##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	CHILD21_cleaned_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1719
Sequences flagged as poor quality	0
Sequence length	15-300
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.54915648632926	23.0	21.0	31.0	12.0	32.0
2	16.650378126817916	12.0	12.0	21.0	12.0	23.0
3	24.641070389761488	23.0	21.0	32.0	11.0	32.0
4	23.545084351367073	23.0	16.0	31.0	11.0	34.0
5	21.077952297847585	23.0	11.0	28.0	9.0	34.0
6	15.70564281559046	11.0	10.0	20.0	9.0	26.0
7	17.79697498545666	17.0	11.0	25.0	9.0	28.0
8	18.034904013961604	18.0	11.0	25.0	9.0	28.0
9	17.070971495055264	16.0	11.0	22.0	9.0	26.0
10-14	14.984409540430482	15.0	9.0	19.0	8.2	22.6
15-19	15.502883638309996	15.4	10.6	19.0	8.4	23.0
20-24	15.37609745680453	15.4	10.6	18.8	8.4	23.6
25-29	15.775673398673097	15.4	11.2	19.6	8.4	23.4
30-34	17.031370455052745	NaN	NaN	NaN	NaN	NaN
35-39	16.5450314641264	NaN	NaN	NaN	NaN	NaN
40-44	18.478209888606607	NaN	NaN	NaN	NaN	NaN
45-49	15.546792108147608	NaN	NaN	NaN	NaN	NaN
50-54	16.984242424242424	NaN	NaN	NaN	NaN	NaN
55-59	15.803174603174606	NaN	NaN	NaN	NaN	NaN
60-64	17.78666666666667	NaN	NaN	NaN	NaN	NaN
65-69	13.48	NaN	NaN	NaN	NaN	NaN
70-74	14.833333333333334	NaN	NaN	NaN	NaN	NaN
75-79	14.0	NaN	NaN	NaN	NaN	NaN
80-84	12.8	NaN	NaN	NaN	NaN	NaN
85-89	12.0	NaN	NaN	NaN	NaN	NaN
90-94	37.2	NaN	NaN	NaN	NaN	NaN
95-99	37.6	NaN	NaN	NaN	NaN	NaN
100-104	38.0	NaN	NaN	NaN	NaN	NaN
105-109	38.0	NaN	NaN	NaN	NaN	NaN
110-114	38.0	NaN	NaN	NaN	NaN	NaN
115-119	38.0	NaN	NaN	NaN	NaN	NaN
120-124	38.0	NaN	NaN	NaN	NaN	NaN
125-129	38.0	NaN	NaN	NaN	NaN	NaN
130-134	38.0	NaN	NaN	NaN	NaN	NaN
135-139	38.0	NaN	NaN	NaN	NaN	NaN
140-144	38.0	NaN	NaN	NaN	NaN	NaN
145-149	38.0	NaN	NaN	NaN	NaN	NaN
150-154	38.0	NaN	NaN	NaN	NaN	NaN
155-159	38.0	NaN	NaN	NaN	NaN	NaN
160-164	38.0	NaN	NaN	NaN	NaN	NaN
165-169	38.0	NaN	NaN	NaN	NaN	NaN
170-174	38.0	NaN	NaN	NaN	NaN	NaN
175-179	38.0	NaN	NaN	NaN	NaN	NaN
180-184	38.0	NaN	NaN	NaN	NaN	NaN
185-189	38.0	NaN	NaN	NaN	NaN	NaN
190-194	38.0	NaN	NaN	NaN	NaN	NaN
195-199	38.0	NaN	NaN	NaN	NaN	NaN
200-204	38.0	NaN	NaN	NaN	NaN	NaN
205-209	38.0	NaN	NaN	NaN	NaN	NaN
210-214	38.0	NaN	NaN	NaN	NaN	NaN
215-219	38.0	NaN	NaN	NaN	NaN	NaN
220-224	38.0	NaN	NaN	NaN	NaN	NaN
225-229	38.0	NaN	NaN	NaN	NaN	NaN
230-234	38.0	NaN	NaN	NaN	NaN	NaN
235-239	38.0	NaN	NaN	NaN	NaN	NaN
240-244	38.0	NaN	NaN	NaN	NaN	NaN
245-249	38.0	NaN	NaN	NaN	NaN	NaN
250-254	38.0	NaN	NaN	NaN	NaN	NaN
255-259	38.0	NaN	NaN	NaN	NaN	NaN
260-264	38.0	NaN	NaN	NaN	NaN	NaN
265-269	38.0	NaN	NaN	NaN	NaN	NaN
270-274	38.0	NaN	NaN	NaN	NaN	NaN
275-279	37.6	NaN	NaN	NaN	NaN	NaN
280-284	37.2	NaN	NaN	NaN	NaN	NaN
285-289	38.0	NaN	NaN	NaN	NaN	NaN
290-294	38.0	NaN	NaN	NaN	NaN	NaN
295-299	37.2	NaN	NaN	NaN	NaN	NaN
300	38.0	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.596021303258144
1101	2	1.2161027568922336
1101	3	5.987218045112783
1101	4	4.204949874686715
1101	5	8.892073934837093
1101	6	-0.93048245614035
1101	7	-0.6517857142857117
1101	8	1.8342418546365913
1101	9	-0.19652255639097405
1101	10	-5.634555137844606
1101	11	2.036904761904763
1101	12	-0.7577694235588996
1101	13	-3.6506578947368435
