FastQCFastQC Report
Thu 10 Oct 2024
CHILD23_cleaned_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD23_cleaned_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90337
Sequences flagged as poor quality0
Sequence length51-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT59866.626299301504367No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT44024.872864939061515No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT35753.957403943013383No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA31203.453734350266225No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT30543.38067458516444No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT27923.0906494570331096No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT26562.940102062277915No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA25782.8537587035212595No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA19892.2017556482947187No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT18832.0844172376766994No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA17001.8818424344399305No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16451.8209592968551094No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16361.8109966016139567No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA16221.795499075683275No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA15281.6914442587201257No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA14941.6538074100313271No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT13541.4988321507245095No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA13311.473371929552675No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA12881.4257723856227238No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT11321.2530856681094125No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA10421.1534587156978868No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA9911.097003442664689No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9571.0593665939758903No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA9301.0294785082524325No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA8940.9896277272878222No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA8850.9796650320466697No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7950.8800380796351439No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA7860.8700753843939913No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT7680.8501499939116861No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA7600.8412942648084395No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA7150.7914807886026767No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA6760.7483091092243488No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA5770.6387194615716706No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA5750.6365055292958588No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5240.5800502562626609No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4880.5401994752980507No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA4760.5269158816431805No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA4330.47931633771322935No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA3430.37968938530170365No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3160.3498012995782459No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA3110.3442664688887167No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA3040.3365177059233758No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA2990.3309828752338466No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2870.31769928157897653No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2460.27231366992483697No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2410.26677883923530776No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC2400.2656718730974019No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2330.2579231101320611No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA2330.2579231101320611No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC2290.2534952455804377No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA2130.2357837873739442No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA2080.23024895668441503No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1840.20368176937467483No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1740.1926121079956164No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC1720.19039817571980472No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1620.17932851434074631No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1520.1682588529616879No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1510.16715188682378207No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT1490.1649379545479704No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1490.1649379545479704No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA1460.16161705613425287No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC1460.16161705613425287No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA1430.15829615772053532No Hit
CCTACGGGGCGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1420.1571891915826295No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1420.1571891915826295No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGAC1350.1494404286172886No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC1340.14833346247938275No Hit
CCTACGGGGCGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1280.1416916656519477No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1270.14058469951404187No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA1260.139477733376136No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA1250.1383707672382302No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1220.13504986882451267No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1200.13283593654870096No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1190.13172897041079512No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1180.1306220042728893No Hit
CCTACGGGGAGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1150.1273011058591718No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC1130.12508717358336008No Hit
CCTACGGGGTGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1130.12508717358336008No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1100.12176627516964256No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA1100.12176627516964256No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1090.12065930903173673No Hit
CCTACGGGGCGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1080.11955234289383086No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC1080.11955234289383086No Hit
CCTACGGGGTGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1040.11512447834220751No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGAC1040.11512447834220751No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1040.11512447834220751No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCGCAATGGGGGCAACCCTGAC1020.11291054606639582No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT990.1095896476526783No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA980.10848268151477246No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC950.10516178310105494No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT940.1040548169631491No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC920.10184088468733742No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC920.10184088468733742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)