##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	CHILD23_cleaned_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90337
Sequences flagged as poor quality	0
Sequence length	51-301
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.27497038865581	34.0	34.0	34.0	32.0	34.0
2	33.75394356686629	34.0	34.0	34.0	34.0	34.0
3	33.62431783211751	34.0	34.0	34.0	34.0	34.0
4	33.689695252222236	34.0	34.0	34.0	34.0	34.0
5	33.893166698030704	34.0	34.0	34.0	34.0	34.0
6	36.52201202165226	38.0	36.0	38.0	34.0	38.0
7	35.81216998572014	38.0	35.0	38.0	31.0	38.0
8	32.41919700676356	35.0	29.0	37.0	23.0	38.0
9	30.7759279143651	32.0	28.0	37.0	20.0	37.0
10-14	29.658525299711084	31.8	24.0	35.6	19.8	37.6
15-19	35.69693038289959	37.4	35.4	38.0	30.6	38.0
20-24	37.185790982653835	38.0	37.4	38.0	35.6	38.0
25-29	37.1472154266801	38.0	38.0	38.0	36.6	38.0
30-34	37.12012132348872	38.0	38.0	38.0	35.8	38.0
35-39	37.053641365110636	38.0	38.0	38.0	36.0	38.0
40-44	37.01485548557069	38.0	38.0	38.0	35.4	38.0
45-49	37.106386087649575	38.0	37.8	38.0	36.4	38.0
50-54	37.06073882758447	38.0	37.8	38.0	36.0	38.0
55-59	36.70945434758468	38.0	37.2	38.0	34.8	38.0
60-64	36.83848867712648	38.0	37.6	38.0	35.0	38.0
65-69	36.55581902516245	38.0	37.2	38.0	33.6	38.0
70-74	36.91022450515875	38.0	37.4	38.0	35.2	38.0
75-79	35.997936925723145	38.0	37.0	38.0	32.6	38.0
80-84	36.19455557892639	38.0	37.0	38.0	32.8	38.0
85-89	35.86391825619112	38.0	37.0	38.0	31.6	38.0
90-94	36.224177746288646	38.0	37.0	38.0	32.6	38.0
95-99	35.70482959277932	38.0	37.0	38.0	30.4	38.0
100-104	35.89109890839846	38.0	37.0	38.0	31.6	38.0
105-109	36.08100722539494	38.0	36.8	38.0	32.4	38.0
110-114	35.6089757315362	38.0	36.6	38.0	30.0	38.0
115-119	35.22937352315372	38.0	36.2	38.0	27.6	38.0
120-124	35.414698849739786	38.0	36.2	38.0	29.0	38.0
125-129	34.80912702250458	38.0	35.2	38.0	26.0	38.0
130-134	34.48221033305565	38.0	34.8	38.0	24.4	38.0
135-139	34.15554941920698	38.0	34.4	38.0	22.6	38.0
140-144	34.067704220850864	37.6	34.4	38.0	23.4	38.0
145-149	33.788408236633266	37.6	33.8	38.0	22.6	38.0
150-154	33.46932413997642	37.4	33.0	38.0	21.0	38.0
155-159	32.52334303378711	36.6	30.8	37.8	20.0	37.8
160-164	32.49929921843318	35.8	31.2	37.6	20.4	37.8
165-169	31.534246193766858	35.8	28.0	37.8	17.0	37.8
170-174	31.398805008823995	35.8	27.2	37.6	16.8	37.8
175-179	30.65935240973972	35.4	25.6	37.2	16.6	37.6
180-184	29.601330974883204	33.2	24.4	37.2	16.4	37.2
185-189	29.981124272702345	33.6	25.0	37.2	15.6	37.6
190-194	30.19258296369643	33.0	26.2	37.2	17.0	37.6
195-199	27.565060716460486	30.2	21.0	36.4	11.6	37.2
200-204	27.69318338575257	30.4	21.4	35.6	13.0	37.0
205-209	27.640493768660356	30.6	21.4	35.6	12.2	37.4
210-214	28.100654634949432	30.8	21.8	36.2	14.0	37.2
215-219	25.22702060710064	26.2	18.2	34.2	9.4	37.4
220-224	27.868115108798417	29.6	21.0	36.6	13.4	37.4
225-229	25.978760834331514	26.8	18.0	35.2	10.2	37.2
230-234	24.418264154168217	25.8	17.4	32.2	10.4	37.0
235-239	21.879312782024524	22.0	15.4	28.2	9.4	33.2
240-244	22.615490103877764	22.8	15.6	29.8	9.4	34.4
245-249	22.409039586182754	22.6	15.6	29.8	8.0	35.0
250-254	23.32725116600068	24.0	16.6	30.2	10.2	36.2
255-259	22.27901929441843	22.0	16.2	29.6	8.2	33.8
260-264	22.024321774625086	22.2	15.4	29.2	8.6	33.8
265-269	21.137783572080636	21.0	14.4	27.8	8.0	32.6
270-274	20.44609688880221	20.4	12.8	27.6	8.6	32.6
275-279	21.939051750688602	22.2	15.0	29.0	8.8	34.6
280-284	19.56142587100225	19.2	12.6	26.4	7.8	31.0
285-289	20.525410328191022	20.2	13.8	27.0	8.0	32.0
290-294	20.24938671811318	19.8	13.4	27.0	8.0	31.6
295-299	18.33116286557801	18.4	10.6	24.2	7.6	30.4
300-301	17.761038758807267	18.5	11.5	23.5	7.5	29.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-1.312907473872933
