##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JVFS2_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	150055
Sequences flagged as poor quality	0
Sequence length	82-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29152644030522	34.0	34.0	34.0	20.0	34.0
2	33.118969711105926	34.0	34.0	34.0	34.0	34.0
3	33.63192829295925	34.0	34.0	34.0	34.0	34.0
4	33.40719736096764	34.0	34.0	34.0	34.0	34.0
5	33.25837859451534	34.0	34.0	34.0	34.0	34.0
6	33.15960814368065	34.0	34.0	34.0	34.0	34.0
7	33.53999533504382	34.0	34.0	34.0	34.0	34.0
8	33.50489487188031	34.0	34.0	34.0	34.0	34.0
9	33.412382126553595	34.0	34.0	34.0	34.0	34.0
10-14	33.24568458231982	34.0	34.0	34.0	31.2	34.0
15-19	33.42405651261204	34.0	34.0	34.0	34.0	34.0
20-24	33.631528439572165	34.0	34.0	34.0	34.0	34.0
25-29	33.526073772949914	34.0	34.0	34.0	34.0	34.0
30-34	33.448566192396115	34.0	34.0	34.0	34.0	34.0
35-39	33.56300156609244	34.0	34.0	34.0	34.0	34.0
40-44	33.60512745326713	34.0	34.0	34.0	34.0	34.0
45-49	33.62498550531472	34.0	34.0	34.0	34.0	34.0
50-54	33.48718136683216	34.0	34.0	34.0	34.0	34.0
55-59	33.440061310852684	34.0	34.0	34.0	34.0	34.0
60-64	33.416864483022884	34.0	34.0	34.0	34.0	34.0
65-69	33.2182466428976	34.0	34.0	34.0	34.0	34.0
70-74	33.500233914231444	34.0	34.0	34.0	34.0	34.0
75-79	33.48941388157675	34.0	34.0	34.0	34.0	34.0
80-84	33.589380249931295	34.0	34.0	34.0	34.0	34.0
85-89	33.55040871617441	34.0	34.0	34.0	34.0	34.0
90-94	33.40998333888704	34.0	34.0	34.0	34.0	34.0
95-99	33.46607397534156	34.0	34.0	34.0	34.0	34.0
100-104	33.17037581670672	34.0	34.0	34.0	34.0	34.0
105-109	33.269144079600665	34.0	34.0	34.0	34.0	34.0
110-114	33.16880885577378	34.0	34.0	34.0	34.0	34.0
115-119	33.29881438730015	34.0	34.0	34.0	34.0	34.0
120-124	33.18362534905265	34.0	34.0	34.0	34.0	34.0
125-129	33.10404467873828	34.0	34.0	34.0	34.0	34.0
130-134	33.305646822038135	34.0	34.0	34.0	34.0	34.0
135-139	33.114923791561424	34.0	34.0	34.0	34.0	34.0
140-144	33.159816407146124	34.0	34.0	34.0	34.0	34.0
145-149	33.21526578161655	34.0	34.0	34.0	34.0	34.0
150-154	33.20215132591788	34.0	34.0	34.0	34.0	34.0
155-159	33.218983385205966	34.0	34.0	34.0	34.0	34.0
160-164	33.31558111791639	34.0	34.0	34.0	34.0	34.0
165-169	33.32215372516611	34.0	34.0	34.0	34.0	34.0
170-174	32.45100268582511	34.0	34.0	34.0	28.4	34.0
175-179	33.21360906915833	34.0	34.0	34.0	34.0	34.0
180-184	32.90040320699514	34.0	34.0	34.0	34.0	34.0
185-189	32.6584828789244	34.0	34.0	34.0	31.2	34.0
190-194	33.10862802074031	34.0	34.0	34.0	34.0	34.0
195-199	32.86632272314405	34.0	34.0	34.0	34.0	34.0
200-204	32.97820387217168	34.0	34.0	34.0	34.0	34.0
205-209	32.41262021393582	34.0	34.0	34.0	31.2	34.0
210-214	32.660136625678966	34.0	34.0	34.0	31.2	34.0
215-219	32.935375387383786	34.0	34.0	34.0	34.0	34.0
220-224	33.034048452131024	34.0	34.0	34.0	34.0	34.0
225-229	33.22895531340598	34.0	34.0	34.0	34.0	34.0
230-234	32.65933819854043	34.0	34.0	34.0	34.0	34.0
235-239	32.586616015195446	34.0	34.0	34.0	31.2	34.0
240-244	32.974225065813584	34.0	34.0	34.0	34.0	34.0
245-249	32.745485687627045	34.0	34.0	34.0	34.0	34.0
250-254	32.85840247925622	34.0	34.0	34.0	34.0	34.0
255-259	32.52448265520344	34.0	34.0	34.0	31.2	34.0
260-264	32.79518204423282	34.0	34.0	34.0	34.0	34.0
265-269	32.57669201495571	34.0	34.0	34.0	34.0	34.0
270-274	32.56502683604993	34.0	34.0	34.0	34.0	34.0
275-279	32.09811184942782	34.0	34.0	34.0	22.8	34.0
