##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	CHILD25_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	103809
Sequences flagged as poor quality	0
Sequence length	37-301
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.31837316610313	34.0	34.0	34.0	33.0	34.0
2	33.772948395611174	34.0	34.0	34.0	34.0	34.0
3	33.65590652062923	34.0	34.0	34.0	34.0	34.0
4	33.7144274581202	34.0	34.0	34.0	34.0	34.0
5	33.906771089211915	34.0	34.0	34.0	34.0	34.0
6	36.550125711643496	38.0	36.0	38.0	34.0	38.0
7	35.819649548690386	38.0	35.0	38.0	31.0	38.0
8	32.435598069531544	35.0	29.0	37.0	23.0	38.0
9	30.91589361230722	32.0	28.0	37.0	23.0	37.0
10-14	29.63469063376008	31.8	24.0	35.6	18.4	37.6
15-19	35.74041749751949	37.4	35.4	38.0	30.6	38.0
20-24	37.21685210338218	38.0	37.4	38.0	35.6	38.0
25-29	37.18323844753345	38.0	38.0	38.0	36.6	38.0
30-34	37.187839204693226	38.0	38.0	38.0	36.4	38.0
35-39	37.10466890788839	38.0	38.0	38.0	36.0	38.0
40-44	37.05644651664612	38.0	38.0	38.0	35.6	38.0
45-49	37.18726880394574	38.0	37.8	38.0	36.6	38.0
50-54	37.07499229346486	38.0	37.8	38.0	36.0	38.0
55-59	36.72271115906288	38.0	37.2	38.0	34.8	38.0
60-64	36.930151041217954	38.0	37.6	38.0	35.2	38.0
65-69	36.29018659627964	38.0	37.0	38.0	32.8	38.0
70-74	36.914148371497106	38.0	37.4	38.0	35.0	38.0
75-79	36.374739661101856	38.0	37.4	38.0	33.4	38.0
80-84	36.2544168930505	38.0	37.0	38.0	32.8	38.0
85-89	35.802963924464024	38.0	37.0	38.0	31.4	38.0
90-94	36.13098212995521	38.0	37.0	38.0	32.2	38.0
95-99	35.7552255895085	38.0	37.0	38.0	30.6	38.0
100-104	35.722215289623534	38.0	37.0	38.0	30.6	38.0
105-109	36.1450283834198	38.0	37.0	38.0	32.6	38.0
110-114	35.44676011639872	38.0	36.4	38.0	29.2	38.0
115-119	35.28165922999428	38.0	36.4	38.0	28.0	38.0
120-124	35.04530365736035	38.0	35.8	38.0	26.8	38.0
125-129	34.73302051031047	38.0	35.2	38.0	25.8	38.0
130-134	34.6975331545387	38.0	35.4	38.0	24.8	38.0
135-139	34.32986489574854	38.0	34.6	38.0	23.2	38.0
140-144	34.131755276367734	37.8	34.2	38.0	23.4	38.0
145-149	33.82573352293251	37.8	33.8	38.0	22.6	38.0
150-154	33.29238079499872	37.4	32.6	38.0	20.6	38.0
155-159	32.371069612411716	36.4	30.2	37.8	20.0	37.8
160-164	32.32192934513728	35.6	30.6	37.6	20.2	37.6
165-169	31.0764652443783	34.8	26.8	37.8	16.6	37.8
170-174	31.30735450218928	35.8	27.2	37.6	17.2	37.8
175-179	29.989622630970814	33.6	24.2	37.2	15.4	37.6
180-184	29.353824201479846	32.4	24.0	37.2	16.0	37.2
185-189	29.56992259093152	33.2	24.0	37.0	15.0	37.6
190-194	29.702379321455112	32.8	24.8	37.0	16.2	37.4
195-199	27.395295449729826	30.4	20.6	35.8	12.0	37.2
200-204	27.610555060201182	30.0	21.8	35.4	13.2	37.2
205-209	27.39870527485839	30.0	21.0	35.6	12.2	37.4
210-214	27.314833918986913	29.6	21.0	35.8	12.6	37.2
215-219	24.929756667923247	26.0	18.2	33.4	9.6	37.0
220-224	27.50578856622106	29.2	20.0	36.4	12.6	37.4
225-229	25.11091271193661	26.2	17.2	34.0	10.0	37.0
230-234	23.690525761353033	25.0	17.2	31.4	9.4	36.6
235-239	21.84014770592907	22.2	15.8	28.2	9.0	33.0
240-244	23.004816703641104	23.0	16.2	30.8	9.6	34.8
245-249	21.93888664660415	21.8	15.2	29.2	8.0	34.6
250-254	22.317843616538738	22.8	15.8	28.8	9.4	34.4
255-259	21.745509077881383	21.6	16.0	28.8	8.0	33.2
260-264	21.431563093808972	21.2	14.8	28.6	7.4	33.6
265-269	19.89273986761661	19.2	12.6	27.0	8.0	32.0
270-274	19.999815193474067	20.2	12.0	26.6	8.6	32.8
275-279	21.298956755895652	21.0	14.4	27.8	8.8	34.0
280-284	19.24059560979374	19.4	12.8	25.2	8.0	30.8
285-289	19.94287528938946	19.2	13.2	26.6	8.0	30.8
290-294	19.123907330206936	19.0	12.0	25.4	8.0	30.8
295-299	18.41329117720842	18.4	10.6	24.4	7.6	30.0
300-301	17.307008316836587	17.5	10.0	23.0	7.0	29.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-1.6634439638589704
1101	2	-0.7694167496090145
1101	3	0.1661468693054502
1101	4	0.1573870111926965
1101	5	0.0447818058725602
1101	6	0.5383434488495027
1101	7	1.1878391647095654
1101	8	1.4259163782552022
1101	9	1.833604181821368
1101	10-14	3.241029078399105
1101	15-19	1.7130305605254108
1101	20-24	0.3184878367020261
1101	25-29	0.46959534860883423
1101	30-34	0.2835113485052432
1101	35-39	0.3236555681023674
1101	40-44	0.16351637047370104
1101	45-49	0.3168350007196281
1101	50-54	0.22156896849889307
1101	55-59	0.4553544981611495
1101	60-64	0.4053789446507423
