##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JVMS2_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117324
Sequences flagged as poor quality	0
Sequence length	37-301
%GC	52
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.197487300126145	34.0	34.0	34.0	20.0	34.0
2	30.392460195697385	34.0	34.0	34.0	20.0	34.0
3	31.92594865500665	34.0	34.0	34.0	20.0	34.0
4	32.76301523984863	34.0	34.0	34.0	34.0	34.0
5	31.760006477788007	34.0	34.0	34.0	20.0	34.0
6	32.29621390337868	34.0	34.0	34.0	34.0	34.0
7	31.837953018990145	34.0	34.0	34.0	20.0	34.0
8	31.930287068289523	34.0	34.0	34.0	20.0	34.0
9	32.41963281170093	34.0	34.0	34.0	34.0	34.0
10-14	32.53077631175207	34.0	34.0	34.0	31.2	34.0
15-19	32.56376018546929	34.0	34.0	34.0	31.2	34.0
20-24	32.40424976986806	34.0	34.0	34.0	31.2	34.0
25-29	32.37783062289045	34.0	34.0	34.0	25.6	34.0
30-34	31.900879615423953	34.0	34.0	34.0	20.0	34.0
35-39	31.859763553363912	34.0	34.0	34.0	20.0	34.0
40-44	32.2235112408146	34.0	34.0	34.0	22.8	34.0
45-49	32.19129567596594	34.0	34.0	34.0	25.6	34.0
50-54	32.10820739680023	34.0	34.0	34.0	22.8	34.0
55-59	32.02596791004376	34.0	34.0	34.0	22.8	34.0
60-64	31.94638162941748	34.0	34.0	34.0	20.0	34.0
65-69	31.306428681020815	34.0	34.0	34.0	20.0	34.0
70-74	31.164798685249696	34.0	34.0	34.0	20.0	34.0
75-79	31.90057706896699	34.0	34.0	34.0	20.0	34.0
80-84	31.690650107762906	34.0	34.0	34.0	20.0	34.0
85-89	31.731364801573157	34.0	34.0	34.0	20.0	34.0
90-94	31.675150450926573	34.0	34.0	34.0	20.0	34.0
95-99	30.95716282796597	34.0	34.0	34.0	20.0	34.0
100-104	31.42896670474112	34.0	34.0	34.0	20.0	34.0
105-109	30.83811821266004	34.0	34.0	34.0	20.0	34.0
110-114	31.437093612015616	34.0	34.0	34.0	20.0	34.0
115-119	31.204831477913974	34.0	34.0	34.0	20.0	34.0
120-124	31.02471657261708	34.0	34.0	34.0	20.0	34.0
125-129	30.86975126583357	34.0	34.0	34.0	20.0	34.0
130-134	30.686363093918885	34.0	34.0	34.0	20.0	34.0
135-139	30.68392348739281	34.0	34.0	34.0	20.0	34.0
140-144	30.818124009069674	34.0	34.0	34.0	20.0	34.0
145-149	30.229200406669065	34.0	31.2	34.0	17.8	34.0
150-154	30.347211211416003	34.0	31.2	34.0	20.0	34.0
155-159	30.279222333858797	34.0	31.2	34.0	20.0	34.0
160-164	29.995536536297607	34.0	28.4	34.0	20.0	34.0
165-169	30.20905650083541	34.0	31.2	34.0	20.0	34.0
170-174	29.573698298496264	34.0	28.4	34.0	17.8	34.0
175-179	29.990522385515057	34.0	28.4	34.0	20.0	34.0
180-184	30.095642240938382	34.0	31.2	34.0	20.0	34.0
185-189	29.47345235769881	34.0	22.8	34.0	20.0	34.0
190-194	29.26752141852436	34.0	22.8	34.0	17.8	34.0
195-199	29.863941008482165	34.0	31.2	34.0	17.8	34.0
200-204	28.75331145304974	34.0	20.0	34.0	15.6	34.0
205-209	28.576792123097903	34.0	20.0	34.0	15.6	34.0
210-214	28.271528067857293	34.0	20.0	34.0	15.6	34.0
215-219	28.152343037381183	34.0	20.0	34.0	13.4	34.0
220-224	28.46027023571033	34.0	20.0	34.0	17.8	34.0
225-229	27.719441989226503	34.0	20.0	34.0	13.4	34.0
230-234	28.268154147838686	34.0	20.0	34.0	17.8	34.0
235-239	27.27755307357831	34.0	20.0	34.0	11.2	34.0
240-244	26.024860262393606	31.2	20.0	34.0	9.0	34.0
245-249	26.32170213491576	34.0	20.0	34.0	9.0	34.0
250-254	26.373842166291524	34.0	20.0	34.0	9.0	34.0
255-259	25.508565241115885	28.4	20.0	34.0	11.2	34.0
260-264	26.00093615715163	34.0	20.0	34.0	11.2	34.0
265-269	25.236716458631744	25.6	20.0	34.0	9.0	34.0
270-274	25.339246755289512	25.6	20.0	34.0	9.0	34.0
275-279	23.764591224046107	20.0	20.0	34.0	9.0	34.0
280-284	24.354956875892668	20.0	20.0	34.0	9.0	34.0
285-289	24.41534087543849	20.0	20.0	34.0	9.0	34.0
290-294	23.2068608911587	20.0	20.0	34.0	9.0	34.0
295-299	23.284761017574418	20.0	20.0	34.0	9.0	34.0
300-301	20.61212592023485	20.0	14.5	27.0	9.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.8524135889719844
1101	2	-1.0427722584433639
1101	3	-0.5797774341592792
1101	4	-0.40797654933530936
1101	5	-0.8542370356604643
1101	6	-0.4340262798873127
1101	7	-1.423111198571938
1101	8	-0.8092815696446074
1101	9	-1.0174329875317802
1101	10-14	-0.4143003212589278
1101	15-19	-0.5539960915128361
1101	20-24	-0.6835455272845898
1101	25-29	-0.6942383768565463
1101	30-34	-0.7124514712496648
1101	35-39	-0.8447137928220521
1101	40-44	-0.5802401607320213
1101	45-49	-0.5266477893418227
1101	50-54	-0.806665719924549
1101	55-59	-0.7405382483644836
1101	60-64	-0.6542752230070334
1101	65-69	-1.0913958353741364
1101	70-74	-0.6453082330274036
1101	75-79	-0.265944735655399
1101	80-84	-0.9956039623483228
1101	85-89	-0.7802096999772203
1101	90-94	-0.8059589365980955
1101	95-99	-1.1190335658999828
1101	100-104	-0.8515815463063667
1101	105-109	-1.1723132667700398
1101	110-114	-1.1107900046947208
1101	115-119	-1.3375583653905316
1101	120-124	-1.2909122850491492
1101	125-129	-1.3404493188038487
1101	130-134	-1.4819361373804227
1101	135-139	-1.8086657129302175
1101	140-144	-1.6337613752463795
1101	145-149	-1.4779352615982617
1101	150-154	-1.6415399862395503
1101	155-159	-1.7201575587343498
1101	160-164	-1.9365590011683835
1101	165-169	-1.7510496283696604
1101	170-174	-2.2131510178819447
1101	175-179	-2.121260944705149
1101	180-184	-1.9297960937877292
1101	185-189	-2.5356764312601
1101	190-194	-2.1005509809270535
1101	195-199	-1.8561618225845962
1101	200-204	-2.8720814719159407
1101	205-209	-2.569338246827062
1101	210-214	-1.7994253247812964
1101	215-219	-2.739726022770281
1101	220-224	-3.1181206193144853
1101	225-229	-3.501568636060714
1101	230-234	-3.2445766913363236
1101	235-239	-3.2412849444042813
1101	240-244	-3.16309365191713
1101	245-249	-3.6212727262876854
1101	250-254	-3.981676963870349
1101	255-259	-3.7231285897754667
1101	260-264	-3.7786530060612904
1101	265-269	-4.045193915040496
1101	270-274	-4.167561969253033
1101	275-279	-3.9579282733271874
1101	280-284	-4.376665430316219
1101	285-289	-4.6671991577580805
1101	290-294	-4.533261754471948
1101	295-299	-4.176685753823396
1101	300-301	-3.6021843695527167
1102	1	-0.0390643606481369
1102	2	-0.307334120720963
1102	3	-0.7712351586119297
1102	4	-0.3476268595439933
1102	5	-0.30650078995467567
1102	6	-0.9654829788567021
1102	7	0.0455731064067848
1102	8	-1.2482524971942155
1102	9	-0.4393424373600112
1102	10-14	-0.4065297218639614
1102	15-19	-0.4650508010447041
1102	20-24	-0.6286565358473766
1102	25-29	-0.6415951937565119
1102	30-34	-0.9350428674451159
1102	35-39	-0.9309723171516389
1102	40-44	-0.8795992336951564
1102	45-49	-0.8344619717752977
1102	50-54	-0.9031124204321621
1102	55-59	-0.5040925733401274
1102	60-64	-0.8771434996824148
