FastQCFastQC Report
Mon 14 Oct 2024
JCFS2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJCFS2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152204
Sequences flagged as poor quality0
Sequence length38-301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1544310.146251084071379No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1542710.13573887677065No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1534310.080549788441829No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG136918.995164384641665No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG131168.617381934771753No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG130568.577961157394023No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG127338.36574597251058No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG124788.198207668655225No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG117377.7113610680402624No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1980.13008856534650864No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCATGCTTTCGAGCCTCAG1740.11432025439541667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)