280-284	31.983129976195762	34.0	34.0	34.0	25.6	34.0
285-289	32.29468605227534	34.0	34.0	34.0	25.6	34.0
290-294	32.06591832304467	34.0	34.0	34.0	25.6	34.0
295-299	31.523035051546707	34.0	34.0	34.0	20.0	34.0
300-301	31.427538442926085	34.0	34.0	34.0	20.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.2977921658151388
1101	2	0.23774649041197904
1101	3	0.17648225520124328
1101	4	-0.011883246752482535
1101	5	0.3256549773884956
1101	6	0.04759161881400331
1101	7	-0.07548365461111928
1101	8	0.14859607304616418
1101	9	0.07647449420630181
1101	10-14	0.031149815617595777
1101	15-19	-0.019846726260425385
1101	20-24	-0.0522473851472256
1101	25-29	0.027651385225894387
1101	30-34	0.005765169003772996
1101	35-39	0.08412705592179748
1101	40-44	0.04966042246145719
1101	45-49	0.037399584445772405
1101	50-54	0.0672166460273047
1101	55-59	0.016862697546756067
1101	60-64	0.02664636378268881
1101	65-69	-0.0632696301877047
1101	70-74	0.033680169098275314
1101	75-79	-0.04730182130693805
1101	80-84	-0.006121567094318436
1101	85-89	0.028891677346884137
1101	90-94	-0.14939184570950204
1101	95-99	-0.0709642917288349
1101	100-104	-0.09885777626536196
1101	105-109	-0.07993335442233729
1101	110-114	-0.1713966430296523
1101	115-119	-0.0890088707103942
1101	120-124	-0.05996948119128831
1101	125-129	-0.01629088896481079
1101	130-134	0.040534585021170244
1101	135-139	0.00804833382228054
1101	140-144	0.11073653588579191
1101	145-149	-0.10635713309188333
1101	150-154	-0.13539322541517151
1101	155-159	-0.020959323182971445
1101	160-164	-0.042078708354395644
1101	165-169	-0.02716306752485309
1101	170-174	-0.13280341890588687
1101	175-179	0.056537495293142115
1101	180-184	-0.15612824628270516
1101	185-189	-0.2706709825096496
1101	190-194	-0.05472498614879129
1101	195-199	-0.186356291642106
1101	200-204	0.16909232829382148
1101	205-209	0.007097553026774506
1101	210-214	-0.07422692395467578
1101	215-219	-0.12388338711427593
1101	220-224	-0.14445235523970723
1101	225-229	-0.045081643334192734
1101	230-234	-0.39186679862464757
1101	235-239	-0.16299835054027767
1101	240-244	-0.23322592527684805
1101	245-249	-0.2174368611244475
1101	250-254	-0.1526407248246784
1101	255-259	-0.36284511879231474
1101	260-264	-0.25628382504311276
1101	265-269	-0.4241809732905395
1101	270-274	-0.23385890470751036
1101	275-279	-0.5527942498265332
1101	280-284	-0.5877299107539891
1101	285-289	-0.49223448799196845
1101	290-294	-0.36202733530950937
1101	295-299	-0.8269504417394238
1101	300-301	-0.9569531612958606
1102	1	0.2170197944227965
1102	2	-0.14657002507190242
1102	3	-0.1203814431446446
1102	4	-0.116537304972681
1102	5	-0.17891607851949232
1102	6	-0.2584655737315984
1102	7	-0.10791779622887532
1102	8	0.1518015432937858
1102	9	-0.18520017625476015
1102	10-14	-0.08756641528694331
1102	15-19	-0.04895049891313619
1102	20-24	-0.014120075410261279
1102	25-29	-0.09491852445967908
1102	30-34	-0.07568539521432172
1102	35-39	-0.11826894415725064
1102	40-44	-0.059322403964891635
1102	45-49	-0.028010190064613028
1102	50-54	-0.05811921690030886
1102	55-59	-0.13832949233091085
1102	60-64	-0.14391785431575954
1102	65-69	-0.14355262854743245
1102	70-74	-0.07012726553739412
1102	75-79	-0.12558723068619315
1102	80-84	-0.19632176076637364
1102	85-89	-0.12100812700480645
1102	90-94	-0.005908515340536269
1102	95-99	-0.09422233010752024
1102	100-104	-0.18008383899139346
1102	105-109	-0.06765612669956766
1102	110-114	-0.27438433102000914