1101	65-69	0.7647643762934777
1101	70-74	0.5593800593655018
1101	75-79	0.5618453483667665
1101	80-84	0.772765908019629
1101	85-89	0.8652380383614613
1101	90-94	0.8456685463142222
1101	95-99	0.7558025520524154
1101	100-104	0.7971305208432895
1101	105-109	0.5892410299228317
1101	110-114	0.7237739232770792
1101	115-119	0.663648200848094
1101	120-124	0.3192821808625794
1101	125-129	0.5034227087529359
1101	130-134	0.1874301392886366
1101	135-139	0.5523011115834606
1101	140-144	0.83673817009646
1101	145-149	0.9154823608783715
1101	150-154	0.6445132926297745
1101	155-159	0.6353390086820454
1101	160-164	0.8252588396604708
1101	165-169	1.2613225314171999
1101	170-174	0.6502815860277416
1101	175-179	0.6834545410649646
1101	180-184	1.1619312336489145
1101	185-189	0.17301186088980813
1101	190-194	1.4089200299183595
1101	195-199	0.40803866001009936
1101	200-204	0.23815252917466978
1101	205-209	0.9016139262177489
1101	210-214	0.7628383678576895
1101	215-219	-0.3050476994093074
1101	220-224	-0.30321451147496603
1101	225-229	-1.567374424386081
1101	230-234	0.43886865139315034
1101	235-239	-0.2459995561541639
1101	240-244	-0.7711225686283889
1101	245-249	-0.5709886249576037
1101	250-254	-0.16354047868643917
1101	255-259	-0.7413498860709957
1101	260-264	-0.5611327409957134
1101	265-269	-1.3353657533703256
1101	270-274	0.4945359537901908
1101	275-279	-1.3308484648098897
1101	280-284	-1.6288906461029313
1101	285-289	-0.8938629464967427
1101	290-294	-0.13306653235608934
1101	295-299	-0.49085207178741896
1101	300-301	-0.002851890040833638
1102	1	0.63349525309971
1102	2	0.19072817792721963
1102	3	0.18242417841209146
1102	4	0.21914344640791938
1102	5	0.05321788633979452
1102	6	0.42013016743051423
1102	7	1.3286027381467278
1102	8	2.462040107435385
1102	9	2.770495810633829
1102	10-14	4.369138531135423
1102	15-19	1.8714450100170765
1102	20-24	0.2558769779522905
1102	25-29	0.5029503129177328
1102	30-34	0.3323973532640565
1102	35-39	0.4902573418423586
1102	40-44	0.23301669493832833
1102	45-49	0.3482756236917197
1102	50-54	0.2850018043197906
1102	55-59	0.3195660491021002
1102	60-64	0.4461208871123574
1102	65-69	0.7018290529103908
1102	70-74	0.4774094774922659
1102	75-79	0.4769870312566624
1102	80-84	0.7097981381731984
1102	85-89	0.7699211445695582
1102	90-94	0.7795426540364758
1102	95-99	0.8546993722990024
1102	100-104	0.6294904602765641
1102	105-109	0.6386461780950157
1102	110-114	0.8013750480878059
1102	115-119	0.4667197993771879
1102	120-124	0.32608335286816725
1102	125-129	0.6332327494585002
1102	130-134	0.5650610755165886
1102	135-139	0.7299184792903759
1102	140-144	0.6716002282039355
1102	145-149	1.1597299074155885
1102	150-154	0.9897404356836574
1102	155-159	1.487646542792632
1102	160-164	1.4914012474385672
1102	165-169	1.0748525080709541
1102	170-174	1.2724546016755163
1102	175-179	1.1546539942874467
1102	180-184	1.818021806281532
1102	185-189	2.0860625065686307
1102	190-194	1.4905852541128475
1102	195-199	2.430180753730564
1102	200-204	1.298230950654503
1102	205-209	2.0236470390692034
1102	210-214	1.8658154537847622
1102	215-219	3.8142591114752236
1102	220-224	1.4154389743610274
1102	225-229	1.163673361840754
1102	230-234	0.857952911472811
1102	235-239	1.0140022321378837
1102	240-244	0.2114310867199194
1102	245-249	0.9193972011316518
1102	250-254	1.1968381216451789
1102	255-259	0.45050571069582546
1102	260-264	0.8947953557819766
1102	265-269	0.8584548449484259
1102	270-274	0.9099599838773287
1102	275-279	0.10534646794090818
1102	280-284	0.5116036924409286
1102	285-289	1.0900729964071196
1102	290-294	0.715682000450439
1102	295-299	0.938849295176464
1102	300-301	3.4092572919259396
1103	1	0.5805109925418179
1103	2	0.21983056221894515
1103	3	0.31687173957774206
1103	4	0.26289442038329724
1103	5	0.08582658199196658
1103	6	0.8148239495716965
1103	7	1.6602218748149582
1103	8	4.0494251783407975
1103	9	3.135308028180969
1103	10-14	7.644586559809252
1103	15-19	1.9572717203178414
1103	20-24	0.47015156109083733
1103	25-29	0.5723129434304326
1103	30-34	0.3125516309639167
1103	35-39	0.30277876930068004
1103	40-44	0.07884028851789537
1103	45-49	0.3333465290992308
1103	50-54	0.16009141005455518
1103	55-59	0.3831082027564605
1103	60-64	0.4286282893252249
1103	65-69	0.5744096980716833
