##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JAMS1_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	163443
Sequences flagged as poor quality	0
Sequence length	86-301
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.683608352759066	40.0	40.0	40.0	40.0	40.0
2	39.0554627607178	40.0	40.0	40.0	40.0	40.0
3	39.19771418782083	40.0	40.0	40.0	40.0	40.0
4	38.90797403376101	40.0	40.0	40.0	40.0	40.0
5	39.10500908573631	40.0	40.0	40.0	40.0	40.0
6	39.29582790330574	40.0	40.0	40.0	40.0	40.0
7	39.33794656241014	40.0	40.0	40.0	40.0	40.0
8	39.38899799930251	40.0	40.0	40.0	40.0	40.0
9	38.89084267909914	40.0	40.0	40.0	40.0	40.0
10-14	39.542852248184374	40.0	40.0	40.0	40.0	40.0
15-19	39.593115642762314	40.0	40.0	40.0	40.0	40.0
20-24	39.59836273196161	40.0	40.0	40.0	40.0	40.0
25-29	39.64037370826527	40.0	40.0	40.0	40.0	40.0
30-34	39.328803313693456	40.0	40.0	40.0	40.0	40.0
35-39	39.56741861076951	40.0	40.0	40.0	40.0	40.0
40-44	39.34711917916338	40.0	40.0	40.0	40.0	40.0
45-49	39.56830944121192	40.0	40.0	40.0	40.0	40.0
50-54	39.52269109108374	40.0	40.0	40.0	40.0	40.0
55-59	39.40451900662616	40.0	40.0	40.0	40.0	40.0
60-64	39.42625624835569	40.0	40.0	40.0	40.0	40.0
65-69	39.508912587262834	40.0	40.0	40.0	40.0	40.0
70-74	39.331730328004255	40.0	40.0	40.0	40.0	40.0
75-79	39.18447899267635	40.0	40.0	40.0	36.8	40.0
80-84	39.64583616306602	40.0	40.0	40.0	40.0	40.0
85-89	39.579707594951444	40.0	40.0	40.0	40.0	40.0
90-94	39.279162026896394	40.0	40.0	40.0	40.0	40.0
95-99	39.30406137957196	40.0	40.0	40.0	40.0	40.0
100-104	39.16585944861174	40.0	40.0	40.0	40.0	40.0
105-109	39.273234541917006	40.0	40.0	40.0	40.0	40.0
110-114	39.072620256727156	40.0	40.0	40.0	40.0	40.0
115-119	38.990580144638464	40.0	40.0	40.0	40.0	40.0
120-124	39.08822456896024	40.0	40.0	40.0	40.0	40.0
125-129	38.956380023249906	40.0	40.0	40.0	40.0	40.0
130-134	39.04747930210429	40.0	40.0	40.0	40.0	40.0
135-139	39.18525005764013	40.0	40.0	40.0	40.0	40.0
140-144	39.2400599626763	40.0	40.0	40.0	40.0	40.0
145-149	39.06892893199131	40.0	40.0	40.0	40.0	40.0
150-154	39.23351165622212	40.0	40.0	40.0	40.0	40.0
155-159	38.757320026595934	40.0	40.0	40.0	36.8	40.0
160-164	39.289436038009455	40.0	40.0	40.0	40.0	40.0
165-169	39.21728904199274	40.0	40.0	40.0	40.0	40.0
170-174	39.14137291734228	40.0	40.0	40.0	40.0	40.0
175-179	39.26178923473228	40.0	40.0	40.0	40.0	40.0
180-184	39.14793707513171	40.0	40.0	40.0	40.0	40.0
185-189	39.18938770015848	40.0	40.0	40.0	40.0	40.0
190-194	39.25640476525549	40.0	40.0	40.0	40.0	40.0
195-199	39.16662118421617	40.0	40.0	40.0	40.0	40.0
200-204	39.41808080375444	40.0	40.0	40.0	40.0	40.0
205-209	38.61569205729565	40.0	40.0	40.0	36.8	40.0
210-214	39.08115986367502	40.0	40.0	40.0	40.0	40.0
215-219	39.07505093830499	40.0	40.0	40.0	40.0	40.0
220-224	39.20884031988644	40.0	40.0	40.0	40.0	40.0