1102	115-119	-0.01541348721995206
1102	120-124	-0.0662823999083102
1102	125-129	-0.2010619409202974
1102	130-134	-0.36323451045125665
1102	135-139	-0.2997566502689466
1102	140-144	-0.30514062069214987
1102	145-149	-0.23304474796115215
1102	150-154	-0.20908188769457325
1102	155-159	-0.2549294028256668
1102	160-164	-0.2140436892836206
1102	165-169	-0.07082623623693252
1102	170-174	-0.3915382265183851
1102	175-179	-0.23733574831864246
1102	180-184	-0.3917449336999468
1102	185-189	-0.2617517911893259
1102	190-194	-0.2544625407425727
1102	195-199	-0.2725024152365023
1102	200-204	-0.11654155245952325
1102	205-209	-0.2415987177979133
1102	210-214	-0.21527789132686337
1102	215-219	-0.21516142509771896
1102	220-224	-0.2987259861956133
1102	225-229	-0.24457098148957357
1102	230-234	-0.421392697322311
1102	235-239	-0.3355071942390211
1102	240-244	-0.3235490414207973
1102	245-249	-0.44715089839644406
1102	250-254	-0.41467665296839584
1102	255-259	-0.5415506779709922
1102	260-264	-0.4770283794985488
1102	265-269	-0.688616616854901
1102	270-274	-0.5392133601530489
1102	275-279	-0.6829566260641542
1102	280-284	-1.0318170394668584
1102	285-289	-0.8273869632395012
1102	290-294	-0.7389580283788142
1102	295-299	-1.0971516361445772
1102	300-301	-1.0708841283918211
1103	1	-0.41747938011613783
1103	2	-0.13446849140054695
1103	3	-0.26020003778248224
1103	4	0.13434795790039544
1103	5	0.05608739194625656
1103	6	-0.016583914652898102
1103	7	-0.2144826625669296
1103	8	-0.5441464428557197
1103	9	-0.28482411109627037
1103	10-14	-0.2783259571070289
1103	15-19	-0.07332218775548682
1103	20-24	-0.08584617135197448
1103	25-29	-0.1617247940423283
1103	30-34	-0.0849878119917733
1103	35-39	-0.0812945628608972
1103	40-44	-0.01713644190080288
1103	45-49	-0.11786505793201485
1103	50-54	-0.10725630617951509
1103	55-59	-0.13529587390959819
1103	60-64	-0.17953698932668516
1103	65-69	-0.1272739874014306
1103	70-74	-0.017778524904713322
1103	75-79	-0.13016824547391082
1103	80-84	-0.09120939218330193
1103	85-89	0.013763586934700811
1103	90-94	0.0013249548507161535
1103	95-99	-0.12783604570186924
1103	100-104	-0.012228586823823662
1103	105-109	-0.18749895577619213
1103	110-114	-0.2790649501354139
1103	115-119	-0.04514106546228902
1103	120-124	-0.13391295412404247
1103	125-129	-0.09689934273749401
1103	130-134	-0.13964553908162003
1103	135-139	-0.06540884293665528
1103	140-144	-0.11921541805224223
1103	145-149	-0.13136598368224384
1103	150-154	-0.17877629063720946
1103	155-159	-0.10697305618691644
1103	160-164	-0.024486457970233744
1103	165-169	-0.20265374036423367
1103	170-174	-0.2015143450083201
1103	175-179	-0.13279355569014228
1103	180-184	-0.19101241053409979
1103	185-189	-0.4122594203318286
1103	190-194	-0.09231140097887902
1103	195-199	-0.3204246454323041
1103	200-204	0.05495200266989997
1103	205-209	-0.20744698197682254
1103	210-214	-0.18608049045081287
1103	215-219	-0.26429537723274876
1103	220-224	-0.5383794886210183
1103	225-229	-0.33124243230111006
1103	230-234	-0.4189935663112436
1103	235-239	-0.48435997982698353
1103	240-244	-0.465344036891544
1103	245-249	-0.4039069344579076
1103	250-254	-0.17330870124904152
1103	255-259	-0.5539423974789273
1103	260-264	-0.3382134482608308
1103	265-269	-0.46548891547965354
1103	270-274	-0.3081579574025639
1103	275-279	-0.555102795022897
1103	280-284	-0.5389384540051694
1103	285-289	-0.5417806764025599