1103	70-74	0.41965663348416626
1103	75-79	0.2973158453442508
1103	80-84	0.5252882425833647
1103	85-89	0.4632747365907548
1103	90-94	0.640669348753633
1103	95-99	0.4783163272623838
1103	100-104	0.4800358864881815
1103	105-109	0.4464068139061368
1103	110-114	0.5867654174192722
1103	115-119	0.47051440744949247
1103	120-124	0.3147697965475942
1103	125-129	0.5868202372395288
1103	130-134	0.6331718607886643
1103	135-139	0.5173254522457356
1103	140-144	0.6668664386959549
1103	145-149	1.1172892684931952
1103	150-154	1.1628696695228626
1103	155-159	1.4704481968002767
1103	160-164	1.426642348238225
1103	165-169	1.2243871923503917
1103	170-174	1.6781411178003118
1103	175-179	1.9787940849205583
1103	180-184	2.26039840490305
1103	185-189	2.0823345163410814
1103	190-194	2.147140853049361
1103	195-199	2.13059144283687
1103	200-204	1.3412501670996662
1103	205-209	2.3354079784788127
1103	210-214	2.0368131418725355
1103	215-219	4.093756756687156
1103	220-224	3.90254739797739
1103	225-229	2.1585786957680995
1103	230-234	1.7336375214667257
1103	235-239	2.323160117242338
1103	240-244	1.1414809583088257
1103	245-249	1.3910506522517032
1103	250-254	2.3059886844747517
1103	255-259	2.769631687103633
1103	260-264	1.6243402309284676
1103	265-269	2.006820282028567
1103	270-274	2.3645692373763545
1103	275-279	3.1142835535267146
1103	280-284	1.211566994025251
1103	285-289	1.0848355698737429
1103	290-294	0.8640432465223142
1103	295-299	1.1054707621146598
1103	300-301	2.2000009628120196
1104	1	0.606328024905693
1104	2	0.2047529920884017
1104	3	0.3121236275804904
1104	4	0.22525339881179463
1104	5	0.07475647129086127
1104	6	0.7330479705215893
1104	7	1.6710274667418403
1104	8	4.193402747148738
1104	9	3.2011070732681937
1104	10-14	4.93045464429655
1104	15-19	1.8304508392057954
1104	20-24	0.3981733574378268
1104	25-29	0.6011534253836359
1104	30-34	0.4060563724629418
1104	35-39	0.4867304152687453
1104	40-44	0.33839933572127023
1104	45-49	0.48557708366283947
1104	50-54	0.19109036520539746
1104	55-59	0.3672397214909964
1104	60-64	0.5265663654801784
1104	65-69	0.8387909225290855
1104	70-74	0.32853520614085596
1104	75-79	0.5943810095442785
1104	80-84	0.7503778483181307
1104	85-89	0.8185948704372521
1104	90-94	0.7639562590420965
1104	95-99	0.9686653597849713
1104	100-104	0.749895294759682
1104	105-109	0.6656743614203293
1104	110-114	1.2822857919778485
1104	115-119	0.6845629995483264
1104	120-124	0.8569618636295573
1104	125-129	1.2271408339938858
1104	130-134	1.1060097504578863
1104	135-139	1.1428832562108724
1104	140-144	1.366510660944627
1104	145-149	1.4921768646655167
1104	150-154	1.4452849181357834
1104	155-159	1.48817404072539
1104	160-164	2.3116343446538536
1104	165-169	2.123224043734602
1104	170-174	2.753453972482209
1104	175-179	2.3617885275488675
1104	180-184	2.1939276546953757
1104	185-189	2.8259003351775718
1104	190-194	2.933774899162959
1104	195-199	2.1003472370979566
1104	200-204	1.6532440052095616
1104	205-209	2.4614703249924546
1104	210-214	1.4778460473600425
1104	215-219	4.196590764784322
1104	220-224	2.752201202819169
1104	225-229	2.360094404190246
1104	230-234	2.2351396238983945
1104	235-239	2.299057779972017
1104	240-244	0.7451558467589123
1104	245-249	1.583771014345615
1104	250-254	2.330161277245683
1104	255-259	2.5677671405393596
1104	260-264	1.4267605636993395
1104	265-269	2.5992213567931515
1104	270-274	1.8168412409999277
1104	275-279	2.548305637837423
1104	280-284	0.8613707336171359
1104	285-289	0.9518008254941215
1104	290-294	0.8348882919633951
1104	295-299	1.6122132266421723
1104	300-301	-0.36962520541228017
1105	1	0.5273875926442244
1105	2	0.16629753610526876
1105	3	0.1955711763416943
1105	4	0.2165554546894981
1105	5	0.08582658199196658
1105	6	0.9564138113228537
1105	7	1.4870395911488004
1105	8	3.8864707492573345
1105	9	2.3670796615655085
1105	10-14	4.890805197802084
1105	15-19	1.8376147822738034
1105	20-24	0.25484178126492907
1105	25-29	0.482484474408416
1105	30-34	0.3843642269700638
1105	35-39	0.4435596192750779
1105	40-44	0.3662775645035552
1105	45-49	0.4286793503997899
1105	50-54	0.27071609003407815
1105	55-59	0.3542244341952667
1105	60-64	0.34240453100468926
1105	65-69	0.5670671481484817
1105	70-74	0.2871092704529232
1105	75-79	0.38494769378255
1105	80-84	0.6075207054609848