1101	2	-0.4927978586662931
1101	3	0.26219373471536045
1101	4	0.21932069623696293
1101	5	0.11686304926181634
1101	6	0.5592314805515457
1101	7	1.3553276784525607
1101	8	1.475218547191595
1101	9	2.2014185312510754
1101	10-14	3.4010556891130435
1101	15-19	1.8724842929496361
1101	20-24	0.3845272547929568
1101	25-29	0.6363404823150063
1101	30-34	0.3810671899062257
1101	35-39	0.5441074998781232
1101	40-44	0.08806815168939153
1101	45-49	0.3412243507255823
1101	50-54	0.3403812662277801
1101	55-59	0.3308700672228184
1101	60-64	0.5150716206390271
1101	65-69	0.6545506171438191
1101	70-74	0.4803330556145724
1101	75-79	0.6228578433397658
1101	80-84	0.44355171721795017
1101	85-89	0.5116753744176918
1101	90-94	0.426163807754115
1101	95-99	0.6074442919097507
1101	100-104	0.5265320774785991
1101	105-109	0.46386530517393965
1101	110-114	0.4665510109124469
1101	115-119	0.42569988277717385
1101	120-124	0.5960582446803926
1101	125-129	0.3995225564134799
1101	130-134	0.09502982339775912
1101	135-139	0.09921803287548414
1101	140-144	0.5909009766003877
1101	145-149	0.24747203342123925
1101	150-154	0.4897890999051242
1101	155-159	0.014097500586522926
1101	160-164	0.3129968907620295
1101	165-169	0.3646273783514644
1101	170-174	0.057766362555415895
1101	175-179	-0.01482619361850368
1101	180-184	0.6192948929416069
1101	185-189	-0.08692824577350322
1101	190-194	0.9064846208392687
1101	195-199	0.010338846836734916
1101	200-204	-0.01809018805769469
1101	205-209	0.5151087032668116
1101	210-214	-0.2952321603657104
1101	215-219	-1.8170615948105784
1101	220-224	-0.30871638453372796
1101	225-229	-1.6237526490695906
1101	230-234	-0.4739242263709329
1101	235-239	-0.5855939775251287
1101	240-244	-1.2643849598299646
1101	245-249	-1.2482963877919708
1101	250-254	-0.6881718520220872
1101	255-259	-0.17346606577538282
1101	260-264	-1.1611047017826799
1101	265-269	-1.9988323656811176
1101	270-274	-0.9564020177266457
1101	275-279	-1.509703814250166
1101	280-284	-0.4532620091733932
1101	285-289	-0.487802035005096
1101	290-294	0.3585739905205898
1101	295-299	-0.21018633232366568
1101	300-301	-0.8564584682643108
1102	1	0.629820869961911
1102	2	0.2334115226837099
1102	3	0.3248100624308137
1102	4	0.2241530989485767
1102	5	0.10455152150538538
1102	6	0.7067990573491798
1102	7	1.5063274564693927
1102	8	2.5376641186274895
1102	9	2.2432879709997273
1102	10-14	4.13353865617416
1102	15-19	1.7808291523318331
1102	20-24	0.4020775852063636
1102	25-29	0.4940424443046538
1102	30-34	0.30181237031023755
1102	35-39	0.5260005039933517
1102	40-44	0.3857544041013199
1102	45-49	0.4305128093086452
1102	50-54	0.4056545616532148
1102	55-59	0.4829900064335888
1102	60-64	0.44561138123872013
1102	65-69	0.6591610366919909
1102	70-74	0.463933622525424
1102	75-79	0.7177505230180685
1102	80-84	0.7073189422576576
1102	85-89	0.5362779391770118
1102	90-94	0.7401846058689685
1102	95-99	0.849006370355184
1102	100-104	0.3930484786192423
1102	105-109	0.5223834821799045
1102	110-114	0.8285918899657787
1102	115-119	0.5156009492700804
1102	120-124	0.6116023218153828
1102	125-129	0.8304591955319367
1102	130-134	0.7300435975024442
1102	135-139	0.7260224405889844
1102	140-144	1.0253224642036898
1102	145-149	1.2394692786002963
1102	150-154	1.0154514571631026
1102	155-159	1.6691136340190766
1102	160-164	1.3486129421717337
1102	165-169	1.474530413147214
1102	170-174	1.4795738371921523
1102	175-179	1.541419801872614
1102	180-184	1.8477566773179745
1102	185-189	2.129963678098882
1102	190-194	2.01705903581011
1102	195-199	2.29241361602746
1102	200-204	1.5832746873384664
1102	205-209	2.439793636276505
1102	210-214	2.0720695007375447
1102	215-219	4.162374029485644