1102	65-69	-1.075100906388851
1102	70-74	-1.3297670342374737
1102	75-79	-1.022877002486144
1102	80-84	-1.0198592051314854
1102	85-89	-0.7853012243580864
1102	90-94	-1.1732972947994007
1102	95-99	-1.0947731957005225
1102	100-104	-1.0895910833006752
1102	105-109	-1.2658834867362003
1102	110-114	-1.2232137343773353
1102	115-119	-1.3108650361591287
1102	120-124	-1.6556100238585678
1102	125-129	-1.3894981830811375
1102	130-134	-1.6466979688935233
1102	135-139	-1.8005131210212824
1102	140-144	-1.7369429370573854
1102	145-149	-1.730260544888015
1102	150-154	-2.000723188823766
1102	155-159	-1.944361527354566
1102	160-164	-2.4161365020656795
1102	165-169	-2.5098277650665786
1102	170-174	-2.503393544672697
1102	175-179	-2.6247347692449594
1102	180-184	-2.4095017800923983
1102	185-189	-2.4424318022286684
1102	190-194	-3.0055271384412876
1102	195-199	-2.2773021599685883
1102	200-204	-2.7309775391876414
1102	205-209	-2.9997566439967294
1102	210-214	-2.314158955504773
1102	215-219	-2.8125455889908615
1102	220-224	-2.863741805798618
1102	225-229	-2.707508747838393
1102	230-234	-2.945135579712474
1102	235-239	-3.1187653320369577
1102	240-244	-3.4038745574188454
1102	245-249	-3.8786074952975795
1102	250-254	-4.204222213319149
1102	255-259	-4.245530271568011
1102	260-264	-4.454425964067752
1102	265-269	-4.752026197253489
1102	270-274	-4.849121216548323
1102	275-279	-4.229212178367437
1102	280-284	-4.304317586394774
1102	285-289	-5.0214959326132345
1102	290-294	-4.444983028122156
1102	295-299	-4.68134477017567
1102	300-301	-2.248861105515168
1103	1	-0.4572115687699636
1103	2	-0.1871062230898275
1103	3	-0.11000155032089509
1103	4	-0.4322821048908594
1103	5	-0.6697357730342013
1103	6	-0.7136190829176172
1103	7	-0.8450651127133497
1103	8	-0.4934671757052129
1103	9	-0.33356303803682863
1103	10-14	-0.21281673540033808
1103	15-19	-0.4694474804017261
1103	20-24	-0.30084350708257546
1103	25-29	-0.23265378089695332
1103	30-34	-0.55205058741128
1103	35-39	-0.2950136665594236
1103	40-44	-0.0820236203279805
1103	45-49	-0.30392051661455355
1103	50-54	-0.20354071992455047
1103	55-59	-0.1635653948291278
1103	60-64	-0.16068305128986182
1103	65-69	-0.6462885121418154
1103	70-74	-0.7892160613102277
1103	75-79	-0.41643400333217073
1103	80-84	-0.397008634065493
1103	85-89	-0.36832523028025577
1103	90-94	-0.46113570427486295
1103	95-99	-1.019680661859578
1103	100-104	-0.631411091760917
1103	105-109	-0.49314660010337263
1103	110-114	-0.3419610905533119
1103	115-119	-0.6732275573097191
1103	120-124	-1.132436906261269
1103	125-129	-0.9580313895109214
1103	130-134	-1.0827094959662809
1103	135-139	-1.122017985657486
1103	140-144	-1.1387645318120327
1103	145-149	-1.029229453517452
1103	150-154	-1.3136990771486374
1103	155-159	-1.2139549072191969
1103	160-164	-1.6660413244007053
1103	165-169	-1.6910275324100645
1103	170-174	-1.3051649067708375
1103	175-179	-1.495771677028383
1103	180-184	-1.5418383917675271
1103	185-189	-1.5522641837853541
1103	190-194	-2.087435450624021
1103	195-199	-1.4764111912714597
1103	200-204	-2.2935145527240195
1103	205-209	-2.4211848377361562
1103	210-214	-1.8677334055893766
1103	215-219	-2.5713548106490656
1103	220-224	-2.713038548607411
1103	225-229	-2.8969916158586955
1103	230-234	-2.9006215145686483
1103	235-239	-2.9391069141012487
1103	240-244	-3.2782136014120766
1103	245-249	-3.757573231338192
1103	250-254	-3.44599198912287
1103	255-259	-3.5537472766441525
1103	260-264	-3.582109445455231
1103	265-269	-3.9461093190809002
1103	270-274	-3.9618485854146464
1103	275-279	-4.259269813731226
1103	280-284	-4.418253182841472
1103	285-289	-4.663442844626772
1103	290-294	-4.53562917871437
1103	295-299	-4.545321029557233
1103	300-301	-3.3429199132487852
1104	1	0.055193011382709045
1104	2	-0.592024321054204
1104	3	-0.5496286177509226
1104	4	0.012294867119315711
1104	5	-0.6463181521690622
1104	6	-0.8248428424596028
1104	7	-0.5249116457922298
1104	8	-0.557199682066539
1104	9	-0.7295156461793262
1104	10-14	-0.5476529313290968
1104	15-19	-0.6276178048292067
1104	20-24	-0.7020203518662882
1104	25-29	-0.6280497732633705
1104	30-34	-0.7962975657827833
1104	35-39	-0.6390618535823229
1104	40-44	-0.8212610567147536
1104	45-49	-0.6529027049097174
1104	50-54	-0.9687522339194601
1104	55-59	-0.8798782128952851
1104	60-64	-0.7156369316969844
1104	65-69	-1.373761476518407
1104	70-74	-0.9097233768318596
1104	75-79	-0.6306952946807662
1104	80-84	-1.047092126431906
1104	85-89	-0.949698480916382
1104	90-94	-1.1082413022392394
1104	95-99	-1.6130847649130118
1104	100-104	-1.3044152266209643
1104	105-109	-1.636435404174616
1104	110-114	-1.4963941376270995
1104	115-119	-1.924388498785543
1104	120-124	-2.350801251044988
1104	125-129	-1.9348205116483186
1104	130-134	-1.9457382809535595
1104	135-139	-1.9559476930366237
1104	140-144	-1.7115738511504581
1104	145-149	-2.4159104331612227
1104	150-154	-2.06285620183057
1104	155-159	-2.015080066252274
1104	160-164	-2.5564834363599935
1104	165-169	-2.316031508237039
1104	170-174	-2.9804114080431034
1104	175-179	-2.7229225994202437
1104	180-184	-2.5796842887140947
1104	185-189	-2.9516876888744115
1104	190-194	-2.9467110549497235
1104	195-199	-3.0528385131544162
1104	200-204	-2.9512356087036657
1104	205-209	-3.5056671850644
1104	210-214	-2.717765212205151
1104	215-219	-3.196307100725406
1104	220-224	-3.4121718183275185
1104	225-229	-3.4532495675126427
1104	230-234	-3.706994765204783
1104	235-239	-4.0025849675363645
1104	240-244	-4.352664937289941
1104	245-249	-4.657875394188064
1104	250-254	-4.827540176145774
1104	255-259	-4.866485826262476
1104	260-264	-4.8523980904933985
1104	265-269	-5.245799204577082
1104	270-274	-5.625835544702184
1104	275-279	-5.602406157009
1104	280-284	-5.4985784379435145
1104	285-289	-5.738591654150579
1104	290-294	-5.331823906605525
1104	295-299	-5.481308512017581
1104	300-301	-4.0874796298479765
1201	1	-0.8842949021032958
1201	2	-1.2053353897564953
1201	3	-0.6438557169875629
1201	4	-0.3072821048908594
1201	5	-0.4666107730342013
1201	6	-0.5886190829176172
1201	7	-1.0039192793800176
1201	8	-1.6054463423718808
1201	9	-0.7814797047034965
1201	10-14	-0.6076084020670081
1201	15-19	-0.6913224804017304
1201	20-24	-0.7873018404159069
1201	25-29	-0.6857787808969533
1201	30-34	-0.9260089207446107
1201	35-39	-0.918451166559425
1201	40-44	-0.9867111203279784
1201	45-49	-0.9440246832812242
1201	50-54	-0.7691657199245512
1201	55-59	-0.8875237281624635
1201	60-64	-1.1117247179565268
