FastQCFastQC Report
Mon 14 Oct 2024
JCFS1_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJCFS1_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188244
Sequences flagged as poor quality0
Sequence length41-301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1940610.308960710567137No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1908310.13737489641104No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG1897010.077346422727949No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG168278.938930324472494No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG163878.705191134910011No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG162928.654724718981747No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG159148.453921506130342No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG154188.190433692441724No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG143277.610866747412932No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2320.12324429995112726No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCATGCTTTCGAGCCTCAG1910.10146405728735045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)