1102	220-224	1.7374805760904088
1102	225-229	1.8051627375439736
1102	230-234	2.2032197059558136
1102	235-239	1.8356840804881465
1102	240-244	1.8326649338764014
1102	245-249	1.874358009519252
1102	250-254	1.9486898639647947
1102	255-259	1.8523985067166286
1102	260-264	1.3814919994910468
1102	265-269	2.611881860115762
1102	270-274	1.0309433688394911
1102	275-279	1.173404498518554
1102	280-284	1.5711310772663758
1102	285-289	1.4559754196032522
1102	290-294	0.6953777590945478
1102	295-299	0.4225174305935582
1102	300-301	1.3409015324369982
1103	1	0.45328265833597925
1103	2	0.22139906037885027
1103	3	0.19598848331683172
1103	4	0.18500013581900276
1103	5	0.044089349932100674
1103	6	1.0554936808976052
1103	7	1.5945132581054864
1103	8	3.776930375437601
1103	9	3.592199185408017
1103	10-14	7.6625135149735755
1103	15-19	1.967963655255815
1103	20-24	0.41932154981874703
1103	25-29	0.4949021434099663
1103	30-34	0.33500741870996364
1103	35-39	0.6275083095947238
1103	40-44	0.3561381031399975
1103	45-49	0.4452376556124378
1103	50-54	0.3119773486777575
1103	55-59	0.4527139363914401
1103	60-64	0.5732233837045229
1103	65-69	0.5734210456888462
1103	70-74	0.33638493123404345
1103	75-79	0.7992819870056636
1103	80-84	0.8746703692582045
1103	85-89	0.8458262639300145
1103	90-94	0.7448729649432906
1103	95-99	0.7236335841636787
1103	100-104	0.34309262983298794
1103	105-109	0.653580896418255
1103	110-114	1.0249631600212652
1103	115-119	0.6431547351970792
1103	120-124	0.7032073674874155
1103	125-129	1.0076332176820344
1103	130-134	0.8025466924935714
1103	135-139	1.6077335524436336
1103	140-144	1.1332289118230534
1103	145-149	1.5831872830838591
1103	150-154	1.294161569540755
1103	155-159	2.2069066336206333
1103	160-164	2.0773488879103397
1103	165-169	2.3279387945250676
1103	170-174	2.330893971317554
1103	175-179	2.5100843801402632
1103	180-184	2.5423405084001125
1103	185-189	2.3815472373761324
1103	190-194	2.143701140269119
1103	195-199	3.672109984743031
1103	200-204	2.0860682121942347
1103	205-209	1.9857395039751182
1103	210-214	2.180482579638216
1103	215-219	4.027861052269145
1103	220-224	3.1023788905807272
1103	225-229	2.0695759371741573
1103	230-234	2.5065792003035803
1103	235-239	2.2773593459776222
1103	240-244	1.3646895152859244
1103	245-249	1.7136454421907565
1103	250-254	2.5504908750735247
1103	255-259	2.5184296246647335
1103	260-264	0.8592071117779803
1103	265-269	2.1486390360911116
1103	270-274	2.0082338230999532
1103	275-279	3.4188961560138225
1103	280-284	2.09274295951613
1103	285-289	1.1982565242984293
1103	290-294	0.9279838065190873
1103	295-299	1.0807013363471256
1103	300-301	0.30871414283620524
1104	1	0.635844366576805
1104	2	0.2416419753586041
1104	3	0.3418517456911303
1104	4	0.27419956118141897
1104	5	0.12101242685518088
1104	6	0.8717565775934375
1104	7	1.5284136107240087
1104	8	3.6268716056844355
1104	9	3.0587167510342432
1104	10-14	4.8606394128448045
1104	15-19	1.9363847078873917
1104	20-24	0.5822149806663361
1104	25-29	0.5721288640577455
1104	30-34	0.41450983479981574
1104	35-39	0.47555911278134033
1104	40-44	0.23436717457789058
1104	45-49	0.4413849717937026
1104	50-54	0.30657053138639156
1104	55-59	0.2981201006855443
1104	60-64	0.5442009205988683
1104	65-69	0.5429423986991679
1104	70-74	0.33618903205681505
1104	75-79	0.7329635855429544
1104	80-84	0.671108269219026
1104	85-89	0.9863708636426907
1104	90-94	0.7369488432245959
1104	95-99	0.9040110165784654
1104	100-104	0.6195651386484542
1104	105-109	0.8455980049464031
1104	110-114	1.1706468481497723
1104	115-119	1.0420805547086402
1104	120-124	0.8704399651105064
1104	125-129	1.1791462123274599
1104	130-134	1.0784706836128066
1104	135-139	1.4885191081443025