1201	65-69	-1.3233718454751475
1201	70-74	-1.2970285613102348
1201	75-79	-1.3169548366654986
1201	80-84	-1.0944044673988245
1201	85-89	-1.2714502302802586
1201	90-94	-1.2882190376081937
1201	95-99	-1.793118161859578
1201	100-104	-1.6413069250942485
1201	105-109	-1.7837716001033677
1201	110-114	-1.5159194238866434
1201	115-119	-1.5112483906430505
1201	120-124	-1.5360827395946082
1201	125-129	-1.789281389510915
1201	130-134	-1.6988553292996151
1201	135-139	-2.0819138189908237
1201	140-144	-2.0528270318120327
1201	145-149	-2.380791953517452
1201	150-154	-2.0329699104819703
1201	155-159	-2.4144757405525326
1201	160-164	-2.474374657734039
1201	165-169	-2.171756699076731
1201	170-174	-2.4567274067708382
1201	175-179	-2.4515008436950474
1201	180-184	-2.2590258917675285
1201	185-189	-2.3111183504520163
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2102	230-234	3.177480840503815
2102	235-239	3.2674601148842584
2102	240-244	3.60063603626908
2102	245-249	3.69324198605311
2102	250-254	3.9890850036307555
2102	255-259	3.865002723355847
2102	260-264	3.7654675472983925
2102	265-269	4.150222202658227
2102	270-274	3.8420010522665144
2102	275-279	3.7735879761238422
2102	280-284	4.18548323020201
2102	285-289	4.16730896696744
2102	290-294	4.158437850271138
2102	295-299	4.4154003337169385
2102	300-301	3.310357102315912
2103	1	1.4806299612846843
2103	2	1.7699036812817575
2103	3	0.9953177802801996
2103	4	0.640293031721157
2103	5	0.9302471504630674
2103	6	1.260211176645221
2103	7	1.1941681523139742
2103	8	1.7718162942401428
2103	9	1.1178543116899462
2103	10-14	0.8007932372772544
2103	15-19	0.9696833256091963
2103	20-24	1.0273668754310847
2103	25-29	0.9394261371358326
2103	30-34	1.297069972698015
2103	35-39	1.2585980137684523
2103	40-44	1.2350272676501675
2103	45-49	1.181052160981075
2103	50-54	1.1201102363596078
2103	55-59	1.321193826482343
2103	60-64	1.3797370306773544
2103	65-69	1.4209075261095556
2103	70-74	1.6630209605476907
2103	75-79	1.4316790431159205
2103	80-84	1.4848646582842377
2103	85-89	1.4135309855667373
2103	90-94	1.4476757711349713
2103	95-99	1.7923838873207565
2103	100-104	1.5756415038675016
2103	105-109	1.98561023049772
2103	110-114	1.7585426662772932
2103	115-119	1.7284459399580392
2103	120-124	1.9709357030283492
2103	125-129	1.8686250312541048
2103	130-134	2.159751228077436
2103	135-139	1.8650551017742067
2103	140-144	1.8613123124502664
2103	145-149	2.344353196755769
2103	150-154	1.9519771523595573
2103	155-159	2.0146909261141346
2103	160-164	2.5052359980036627
2103	165-169	2.2746913883549666
2103	170-174	2.3919491642674195
2103	175-179	2.4636289377257192
2103	180-184	2.400092960691488
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2103	190-194	2.5912274592120426
2103	195-199	2.5762442049034
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2103	210-214	3.485067140858707
2103	215-219	3.195346009023062
2103	220-224	3.220072790190404
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2103	230-234	3.3267265045570404
2103	235-239	3.5196191787949225
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2103	245-249	3.9244964407929572
2103	250-254	4.358644281368932
2103	255-259	4.320603816252021
2103	260-264	4.007089666566625
2103	265-269	4.534099014252433
2103	270-274	4.446604283437811
2103	275-279	4.490465500476425
2103	280-284	4.634197295300606
2103	285-289	4.672981335701099
2103	290-294	4.627741722924981
2103	295-299	4.442464767786138
2103	300-301	3.593599127460287
2104	1	1.0534267291023767
2104	2	1.1237869506690359
2104	3	0.9725006659911593
2104	4	0.5728154128396312
2104	5	0.8028927730650892
2104	6	0.8427306511249384
2104	7	1.0971445504072186
2104	8	1.1227783916706748
2104	9	0.6279839477787732
2104	10-14	0.6878703213372503
2104	15-19	0.674313601158552
2104	20-24	0.6575784787330292
2104	25-29	0.7813311481810601
2104	30-34	1.0582663452128322
2104	35-39	0.8349176277668171
2104	40-44	0.8046674257713136
2104	45-49	0.920724873456372
2104	50-54	0.8039725779477962
2104	55-59	0.8089190909864712
2104	60-64	0.9667327288519871
2104	65-69	1.3102052821844303
2104	70-74	1.2888202862075033
2104	75-79	1.010350127873508
2104	80-84	1.138529929764296
2104	85-89	1.1669695392232882
2104	90-94	1.2586337992712338
2104	95-99	1.2256096750198573
2104	100-104	1.349595113912848
2104	105-109	1.4801690027335042
2104	110-114	1.3233868704395988
2104	115-119	1.4304515207044624
2104	120-124	1.7114571540224155
2104	125-129	1.5264322629713476
2104	130-134	1.7463574366578278
2104	135-139	1.7389970852644971
2104	140-144	1.70789991854258
2104	145-149	1.931741291163398
2104	150-154	2.098401986681143
2104	155-159	1.9953646849793856
2104	160-164	2.29127250538653
2104	165-169	2.0298390456041204
2104	170-174	2.5231373981937075
2104	175-179	2.1816995109148785
2104	180-184	2.2514794273814047
2104	185-189	2.425450798484153
2104	190-194	2.4074116238440553
2104	195-199	2.2946548548278294
2104	200-204	2.8346990997582466
2104	205-209	3.020426421129091
2104	210-214	3.430759502212041
2104	215-219	3.023813629067245
2104	220-224	2.788933969123086
2104	225-229	3.12820785222641
2104	230-234	3.0820136804668117
2104	235-239	2.7739471639129327
2104	240-244	3.1276596255382785
2104	245-249	3.6428257048320205
2104	250-254	3.591120156267202
2104	255-259	3.698513361653717
2104	260-264	3.5869175935518633
2104	265-269	3.447574191557397
2104	270-274	3.9278012372803843
2104	275-279	3.6759584664106164
2104	280-284	4.171341232052143
2104	285-289	4.175010169557623
2104	290-294	3.9705100056827938
2104	295-299	4.172089230057967
2104	300-301	1.1446400282716809
2201	1	0.9719889732354545
2201	2	0.8279471305687096
2201	3	0.7390202992725996
2201	4	0.6204787352175387
2201	5	1.1441615846893782
2201	6	0.9851656393046042
2201	7	1.202720287015648
2201	8	0.967449152208065
2201	9	0.8046906882504317
2201	10-14	0.7183062320793354
2201	15-19	0.5392288407364845
2201	20-24	0.7160602869553685
2201	25-29	0.8708269101599555
2201	30-34	1.0166443651632484
2201	35-39	0.9184052020042692
2201	40-44	0.7907871859050815
2201	45-49	0.7748057706483209
2201	50-54	0.9480768275009268
2201	55-59	0.02302420544187811
2201	60-64	-0.6803900296096401
2201	65-69	-0.43483017880847896
2201	70-74	-0.4437807632072541
2201	75-79	-0.44947516186875447
2201	80-84	-0.4961829226833743
2201	85-89	-0.7392390175431238
2201	90-94	-0.5305462733805548
2201	95-99	-0.2595731076590404
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2202	1	0.058328971770578164
2202	2	1.0759146102435047