1103	290-294	-0.6037320951764542
1103	295-299	-0.9194628345728688
1103	300-301	-0.5496756071056907
1104	1	0.2581543926961345
1104	2	0.034032625049540854
1104	3	-0.01684153500910668
1104	4	-0.11480582816725615
1104	5	0.15068977002438544
1104	6	0.041897555431766875
1104	7	0.0049648721692676645
1104	8	-8.810433401720275E-4
1104	9	-0.2160421068513969
1104	10-14	0.10417299745384412
1104	15-19	-0.0013060288171828915
1104	20-24	0.0907809658806471
1104	25-29	-0.06625055918541278
1104	30-34	-0.06905768677577129
1104	35-39	0.06675618630471547
1104	40-44	0.07807268537450085
1104	45-49	0.020134484441804545
1104	50-54	-0.04149463473932968
1104	55-59	-0.14652033955213284
1104	60-64	0.0327865681645747
1104	65-69	-0.09216143503084595
1104	70-74	-0.09475992792346233
1104	75-79	-0.0482791961528477
1104	80-84	-0.026519197218775048
1104	85-89	-0.03571606053534282
1104	90-94	-0.07610066116936309
1104	95-99	-0.026569683593315574
1104	100-104	-0.21906852023131762
1104	105-109	-0.11143122710117837
1104	110-114	-0.23965860175564302
1104	115-119	-0.11446117497697372
1104	120-124	-0.16606356047873305
1104	125-129	-0.10544353916958471
1104	130-134	-0.053249660873305515
1104	135-139	-0.0644778103584045
1104	140-144	-0.07635241397305492
1104	145-149	-0.017649033366247124
1104	150-154	-0.10267542351450487
1104	155-159	-0.0852831531563254
1104	160-164	-0.1331837224702639
1104	165-169	-0.19549978071424334
1104	170-174	-0.41823663692647983
1104	175-179	-0.003107818635839976
1104	180-184	-0.3320859384374586
1104	185-189	-0.40205672349339494
1104	190-194	-0.24382117287590432
1104	195-199	-0.12326113875332823
1104	200-204	0.05397284044142481
1104	205-209	-0.1511242450566499
1104	210-214	-0.17245269833934174
1104	215-219	-0.15416053531102136
1104	220-224	-0.19637887319270675
1104	225-229	-0.156029991181029
1104	230-234	-0.29635842851052985
1104	235-239	-0.2213259182368077
1104	240-244	-0.2883279174037696
1104	245-249	-0.4730629297554074
1104	250-254	-0.1687853031379305
1104	255-259	-0.3986485304587788
1104	260-264	-0.4480934397395089
1104	265-269	-0.4292109943649365
1104	270-274	-0.6994575368598781
1104	275-279	-0.5772795176304228
1104	280-284	-0.5882539872580246
1104	285-289	-0.6809227062113834
1104	290-294	-0.7340182693426733
1104	295-299	-1.1821331622490021
1104	300-301	-0.4239729092657498
1201	1	-0.2696914304303988
1201	2	0.06218673526633722
1201	3	-0.2035801908773962
1201	4	-0.08238569118780248
1201	5	0.18811748919983984
1201	6	-0.11874501484935962
1201	7	0.09892577803339009
1201	8	-0.046457540198979075
1201	9	0.08670414787706449
1201	10-14	0.06114611815240778
1201	15-19	0.0604630391559553
1201	20-24	-0.07084645667264056
1201	25-29	-0.04909813204947966
1201	30-34	0.00848568171895181
1201	35-39	-0.006852772351479075
1201	40-44	-0.030116086309753598
1201	45-49	-0.01836640709113624
1201	50-54	-0.11268228992438623
1201	55-59	-0.09261842483933691
1201	60-64	-0.11848407457234345
1201	65-69	-0.009483836670597157
1201	70-74	-0.162571422865895
1201	75-79	-0.10450242933453069
1201	80-84	-0.05159710219969327
1201	85-89	-0.029145379470833177
1201	90-94	-0.0252432897750694
1201	95-99	-0.049596229611417186
1201	100-104	-0.30475400074257664
1201	105-109	-0.044834549936084045
1201	110-114	-0.20704349402180355
1201	115-119	0.15499507325246498
1201	120-124	-0.050189274954902885
1201	125-129	-0.16790750290835632
1201	130-134	-0.03219948506634296
1201	135-139	-0.15410769920841005