1104	140-144	1.0986429120842516
1104	145-149	1.3170419258943582
1104	150-154	1.6096278095825411
1104	155-159	1.2069792261636465
1104	160-164	1.7715343890606476
1104	165-169	1.727092961989129
1104	170-174	1.903022869724726
1104	175-179	2.1219042265964845
1104	180-184	2.2549175179995125
1104	185-189	2.454786003236592
1104	190-194	2.6306992097047903
1104	195-199	3.2155673225338894
1104	200-204	1.6004735873075084
1104	205-209	2.2468900488325616
1104	210-214	1.3757936971908933
1104	215-219	3.9619211827067318
1104	220-224	2.533660221281014
1104	225-229	1.4223482276012795
1104	230-234	2.570656509601136
1104	235-239	2.2213782196824674
1104	240-244	2.236269745808464
1104	245-249	1.0440270794978055
1104	250-254	1.7917992255494504
1104	255-259	3.1740507707278667
1104	260-264	1.8323951124088076
1104	265-269	2.9373593560030287
1104	270-274	1.1505393695387696
1104	275-279	2.6541448989007677
1104	280-284	1.5122378270917665
1104	285-289	1.155981623776352
1104	290-294	0.9342368584531684
1104	295-299	1.8252186866925655
1104	300-301	0.7351910355739228
1105	1	0.5690532281307128
1105	2	0.16382096368545263
1105	3	0.3467312486408005
1105	4	0.27143817044462537
1105	5	0.08210192101860514
1105	6	0.8155403104916417
1105	7	1.361333781428165
1105	8	3.6246122859907004
1105	9	2.09011188094512
1105	10-14	4.638981323225131
1105	15-19	1.8920267312336847
1105	20-24	0.16163948173323206
1105	25-29	0.38098100413556324
1105	30-34	0.3947972704719618
1105	35-39	0.28807637147345133
1105	40-44	0.13048867577030876
1105	45-49	0.396386854560113
1105	50-54	0.1788687615859672
1105	55-59	0.18036875136961328
1105	60-64	0.2923840510118225
1105	65-69	0.2793080319362602
1105	70-74	0.4239887057106344
1105	75-79	0.27216278222928736
1105	80-84	0.17259251674004616
1105	85-89	0.2923266813909109
1105	90-94	0.42482696547889987
1105	95-99	0.11638079190167616
1105	100-104	0.13940322073705858
1105	105-109	0.5018393756003476
1105	110-114	0.7716384384597887
1105	115-119	0.5985478573319583
1105	120-124	0.30296161692423595
1105	125-129	0.5319609481125696
1105	130-134	0.44181322158193126
1105	135-139	0.5585046736018171
1105	140-144	0.4531772411918169
1105	145-149	0.40559784405677846
1105	150-154	0.8432953012669913
1105	155-159	1.3841269965621308
1105	160-164	1.397185444276765
1105	165-169	1.4349654658293716
1105	170-174	1.6244130386955398
1105	175-179	2.2860165193828053
1105	180-184	1.9629317900774588
1105	185-189	1.6623184158137363
1105	190-194	2.5554029025248575
1105	195-199	3.1298363801156377
1105	200-204	2.049890837964483
1105	205-209	1.0578209621815056
1105	210-214	1.7227573405927927
1105	215-219	4.163087834709685
1105	220-224	2.3724829236637284
1105	225-229	1.680805144296908
1105	230-234	2.5197068471866473
1105	235-239	2.5824763061256064
1105	240-244	1.6348628257961515
1105	245-249	1.4530414304563806
1105	250-254	1.4458716350416871
1105	255-259	2.5182930898015137
1105	260-264	1.9566358902639678
1105	265-269	2.6348858450840993
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2119	290-294	-1.0184889203094052
2119	295-299	0.47056781548930715
2119	300-301	-0.2367567414197289
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	16.0
21	64.0
22	210.0
23	426.0
24	872.0
25	1529.0
26	2232.0
27	3169.0
28	4261.0
29	6100.0
30	8623.0
31	12608.0
32	16883.0
33	16994.0
34	11521.0
35	4207.0
36	602.0
37	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.04206471324042198	0.0453856116541395	0.1040548169631491	99.8084948581423
2	0.011069661379058415	0.0453856116541395	0.1095896476526783	99.83395507931412
3	0.06752493441225633	0.11069661379058415	99.71883060097191	0.10294785082524327
4	0.06531100213644465	99.72657936393726	0.05424134075738623	0.15386829316891198