2202	3	0.39685374247189387
2202	4	0.2004318590730989
2202	5	0.4282090467856179
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2202	7	0.3244590989983642
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2202	9	0.33926916917037886
2202	10-14	0.4132699763113763
2202	15-19	0.7167546592379139
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2202	25-29	0.6582977956796228
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2203	4	0.010621192791845147
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2203	9	0.3527393238170049
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2204	1	0.9398878787438463
2204	2	1.2665223450501344
2204	3	0.9744108575980697
2204	4	0.40202958940011513
2204	5	1.0787391348847635
2204	6	0.6386340248171862
2204	7	1.1825448090177737
2204	8	0.7978254117717647
2204	9	0.789336363436469
2204	10-14	0.6736761283197339
2204	15-19	0.7828977083643878
2204	20-24	0.7110579662139287
2204	25-29	0.8980379778507412
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2204	35-39	1.019486218339285
2204	40-44	0.8852099201876698
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2204	50-54	1.0563177054898176
2204	55-59	1.032702445870683
2204	60-64	1.0999465527617005
2204	65-69	1.4012655854640776
2204	70-74	1.561583892649253
2204	75-79	1.235347189117185
2204	80-84	1.5268777609621296
2204	85-89	1.2957487798486653
2204	90-94	1.3038965240860954
2204	95-99	1.6541596926523958
2204	100-104	1.4003856393624758
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2204	135-139	1.9543344544898424
2204	140-144	1.7593046440627376
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2204	220-224	3.073120521374168
2204	225-229	2.997362665909261
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2204	250-254	3.6845990559968342
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2204	290-294	3.9338402043427223
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2301	1	-2.309853845192727
2301	2	0.7520663717611207
2301	3	0.3243861529311367
2301	4	0.287145401884203
2301	5	0.767467817751708
2301	6	0.5624014116623286
2301	7	0.6525847856606326
2301	8	0.8996984747012924
2301	9	0.148864129984851
2301	10-14	0.6716937659546716
2301	15-19	0.5473589220372972
2301	20-24	0.6651117774702726
2301	25-29	0.655650758398437
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2301	35-39	0.7232832507847498
2301	40-44	0.8298115761489839
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2301	50-54	0.8082394291269424
2301	55-59	0.9330513057128798
2301	60-64	0.7066018403090624
2301	65-69	0.8670668401617085
2301	70-74	0.7621812964133454
2301	75-79	0.8063513963968276
2301	80-84	0.7276653157990012
2301	85-89	0.7052054269013155
2301	90-94	0.7816488485706579
2301	95-99	1.0786382744547858
2301	100-104	0.8631265098651042
2301	105-109	1.2331067874305006
2301	110-114	1.017985555788151
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2301	120-124	1.0976316912997
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2301	130-134	1.5140850501041747
2301	135-139	1.5454362826351975
2301	140-144	1.3255266267245531
2301	145-149	1.5057553025801056
2301	150-154	1.627307020412335
2301	155-159	1.5992437716425876
2301	160-164	1.784111114623684
2301	165-169	1.831224330733562
2301	170-174	1.7901010146383811
2301	175-179	1.7007518663320518
2301	180-184	1.7654490404817977
2301	185-189	2.142286121092699
2301	190-194	2.1643048949044363
2301	195-199	2.17352529246838
2301	200-204	2.5231267074385784
2301	205-209	2.534739112128346
2301	210-214	-2.9234583378386994
2301	215-219	1.6891973573726133
2301	220-224	2.4356487820158925
2301	225-229	2.610367801485477
2301	230-234	2.5863915274367706
2301	235-239	2.901484210559996
2301	240-244	2.832262347097405
2301	245-249	3.0631076629707508
2301	250-254	3.156485145023467
2301	255-259	3.1265880892095055
2301	260-264	3.3285655206694287
2301	265-269	3.3186518597808856
2301	270-274	3.432130073121936
2301	275-279	3.556274732339233
2301	280-284	3.700890617971538
2301	285-289	3.846338659438274
2301	290-294	3.7899212955403776
2301	295-299	3.9159109448708627
2301	300-301	3.8574042889259843
2302	1	1.2050513038587596
2302	2	1.2507113582109852
2302	3	1.0642235513051226
2302	4	0.47684729890317357
2302	5	1.206492207995609
2302	6	0.8848946365945807
2302	7	1.113289392706701
2302	8	0.8617580952974926
2302	9	0.7830104714482644
2302	10-14	0.6435094841118527
2302	15-19	0.7343507919559897
2302	20-24	0.7339464658171551
2302	25-29	0.6897970998618561
2302	30-34	1.0112243109627066
2302	35-39	1.0511965299175507
2302	40-44	0.8233075111083323
2302	45-49	0.9720957435480457
2302	50-54	0.8992963396960434
2302	55-59	0.9520214954147761
2302	60-64	1.0930517048076993
2302	65-69	1.3673378428717378
2302	70-74	1.5269509441098208
2302	75-79	1.2182755156380196
2302	80-84	1.3049010881567256
2302	85-89	1.1832542073891261
2302	90-94	1.4618656507386838
2302	95-99	1.662920117273213
2302	100-104	1.5108229868298721
2302	105-109	1.6244347007096351
2302	110-114	1.4770641465740653
2302	115-119	1.8927446649125024
2302	120-124	1.9865205801885857
2302	125-129	1.6582203042560195
2302	130-134	1.9482313915675924
2302	135-139	2.085815686429239
2302	140-144	2.0247136185944754
2302	145-149	2.2260805058321367
2302	150-154	2.4614533618757513
2302	155-159	2.18298360904096
2302	160-164	2.5206964804773406
2302	165-169	2.2209262277525355
2302	170-174	2.546198575613989
2302	175-179	2.54031826199601
2302	180-184	2.3659910459018505
2302	185-189	2.7471641698731837
2302	190-194	2.57568700872557
2302	195-199	2.559975156967024
2302	200-204	2.717706653238043
2302	205-209	3.261026375000977
2302	210-214	3.71394003614504
2302	215-219	3.1314737801368366
2302	220-224	3.167898104509117
2302	225-229	3.3458691564990275
2302	230-234	3.1972316358378556
2302	235-239	3.570860904326935
2302	240-244	3.727113295606891
2302	245-249	4.334652378417907
2302	250-254	4.2139377195492145
2302	255-259	4.276723590564515
2302	260-264	4.721519450208724
2302	265-269	4.678001453276824
2302	270-274	4.823999991821125
2302	275-279	4.788795057542483
2302	280-284	4.9187767968333205
2302	285-289	5.144441640468081
2302	290-294	4.88856628199024
2302	295-299	5.742458900567808
2302	300-301	2.677584472716127
2303	1	1.0944188660126457
2303	2	-0.410498433234757
2303	3	-0.19684484742234432
2303	4	-0.16914804691984386
2303	5	-0.25827743970086914
2303	6	0.4748999388215154
2303	7	0.8574937640982405
2303	8	0.16799115762811923
2303	9	0.5443808025428822
2303	10-14	0.27771768488951665
2303	15-19	0.32378168626493675