1201	140-144	-0.0543840425620985
1201	145-149	-0.2132146985164738
1201	150-154	0.015688488565366754
1201	155-159	-0.039028424698926756
1201	160-164	0.06292519452551204
1201	165-169	-0.06519765936577926
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2404	270-274	0.6928366398469521
2404	275-279	0.858860040602508
2404	280-284	0.8647329605331393
2404	285-289	0.6217630367605054
2404	290-294	0.8239586382878485
2404	295-299	1.1282483638554268
2404	300-301	0.8584050747132821
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	15.0
16	49.0
17	62.0
18	84.0
19	208.0
20	401.0
21	557.0
22	594.0
23	566.0
24	544.0
25	556.0
26	663.0
27	802.0
28	1080.0
29	1672.0
30	2468.0
31	4374.0
32	12776.0
33	117728.0
34	4856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.24057845456665888	0.08196994435373696	0.08796774516010795	99.5894838559195
2	0.05398308529993935	0.1386232313874986	0.2379254500256586	99.56946823328691
3	0.11395821532104895	0.1872646696211389	99.55283062876946	0.14594648628836093
4	0.08330278897737496	99.558162007264	0.18926393655659593	0.16927126720202593
5	0.17993402419112992	0.23991203225483987	0.16194062177201693	99.418213321782
6	99.4948518876412	0.16993768951384494	0.05464662956915797	0.28056379327579883
7	99.8180667088734	0.06864149811735697	0.04265102795641598	0.07064076505281397
8	99.59948019059678	0.12861950618106693	0.09063343440738396	0.18126686881476792
9	30.195594948518877	18.676485288727466	27.747825797207692	23.380093965545967
10-14	59.719969344573656	11.399286928126354	8.67135383692646	20.20938989037353
15-19	36.44117157042418	22.249441871313852	19.958681816667223	21.350704741594747
20-24	59.501649395221754	39.83072873279798	0.5491319849388557	0.11848988704141815
25-29	16.154476691879644	16.28309619806071	59.38329279264269	8.179134317416947
30-34	6.286361667388624	56.503015560960975	0.5219419546166406	36.688680817033756
35-39	59.980673752957244	1.9406217720169272	19.722368464896206	18.35633601012962
40-44	38.252773982872945	57.68938056046117	0.28562860284562325	3.772216853820266
45-49	20.252840625104128	19.95375029155976	21.578754456699208	38.2146546266369
50-54	40.80783712638699	20.45903168838093	16.694412048915396	22.038719136316683
55-59	20.69028023058212	38.90919996001466	0.5865849188630835	39.81393489054014
60-64	39.97334310752724	0.26123754623304785	0.5039485521975275	59.26147079404218
65-69	37.71137249675119	22.745126786844825	25.203292126220383	14.3402085901836
70-74	40.11195894838559	42.95238412582053	16.777448268968044	0.15820865682583055
75-79	39.62133884242444	19.84139148978708	5.964079837392956	34.57318983039552
80-84	40.04795587739396	0.24177865923470523	39.53880787393389	20.171457589437445
85-89	36.849253719407024	3.5016141200180204	43.89286828194185	15.756263878633103
90-94	17.0919026991003	61.509496834388536	3.9252249250249913	17.47337554148617
95-99	19.295834721759412	0.8371876041319561	59.38180606464512	20.485171609463514
100-104	5.259075023392327	25.21053094584976	24.841318714128434	44.68907531662948
105-109	44.64768175729262	53.339908963072055	1.4901798745743056	0.5222294050610133
110-114	27.25709601530167	55.02949036648028	14.745316529933556	2.9680970882844937
115-119	33.73244740051583	24.082799618791196	23.86233830282108	18.322414677871894
120-124	26.203440209531554	22.959966410972417	34.417690221194405	16.41890315830162
125-129	47.13207019040447	27.199248245573116	21.090577078154467	4.578104485867949
