FastQCFastQC Report
Thu 10 Oct 2024
CHILD12_cleaned_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHILD12_cleaned_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20231
Sequences flagged as poor quality0
Sequence length15-301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5302.6197419801295045No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4082.0167070337600714No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3971.962335030398893No Hit
CCTACGGGAGGCAGCAGTGGGG3471.7151895605753544No Hit
CCTACGGGTGGCTGCAGTGGGG3291.626217191438881No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3161.561959369284761No Hit
CCTACGGGTGGCAGCAGTGGGG2961.4631011813553458No Hit
CCTACGGGAGGCTGCAGTGGGG2831.398843359201226No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2621.2950422618753399No Hit
CCTACGGGTGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC2551.2604418961000445No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2531.250556077307103No Hit
CCTACGGGTGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT2121.0478967920518016No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2010.9935247886906233No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1920.9490386041223865No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1840.9094953289506204No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1840.9094953289506204No Hit
CCTACGGGGGGCTGCAGTGGGG1790.8847807819682665No Hit
CCTACGGGGGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1770.8748949631753249No Hit
CCTACGGGGGGCAGCAGTGGGG1750.8650091443823834No Hit
CCTACGGGAGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1550.7661509564529682No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1500.7414364094706144No Hit
CCTACGGGCGGCTGCAGTGGGG1420.7018931342988483No Hit
CCTACGGGTGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1390.687064406109436No Hit
CCTACGGGCGGCAGCAGTGGGG1350.6672927685235529No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1250.6178636745588453No Hit
CCTACGGGCGGCTGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1210.5980920369729623No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1200.5931491275764915No Hit
CCTACGGGAGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1140.563491671197667No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1110.5486629430082547No Hit
CCTACGGGGGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC1100.5437200336117839No Hit
CCTACGGGAGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1090.5387771242153131No Hit
CCTACGGGGGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT1040.5140625772329593No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1040.5140625772329593No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1030.5091196678364885No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1010.49923384904354706No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT980.4844051208541348No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC960.47451930206119325No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT950.46957639266472245No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC950.46957639266472245No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA950.46957639266472245No Hit
CCTACGGGTGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT950.46957639266472245No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC940.4646334832682517No Hit
CCTACGGGCGGCAGCAGGCGCGAAACCTCCGCAATGTGAGAAATCGCGAC940.4646334832682517No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA900.44486184568236864No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA820.40531857051060255No Hit
CCTACGGGAGGCAGCAGTGAGG810.4003756611141318No Hit
CCTACGGGTGGCTGCAGTGAGG810.4003756611141318No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA800.395432751717661No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC790.3904898423211903No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT790.3904898423211903No Hit
CCTACGGGCGGCTGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT780.3855469329247195No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT750.3707182047353072No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC730.36083238594236566No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC720.3558894765458949No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA700.3460036577529534No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC700.3460036577529534No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC670.33117492956354105No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA660.32623202016707037No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC650.3212891107705996No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA650.3212891107705996No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA640.31634620137412883No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT610.3015174731847165No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA590.29163165439177496No Hit
CCTACGGGTGGCAGCAGTGAGG590.29163165439177496No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT580.2866887449953043No Hit
CCTACGGGAGGCTGCAGTGAGG570.2817458355988335No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC570.2817458355988335No Hit
CCTACGGGGGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT570.2817458355988335No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC570.2817458355988335No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA540.2669171074094212No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC540.2669171074094212No Hit
CCTACGGGAGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT540.2669171074094212No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA530.2619741980129504No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC530.2619741980129504No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT530.2619741980129504No Hit
CCTACGGGCGGCAGCAGTCGAGAATCATTCACAATGGGGGAAACCCTGAT520.25703128861647967No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA500.24714546982353816No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT490.2422025604270674No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC490.2422025604270674No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC480.23725965103059662No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCGCAATGGGGGAAACCCTGAC480.23725965103059662No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC460.2273738322376551No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA440.21748801344471355No Hit
CCTACGGGCGGCAGCAGTGAGG440.21748801344471355No Hit
CCTACGGGCGGCTGCAGTGAGG430.2125451040482428No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT420.20760219465177204No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC410.20265928525530127No Hit
CCTACGGGGGGCTGCAGTGAGG410.20265928525530127No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT390.19277346646235974No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT390.19277346646235974No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA390.19277346646235974No Hit
CCTACGGGGGGCAGCAGTGAGG380.18783055706588897No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT370.18288764766941823No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC360.17794473827294746No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC360.17794473827294746No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC350.1730018288764767No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC340.16805891948000592No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTGCGCAATGGGGGGAACCCTGAC330.16311601008353518No Hit
CCTACGGGTGGCTGCAGTGGGGA320.15817310068706442No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT320.15817310068706442No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC300.14828728189412288No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT300.14828728189412288No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGACGAGAGTCTGAA300.14828728189412288No Hit
CCTACGGGTGGCTGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT290.14334437249765214No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT290.14334437249765214No Hit
CCTACGGGAGGCAGCAGTGGGGA290.14334437249765214No Hit
CCTACGCGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT280.13840146310118137No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC280.13840146310118137No Hit
CCTACGGGTGGCTGCAGGCGCG260.12851564430823983No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC260.12851564430823983No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT250.12357273491176908No Hit
CCTACGGGAGGCTGCAGTGGGGA240.11862982551529831No Hit
CCTACGCGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT240.11862982551529831No Hit
CCTACGGGTGGCAGCAGTAGGG240.11862982551529831No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC230.11368691611882754No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT230.11368691611882754No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC230.11368691611882754No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC220.10874400672235678No Hit
CCTACGCGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT210.10380109732588602No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGAAACCCTGAC210.10380109732588602No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC210.10380109732588602No Hit
CCTACGGGAGGCAGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT210.10380109732588602No Hit
CCTACGGGTGGCAGCAGTGGGGA210.10380109732588602No Hit
CCTACGGGTGGCTGCAGTAGGG210.10380109732588602No Hit
CCTACGGGAGGCAGCAGTAGGG210.10380109732588602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)