2303	20-24	0.8576709856710494
2303	25-29	0.4529893350450749
2303	30-34	0.6201187966466897
2303	35-39	0.681367674020283
2303	40-44	0.6337146043097022
2303	45-49	0.5823113674434168
2303	50-54	0.5813777583363233
2303	55-59	0.5709681196636254
2303	60-64	0.9333839776956481
2303	65-69	0.9262205458291994
2303	70-74	1.1832929966607821
2303	75-79	0.9132987865229012
2303	80-84	0.8718093007171106
2303	85-89	1.0304292987052577
2303	90-94	0.8645889334062922
2303	95-99	1.0304778526331795
2303	100-104	1.159666806789815
2303	105-109	1.1267809361285082
2303	110-114	0.9741847427800252
2303	115-119	1.0592905586323091
2303	120-124	1.217042260405396
2303	125-129	1.3248943351267641
2303	130-134	1.3473403228742988
2303	135-139	1.4091867244874372
2303	140-144	1.619093258043037
2303	145-149	1.4687325030042828
2303	150-154	1.6642900532861482
2303	155-159	2.000741650751813
2303	160-164	1.8179260669036452
2303	165-169	1.700525909618925
2303	170-174	2.0950615425045314
2303	175-179	2.1831911852904575
2303	180-184	2.0091806299716026
2303	185-189	2.058718606069725
2303	190-194	2.4585563972020665
2303	195-199	1.865006381192309
2303	200-204	2.0331611719136617
2303	205-209	2.7915642564667493
2303	210-214	2.682447753830914
2303	215-219	2.6269241748581784
2303	220-224	2.7225139876244704
2303	225-229	2.5338507754456536
2303	230-234	2.7470460578951155
2303	235-239	2.696807940971212
2303	240-244	2.8892229927908204
2303	245-249	2.8340028556183263
2303	250-254	3.1564763079785756
2303	255-259	2.3644592450949773
2303	260-264	3.2948153733853474
2303	265-269	2.9469613330930144
2303	270-274	3.20613148704912
2303	275-279	2.8420662369934035
2303	280-284	3.5414614910715727
2303	285-289	3.344482880010915
2303	290-294	3.5736552415754836
2303	295-299	3.8393880274677734
2303	300-301	2.800673331329506
2304	1	0.6725568236006083
2304	2	0.4505740107884648
2304	3	0.6251327026309674
2304	4	0.3148511830292122
2304	5	0.09458359572147401
2304	6	0.7384202563194435
2304	7	0.8945820830178057
2304	8	1.0045034192085005
2304	9	0.6123526068314717
2304	10-14	0.44126761973135586
2304	15-19	0.485907026864389
2304	20-24	0.7281187999110728
2304	25-29	0.686825669602591
2304	30-34	0.9603969035060764
2304	35-39	0.9493054183633696
2304	40-44	0.9155680576919991
2304	45-49	0.8617924715779992
2304	50-54	0.859546814135399
2304	55-59	1.0412441533089236
2304	60-64	1.1079959904903447
2304	65-69	1.3164868057510155
2304	70-74	1.4432118451384497
2304	75-79	1.091128723734137
2304	80-84	1.1513437160707412
2304	85-89	1.1018396766225607
2304	90-94	1.2989346862791784
2304	95-99	1.3488124693847503
2304	100-104	1.483981165278145
2304	105-109	1.777913799533323
2304	110-114	1.3497637618535947
2304	115-119	1.463801280110804
2304	120-124	1.7954141950102986
2304	125-129	1.560869041915062
2304	130-134	1.735545215659517
2304	135-139	1.7441292668402362
2304	140-144	1.6499545304041305
2304	145-149	1.98845617318554
2304	150-154	1.7957525577287434
2304	155-159	2.178814450183161
2304	160-164	2.2072386483513355
2304	165-169	2.087916325446429
2304	170-174	2.3139455609857507
2304	175-179	2.250820909256813
2304	180-184	2.255330375262446
2304	185-189	2.638455900229186
2304	190-194	2.3249836570054043
2304	195-199	2.4060323259855814
2304	200-204	2.794476478156998
2304	205-209	3.204012426114886
2304	210-214	3.42408085417447
2304	215-219	3.4068184409403983
2304	220-224	3.1520869055251914
2304	225-229	3.0558268446317634
2304	230-234	3.2573499318437023
2304	235-239	3.1444222639187274
2304	240-244	3.4140689826024584
2304	245-249	3.89411273596426
2304	250-254	3.8147780494783987
2304	255-259	3.8227002710397677
2304	260-264	3.879409911947132
2304	265-269	4.375425081464062
2304	270-274	4.188165606229312
2304	275-279	4.226962872463144
2304	280-284	4.480891061027737
2304	285-289	4.7041973688155565
2304	290-294	4.510434059251118
2304	295-299	4.300322750841783
2304	300-301	3.663092776855219
2401	1	-0.10102877307103952
2401	2	1.1350543951897407
2401	3	0.6158889066683493
2401	4	0.49076897037795675
2401	5	0.8546257861055793
2401	6	0.5344487934264706
2401	7	0.7622097528780465
2401	8	0.7459481468754312
2401	9	0.5879759404577953
2401	10-14	0.4624184796534152
2401	15-19	0.635072008845583
2401	20-24	0.6307425144227992
2401	25-29	0.5995706814686343
2401	30-34	0.6649352996854923
2401	35-39	0.6218714140857422
2401	40-44	0.5660778581666435
2401	45-49	0.6412407737080272
2401	50-54	0.6374203015808249
2401	55-59	0.7538573202246326
2401	60-64	0.8502376476348701
2401	65-69	1.0866751975356088
2401	70-74	0.9618928096575132
2401	75-79	0.9722924751624547
2401	80-84	0.954721876687195
2401	85-89	0.8411001729455521
2401	90-94	0.9709879516391133
2401	95-99	1.448985332764078
2401	100-104	1.0992004673788713
2401	105-109	1.4357512493589937
2401	110-114	0.932921301919805
2401	115-119	1.512995560969852
2401	120-124	1.4421766690075408
2401	125-129	1.2436823201664993
2401	130-134	1.5008758534960869
2401	135-139	1.3691446487511065
2401	140-144	1.3553718929191518
2401	145-149	1.6806831808911475
2401	150-154	1.6917444712384615
2401	155-159	1.8453360874044549
2401	160-164	1.924617277749828
2401	165-169	1.7513615804931533
2401	170-174	1.7097880502184104
2401	175-179	1.9921147477028036
2401	180-184	2.1297509899529032
2401	185-189	2.2123359506232525
2401	190-194	2.460397210666301
2401	195-199	2.0816029216317666
2401	200-204	2.717002920394261
2401	205-209	2.724161264414384
2401	210-214	2.864390250324604
2401	215-219	2.7170188452649136
2401	220-224	2.888070322360324
2401	225-229	3.443364566937003
2401	230-234	2.94670711446361
2401	235-239	3.3196632471890695
2401	240-244	3.598164086759965
2401	245-249	3.81131117726396
2401	250-254	4.051672661414763
2401	255-259	4.409357562065523
2401	260-264	4.226039076050149
2401	265-269	4.5201205196287795
2401	270-274	4.359824801682127
2401	275-279	4.597366610999956
2401	280-284	4.203605016083262
2401	285-289	4.979803123115165
2401	290-294	5.0537861438662794
2401	295-299	5.414516592217659
2401	300-301	2.138893996525649
2402	1	0.7660269136148585
2402	2	0.8848576996743986
2402	3	0.4896978060476691
2402	4	0.4090966213964009
2402	5	0.9652997960714913
2402	6	0.668092468572901
2402	7	1.0157284170969483
2402	8	1.3739532172487152
2402	9	1.1000836421799747
2402	10-14	0.7968970179871917
2402	15-19	0.6682261307093782
2402	20-24	0.853187658229082
2402	25-29	0.8523987258781105
2402	30-34	1.0150183489030873
2402	35-39	0.9655596735489773
2402	40-44	0.891058188615105
2402	45-49	0.8734507570981904
2402	50-54	0.8814101608342639