130-134	40.26537997587455	49.06877086818306	4.952782091183547	5.713067064758846
135-139	23.79569340682044	7.0750221594279195	8.825250418196722	60.30403401555492
140-144	39.43892695813745	19.33005527557145	19.790973833741425	21.440043932549674
145-149	4.4203188313073145	37.885609938153124	21.690259117082533	36.00381211345703
150-154	34.218611501729455	6.145541063800009	23.847327837277653	35.78851959719288
155-159	34.94571700867062	47.125767259591996	0.13689044099515485	17.791625290742232
160-164	35.913013922304344	4.700260585016695	1.5271215019293956	57.85960399074956
165-169	25.248222223703237	35.71094390424334	34.3475044486061	4.69332942344732
170-174	16.454844148833367	38.35611508394037	21.042873233053644	24.146167534172626
175-179	83.20526235112999	2.347264523782548	5.743133818070338	8.704339307017134
180-184	25.994788299666105	25.18224289722554	14.53851126646984	34.28445753663852
185-189	25.250322566885973	14.7467716652982	46.0051024216509	13.99780334616492
190-194	35.35888994041827	23.39482558682004	10.77936099596124	30.46692347680045
195-199	5.317457249605786	40.755398032862054	35.32077624505649	18.606368472475665
200-204	63.5870862854828	0.7789382188109489	20.405176646072043	15.228798849634206
205-209	23.590389549801728	51.00536505714952	16.242060715118797	9.162184677929956
210-214	65.69309207237829	6.054317038221867	27.30727448432137	0.9453164050784766
215-219	40.50131627178513	19.800326568696057	16.350694791562532	23.34766236795628
220-224	47.84404678596421	7.65357059548802	42.13509280549169	2.367289813056083
225-229	15.817121530207604	2.629344529974341	62.61148322170016	18.942050718117898
230-234	20.22713186044187	43.832450264920524	20.906128161551536	15.034289713086075
235-239	57.15152121030358	33.611783131727144	4.405878236529041	4.830817421440234
240-244	25.18444466660002	49.77573394648272	16.920790429537806	8.119030957379453
245-249	40.67726348762038	19.41884101436236	22.041387583724884	17.862507914292376
250-254	36.01946082841814	7.77953280682462	25.581925422373285	30.619080942383953
255-259	4.947848978639741	39.46762637875304	6.915125462361292	48.669399180245925
260-264	38.49499544798371	20.671885601099415	38.2360037162581	2.597115234658772
265-269	21.152336330251995	43.50299580786841	5.622121658457909	29.722546203421686
270-274	19.097529081973374	19.870649034199893	46.05542203685116	14.97639984697557
275-279	21.89948080857899	35.32980985197379	19.177824727907705	23.592884611539514
280-284	29.40372756913545	51.50707683612505	9.137769696905067	9.951425897834428
285-289	27.708890423339525	22.71220963557982	33.606024170263595	15.97287577081706
290-294	41.173906436209776	18.459879332730797	20.298815455861536	20.06739877519789
295-299	53.87220070629154	35.129869090676316	7.946846760340984	3.0510834426911626
300-301	74.19281369738218	24.655019961951716	1.0175236462045496	0.1346426944615632
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.25
19	0.0
20	0.25
21	0.25
22	0.0
23	0.0
24	0.0
25	0.5
26	1.25
27	2.0
28	2.4166666666666665
29	1.9166666666666665
30	1.25
31	2.333333333333333
32	1.75
33	1.0
34	2.5
35	0.75
36	1.75
37	2.5
38	1.75
39	3.9166666666666665
40	3.4166666666666665
41	4.0
42	4.5
43	5.5
44	10.833333333333334
45	12.666666666666668
46	23.249999999999996
47	33.41666666666667
48	55.41666666666667
49	143.0
50	1518.8333333333333
51	1519.7499999999984
52	3907.4166666666792
53	5243.9166666666615