1101	14	1.4655701754385948
1101	15	1.5101503759398476
1101	16	0.13233082706766552
1101	17	2.7473057644110295
1101	18	-5.277568922305765
1101	19	-4.264724310776941
1101	20	-2.7469298245614056
1101	21	5.015350877192983
1101	22	17.315789473684212
1101	23	12.62280701754386
1101	24	6.508771929824562
1101	25	18.657894736842103
1101	26	18.35087719298246
1101	27	5.6271929824561395
1101	28	4.7631578947368425
1101	29	4.092105263157895
1101	30	9.508771929824562
1101	31	4.074561403508772
1101	32	18.04824561403509
1101	33	17.912280701754387
1101	34	7.4298245614035086
1101	35	5.578947368421052
1101	36	18.719298245614034
1101	37	-3.4342105263157894
1101	38	-2.1578947368421053
1101	39	-2.8157894736842106
1101	40	-2.5789473684210527
1101	41	-3.5526315789473686
1101	42	-2.789473684210526
1101	43	-3.289473684210526
1101	44	-2.0526315789473686
1101	45	-1.894736842105263
1101	46	-2.0526315789473686
1101	47	-1.3421052631578947
1101	48	-1.5
1101	49	-1.2894736842105263
1101	50	-1.631578947368421
1101	51	-1.4473684210526316
1101	52	-1.2105263157894737
1101	53	-1.4473684210526316
1101	54	-0.8157894736842105
1101	55	-0.868421052631579
1101	56	-1.0263157894736843
1101	57	-1.4473684210526316
1101	58	-1.4736842105263157
1101	59	-1.0789473684210527
1101	60	-1.131578947368421
1101	61	-0.8421052631578947
1101	62	-0.6842105263157895
1101	63	-0.9210526315789473
1101	64	-1.1842105263157894
1101	65	-0.631578947368421
1101	66	-0.23684210526315788
1101	67	-0.21052631578947367
1101	68	-0.42105263157894735
1101	69	-0.39473684210526316
1102	1	0.5460213032581471
1102	2	1.1161027568922322
1102	3	0.7372180451127832
1102	4	4.8049498746867165
1102	5	-0.20792606516290846
1102	6	4.96951754385965
1102	7	-0.6517857142857117
1102	8	-1.8157581453634073
1102	9	-2.7965225563909755
1102	10	2.715444862155394
1102	11	9.036904761904763
1102	12	4.042230576441101
1102	13	-2.7006578947368443
1102	14	0.9655701754385948
1102	15	-1.639849624060151
1102	16	5.4656641604009994
1102	17	6.7473057644110295
1102	18	-4.277568922305765
1102	19	5.735275689223059
1102	20	7.2530701754385944
1102	21	14.015350877192983
1102	22	-2.6842105263157894
1102	23	12.62280701754386
1102	24	-2.4912280701754383
1102	25	1.6578947368421044
1102	26	3.3508771929824572
1102	27	5.6271929824561395
1102	28	12.763157894736842
1102	29	13.092105263157894
1102	30	11.508771929824562
1102	31	6.074561403508772
1102	32	16.04824561403509
1102	33	6.912280701754386
1102	34	7.4298245614035086
1102	35	21.57894736842105
1102	36	6.719298245614035
1102	37	14.56578947368421
1102	38	5.842105263157895
1102	39	12.18421052631579
1102	40	13.421052631578947
1102	41	14.447368421052632
1102	42	15.210526315789474
1102	43	14.710526315789474
1102	44	12.947368421052632
1102	45	-1.894736842105263
1102	46	-2.0526315789473686
1102	47	-1.3421052631578947
1102	48	-1.5
1102	49	-1.2894736842105263
1102	50	-1.631578947368421
1102	51	-1.4473684210526316
1102	52	-1.2105263157894737
1102	53	-1.4473684210526316
1102	54	-0.8157894736842105
1102	55	-0.868421052631579
1102	56	-1.0263157894736843
1102	57	-1.4473684210526316
1102	58	-1.4736842105263157
1102	59	-1.0789473684210527