2402	55-59	0.988877863978459
2402	60-64	1.2664934392250444
2402	65-69	1.5158473279665863
2402	70-74	1.5041867164675438
2402	75-79	1.0079774126569916
2402	80-84	1.301107050216352
2402	85-89	1.2363707385544274
2402	90-94	1.2705377374595521
2402	95-99	1.4835163232352748
2402	100-104	1.5146170247702528
2402	105-109	1.6715891722543503
2402	110-114	1.4591779677122716
2402	115-119	1.603855776023618
2402	120-124	1.7377400923837136
2402	125-129	1.7698734207871922
2402	130-134	1.9113750230039024
2402	135-139	1.878769615968526
2402	140-144	1.8577759492177748
2402	145-149	2.084075085777947
2402	150-154	2.018092928271418
2402	155-159	2.187319652401399
2402	160-164	2.1385826593391357
2402	165-169	2.2805468239585025
2402	170-174	2.1483665972942028
2402	175-179	2.190724766061056
2402	180-184	2.2294056800481954
2402	185-189	2.301093709168576
2402	190-194	2.54587671062259
2402	195-199	2.123660793823394
2402	200-204	2.7794952711242225
2402	205-209	2.805199816735392
2402	210-214	3.4602814995596773
2402	215-219	3.070227167670712
2402	220-224	3.2562449879779543
2402	225-229	3.2569802676101425
2402	230-234	3.241134074862245
2402	235-239	3.705820253920429
2402	240-244	4.032804352517459
2402	245-249	4.3780128120222415
2402	250-254	4.284398424156258
2402	255-259	4.479433617664785
2402	260-264	4.3914381493957215
2402	265-269	4.664993323195521
2402	270-274	4.5258970107913115
2402	275-279	5.055461724209152
2402	280-284	5.031513924204603
2402	285-289	4.746609662148298
2402	290-294	4.9113305096325135
2402	295-299	4.289352679288207
2402	300-301	3.0194205160765577
2404	1	0.5743818378234415
2404	2	1.5319585662874609
2404	3	0.38464703026518166
2404	4	0.3899477485889946
2404	5	1.2746895932661673
2404	6	0.9964599005988646
2404	7	1.196630171169435
2404	8	1.233925223562185
2404	9	0.45861873851994517
2404	10-14	0.7169795100209058
2404	15-19	0.6869684994517513
2404	20-24	0.932444038704972
2404	25-29	0.8416479590297854
2404	30-34	1.3045370957389046
2404	35-39	1.1190946209863633
2404	40-44	0.891299410807548
2404	45-49	0.9951637324696918
2404	50-54	0.9202573569985262
2404	55-59	0.9769165190902811
2404	60-64	0.9733027545709447
2404	65-69	1.6122394182611153
2404	70-74	1.5765920888729141
2404	75-79	1.1617997420890802
2404	80-84	1.1288327120883537
2404	85-89	1.3458975261300026
2404	90-94	1.3827745521353982
2404	95-99	1.8022229553565374
2404	100-104	1.4654181206932932
2404	105-109	2.013217868761103
2404	110-114	1.7251462812415639
2404	115-119	1.7120975342653715
2404	120-124	1.9517950076581378
2404	125-129	1.8051439767894522
2404	130-134	1.9525182970740111
2404	135-139	2.159998955734448
2404	140-144	2.1479513564663577
2404	145-149	2.2856400519770546
2404	150-154	2.343924549224983
2404	155-159	2.4551671165903244
2404	160-164	2.659988895379513
2404	165-169	2.4732982459782136
2404	170-174	2.6992421445112207
2404	175-179	2.5422033687591643
2404	180-184	2.5172790532874174
2404	185-189	2.7577690121853458
2404	190-194	2.890741060364988
2404	195-199	2.609034092611328
2404	200-204	3.2853888355543646
2404	205-209	3.2620969846081707
2404	210-214	4.059143883787911
2404	215-219	3.683750500706246
2404	220-224	3.5978245466306795
2404	225-229	3.6323696478775673
2404	230-234	3.7572825605229276
2404	235-239	3.7822415291221887
2404	240-244	4.037777698954219
2404	245-249	4.514885559870603
2404	250-254	4.528418496225115
2404	255-259	4.718574151927278
2404	260-264	4.90246587505759
2404	265-269	4.62713930729273
2404	270-274	4.874335022643958
2404	275-279	5.077308711909794
2404	280-284	4.709820306169519
2404	285-289	5.186408345849419
2404	290-294	5.415389594179402
2404	295-299	5.531839054701411
2404	300-301	3.693839051470704
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	91.0
15	445.0
16	743.0
17	1148.0
18	1362.0
19	1030.0
20	1080.0
21	1301.0
22	1597.0
23	2119.0
24	2856.0
25	3961.0
26	5666.0
27	8014.0
28	10288.0
29	11775.0
30	11640.0
31	12080.0
32	21758.0
33	18354.0
34	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.50308547270805	0.1372268248610685	0.06222085847737888	0.29746684395349626
2	0.700623913265828	98.11888445671815	0.1321127816985442	1.0483788483174799
3	0.6545975248031094	0.4594115441000989	0.16705874330912687	98.71893218778767
4	0.2915004602638846	0.328151102928642	99.0334458422829	0.34690259452456446
5	0.32218471923903036	98.65330196720194	0.664825611128158	0.35968770243087517
6	0.37162046981009855	0.714261361699226	0.13807916538815587	98.77603900310253
7	0.5182230404691283	31.820428897753228	37.319729978521025	30.341618083256623
8	34.38085984112372	32.96938392826702	0.5369745320650506	32.112781698544204
9	99.15703521871058	0.1662064027820395	0.2880910981555351	0.38866728035184617
10-14	39.98005523166616	19.926869182775903	39.86260270703351	0.23047287852442808
15-19	0.30070573795642835	39.93130135351676	39.680372302342235	20.087620606184583
20-24	19.427568102008113	0.9438818996965668	39.33687906992602	40.2916709283693
25-29	20.13228324980396	0.40060004773106955	44.23016603593467	35.23695066653029
30-34	0.44509222324503084	34.62173127407862	0.92069823735979	64.01247826531656
35-39	19.423543089369915	1.5350705228956494	54.84932272790811	24.192063659826328
40-44	37.794040684542225	18.127174567132222	22.17005818165701	21.90872656666854
45-49	1.196205282941673	20.261334287979135	20.327477604179986	58.21498282489921
50-54	38.604682878598034	1.6396041629375815	20.7345658492512	39.02114710921318
55-59	36.13131220145857	23.19835254567833	39.42178853805476	1.248546714808337
60-64	21.892804173272644	32.53925228865136	5.407695323820726	40.160248214255276
65-69	35.609880836700256	39.50834484051893	3.2740073986941476	21.607766924086672
70-74	41.676781659358866	17.87455590636017	2.7228651114581273	37.725797322822835
75-79	0.7956221178516328	0.4016468201539418	40.073987572133625	58.728743489860804
80-84	20.348867935143023	19.561596568601544	1.4722554182443064	58.61728007801113
85-89	58.933089203030484	0.9593025238163432	38.804272962228	1.303335310925172
90-94	0.484852617760881	0.42075114649572937	75.36491808309323	23.729478152650152
95-99	2.5403617641543206	38.17413096476124	19.05927681265663	40.22623045842781
100-104	0.30891453705440103	18.818896295412312	58.8282728404112	22.043916327122083
105-109	20.260326985696505	39.38558057861807	0.7526808394565013	39.60141159622893
110-114	0.33653272414204616	20.30414102323678	43.910871677719626	35.44845457490155
115-119	19.86651209574305	0.8542884907172205	19.906575515283766	59.37262389825596
