FastQCFastQC Report
Thu 10 Oct 2024
CHILD12_cleaned_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD12_cleaned_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17975
Sequences flagged as poor quality0
Sequence length15-300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1410.78442280945758No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1340.7454798331015299No Hit
GACTACTAGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC1200.6675938803894298No Hit
GACTACAAGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC1200.6675938803894298No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1180.6564673157162726No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1140.6342141863699583No Hit
GACTACAGGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC1130.6286509040333798No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1120.6230876216968011No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1090.6063977746870653No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1060.5897079276773296No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1060.5897079276773296No Hit
GACTACACGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC1040.5785813630041724No Hit
GACTACCAGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC1030.573018080667594No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1010.5618915159944368No Hit
GACTACTCGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC980.545201668984701No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA980.545201668984701No Hit
GACTACAAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG960.5340751043115438No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG960.5340751043115438No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG890.4951321279554937No Hit
GACTACTAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG890.4951321279554937No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA890.4951321279554937No Hit
GACTACTGGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC870.48400556328233657No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA860.47844228094575797No Hit
GACTACAGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG840.4673157162726009No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG820.4561891515994437No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA820.4561891515994437No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG800.44506258692628653No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA780.4339360222531293No Hit
GACTACCGGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC780.4339360222531293No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA750.4172461752433936No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG740.411682892906815No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG740.411682892906815No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG730.4061196105702365No Hit
GACTACTGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG720.4005563282336579No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA720.4005563282336579No Hit
GACTACCGGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG720.4005563282336579No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG690.3838664812239221No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA680.37830319888734354No Hit
GACTACACGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG650.3616133518776078No Hit
GACTACTCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG650.3616133518776078No Hit
GACTACCAGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG650.3616133518776078No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG620.34492350486787204No Hit
GACTACCCGGGTATCTAATCCGGTTCGCGCCCCTAGCTTTCGTCCCTCAC620.34492350486787204No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG600.3337969401947149No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG590.3282336578581363No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG590.3282336578581363No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA570.31710709318497915No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA530.2948539638386648No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA500.27816411682892905No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG490.2726008344923505No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG490.2726008344923505No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG480.2670375521557719No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG470.2614742698191933No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG470.2614742698191933No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG460.2559109874826147No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA460.2559109874826147No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA460.2559109874826147No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG460.2559109874826147No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG460.2559109874826147No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG440.2447844228094576No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG440.2447844228094576No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG430.23922114047287898No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG420.23365785813630044No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA410.22809457579972184No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG410.22809457579972184No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG400.22253129346314326No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG390.21696801112656466No Hit
GACTACCCGGGTATCTAATCCCTTTCGCTCCCCTGGCCTTCGTGCCTCAG380.21140472878998612No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG380.21140472878998612No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG380.21140472878998612No Hit
GACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG370.2058414464534075No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG370.2058414464534075No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA370.2058414464534075No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG360.20027816411682894No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG360.20027816411682894No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG360.20027816411682894No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG350.19471488178025034No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG340.18915159944367177No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG340.18915159944367177No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG320.1780250347705146No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG320.1780250347705146No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG320.1780250347705146No Hit
GACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG310.17246175243393602No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG310.17246175243393602No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG300.16689847009735745No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG280.15577190542420027No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCAG280.15577190542420027No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG280.15577190542420027No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG270.1502086230876217No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG270.1502086230876217No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAA260.1446453407510431No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG250.13908205841446453No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG250.13908205841446453No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG240.13351877607788595No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCCAGCTTTCGCGCCTCAG230.12795549374130735No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG230.12795549374130735No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG230.12795549374130735No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG230.12795549374130735No Hit
GACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG230.12795549374130735No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG230.12795549374130735No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG220.1223922114047288No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG220.1223922114047288No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG220.1223922114047288No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG220.1223922114047288No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG220.1223922114047288No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG210.11682892906815022No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG210.11682892906815022No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG210.11682892906815022No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG210.11682892906815022No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA210.11682892906815022No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG210.11682892906815022No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG210.11682892906815022No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG210.11682892906815022No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG200.11126564673157163No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG200.11126564673157163No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATGAA200.11126564673157163No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG200.11126564673157163No Hit
GACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGGGACTGAG200.11126564673157163No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG190.10570236439499306No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG190.10570236439499306No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG190.10570236439499306No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG190.10570236439499306No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAA180.10013908205841447No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG180.10013908205841447No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCATCAG180.10013908205841447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)