1105	85-89	0.7775194882548604
1105	90-94	0.6357538341606954
1105	95-99	0.5827428505598746
1105	100-104	0.5299770027196118
1105	105-109	0.630861499005988
1105	110-114	0.9288698721043644
1105	115-119	0.9903326358161166
1105	120-124	0.7730135241461653
1105	125-129	0.8445314507572022
1105	130-134	0.762931205386721
1105	135-139	0.8786977000695941
1105	140-144	0.93784698145069
1105	145-149	1.2593239541087655
1105	150-154	1.6589605387629547
1105	155-159	1.7166331842941531
1105	160-164	2.1781474257646565
1105	165-169	1.9794258360547943
1105	170-174	1.9459352893066146
1105	175-179	2.450053830716108
1105	180-184	3.121744425652853
1105	185-189	2.916977514275075
1105	190-194	2.9646190362992293
1105	195-199	4.098953399557853
1105	200-204	2.7657717421404513
1105	205-209	2.093718819851482
1105	210-214	2.188161460970086
1105	215-219	4.9305746672312125
1105	220-224	3.3412009470464206
1105	225-229	2.0737313154093258
1105	230-234	3.1748980831359503
1105	235-239	2.214458288558813
1105	240-244	1.6010493589063977
1105	245-249	0.7506185039259741
1105	250-254	1.4781326842167175
1105	255-259	2.7806172786549403
1105	260-264	1.363295441514751
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2119	275-279	-1.4414723506487128
2119	280-284	0.22224127374884262
2119	285-289	-0.4596092646813901
2119	290-294	-1.4124103690109848
2119	295-299	-0.6972056287186383
2119	300-301	0.9289483312330695
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	20.0
21	69.0
22	178.0
23	407.0
24	926.0
25	1599.0
26	2626.0
27	3671.0
28	5125.0
29	7047.0
30	10313.0
31	15134.0
32	19618.0
33	19782.0
34	12476.0
35	4169.0
36	628.0
37	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.032752458842682235	0.041422227359862826	0.12715660491864866	99.79866870887881
2	0.017339537034361183	0.05972507200724407	0.12234006685354834	99.80059532410485
3	0.06261499484630427	0.11078037549730754	99.72738394551533	0.09922068414106677
4	0.06935814813744473	99.72256740745023	0.05009199587704342	0.15798244853529075
5	0.21963413576857496	0.058761764394224	0.11559691356240788	99.6060071862748
6	99.79770540126579	0.11656022117542794	0.023119382712481578	0.06261499484630427
7	99.77940255661841	0.09247753084992631	0.06550491768536447	0.06261499484630427
8	99.24669344661831	0.22059744338159504	0.4286718877939293	0.10403722220616711
9	41.9828723906405	24.099066554922985	21.573273993584372	12.344787060852141
10-14	58.80819582117157	11.248350335712704	9.512084694005337	20.431369149110385
15-19	39.421630109142754	20.64907666965292	19.902320608039766	20.026972613164563
20-24	55.97202554691789	43.8166247627855	0.1832211079964165	0.02812858230018592
25-29	19.207775819052298	18.927645965186063	60.19131289194578	1.6732653238158541
30-34	8.089664672619907	52.865551156450785	5.598358523827414	33.44642564710189
35-39	63.58105205156412	4.387690422566146	19.80702947539992	12.224228050469806
40-44	36.222063810110974	50.57066892725031	0.6754778051787916	12.531789457459928
45-49	21.19412762022195	19.97919235511714	24.276356350184958	34.550323674475955
50-54	34.448982737361284	25.24911374845869	13.058723797780516	27.243179716399506
55-59	29.773042540073984	27.71828760789149	3.203028668310728	39.3056411837238
60-64	39.99271728219761	4.111074933000923	5.5462327348531995	50.34997504994827
65-69	46.30381380831736	20.87913146512278	26.380301906422492	6.43675282013737
70-74	40.30556706195151	54.30481566753688	5.1842361305114295	0.20538114000019264
75-79	34.855067577331006	22.044756133979405	20.351806718236727	22.74836957045286
80-84	34.28202608712406	5.6139336839874385	37.87680866231239	22.227231566576116
85-89	33.94627629444382	11.670128278737481	51.812625957081146	2.570969469737547
90-94	7.013534993497424	59.98420114638024	12.508260681084726	20.494003179037616
95-99	10.037262820414014	7.593407525008863	56.6799482096891	25.68938144488802
100-104	19.282057605715487	28.54563776104938	33.377519936957015	18.794784696278114
105-109	57.333030186839714	39.17083011400749	0.9678208712507297	2.5283188279020656
110-114	21.21803604356983	63.53811203835193	13.66345333084128	1.5803985872369615
115-119	41.49832915139392	20.148275769033607	22.851889966814287	15.50150511275819
120-124	27.885390199986894	17.50357601391079	27.494245605647592	27.11678818045472