120-124	0.6043609458376664	54.09482244233425	3.4654005489540887	41.835416062873996
125-129	54.91893550641868	28.135772371583954	15.858635797943979	1.086656324053395
130-134	2.677259321138142	17.472594916207782	39.98244028845662	39.86770547419745
135-139	1.9631109042584929	4.161147281905453	37.464646103016015	56.411095710820035
140-144	1.0148831341527866	1.7532434321564347	53.59820652266567	43.63366691102511
145-149	19.508531818081078	21.993490961847627	18.952757476102555	39.545219743968744
150-154	0.7184456750047737	36.879927166589376	22.058021222619274	40.34360593578658
155-159	21.768187893832348	64.2108690908099	8.323501732180365	5.697441283177391
160-164	21.17263954717496	36.960139120946565	21.225662358918402	20.64155897296007
165-169	16.469493077186684	7.143088524377748	42.062164664242275	34.325253734193296
170-174	22.930615014517365	36.41786820654722	18.991746499380262	21.659770279555154
175-179	17.93688409997613	4.322808333617486	39.76557438537866	37.97473318102772
180-184	42.29549561837215	4.104749889180619	35.81290278582876	17.786851706618474
185-189	23.49854227405248	35.98434862666871	19.914752868565973	20.602356230712836
190-194	51.26294701845616	19.05477174885981	4.560248923745791	25.122032308938238
195-199	18.137675290908316	3.9178210647457483	60.82895017262692	17.11555347171902
200-204	2.0785132773539066	38.4462725374025	18.69792421465411	40.777289970589486
205-209	18.485038097397716	38.58382365226939	3.4576649168063316	39.47347333352656
210-214	3.6552022624049005	21.319029335131763	69.25712259502153	5.7686458074418105
215-219	4.195061404241606	8.315615926400158	31.363987431012934	56.1253352383453
220-224	34.17364988704659	5.07156557691488	20.833042069817996	39.921742466220536
225-229	21.514627600623363	4.461767724819009	17.85610764986513	56.167497024692494
230-234	16.069059595873476	1.4695199931792993	59.0389632534743	23.42245715747293
235-239	18.335780263927468	21.47791197529538	0.6319390091892049	59.55436875158795
240-244	4.589843583478133	52.805965415459546	21.341058953323998	21.263132047738328
245-249	1.6642793806698042	36.12202441852534	22.882647841211377	39.33104835959348
250-254	36.65421867539731	41.0735966168747	4.219016438169293	18.053168269558693
255-259	20.53168269558693	19.78139281085874	20.290055248618785	39.396869244935544
260-264	20.559136484550848	18.298888206807177	19.85897960575677	41.2829957028852
265-269	1.6433081701592152	19.49614537914429	20.528818854134872	58.33172759656162
270-274	18.553633395005413	19.934008031580653	59.18030131216588	2.3320572612480497
275-279	21.71530886302596	19.116511062795755	1.2825169459010104	57.88566312827727
280-284	58.28352265653911	0.6904996188523961	19.78249858881592	21.243479135792573
285-289	41.44632722113076	1.8490094957432874	19.849139107181838	36.85552417594412
290-294	21.37533598024368	37.56941317724748	18.7398523733508	22.315398469158037
295-299	36.717275646603944	19.338397643935284	40.864789049622736	3.0795376598380377
300-301	11.233733612069944	2.178845187742071	0.6328293386487757	85.95459186153921
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.25
2	0.0
3	0.0
4	0.0
5	0.0
6	0.25
7	0.25
8	0.25
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.25
15	0.0
16	0.0
17	0.25
18	0.0
19	0.0
20	0.0
21	0.5
22	0.75
23	0.25
24	0.0
25	0.25
26	1.0
27	1.0
28	0.5
29	0.5
30	0.25
31	0.75
32	1.75
33	1.5
34	1.0
35	4.5
36	5.666666666666666
37	7.166666666666666
38	9.0
39	10.75
40	14.0
41	16.333333333333336
42	18.5
43	25.333333333333332
44	35.83333333333333
45	45.416666666666664
46	97.0
47	725.0
48	984.1666666666666
49	351.9166666666666
50	358.91666666666663
51	901.8333333333345
52	6648.249999999985
53	18620.00000000001
54	5773.416666666668
55	2244.5833333333326
56	946.0833333333338
57	538.1666666666666
58	118.16666666666667
59	29.499999999999996
60	16.25
61	6.75
62	4.25
63	9.333333333333332
64	6.083333333333333
65	2.5
66	1.25
67	2.1666666666666665
68	1.0833333333333333
69	1.0
70	2.0
71	2.5
72	1.8333333333333333
73	1.3333333333333333
74	1.0
75	0.75
76	0.3333333333333333
77	0.75
78	1.25
79	1.0
80	0.25
81	1.0
82	0.5
83	1.0
84	0.25
85	1.75
86	0.25
87	0.75
88	1.5
89	0.5
90	0.8333333333333333
91	1.1666666666666665
92	1.3333333333333333
93	1.25
94	1.25
95	1.25
96	2.5
97	2.5833333333333335
98	3.8333333333333335
99	6.75
100	21.166666666666657
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	1.704826363434884E-4
140-144	0.0
145-149	0.0
150-154	1.704899027355105E-4
155-159	0.0
160-164	0.0
165-169	1.7049135608824634E-4
170-174	1.7049135608824634E-4
175-179	0.0
180-184	0.0
185-189	1.704939721856134E-4
190-194	0.0
195-199	0.0
200-204	0.0
205-209	3.4099143259025617E-4
210-214	1.9109159882357956
215-219	3.4099143259025617E-4
220-224	0.0
225-229	0.0
230-234	0.0
235-239	1.7051752067524938E-4
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	1.70520428347316E-4
270-274	3.4104260133654594E-4
275-279	0.0
280-284	1.7053554984071978E-4
285-289	1.705410756707807E-4
290-294	0.0011938241769861397
295-299	0.0
300-301	8.102742778430498E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-39	1.0
40-49	2.0
50-59	3.0
60-69	0.0
70-79	1.0
80-89	3.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	5.0
150-159	1.0
160-169	0.0
170-179	0.0
180-189	3.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	15.0
230-239	0.0
240-249	2.0
250-259	0.0
260-269	0.0
270-279	8.0
280-289	9.0
290-299	330.0
300-302	116941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.235109610991783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.5851407475773	7.072721693771096
2	7.946469773880942	1.4677303876444716
3	2.7964928472542687	0.7747775391224302
4	2.076603599446239	0.7671064743786438
5	1.2921089063221043	0.5966383689611674
6	1.0336871250576833	0.5727728342027206
7	0.904476234425473	0.5847056015819441
8	0.6552838024919243	0.484129419385633
9	0.6460544531610521	0.5369745320650506
>10	4.753114905399169	8.560908254065664
>50	0.5445316105214583	3.3752684872660326
>100	0.63682510383018	12.830281954246361
>500	0.046146746654360866	2.2075619651563194
>1k	0.0	0.0
>5k	0.08306414397784956	60.16842248815246