5	0.20146783709886315	0.05756223917110376	0.11401751220430167	99.62695241152572
6	99.82288541793507	0.08745032489456148	0.029888085723457716	0.05977617144691543
7	99.77971373855674	0.061990103722727126	0.08080852806712643	0.0774876296534089
8	99.15759876905366	0.251281313304626	0.4948138636439111	0.0963060539978082
9	43.574615052525544	23.061425550992396	21.263712543033307	12.100246853448752
10-14	58.87178011224636	11.22220131286184	9.504854046514717	20.401164528377077
15-19	39.90170140695396	20.288696768765842	19.885982487795697	19.923619336484496
20-24	52.3278390914022	47.480434373512516	0.17224393105814892	0.01948260402714281
25-29	19.616325536601835	19.26475309120294	60.1257513532661	0.9931700189291209
30-34	11.067668840010183	49.830080697831455	8.55242038146053	30.54983008069783
35-39	64.02979952843243	3.127400732811583	19.988930338620943	12.853869400135052
40-44	39.801631668087275	49.10059001295151	0.18043548047865215	10.91734283848257
45-49	21.681702956706555	20.187077277306088	22.950728937201813	35.18049082878555
50-54	31.198498058368497	28.268339229812124	9.96364699058187	30.569515721237504
55-59	29.273287607543423	30.490083643657652	0.661533216140702	39.575095532658224
60-64	40.00274537327114	6.547272449515021	8.369846059835853	45.08013611737799
65-69	44.60451883586286	29.927933313407063	22.045985409540254	3.4215624411898196
70-74	40.00464951512199	50.9166186954789	8.727582694947527	0.3511490944515786
75-79	27.152624330798968	21.473991382860483	27.784961187447138	23.58842309889341
80-84	31.583177425247143	8.533061739602129	36.26684084090733	23.616919994243393
85-89	36.60581638640112	11.83868217998251	49.19108611661556	2.364415317000808
90-94	5.345673135468443	59.78368444941382	14.18162092747783	20.689021487639902
95-99	3.9308465059019158	7.38697127977805	64.40605535910795	24.276126855212084
100-104	14.170670681752762	33.63970506830813	35.32980537165379	16.85981887828532
105-109	54.63980071962358	40.33988375311375	2.1046221976197064	2.915693329642956
110-114	16.188567695935696	67.71852130686537	14.99728751259369	1.0956234846052502
115-119	44.05563589095153	17.020942072151275	23.39059167941822	15.53283035747898
120-124	30.090234156972883	20.022724655143392	20.46592187679959	29.421119311084137
125-129	21.896206037108833	24.22730545555248	30.94477983937967	22.931708667959015
130-134	20.329213729648515	42.86626024637845	26.113943938819784	10.690582085153249
135-139	22.616833616693967	18.04744942178914	24.775177165048888	34.56053979646801
140-144	31.211789133694772	19.09034845648065	22.52677533563449	27.171087074190087
145-149	12.002930467986857	36.155980818755	32.80925317467365	19.031835538584495
150-154	21.593637188127968	36.75155158003307	20.600892714242526	21.05391851759643
155-159	37.71875	30.939955357142857	8.545758928571429	22.795535714285712
160-164	39.03167089738324	10.842305370204533	8.551658508229137	41.57436522418309
165-169	14.993844447150181	31.021506103692882	26.206420715126427	27.778228734030513
170-174	28.01502109513737	23.11190037213406	19.970365008087413	28.902713524641154
175-179	57.18546594562128	21.70643867436247	8.752420105244719	12.355675274771526
180-184	30.99738015351381	22.16252240658179	0.8571953853932067	45.98290205451119
185-189	25.022831896971354	24.8160699467333	46.27666232435665	3.8844358319386942
190-194	38.65114430651986	27.998690885288823	3.2994366131332256	30.050728195058092
195-199	21.2004716868988	24.178470726407333	37.032414209567825	17.588643377126036
200-204	50.89096942289262	10.390629891301732	19.72485901010889	18.99354167569676
205-209	23.390571034550092	37.968732837842786	37.04853610612773	1.5921600214793934
210-214	58.87520525451559	20.196049161566403	2.6436781609195403	18.28506742299846