125-129	18.45278725824801	38.216771755268894	24.492393127783885	18.838047858699213
130-134	20.2238651948067	42.424055381849676	26.664763510320444	10.687315913023184
135-139	25.05625045830133	18.440501873728817	21.638190877198397	34.865056790771455
140-144	34.128447808060194	20.114362077056324	19.513777714264748	26.243412400618737
145-149	8.360636408094466	34.23834569461921	31.85423154167174	25.546786355614575
150-154	26.16257798752974	27.580533073681394	19.946030596857465	26.310858341931397
155-159	35.718769983109716	38.625492471287494	2.9038070192829113	22.75193052631988
160-164	36.59367757920612	10.406513914562515	5.590836436965332	47.40897206926603
165-169	20.07178381604808	33.30427423930949	28.25250781944878	18.371434125193645
170-174	22.946412745928274	27.4896114382496	19.515134805128305	30.048841010693817
175-179	54.73805541833563	16.610224637293005	13.158829259389314	15.492890684982056
180-184	30.401421030447786	27.441944483471488	1.1625153041767857	40.99411918190394
185-189	28.61183254161826	18.595673849884335	42.06064882114538	10.731844787352022
190-194	36.73013971728376	27.467408872006498	7.803821509734267	27.99862990097548
195-199	19.206514329314693	27.494939345106435	34.70744098682249	18.591105338756382
200-204	58.76073702578576	4.109751099581293	23.61321779103519	13.516294083597755
205-209	23.9591214717299	41.316634980377366	28.68034285493824	6.043900692954498
210-214	58.15394278535945	21.908560234375344	7.009078969908068	12.92841801035714
215-219	30.00147678605914	19.52765565893058	29.527996455713456	20.942871099296823
220-224	55.086665781777064	9.668715643780065	21.08356256550853	14.161056008934333
225-229	32.116784916292325	30.198202232515612	21.31758537529679	16.36742747589527
230-234	41.50132411340129	33.36814229191552	16.92660317060347	8.203930424079724
235-239	47.25768886855622	26.12421759653975	15.388134500613445	11.229959034290586
240-244	38.124650909338605	33.844016075199235	12.595327293781079	15.436005721681084
245-249	26.991763584036505	19.32950561338108	27.601068227715725	26.07766257486669
250-254	46.83017335277937	15.230675503535362	16.065747287708998	21.87340385597627
255-259	18.428485206192956	29.93074104085178	17.57783713307257	34.06293661988269
260-264	33.99935151493317	32.05245523651691	17.27023813812732	16.677955110422594
265-269	29.24991324178276	22.09194718490242	27.294548278882225	21.363591294432602
270-274	24.575735071650577	13.516425080910599	38.97828020280368	22.929559644635145
275-279	12.256926011128808	46.83831770188167	11.419807703651271	29.484948583338248
280-284	27.14954843936308	19.239392746504187	35.11675538135908	18.49430343277366
285-289	27.306788468540034	14.957137655975906	33.802005759110315	23.934068116373748
290-294	39.20332095397902	22.387015197181643	22.247846867122078	16.16181698171726
295-299	35.94769416910978	28.86313928056215	18.538787286518684	16.65037926380939
300-301	72.12650573499064	24.758842443729904	2.562748956183712	0.5519028650957432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.5833333333333333
43	0.25
44	2.583333333333333
45	91.41666666666649
46	272.1666666666684
47	239.6666666666682
48	431.4999999999959
49	1551.9166666666013
50	5237.3333333330365
51	4527.750000000261
52	5381.083333332971
53	8068.416666664292
54	7768.24999999798
55	5076.666666666576
56	3611.083333333588
57	4045.5000000003774
58	3189.333333333486
59	1707.1666666666215
60	809.0000000000077
61	394.3333333333315
62	179.33333333333366
63	100.33333333333323
64	62.500000000000036
65	35.08333333333332
66	13.000000000000002
67	4.166666666666666
68	3.0
69	1.0
70	0.0
71	0.0
72	0.0
73	0.5
74	0.5
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-39	1.0
40-49	0.0
50-59	0.0
60-69	1.0
70-79	1.0
80-89	1.0
90-99	2.0
100-109	5.0
110-119	38.0
120-129	55.0
130-139	93.0
140-149	220.0
150-159	844.0
160-169	818.0
170-179	1686.0
180-189	2048.0
190-199	2936.0
200-209	2699.0
210-219	4844.0
220-229	3396.0
230-239	9778.0
240-249	7014.0
250-259	9832.0
260-269	12638.0
270-279	7318.0
280-289	10613.0
290-299	6046.0
300-302	20882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.181458255064589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.30583501006036	4.618096696818195
2	14.232059020791416	2.0441387548285794
3	5.969148222669349	1.2860156633817879
