FastQCFastQC Report
Mon 14 Oct 2024
JCMS1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJCMS1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161387
Sequences flagged as poor quality0
Sequence length35-301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1886611.689913066108176No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT155819.65443313278021No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT145489.014356794537354No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT144518.954252820859177No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT136948.485193974731546No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT132648.21875367904478No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT123057.624529856803831No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT100076.200623346366188No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16571.026724581285977No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13550.8395967457106212No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12350.7652413143561748No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12270.7602842855992119No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12080.7485113423014245No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11270.6983214261371734No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10480.649370767162163No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8670.5372179915358734No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT5730.3550471847174803No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT5040.31229281168867384No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT4740.29370395385006226No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT4420.27387583882221No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT4010.24847106644277422No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT3810.23607849455036653No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT3510.21748963671175497No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT2850.1765941494668096No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2550.15800529162819807No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2450.1518090056819942No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2450.1518090056819942No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT2300.14251457676268844No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2070.1282631190864196No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC2060.1276434904917992No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2060.1276434904917992No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2050.12702386189717882No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1990.12330609032945652No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT1790.11091351843704883No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1680.1040976038962246No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC1640.10161908951774307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)