FastQCFastQC Report
Thu 10 Oct 2024
CHILD12_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD12_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21399
Sequences flagged as poor quality0
Sequence length85-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5892.752465068461143No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4562.1309406981634655No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4462.084209542501986No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3681.7197065283424458No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2981.3925884387120895No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2921.3645497453152018No Hit
CCTACGGGTGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC2741.2804336651245385No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2191.0234123089864011No Hit
CCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT2181.0187391934202534No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2151.0047198467218095No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2070.9673349221926258No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2010.9392962287957382No Hit
CCTACGGGGGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1930.9019113042665545No Hit
CCTACGGGAGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1710.7991027618112996No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1610.7523716061498201No Hit
CCTACGGGTGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1590.7430253750175242No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1410.6589092948268611No Hit
CCTACGGGCGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1300.6075050235992336No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1270.5934856769007898No Hit
CCTACGGGGGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1220.57012009907005No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1220.57012009907005No Hit
CCTACGGGAGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1200.5607738679377541No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1160.5420814056731623No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1140.5327351745408664No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1110.5187158278424225No Hit
CCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1110.5187158278424225No Hit
CCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1080.5046964811439787No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1080.5046964811439787No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1070.5000233655778308No Hit
CCTACGGGCGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1060.49535025001168276No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1050.49067713444553485No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1000.46731155661479507No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC1000.46731155661479507No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC980.45796532548249924No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC980.45796532548249924No Hit
CCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT980.45796532548249924No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA970.4532922099163512No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA860.4018879386887238No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT860.4018879386887238No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC840.39254170755642787No Hit
CCTACGGGCGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT810.378522360857984No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT810.378522360857984No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA780.3645030141595402No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC780.3645030141595402No Hit
CCTACGGGAGGCAGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT760.35515678302724424No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC750.3504836674610963No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC750.3504836674610963No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA730.34113743632880045No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA720.3364643207626525No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC710.3317912051965045No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA710.3317912051965045No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC710.3317912051965045No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT700.32711808963035655No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA660.30842562736576473No Hit
CCTACGGGTGGCAGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT620.289733165101173No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT620.289733165101173No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC610.285060049535025No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC610.285060049535025No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC600.28038693396887704No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA600.28038693396887704No Hit
CCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT600.28038693396887704No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT590.27571381840272907No Hit
CCTACGGGTGGCTGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT590.27571381840272907No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA580.27104070283658116No Hit
CCTACGGGTGGCTGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT570.2663675872704332No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA570.2663675872704332No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC570.2663675872704332No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC550.25702135613813726No Hit
CCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT550.25702135613813726No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT530.24767512500584138No Hit
CCTACGGGCGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT530.24767512500584138No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC530.24767512500584138No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC520.24300200943969344No Hit
CCTACGGGAGGCTGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT510.23832889387354547No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC480.22430954717510163No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA470.2196364316089537No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA460.21496331604280572No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC450.2102902004766578No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT430.2009439693443619No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT420.19627085377821393No Hit
CCTACGGGAGGCAGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT420.19627085377821393No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT400.18692462264591803No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC390.1822515070797701No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT390.1822515070797701No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC380.17757839151362212No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC370.17290527594747418No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT370.17290527594747418No Hit
CCTACGGGTGGCAGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT350.16355904481517827No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC350.16355904481517827No Hit
CCTACGGGGGGCTGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT350.16355904481517827No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT340.15888592924903033No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC340.15888592924903033No Hit
CCTACGGGGGGCAGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT330.15421281368288237No Hit
CCTACGCGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT330.15421281368288237No Hit
CCTACGGGCGGCAGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT330.15421281368288237No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA320.14953969811673443No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC310.1448665825505865No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT300.14019346698443852No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC300.14019346698443852No Hit
CCTACGGGGGGCTGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT290.13552035141829058No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC290.13552035141829058No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC290.13552035141829058No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC260.12150100471984672No Hit
CCTACGCGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT250.11682788915369877No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT250.11682788915369877No Hit
CCTACGGGAGGCTGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT250.11682788915369877No Hit
CCTACGCGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT240.11215477358755081No Hit
CCTACGGGAGGCTGCAGTGGGGAACACTGCACAATGGGCGAAAGCCTGAT240.11215477358755081No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT240.11215477358755081No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC240.11215477358755081No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC230.10748165802140286No Hit
CCTACGGGAGGCAGCAGTGGGGAATACTGCACAATGGGGGAAACCCTGAT230.10748165802140286No Hit
CCTACGGGCGGCTGCAGTGGGGAACACTGCACAATGGGGGAAACCCTGAT230.10748165802140286No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC220.10280854245525492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)