215-219	24.01976045593135	19.447582045773242	40.715801932305	15.816855565990403
220-224	60.28641384808109	6.741828948866872	22.49148471389674	10.480272489155304
225-229	25.872070824237603	35.72811034424645	25.941588963468742	12.4582298680472
230-234	50.45213390813076	27.997606832002887	12.62505395555701	8.925205304309342
235-239	44.10528358015909	24.53510726484923	20.023457755463188	11.336151399528493
240-244	40.06371461749725	37.44293640212095	16.08225759875151	6.411091381630284
245-249	23.78481332478184	12.056310289649455	37.93235250293148	26.22652388263723
250-254	41.93564877769485	20.59089158581015	17.179411153257607	20.294048483237397
255-259	22.62277664816545	30.355919473905956	18.16379989375538	28.857503984173217
260-264	31.00012802756084	36.11347121513674	17.49399628332111	15.392404473981308
265-269	32.66063618548272	11.193161417033297	36.10545122621226	20.040751171271722
270-274	37.08515473910568	7.87976604444779	33.8637957076779	21.171283508768628
275-279	4.954560248353904	48.046466214355455	8.232731642590691	38.766241894699945
280-284	28.759919958159948	34.29917911635628	28.47454351138095	8.466357414102827
285-289	22.02492821717567	18.162359697206995	38.95653876272513	20.856173322892197
290-294	40.355025116138535	20.20923820674548	33.097405295161835	6.3383313819541485
295-299	25.98250194030904	35.27481831651732	21.618570521413957	17.124109221759685
300-301	87.27260400894895	10.881110031411719	1.5705859754581817	0.2756999841811485
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.3333333333333333
38	0.3333333333333333
39	0.0
40	0.3333333333333333
41	0.0
42	0.0
43	0.0
44	2.6666666666666665
45	21.833333333333325
46	203.5000000000005
47	1259.833333333328
48	1947.6666666665913
49	3579.0000000004193
50	4615.416666666882
51	3357.3333333336623
52	5067.999999999887
53	5811.24999999937
54	7226.583333331772
55	5555.166666666301
56	2529.3333333333467
57	1450.7499999999868
58	838.5833333333416
59	500.4166666666627
60	350.2499999999988
61	238.25000000000122
62	141.41666666666652
63	75.58333333333337
64	69.16666666666671
65	69.58333333333334
66	39.41666666666667
67	11.25
68	1.8333333333333333
69	0.75
70	1.0
71	0.5
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	2.0
60-64	1.0
65-69	1.0
70-74	0.0
75-79	1.0
80-84	0.0
85-89	0.0
90-94	2.0
95-99	3.0
100-104	0.0
105-109	2.0
110-114	4.0
115-119	11.0
120-124	23.0
125-129	27.0
130-134	22.0
135-139	36.0
140-144	87.0
145-149	85.0
150-154	215.0
155-159	550.0
160-164	407.0
165-169	292.0
170-174	417.0
175-179	792.0
180-184	701.0
185-189	769.0
190-194	1165.0
195-199	1338.0
200-204	872.0
205-209	1174.0
210-214	3065.0
215-219	925.0
220-224	640.0
225-229	2015.0
230-234	4948.0
235-239	3797.0
240-244	3129.0
245-249	2416.0
250-254	4682.0
255-259	3048.0
260-264	4020.0
265-269	5100.0
270-274	2793.0
275-279	3196.0
280-284	4947.0
285-289	4806.0
290-294	4089.0
295-299	1580.0
300-302	22141.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.68234499706654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.6685878962536	4.968064026921416
2	13.602305475504323	2.089952068366229
3	5.763688760806916	1.3283593654870096
4	3.357348703170029	1.0316924405282444
5	2.07492795389049	0.797015619292206
6	1.5994236311239192	0.7372394478452904
7	1.037463976945245	0.557910933504544
8	0.7060518731988472	0.43393072605908983
9	0.547550432276657	0.37858241916379776
>10	4.438040345821326	6.863190055016218
>50	0.9510086455331412	5.201633882019549
>100	0.7492795389048991	10.699934688997864
>500	0.20172910662824206	11.99176417193398
>1k	0.2881844380403458	46.2944308533602
>5k	0.014409221902017292	6.626299301504367
