FastQCFastQC Report
Mon 14 Oct 2024
JVFS2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJVFS2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150055
Sequences flagged as poor quality0
Sequence length82-301
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1642810.947985738562526No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT138829.251274532671355No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT125218.344273766285696No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT109087.269334577321648No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT107077.13538369264603No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT95556.367665189430542No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT93056.201059611475792No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT29151.942621038952384No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC29041.935290393522375No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC24641.6420645763220152No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC21331.4214787911099263No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC19851.3228482889607145No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC18881.2582053247142713No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC17071.1375828862750326No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC16511.1002632368131684No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT14440.9623138182666355No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT13150.8763453400419846No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12780.8516877145046816No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT12750.8496884475692246No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11030.7350638099363567No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT10760.7170704075172437No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10740.7157375628936057No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10080.6717536903135517No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT9660.6437639532171537No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9420.6277698177334977No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8930.5951151244543668No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8620.5744560327879777No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT8510.5671253873579687No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT8290.5524640964979508No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7710.5138116024124488No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7630.5084802239178967No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT7520.5011495784878878No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT6990.4658291959614808No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6850.45649928359601477No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT6350.4231781680050648No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC5170.3445403352104228No Hit
CCTACGGGCGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT4120.27456599246942787No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3620.2412448768784779No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2850.18993035886841492No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2540.16927126720202593No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2460.16393988870747392No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2440.16260704408383592No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2200.14661290860017992No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1980.13195161774016195No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1850.12328812768651494No Hit
CCTACGGGCGGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCA1770.11795674919196295No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1760.11729032688014396No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1710.11395821532104895No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC1660.11062610376195395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)