FastQCFastQC Report
Mon 14 Oct 2024
JAMS2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJAMS2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147745
Sequences flagged as poor quality0
Sequence length80-301
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1847012.501269078479812No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1746211.819012487732241No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1651411.177366408338692No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1650811.17330535720329No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1590710.766523401807168No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1503710.177670987173846No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT143809.732985887847304No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT107227.2570983789637555No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2350.1590578361365867No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2310.15635046871298522No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2270.15364310128938372No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2260.15296625943348338No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2180.14755152458628043No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT2100.14213678973907748No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2040.13807573860367525No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1930.13063047818877122No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1790.12115469220616602No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT1560.10558732952045756No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)