FastQCFastQC Report
Thu 10 Oct 2024
CHILD13_cleaned_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD13_cleaned_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6868
Sequences flagged as poor quality0
Sequence length15-300
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG801.1648223645894No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA791.1502620850320326No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA731.0629004076878275No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA711.0337798485730927No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG650.9464181712288876No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG620.902737332556785No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG580.844496214327315No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG580.844496214327315No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA560.8153756552125802No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA540.786255096097845No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA540.786255096097845No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG530.7716948165404777No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG530.7716948165404777No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA520.7571345369831101No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG510.7425742574257426No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA500.7280139778683751No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG480.6988934187536401No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA470.6843331391962726No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA450.6552125800815376No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG430.6260920209668026No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG420.611531741409435No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA420.611531741409435No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA410.5969714618520676No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG400.5824111822947No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG400.5824111822947No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG400.5824111822947No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA390.5678509027373326No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG390.5678509027373326No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG350.5096097845078625No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG340.49504950495049505No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG330.4804892253931275No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG330.4804892253931275No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG320.46592894583576006No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA320.46592894583576006No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG320.46592894583576006No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG310.4513686662783925No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG310.4513686662783925No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG310.4513686662783925No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA280.4076878276062901No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG270.3931275480489225No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG270.3931275480489225No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA270.3931275480489225No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG260.37856726849155503No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG250.36400698893418754No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG240.34944670937682004No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG230.33488642981945255No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA230.33488642981945255No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG200.29120559114735No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA190.2766453115899825No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG190.2766453115899825No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG190.2766453115899825No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG180.262085032032615No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG170.24752475247524752No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG170.24752475247524752No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCATCAG160.23296447291788003No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG160.23296447291788003No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG160.23296447291788003No Hit
GACTACACGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG150.21840419336051253No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG150.21840419336051253No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG150.21840419336051253No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG140.20384391380314504No Hit
GACTACCAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG130.18928363424577752No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG130.18928363424577752No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG120.17472335468841002No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG120.17472335468841002No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG120.17472335468841002No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG120.17472335468841002No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG110.1601630751310425No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG110.1601630751310425No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG110.1601630751310425No Hit
GACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG110.1601630751310425No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG100.145602795573675No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCTAGCTTTCGCGCCTCAG100.145602795573675No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG100.145602795573675No Hit
GACTACAGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG100.145602795573675No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG100.145602795573675No Hit
GACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG100.145602795573675No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG100.145602795573675No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG100.145602795573675No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG100.145602795573675No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG90.1310425160163075No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG90.1310425160163075No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG90.1310425160163075No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG90.1310425160163075No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG90.1310425160163075No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG90.1310425160163075No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA80.11648223645894001No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA80.11648223645894001No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG80.11648223645894001No Hit
GACTACTAGGCTAGCTGATCCTGATAGATACCAACACTTTCGAGCATCAG80.11648223645894001No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG80.11648223645894001No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG80.11648223645894001No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG80.11648223645894001No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG80.11648223645894001No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG80.11648223645894001No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCATCTCAG80.11648223645894001No Hit
GACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG70.10192195690157252No Hit
GACTACTAGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG70.10192195690157252No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG70.10192195690157252No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG70.10192195690157252No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG70.10192195690157252No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG70.10192195690157252No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG70.10192195690157252No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG70.10192195690157252No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA70.10192195690157252No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG70.10192195690157252No Hit
GACTACTGGGGTATCTAATCCTGTTCGATACCCACGCTTTCGTGCCTCAG70.10192195690157252No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGTGCTTCAG70.10192195690157252No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTGAG70.10192195690157252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)