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	5986	6.626299301504367	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	4402	4.872864939061515	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3575	3.957403943013383	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	3120	3.453734350266225	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3054	3.38067458516444	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2792	3.0906494570331096	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2656	2.940102062277915	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	2578	2.8537587035212595	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	1989	2.2017556482947187	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1883	2.0844172376766994	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1700	1.8818424344399305	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1645	1.8209592968551094	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1636	1.8109966016139567	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	1622	1.795499075683275	No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	1528	1.6914442587201257	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	1494	1.6538074100313271	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1354	1.4988321507245095	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	1331	1.473371929552675	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1288	1.4257723856227238	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1132	1.2530856681094125	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	1042	1.1534587156978868	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	991	1.097003442664689	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	957	1.0593665939758903	No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	930	1.0294785082524325	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	894	0.9896277272878222	No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	885	0.9796650320466697	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	795	0.8800380796351439	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	786	0.8700753843939913	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	768	0.8501499939116861	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	760	0.8412942648084395	No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	715	0.7914807886026767	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	676	0.7483091092243488	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	577	0.6387194615716706	No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	575	0.6365055292958588	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	524	0.5800502562626609	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	488	0.5401994752980507	No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	476	0.5269158816431805	No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA	433	0.47931633771322935	No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	343	0.37968938530170365	No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	316	0.3498012995782459	No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA	311	0.3442664688887167	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA	304	0.3365177059233758	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA	299	0.3309828752338466	No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	287	0.31769928157897653	No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	246	0.27231366992483697	No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	241	0.26677883923530776	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC	240	0.2656718730974019	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	233	0.2579231101320611	