54	4815.083333333333
55	25291.583333333332
56	5349.666666666666
57	116.41666666666664
58	45.333333333333336
59	20.5
60	8.5
61	4.583333333333333
62	4.75
63	3.0
64	1.5833333333333333
65	0.5833333333333333
66	0.5833333333333333
67	0.0
68	0.3333333333333333
69	0.0
70	0.25
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.005331378494551998
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.6295444699923356
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
80-84	1.0
85-89	4.0
90-94	0.0
95-99	0.0
100-104	1.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	1.0
145-149	1.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	1.0
190-194	0.0
195-199	1.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	1.0
260-264	1.0
265-269	0.0
270-274	2.0
275-279	1.0
280-284	2.0
285-289	3.0
290-294	14.0
295-299	427.0
300-302	149594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.544333744293759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.856577210734706	2.8109693112525407
2	12.289191963631032	1.1169237946086434
3	5.9539521924035785	0.8117023757955416
4	3.3729285819035044	0.6131085268734797
5	2.5370288898665496	0.5764552997234347
6	2.170406217920516	0.5917830128952717
7	1.5251503152954977	0.4851554430042318
8	0.9972136676932102	0.36253373762953583
9	0.8945593195483209	0.36586584918863085
>10	6.701862443173486	6.2337143047549235
>50	0.7479102507699076	2.28516210722735
>100	0.4252822994573985	3.3154510012995235
>500	0.19064378941193721	6.774182799640132
>1k	0.23463851004546118	18.140015327713172
>5k	0.029329813755682648	12.568724800906333
>10k+	0.07332453438920664	42.94825230748726
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	16428	10.947985738562526	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	13882	9.251274532671355	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	12521	8.344273766285696	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	10908	7.269334577321648	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	10707	7.13538369264603	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	9555	6.367665189430542	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	9305	6.201059611475792	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2915	1.942621038952384	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	2904	1.935290393522375	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	2464	1.6420645763220152	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	2133	1.4214787911099263	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	1985	1.3228482889607145	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	1888	1.2582053247142713	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	1707	1.1375828862750326	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	1651	1.1002632368131684	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1444	0.9623138182666355	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1315	0.8763453400419846	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1278	0.8516877145046816	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1275	0.8496884475692246	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1103	0.7350638099363567	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	1076	0.7170704075172437	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1074	0.7157375628936057	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1008	0.6717536903135517	