659944No Hit
GACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG110.1336411128659944No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG100.12149192078726764No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG100.12149192078726764No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG100.12149192078726764No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG100.12149192078726764No Hit
GACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG100.12149192078726764No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG100.12149192078726764No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG100.12149192078726764No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG90.10934272870854088No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG90.10934272870854088No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG90.10934272870854088No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG90.10934272870854088No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG90.10934272870854088No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG90.10934272870854088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)