1102	60	-1.131578947368421
1102	61	-0.8421052631578947
1102	62	-0.6842105263157895
1102	63	-0.9210526315789473
1102	64	-1.1842105263157894
1102	65	-0.631578947368421
1102	66	-0.23684210526315788
1102	67	-0.21052631578947367
1102	68	-0.42105263157894735
1102	69	-0.39473684210526316
1103	1	5.196021303258146
1103	2	2.2161027568922336
1103	3	5.787218045112784
1103	4	3.004949874686716
1103	5	-0.3079260651629099
1103	6	-0.7304824561403489
1103	7	4.348214285714288
1103	8	-6.165758145363407
1103	9	-0.7965225563909755
1103	10	-3.2345551378446054
1103	11	0.43690476190476346
1103	12	0.6422305764411007
1103	13	-1.050657894736844
1103	14	-1.1344298245614048
1103	15	0.9101503759398497
1103	16	1.2656641604010002
1103	17	-3.2526942355889705
1103	18	0.12243107769423567
1103	19	0.9852756892230587
1103	20	1.0030701754385944
1103	21	4.515350877192983
1103	22	3.3157894736842106
1103	23	2.6228070175438596
1103	24	8.842105263157894
1103	25	2.9912280701754383
1103	26	9.350877192982457
1103	27	7.1271929824561395
1103	28	12.763157894736842
1103	29	-3.9078947368421053
1103	30	-5.491228070175439
1103	31	-3.9254385964912277
1103	32	-4.951754385964913
1103	33	-4.087719298245614
1103	34	-3.570175438596492
1103	35	-4.421052631578948
1103	36	-3.280701754385965
1103	37	-3.4342105263157894
1103	38	-2.1578947368421053
1103	39	-2.8157894736842106
1103	40	-2.5789473684210527
1103	41	-3.5526315789473686
1103	42	-2.789473684210526
1103	43	-3.289473684210526
1103	44	-2.0526315789473686
1103	45	-1.894736842105263
1103	46	-2.0526315789473686
1103	47	-1.3421052631578947
1103	48	-1.5
1103	49	-1.2894736842105263
1103	50	-1.631578947368421
1103	51	-1.4473684210526316
1103	52	-1.2105263157894737
1103	53	-1.4473684210526316
1103	54	-0.8157894736842105
1103	55	-0.868421052631579
1103	56	-1.0263157894736843
1103	57	-1.4473684210526316
1103	58	-1.4736842105263157
1103	59	-1.0789473684210527
1103	60	-1.131578947368421
1103	61	-0.8421052631578947
1103	62	-0.6842105263157895
1103	63	-0.9210526315789473
1103	64	-1.1842105263157894
1103	65	-0.631578947368421
1103	66	-0.23684210526315788
1103	67	-0.21052631578947367
1103	68	-0.42105263157894735
1103	69	-0.39473684210526316
1104	1	2.796021303258147
1104	2	-4.383897243107768
1104	3	6.6538847117794475
1104	4	6.138283208020049
1104	5	12.292073934837092
1104	6	-0.5304824561403496
1104	7	-6.651785714285712
1104	8	-7.565758145363407
1104	9	2.2034774436090245
1104	10	8.298778195488726
1104	11	1.3702380952380953
1104	12	3.042230576441101
1104	13	3.882675438596488
1104	14	3.1322368421052627
1104	15	3.110150375939849
1104	16	-2.8676691729323345
1104	17	3.7473057644110295
1104	18	-1.2775689223057647
1104	19	5.735275689223059
1104	20	-10.746929824561406
1104	21	-11.984649122807017
1104	22	-10.68421052631579
1104	23	-13.37719298245614
1104	24	-10.491228070175438
1104	25	-9.342105263157896
1104	26	-7.649122807017543
1104	27	-5.3728070175438605
1104	28	-6.2368421052631575
1104	29	-3.9078947368421053
1104	30	-5.491228070175439
1104	31	-3.9254385964912277
1104	32	-4.951754385964913
1104	33	-4.087719298245614
1104	34	-3.570175438596492
