FastQCFastQC Report
Mon 14 Oct 2024
JCMS2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJCMS2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127452
Sequences flagged as poor quality0
Sequence length39-301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG105208.254087813451338No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG105028.239964849511972No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG101207.94024417035433No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG89737.040297523773656No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG88756.963405831214889No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG88106.912406239211625No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG84206.60640868719204No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG82446.468317484229356No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG79216.2148887424285215No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA8490.6661331324734018No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7700.6041490129617424No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7680.6025797947462574No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7340.5759030850830117No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA7110.5578570756049337No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6770.5311803659416878No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6670.5233342748642626No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA6230.4888114741235916No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA5810.4558578915984057No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3500.27461318770988297No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3350.2628440510937451No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3240.25421335090857733No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3240.25421335090857733No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3020.23695195053824186No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3010.23616734143049933No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2910.2283212503530741No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2880.22596742302984654No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2850.22361359570661896No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2720.2134136773059662No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2710.21262906819822366No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2680.21027524087499608No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2680.21027524087499608No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2630.20635219533628343No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2620.20556758622854096No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2570.20164454068982832No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2570.20164454068982832No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2550.20007532247434326No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2540.19929071336660076No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2470.1937984496124031No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2450.19222923139691805No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2430.19066001318143302No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2410.18909079496594797No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2410.18909079496594797No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2410.18909079496594797No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2340.18359853121175032No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG2310.18124470388852273No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1880.14750651225559427No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1860.14593729404010922No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1660.13024511188525875No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1590.1247528481310611No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1580.12396823902331858No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1540.12082980259234849No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1500.1176913661613784No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1500.1176913661613784No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1410.11062988419169571No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1370.1074914477607256No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1360.1067068386529831No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1360.1067068386529831No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1350.10592222954524057No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1330.10435301132975551No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG1320.10356840222201298No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1310.10278379311427048No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA1290.10121457489878542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)