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	9145	7.7946541202141075	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	9023	7.690668575909447	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	8591	7.3224574682076975	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7604	6.481197367972452	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7600	6.477788005864103	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7391	6.29964883570284	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7372	6.283454365688179	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7175	6.1155432818519655	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6691	5.703010466741673	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	528	0.4500357983021377	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	526	0.4483311172479629	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	517	0.44066005250417645	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	512	0.43639834986873954	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	507	0.4321366472333026	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	491	0.41849919879990455	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	466	0.39719068562272003	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	460	0.3920766424601957	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	459	0.3912243019331083	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	438	0.3733251508642733	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	438	0.3733251508642733	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	428	0.3648017455933995	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	419	0.357130680849613	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	411	0.350311956632914	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	409	0.3486072755787392	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG	380	0.3238894002932051	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	374	0.31877535713068084	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	360	0.3068425897514575	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	287	0.2446217312740786	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	265	0.2258702396781562	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	265	0.2258702396781562	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	261	0.22246087756980668	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	243	0.2071187480822338	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	236	0.20115236439262213	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	234	0.19944768333844737	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	229	0.19518598070301046	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	228	0.19433364017592308	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	220	0.18751491595922404	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	215	0.18325321332378713	No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	205	0.1747298080529133	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	204	0.17387746752582592	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	202	0.17217278647165116	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	198	0.16876342436330163	No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	198	0.16876342436330163	No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	197	0.16791108383621425	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	197	0.16791108383621425	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	195	0.1662064027820395	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	193	0.16450172172786473	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	191	0.16279704067368994	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG	190	0.16194470014660256	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG	189	0.16109235961951518	No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	187	0.15938767856534042	No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	185	0.15768299751116566	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	180	0.15342129487572875	No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	177	0.1508642732944666	No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	175	0.14915959224029185	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	169	0.14404554907776754	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	167	0.14234086802359278	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	156	0.1329651222256316	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	156	0.1329651222256316	No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	155	0.1321127816985442	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	152	0.12955576011728206	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	151	0.12870341959019468	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	138	0.11762299273805871	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	136	0.11591831168388395	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	134	0.11421363062970918	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG	133	0.1133612901026218	No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG	124	0.10569022535883536	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	123	0.10483788483174798	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	122	0.1039855443046606	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	121	0.10313320377757322	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	119	0.10142852272339845	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	6.818724216699055E-4	0.0	0.0	0.0
175-179	0.0	8.523405270873819E-4	0.0	0.0	0.0
180-184	0.0	8.523405270873819E-4	0.0	0.0	0.0
185-189	0.0	8.523405270873819E-4	0.0	0.0	0.0
190-194	0.0	8.523405270873819E-4	0.0	0.0	0.0
195-199	0.0	8.523405270873819E-4	0.0	0.0	0.0
200-204	0.0	8.523405270873819E-4	0.0	0.0	0.0
205-209	0.0	8.523405270873819E-4	0.0	0.0	0.0
210-214	0.0	8.523405270873819E-4	0.0	0.0	0.0
215-219	0.0	8.523405270873819E-4	0.0	0.0	0.0
220-224	0.0	8.523405270873819E-4	0.0	0.0	0.0
225-229	0.0	8.523405270873819E-4	0.0	0.0	0.0
230-234	0.0	8.523405270873819E-4	0.0	0.0	0.0
235-239	0.0	8.523405270873819E-4	0.0	0.0	0.0
240-244	0.0	8.523405270873819E-4	0.0	0.0	0.0
245-249	0.0	8.523405270873819E-4	0.0	0.0	0.0
250-254	0.0	8.523405270873819E-4	0.0	0.0	0.0
255-259	0.0	8.523405270873819E-4	0.0	0.0	0.0
260-264	0.0	8.523405270873819E-4	0.0	0.0	0.0
265-269	0.0	8.523405270873819E-4	0.0	0.0	0.0
270-274	0.0	8.523405270873819E-4	0.0	0.0	0.0
275-279	0.0	8.523405270873819E-4	0.0	0.0	0.0
280-284	0.0	8.523405270873819E-4	0.0	8.523405270873819E-4	0.0
285-289	0.0	8.523405270873819E-4	0.0	8.523405270873819E-4	0.0
290	0.0	8.523405270873819E-4	0.0	8.523405270873819E-4	0.0
>>END_MODULE