1104	35	-4.421052631578948
1104	36	-3.280701754385965
1104	37	-3.4342105263157894
1104	38	-2.1578947368421053
1104	39	-2.8157894736842106
1104	40	-2.5789473684210527
1104	41	-3.5526315789473686
1104	42	-2.789473684210526
1104	43	-3.289473684210526
1104	44	-2.0526315789473686
1104	45	-1.894736842105263
1104	46	-2.0526315789473686
1104	47	-1.3421052631578947
1104	48	-1.5
1104	49	-1.2894736842105263
1104	50	-1.631578947368421
1104	51	-1.4473684210526316
1104	52	-1.2105263157894737
1104	53	-1.4473684210526316
1104	54	-0.8157894736842105
1104	55	-0.868421052631579
1104	56	-1.0263157894736843
1104	57	-1.4473684210526316
1104	58	-1.4736842105263157
1104	59	-1.0789473684210527
1104	60	-1.131578947368421
1104	61	-0.8421052631578947
1104	62	-0.6842105263157895
1104	63	-0.9210526315789473
1104	64	-1.1842105263157894
1104	65	-0.631578947368421
1104	66	-0.23684210526315788
1104	67	-0.21052631578947367
1104	68	-0.42105263157894735
1104	69	-0.39473684210526316
1105	1	-3.203978696741853
1105	2	-1.6338972431077678
1105	3	0.9872180451127832
1105	4	2.0549498746867165
1105	5	-2.2079260651629085
1105	6	2.9695175438596504
1105	7	-1.4017857142857117
1105	8	0.4342418546365927
1105	9	2.4534774436090245
1105	10	4.715444862155394
1105	11	3.536904761904763
1105	12	0.7922305764411011
1105	13	-2.7006578947368443
1105	14	-1.0344298245614052
1105	15	4.360150375939849
1105	16	3.9656641604009994
1105	17	-13.25269423558897
1105	18	-13.277568922305765
1105	19	-12.264724310776941
1105	20	-10.746929824561406
1105	21	-11.984649122807017
1105	22	-10.68421052631579
1105	23	-13.37719298245614
1105	24	-10.491228070175438
1105	25	-9.342105263157896
1105	26	-7.649122807017543
1105	27	-5.3728070175438605
1105	28	-6.2368421052631575
1105	29	-3.9078947368421053
1105	30	-5.491228070175439
1105	31	-3.9254385964912277
1105	32	-4.951754385964913
1105	33	-4.087719298245614
1105	34	-3.570175438596492
1105	35	-4.421052631578948
1105	36	-3.280701754385965
1105	37	-3.4342105263157894
1105	38	-2.1578947368421053
1105	39	-2.8157894736842106
1105	40	-2.5789473684210527
1105	41	-3.5526315789473686
1105	42	-2.789473684210526
1105	43	-3.289473684210526
1105	44	-2.0526315789473686
1105	45	-1.894736842105263
1105	46	-2.0526315789473686
1105	47	-1.3421052631578947
1105	48	-1.5
1105	49	-1.2894736842105263
1105	50	-1.631578947368421
1105	51	-1.4473684210526316
1105	52	-1.2105263157894737
1105	53	-1.4473684210526316
1105	54	-0.8157894736842105
1105	55	-0.868421052631579
1105	56	-1.0263157894736843
1105	57	-1.4473684210526316
1105	58	-1.4736842105263157
1105	59	-1.0789473684210527
1105	60	-1.131578947368421
1105	61	-0.8421052631578947
1105	62	-0.6842105263157895
1105	63	-0.9210526315789473
1105	64	-1.1842105263157894
1105	65	-0.631578947368421
1105	66	-0.23684210526315788
1105	67	-0.21052631578947367
1105	68	-0.42105263157894735
1105	69	-0.39473684210526316
1106	1	5.796021303258147
1106	2	12.616102756892232
1106	3	2.487218045112783
1106	4	7.8049498746867165
1106	5	1.2920739348370915
1106	6	5.46951754385965
1106	7	-0.6517857142857117
1106	8	11.934241854636593
1106	9	4.7034774436090245
1106	10	6.465444862155394
