FastQCFastQC Report
Thu 10 Oct 2024
CHILD13_cleaned_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD13_cleaned_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7891
Sequences flagged as poor quality0
Sequence length22-301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1912.4204790267393235No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1772.243061715878849No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1441.8248637688505893No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1391.761500443543277No Hit
CCTACGGGTGGCTGCAGTGGGG1361.7234824483588898No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1291.6347737929286528No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1271.609428462805728No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1201.520719807375491No Hit
CCTACGGGAGGCAGCAGTGGGG1171.4827018121911038No Hit
CCTACGGGAGGCAGCAGTGAGG1141.4446838170067164No Hit
CCTACGGGTGGCAGCAGTGGGG1121.4193384868837917No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1081.368647826637942No Hit
CCTACGGGAGGCTGCAGTGGGG941.191230515777468No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA901.1405398555316182No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT891.1278671904701558No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT881.1151945254086935No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA871.102521860347231No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA801.013813204916994No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT801.013813204916994No Hit
CCTACGGGTGGCTGCAGTGAGG760.9631225446711444No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT740.9377772145482195No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT720.9124318844252947No Hit
CCTACGGGGGGCTGCAGTGGGG690.8744138892409073No Hit
CCTACGGGTGGCAGCAGTGAGG670.8490685591179825No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA630.7983778988721327No Hit
CCTACGGGGGGCAGCAGTGAGG620.7857052338106704No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA610.7730325687492079No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA610.7730325687492079No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA610.7730325687492079No Hit
CCTACGGGCGGCTGCAGTGGGG600.7603599036877455No Hit
CCTACGGGGGGCTGCAGTGAGG600.7603599036877455No Hit
CCTACGGGGGGCAGCAGTGGGG580.7350145735648207No Hit
CCTACGGGAGGCTGCAGTGAGG570.7223419085033582No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA570.7223419085033582No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA540.684323913318971No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA540.684323913318971No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA540.684323913318971No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT520.6589785831960462No Hit
CCTACGGGCGGCAGCAGTGGGG520.6589785831960462No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA470.595615257888734No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA440.5575972627043467No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA440.5575972627043467No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA430.5449245976428844No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT430.5449245976428844No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA420.5322519325814219No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA380.4815612723355722No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA370.46888860727410975No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT370.46888860727410975No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA360.45621594221264733No Hit
CCTACGGGCGGCAGCAGTGAGG360.45621594221264733No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT340.43087061208972244No Hit
CCTACGGGCGGCTGCAGTGAGG310.3928526169053352No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT300.38017995184387277No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGCCGAGAGGCTGAA300.38017995184387277No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC260.3294892915980231No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC250.3168166265365606No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC250.3168166265365606No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC240.3041439614750982No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC240.3041439614750982No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC220.27879863135217337No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC210.26612596629071095No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA190.2407806361677861No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT180.22810797110632366No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA180.22810797110632366No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC170.21543530604486122No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC160.20276264098339883No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA160.20276264098339883No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC150.19008997592193638No Hit
CCTACGGGTGGCTGCAGTGGGGA140.17741731086047396No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA140.17741731086047396No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA140.17741731086047396No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA130.16474464579901155No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC130.16474464579901155No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA120.1520719807375491No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC120.1520719807375491No Hit
CCTACGCGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT120.1520719807375491No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC120.1520719807375491No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC110.13939931567608668No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA110.13939931567608668No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA110.13939931567608668No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT110.13939931567608668No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA110.13939931567608668No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA100.12672665061462424No Hit
CCTACGGGAGGCAGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT100.12672665061462424No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA100.12672665061462424No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT100.12672665061462424No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC100.12672665061462424No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC100.12672665061462424No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC100.12672665061462424No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA90.11405398555316183No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC90.11405398555316183No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA90.11405398555316183No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC90.11405398555316183No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC90.11405398555316183No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT90.11405398555316183No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC90.11405398555316183No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGCAAGTCTGAA90.11405398555316183No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGTCGGCAGACTGAA90.11405398555316183No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGCGAGTCTGAA90.11405398555316183No Hit
CCTACGGGGGGCAGCAGTGAGGA80.10138132049169941No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGCAGGCCTGAA80.10138132049169941No Hit
CCTACGGGTGGCTGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT80.10138132049169941No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC80.10138132049169941No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT80.10138132049169941No Hit
CCTACGCGTGGCAGCAGTGGGG80.10138132049169941No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGCAACCCTGAC80.10138132049169941No Hit
CCTACGGGCGGCAGCAGTGGGGA80.10138132049169941No Hit
CCTACGGGTGGCAGCAGTGGGGAATACTGCACAATGGGGGAAACCCTGAT80.10138132049169941No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGGACAATGGGGGAAACCCTGAT80.10138132049169941No Hit
CCTACGGGTGGCAGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT80.10138132049169941No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAC80.10138132049169941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)