FastQCFastQC Report
Thu 10 Oct 2024
CHILD21_cleaned_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD21_cleaned_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719
Sequences flagged as poor quality0
Sequence length15-300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACTAGGCTCGC50.29086678301337987No Hit
GTCTAGTCGTCTCGC40.2326934264107039No Hit
GTCTAGTCGGCTAGC30.17452006980802792No Hit
GTCTACACGGCTCTC30.17452006980802792No Hit
GTCTACACGGCTAGC30.17452006980802792No Hit
GTCTACAAGGCTCGC20.11634671320535195No Hit
GTCTAGTAGGCTCGCT20.11634671320535195No Hit
GTCTAGTAGGCTCGC20.11634671320535195No Hit
GTCTACTGGGCTCTC20.11634671320535195No Hit
GACTACTCGGATATC20.11634671320535195No Hit
GTCTAGTGGGCTAGC20.11634671320535195No Hit
GACTACACAGCACACT20.11634671320535195No Hit
GTCTACACGGCTCGCA20.11634671320535195No Hit
GTCTAGTAGTCTCGC20.11634671320535195No Hit
GTCTACTGGGCTCGC20.11634671320535195No Hit
GTCTACTAGGCTCGCT20.11634671320535195No Hit
GACTACTCGGCTCTCT20.11634671320535195No Hit
GTCTAGTAGGATAGC20.11634671320535195No Hit
GTCTAGCAGGCTCGC20.11634671320535195No Hit
GACTAGTCGGCTCTC20.11634671320535195No Hit
GTCTCGTAGGATATC20.11634671320535195No Hit
GTCTAGTAGACTCTC20.11634671320535195No Hit
GTCTACACGGCTATC20.11634671320535195No Hit
GTCTAGAAGGCTATC20.11634671320535195No Hit
GTCTACTGGGCTAGC20.11634671320535195No Hit
GTCTCGTGGGCTCTC20.11634671320535195No Hit
GTCTAGCGGGCTATC20.11634671320535195No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)