1106	11	-6.963095238095237
1106	12	-6.457769423558899
1106	13	-2.9506578947368443
1106	14	0.4655701754385948
1106	15	1.110150375939849
1106	16	2.4656641604009994
1106	17	2.7473057644110295
1106	18	6.222431077694235
1106	19	2.7352756892230587
1106	20	5.2530701754385944
1106	21	-2.984649122807017
1106	22	9.31578947368421
1106	23	14.62280701754386
1106	24	4.508771929824562
1106	25	8.657894736842104
1106	26	1.3508771929824572
1106	27	3.6271929824561395
1106	28	11.763157894736842
1106	29	-3.9078947368421053
1106	30	-5.491228070175439
1106	31	-3.9254385964912277
1106	32	-4.951754385964913
1106	33	-4.087719298245614
1106	34	-3.570175438596492
1106	35	-4.421052631578948
1106	36	-3.280701754385965
1106	37	-3.4342105263157894
1106	38	-2.1578947368421053
1106	39	-2.8157894736842106
1106	40	-2.5789473684210527
1106	41	-3.5526315789473686
1106	42	-2.789473684210526
1106	43	-3.289473684210526
1106	44	-2.0526315789473686
1106	45	-1.894736842105263
1106	46	-2.0526315789473686
1106	47	-1.3421052631578947
1106	48	-1.5
1106	49	-1.2894736842105263
1106	50	-1.631578947368421
1106	51	-1.4473684210526316
1106	52	-1.2105263157894737
1106	53	-1.4473684210526316
1106	54	-0.8157894736842105
1106	55	-0.868421052631579
1106	56	-1.0263157894736843
1106	57	-1.4473684210526316
1106	58	-1.4736842105263157
1106	59	-1.0789473684210527
1106	60	-1.131578947368421
1106	61	-0.8421052631578947
1106	62	-0.6842105263157895
1106	63	-0.9210526315789473
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2117	13	-1.4506578947368443
2117	14	1.4655701754385948
2117	15	-4.389849624060151
2117	16	-13.534335839599
2117	17	-13.25269423558897
2117	18	-13.277568922305765
2117	19	-12.264724310776941
2117	20	-10.746929824561406
2117	21	-11.984649122807017
2117	22	-10.68421052631579
2117	23	-13.37719298245614
2117	24	-10.491228070175438
2117	25	-9.342105263157896
2117	26	-7.649122807017543
2117	27	-5.3728070175438605
2117	28	-6.2368421052631575
2117	29	-3.9078947368421053
2117	30	-5.491228070175439
2117	31	-3.9254385964912277
2117	32	-4.951754385964913
2117	33	-4.087719298245614
2117	34	-3.570175438596492
2117	35	-4.421052631578948
2117	36	-3.280701754385965
2117	37	-3.4342105263157894
2117	38	-2.1578947368421053
2117	39	-2.8157894736842106
2117	40	-2.5789473684210527
2117	41	-3.5526315789473686
2117	42	-2.789473684210526
2117	43	-3.289473684210526
2117	44	-2.0526315789473686
2117	45	-1.894736842105263
2117	46	-2.0526315789473686
2117	47	-1.3421052631578947
2117	48	-1.5
2117	49	-1.2894736842105263
2117	50	-1.631578947368421
2117	51	-1.4473684210526316
2117	52	-1.2105263157894737
2117	53	-1.4473684210526316
2117	54	-0.8157894736842105
2117	55	-0.868421052631579
2117	56	-1.0263157894736843
2117	57	-1.4473684210526316
2117	58	-1.4736842105263157
2117	59	-1.0789473684210527
2117	60	-1.131578947368421
2117	61	-0.8421052631578947
2117	62	-0.6842105263157895
2117	63	-0.9210526315789473
2117	64	-1.1842105263157894
2117	65	-0.631578947368421
2117	66	-0.23684210526315788
2117	67	-0.21052631578947367
2117	68	-0.42105263157894735
2117	69	-0.39473684210526316
2118	1	-3.203978696741853
2118	2	2.116102756892232