4	3.3266264252179747	0.9556011521159051
5	1.9986586183769282	0.7176641716999489
6	1.3279678068410463	0.572204722133919
7	1.0060362173038229	0.5057364968355345
8	0.7511737089201878	0.4315618106329894
9	0.6170355466130114	0.3988093517903072
>10	4.118041582830315	6.115076727451377
>50	0.9389671361502347	4.753923070254024
>100	0.8316566063044937	10.731246809043531
>500	0.1743796109993293	8.248803090290822
>1k	0.38900067069081157	53.18132339199877
>5k	0.013413816230717638	5.439798090724311
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	5647	5.439798090724311	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	4124	3.9726805960947504	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3655	3.5208893255883402	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	3294	3.1731352772880963	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3155	3.039235519078307	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2825	2.7213440067816856	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2714	2.614416861736458	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2558	2.464140874105328	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	2460	2.3697367280293613	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	2212	2.1308364400003854	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	2072	1.9959733741775763	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1870	1.801385236347523	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1724	1.6607423248465931	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1714	1.6511092487163925	No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1663	1.6019805604523694	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1617	1.5576684102534462	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1613	1.553815179801366	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1599	1.5403288732190852	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1529	1.4728973403076804	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1444	1.3910161932009748	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1359	1.3091350460942695	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1189	1.145372751880858	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1181	1.1376662909766977	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1167	1.1241799843944167	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1105	1.0644549123871727	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1099	1.0586750667090523	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1094	1.053858528643952	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	1072	1.0326657611575105	No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1062	1.0230326850273097	No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1037	0.9989499947018081	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	869	0.8371143157144372	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	770	0.7417468620254506	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	706	0.6800951747921664	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	702	0.6762419443400861	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	685	0.659865714918745	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	684	0.6589024073057249	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	668	0.6434894854974038	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	632	0.6088104114286815	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	616	0.5933974896203605	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	584	0.5625716460037183	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	576	0.5548651850995578	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	570	0.5490853394214374	No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	501	0.48261711412305286	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	418	0.40266258224238743	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	364	0.3506439711393039	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	354	0.34101089500910325	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	347	0.3342677417179628	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	322	0.31018505139246116	No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	