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA	233	0.2579231101320611	No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC	229	0.2534952455804377	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA	213	0.2357837873739442	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA	208	0.23024895668441503	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT	184	0.20368176937467483	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	174	0.1926121079956164	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC	172	0.19039817571980472	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA	162	0.17932851434074631	No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA	152	0.1682588529616879	No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC	151	0.16715188682378207	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT	149	0.1649379545479704	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA	149	0.1649379545479704	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA	146	0.16161705613425287	No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC	146	0.16161705613425287	No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA	143	0.15829615772053532	No Hit
CCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	142	0.1571891915826295	No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA	142	0.1571891915826295	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGAC	135	0.1494404286172886	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC	134	0.14833346247938275	No Hit
CCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	128	0.1416916656519477	No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	127	0.14058469951404187	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA	126	0.139477733376136	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA	125	0.1383707672382302	No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC	122	0.13504986882451267	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT	120	0.13283593654870096	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT	119	0.13172897041079512	No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC	118	0.1306220042728893	No Hit
CCTACGGGGAGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	115	0.1273011058591718	No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC	113	0.12508717358336008	No Hit
CCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	113	0.12508717358336008	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	110	0.12176627516964256	No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA	110	0.12176627516964256	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT	109	0.12065930903173673	No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	108	0.11955234289383086	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC	108	0.11955234289383086	No Hit
CCTACGGGGTGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA	104	0.11512447834220751	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGAC	104	0.11512447834220751	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	104	0.11512447834220751	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGAC	102	0.11291054606639582	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT	99	0.1095896476526783	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA	98	0.10848268151477246	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC	95	0.10516178310105494	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT	94	0.1040548169631491	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	92	0.10184088468733742	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC	92	0.10184088468733742	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