2118	3	2.653884711779451
2118	4	-1.1950501253132835
2118	5	-2.0412593984962406
2118	6	-0.030482456140349612
2118	7	3.014880952380956
2118	8	3.1009085213032606
2118	9	-0.6298558897243076
2118	10	-0.86788847117794
2118	11	-3.963095238095237
2118	12	2.042230576441101
2118	13	0.8826754385964897
2118	14	0.13223684210526088
2118	15	-4.056516290726817
2118	16	2.065664160400999
2118	17	0.9973057644110295
2118	18	2.9724310776942353
2118	19	2.0686090225563927
2118	20	-0.08026315789473948
2118	21	4.348684210526315
2118	22	-2.3508771929824555
2118	23	5.289473684210527
2118	24	5.008771929824562
2118	25	13.657894736842104
2118	26	-7.649122807017543
2118	27	-5.3728070175438605
2118	28	-6.2368421052631575
2118	29	-3.9078947368421053
2118	30	-5.491228070175439
2118	31	-3.9254385964912277
2118	32	-4.951754385964913
2118	33	-4.087719298245614
2118	34	-3.570175438596492
2118	35	-4.421052631578948
2118	36	-3.280701754385965
2118	37	-3.4342105263157894
2118	38	-2.1578947368421053
2118	39	-2.8157894736842106
2118	40	-2.5789473684210527
2118	41	-3.5526315789473686
2118	42	-2.789473684210526
2118	43	-3.289473684210526
2118	44	-2.0526315789473686
2118	45	-1.894736842105263
2118	46	-2.0526315789473686
2118	47	-1.3421052631578947
2118	48	-1.5
2118	49	-1.2894736842105263
2118	50	-1.631578947368421
2118	51	-1.4473684210526316
2118	52	-1.2105263157894737
2118	53	-1.4473684210526316
2118	54	-0.8157894736842105
2118	55	-0.868421052631579
2118	56	-1.0263157894736843
2118	57	-1.4473684210526316
2118	58	-1.4736842105263157
2118	59	-1.0789473684210527
2118	60	-1.131578947368421
2118	61	-0.8421052631578947
2118	62	-0.6842105263157895
2118	63	-0.9210526315789473
2118	64	-1.1842105263157894
2118	65	-0.631578947368421
2118	66	-0.23684210526315788
2118	67	-0.21052631578947367
2118	68	-0.42105263157894735
2118	69	-0.39473684210526316
2119	1	-1.2039786967418529
2119	2	1.4161027568922329
2119	3	1.3872180451127818
2119	4	7.004949874686716
2119	5	-4.30792606516291
2119	6	0.4695175438596504
2119	7	0.9482142857142897
2119	8	-0.5657581453634073
2119	9	0.4034774436090238
2119	10	2.7654448621553946
2119	11	-1.963095238095237
2119	12	1.2422305764411004
2119	13	0.749342105263155
2119	14	-1.9344298245614056
2119	15	1.7101503759398504
2119	16	0.21566416040099945
2119	17	-3.0026942355889705
2119	18	1.4724310776942353
2119	19	3.9852756892230587
2119	20	-0.7469298245614056
2119	21	8.015350877192983
2119	22	5.9824561403508785
2119	23	3.9561403508771917
2119	24	5.508771929824562
2119	25	8.657894736842104
2119	26	10.350877192982457
2119	27	-5.3728070175438605
2119	28	-6.2368421052631575
2119	29	-3.9078947368421053
2119	30	-5.491228070175439
2119	31	-3.9254385964912277
2119	32	-4.951754385964913
2119	33	-4.087719298245614
2119	34	-3.570175438596492
2119	35	-4.421052631578948
2119	36	-3.280701754385965
2119	37	-3.4342105263157894
2119	38	-2.1578947368421053
2119	39	-2.8157894736842106
2119	40	-2.5789473684210527
2119	41	-3.5526315789473686
2119	42	-2.789473684210526
2119	43	-3.289473684210526
2119	44	-2.0526315789473686
2119	45	-1.894736842105263
2119	46	-2.0526315789473686
2119	47	-1.3421052631578947