313	0.30151528287528057	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	308	0.29669874481018027	No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	293	0.2822491306148792	No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	286	0.2755059773237388	No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	277	0.2668362088065582	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	275	0.264909593580518	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	252	0.24275351848105656	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	251	0.2417902108680365	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	250	0.2408269032550164	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	231	0.2225240586076352	No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	229	0.22059744338159504	No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	216	0.20807444441233422	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	205	0.19747806066911347	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	204	0.19651475305609342	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	193	0.1859183693128727	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	192	0.18495506169985262	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC	185	0.17821190840871215	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	185	0.17821190840871215	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	173	0.16665221705247135	No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	164	0.15798244853529075	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	162	0.15605583330925063	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	161	0.15509252569623058	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	153	0.14738606479207006	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGTTAGCCTGAA	151	0.14545944956602994	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	147	0.14160621911394966	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC	147	0.14160621911394966	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	146	0.14064291150092958	No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	140	0.1348630658228092	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	139	0.13389975820978914	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	138	0.13293645059676906	No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	135	0.13004652775770886	No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	135	0.13004652775770886	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	134	0.12908322014468882	No Hit
CCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	134	0.12908322014468882	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT	132	0.12715660491864866	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	131	0.12619329730562862	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	131	0.12619329730562862	No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	130	0.12522998969260854	No Hit
CCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	127	0.12234006685354834	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	123	0.11848683640146808	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	123	0.11848683640146808	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC	122	0.117523528788448	No Hit
CCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	116	0.11174368311032762	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT	115	0.11078037549730754	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	113	0.10885376027126742	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	112	0.10789045265824734	No Hit
CCTACGGGGTGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	111	0.10692714504522731	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC	109	0.10500052981918717	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	108	0.10403722220616711	No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC	106	0.10211060698012697	No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGTAAGCCTGAA	105	0.10114729936710691	No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	104	0.10018399175408683	No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	104	0.10018399175408683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