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	966	0.6437639532171537	No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	942	0.6277698177334977	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	893	0.5951151244543668	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	862	0.5744560327879777	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	851	0.5671253873579687	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	829	0.5524640964979508	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	771	0.5138116024124488	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	763	0.5084802239178967	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	752	0.5011495784878878	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	699	0.4658291959614808	No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	685	0.45649928359601477	No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	635	0.4231781680050648	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	517	0.3445403352104228	No Hit
CCTACGGGCGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	412	0.27456599246942787	No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	362	0.2412448768784779	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	285	0.18993035886841492	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	254	0.16927126720202593	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	246	0.16393988870747392	No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	244	0.16260704408383592	No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	220	0.14661290860017992	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	198	0.13195161774016195	No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	185	0.12328812768651494	No Hit
CCTACGGGCGGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCA	177	0.11795674919196295	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	176	0.11729032688014396	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	171	0.11395821532104895	No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	166	0.11062610376195395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	2.665689247275999E-4	0.0	0.0	0.0
160-164	0.0	6.664223118189997E-4	0.0	0.0	0.0
165-169	0.0	6.664223118189997E-4	0.0	0.0	0.0
170-174	0.0	6.664223118189997E-4	0.0	0.0	0.0
175-179	0.0	6.664223118189997E-4	0.0	0.0	0.0
180-184	0.0	6.664223118189997E-4	0.0	0.0	0.0
185-189	0.0	6.664223118189997E-4	0.0	0.0	0.0
190-194	0.0	6.664223118189997E-4	0.0	0.0	0.0
195-199	0.0	6.664223118189997E-4	0.0	0.0	0.0
200-204	0.0	6.664223118189997E-4	0.0	0.0	0.0
205-209	0.0	6.664223118189997E-4	0.0	0.0	0.0
210-214	0.0	6.664223118189997E-4	0.0	0.0	0.0
215-219	0.0	6.664223118189997E-4	0.0	0.0	0.0
220-224	0.0	6.664223118189997E-4	0.0	0.0	0.0
225-229	0.0	6.664223118189997E-4	0.0	0.0	0.0
230-234	0.0	6.664223118189997E-4	0.0	0.0	0.0
235-239	0.0	6.664223118189997E-4	0.0	0.0	0.0
240-244	0.0	6.664223118189997E-4	0.0	0.0	0.0
245-249	0.0	6.664223118189997E-4	0.0	0.0	0.0
250-254	0.0	6.664223118189997E-4	0.0	0.0	0.0
255-259	0.0	6.664223118189997E-4	0.0	0.0	0.0
260-264	0.0	6.664223118189997E-4	0.0	0.0	0.0
265-269	0.0	6.664223118189997E-4	0.0	0.0	0.0
270-274	0.0	6.664223118189997E-4	0.0	0.0	0.0
275-279	0.0	6.664223118189997E-4	0.0	0.0	0.0
280-284	0.0	6.664223118189997E-4	0.0	0.0	0.0
285-289	0.0	6.664223118189997E-4	0.0	0.0	0.0
290	0.0	6.664223118189997E-4	0.0	0.0	0.0
>>END_MODULE