2119	48	-1.5
2119	49	-1.2894736842105263
2119	50	-1.631578947368421
2119	51	-1.4473684210526316
2119	52	-1.2105263157894737
2119	53	-1.4473684210526316
2119	54	-0.8157894736842105
2119	55	-0.868421052631579
2119	56	-1.0263157894736843
2119	57	-1.4473684210526316
2119	58	-1.4736842105263157
2119	59	-1.0789473684210527
2119	60	-1.131578947368421
2119	61	-0.8421052631578947
2119	62	-0.6842105263157895
2119	63	-0.9210526315789473
2119	64	-1.1842105263157894
2119	65	-0.631578947368421
2119	66	-0.23684210526315788
2119	67	-0.21052631578947367
2119	68	-0.42105263157894735
2119	69	-0.39473684210526316
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
13	3.0
14	52.0
15	226.0
16	400.0
17	392.0
18	319.0
19	185.0
20	91.0
21	30.0
22	11.0
23	5.0
24	2.0
25	2.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	95.46247818499127	1.2216404886561953	0.6399069226294357	2.675974403723095
2	2.4432809773123907	37.75450843513671	52.239674229203025	7.562536358347876
3	1.7452006980802792	14.89237929028505	0.17452006980802792	83.18789994182664
4	17.219313554392087	2.4432809773123907	79.23211169284468	1.1052937754508436
5	0.058173356602675974	77.4869109947644	0.17452006980802792	22.280395578824898
6	49.21465968586388	5.4101221640488655	1.9197207678883073	43.455497382198956
7	3.6067481093659106	40.66317626527051	40.081442699243745	15.648632926119838
8	28.039557882489817	38.976148923792906	4.013961605584642	28.970331588132638
9	74.40372309482257	10.762070971495055	3.5485747527632348	11.28563118091914
10-14	29.354275741710296	19.39499709133217	26.561954624781848	24.688772542175684
15-19	20.42425380138915	28.008259808522624	20.87478881171391	30.692697578374318
20-24	30.178716490658005	27.45735174654752	15.069049553208774	27.2948822095857
25-29	26.027397260273972	33.85518590998043	21.81996086105675	18.297455968688844
30-34	15.521628498727736	45.29262086513995	3.307888040712468	35.87786259541985
35-39	19.06474820143885	35.25179856115108	13.309352517985612	32.37410071942446
40-44	31.21951219512195	31.70731707317073	12.195121951219512	24.878048780487806
45-49	18.446601941747574	33.980582524271846	9.70873786407767	37.86407766990291
50-54	38.59649122807017	28.07017543859649	12.280701754385964	21.052631578947366
55-59	33.33333333333333	36.11111111111111	11.11111111111111	19.444444444444446
60-64	30.76923076923077	30.76923076923077	0.0	38.46153846153847
65-69	33.33333333333333	19.047619047619047	9.523809523809524	38.095238095238095
70-74	0.0	22.22222222222222	11.11111111111111	66.66666666666666
75-79	0.0	0.0	0.0	100.0
80-84	0.0	20.0	0.0	80.0
85-89	20.0	0.0	0.0	80.0
90-94	0.0	0.0	0.0	100.0
95-99	0.0	0.0	0.0	100.0
100-104	0.0	0.0	0.0	100.0
105-109	0.0	0.0	0.0	100.0
110-114	0.0	0.0	0.0	100.0
115-119	0.0	0.0	0.0	100.0
120-124	0.0	0.0	0.0	100.0
125-129	0.0	0.0	0.0	100.0
130-134	0.0	0.0	0.0	100.0
135-139	0.0	0.0	0.0	100.0
140-144	0.0	0.0	0.0	100.0
145-149	0.0	0.0	0.0	100.0
150-154	0.0	0.0	0.0	100.0
155-159	0.0	0.0	0.0	100.0
160-164	0.0	0.0	0.0	100.0
165-169	0.0	0.0	0.0	100.0
170-174	0.0	0.0	0.0	100.0
175-179	0.0	0.0	0.0	100.0
180-184	0.0	0.0	0.0	100.0
185-189	0.0	0.0	0.0	100.0
190-194	0.0	0.0	0.0	100.0
195-199	0.0	0.0	0.0	100.0
200-204	0.0	0.0	0.0	100.0
205-209	0.0	0.0	0.0	100.0
210-214	0.0	0.0	0.0	100.0
215-219	0.0	0.0	0.0	100.0
220-224	0.0	0.0	0.0	100.0
225-229	0.0	0.0	0.0	100.0
230-234	0.0	0.0	0.0	100.0
235-239	0.0	0.0	0.0	100.0
240-244	0.0	0.0	0.0	100.0
245-249	0.0	0.0	0.0	100.0
250-254	0.0	0.0	0.0	100.0
255-259	0.0	0.0	0.0	100.0
260-264	0.0	0.0	0.0	100.0
265-269	0.0	0.0	0.0	100.0
270-274	0.0	0.0	0.0	100.0
275-279	0.0	0.0	0.0	100.0
280-284	0.0	0.0	0.0	100.0
285-289	0.0	0.0	0.0	100.0
290-294	0.0	0.0	0.0	100.0
295-299	0.0	0.0	0.0	100.0
300	0.0	0.0	0.0	100.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.5
20	1.0
21	1.0
22	1.0
23	1.5
24	2.0
25	2.0
26	2.0
27	2.5
28	4.0
29	5.5
30	8.5
31	11.5
32	14.0
33	18.0
34	26.5
35	33.5
36	37.5
37	52.0
38	70.5
39	84.0
40	95.0
41	109.0
42	138.0
43	198.5
44	242.5
45	253.0
46	269.0
47	312.5
48	356.0
49	364.0
50	396.0
51	430.5
52	436.5
53	426.5
54	405.0
55	397.5
56	403.0
57	414.0
58	414.5
59	385.5
60	349.0
61	329.0
62	309.0
63	239.0
64	171.5
65	161.5
66	153.0
67	144.0
68	134.0
69	118.5
70	89.5
71	66.5
72	52.5
73	38.0
74	35.5
75	33.5
76	33.0
77	22.0
78	9.5
79	7.5
80	7.0
81	6.5
82	6.0
83	4.5
84	2.0
85	1.0
86	1.0
87	1.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.25
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
10-19	1113.0
20-29	508.0
30-39	55.0
40-49	28.0
50-59	9.0
60-69	3.0
70-79	2.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-301	1.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.96393251890633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.39667458432304	96.39325189063409
2	1.3064133016627077	2.559627690517743
3	0.17814726840855108	0.5235602094240838
4	0.05938242280285036	0.2326934264107039
5	0.05938242280285036	0.29086678301337987
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTACTAGGCTCGC	5	0.29086678301337987	No Hit
GTCTAGTCGTCTCGC	4	0.2326934264107039	No Hit
GTCTAGTCGGCTAGC	3	0.17452006980802792	No Hit
GTCTACACGGCTCTC	3	0.17452006980802792	No Hit
GTCTACACGGCTAGC	3	0.17452006980802792	No Hit
GTCTACAAGGCTCGC	2	0.11634671320535195	No Hit
GTCTAGTAGGCTCGCT	2	0.11634671320535195	No Hit
GTCTAGTAGGCTCGC	2	0.11634671320535195	No Hit
GTCTACTGGGCTCTC	2	0.11634671320535195	No Hit
GACTACTCGGATATC	2	0.11634671320535195	No Hit
GTCTAGTGGGCTAGC	2	0.11634671320535195	No Hit
GACTACACAGCACACT	2	0.11634671320535195	No Hit
GTCTACACGGCTCGCA	2	0.11634671320535195	No Hit
GTCTAGTAGTCTCGC	2	0.11634671320535195	No Hit
GTCTACTGGGCTCGC	2	0.11634671320535195	No Hit
GTCTACTAGGCTCGCT	2	0.11634671320535195	No Hit
GACTACTCGGCTCTCT	2	0.11634671320535195	No Hit
GTCTAGTAGGATAGC	2	0.11634671320535195	No Hit
GTCTAGCAGGCTCGC	2	0.11634671320535195	No Hit
GACTAGTCGGCTCTC	2	0.11634671320535195	No Hit
GTCTCGTAGGATATC	2	0.11634671320535195	No Hit
GTCTAGTAGACTCTC	2	0.11634671320535195	No Hit
GTCTACACGGCTATC	2	0.11634671320535195	No Hit
GTCTAGAAGGCTATC	2	0.11634671320535195	No Hit
GTCTACTGGGCTAGC	2	0.11634671320535195	No Hit
GTCTCGTGGGCTCTC	2	0.11634671320535195	No Hit
GTCTAGCGGGCTATC	2	0.11634671320535195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