225-229	39.28445295625731	40.0	40.0	40.0	40.0	40.0
230-234	38.90784847613395	40.0	40.0	40.0	40.0	40.0
235-239	39.26343394540883	40.0	40.0	40.0	40.0	40.0
240-244	39.22671675854938	40.0	40.0	40.0	40.0	40.0
245-249	39.165328911541735	40.0	40.0	40.0	40.0	40.0
250-254	39.117891735451224	40.0	40.0	40.0	40.0	40.0
255-259	39.194033028825274	40.0	40.0	40.0	40.0	40.0
260-264	39.276826589489275	40.0	40.0	40.0	40.0	40.0
265-269	39.302951056396196	40.0	40.0	40.0	40.0	40.0
270-274	39.04781531269694	40.0	40.0	40.0	36.8	40.0
275-279	39.122571328923776	40.0	40.0	40.0	40.0	40.0
280-284	37.5659897327957	40.0	36.8	40.0	36.8	40.0
285-289	39.119247642764925	40.0	40.0	40.0	40.0	40.0
290-294	39.27013760990742	40.0	40.0	40.0	40.0	40.0
295-299	39.10370242192009	40.0	40.0	40.0	40.0	40.0
300-301	39.17870504557976	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.004424564677520948
1101	2	-0.2370951634919649
1101	3	-0.33082811964080605
1101	4	-0.03494846788876771
1101	5	-0.028663684229165654
1101	6	-0.12744482808297874
1101	7	-0.013575930578298312
1101	8	-0.03187091296626221
1101	9	-0.35288602624029863
1101	10-14	-0.05083346588841664
1101	15-19	-0.03871712355044821
1101	20-24	-0.007151822105974759
1101	25-29	-0.042161670605715074
1101	30-34	-0.11721794065947932
1101	35-39	-0.09424071156504965
1101	40-44	0.09691390168458724
1101	45-49	-0.11545729972102237
1101	50-54	-0.10413702282012594
1101	55-59	-0.07362466955750335
1101	60-64	-0.05434455736175181
1101	65-69	-0.1010671114532471
1101	70-74	-0.01780416744412605
1101	75-79	-0.2725298394082287
1101	80-84	-0.036190669343817206
1101	85-89	-0.1911504681359375
1101	90-94	-0.1971612929794304
1101	95-99	-0.1895140557005348
1101	100-104	-0.026946085500959782
1101	105-109	-0.2271872672273787
1101	110-114	-0.1718053949118854
1101	115-119	-0.2875258440144677
1101	120-124	-0.17209938153375504
1101	125-129	-0.3077598067998011
1101	130-134	-0.34006989582655933
1101	135-139	-0.18257654163468118
1101	140-144	-0.2689582284694083
1101	145-149	-0.18971625778007706
1101	150-154	-0.2433475654582935
1101	155-159	-0.05089581343386129
1101	160-164	-0.19341457773842308
1101	165-169	-0.11376458969935754
1101	170-174	-0.04409592880965363
1101	175-179	-0.08312204961904257
1101	180-184	-0.2973114255174494
1101	185-189	-0.11522242906575997
1101	190-194	-0.08673115365857598
1101	195-199	-0.03709594280687867
1101	200-204	-0.08658656951052279
1101	205-209	-0.21104394115261016
1101	210-214	-0.08953153326398677
1101	215-219	-0.2552446344147796
1101	220-224	-0.09714673818507435
1101	225-229	-0.14373099532920008
1101	230-234	-0.1641490642153869
1101	235-239	-0.1816281580917689
1101	240-244	-0.2496399525558317
1101	245-249	-0.2048438974400213
1101	250-254	-0.11650700063002972
1101	255-259	-0.13816082399507223
1101	260-264	-0.24054995152182101
1101	265-269	-0.16846669134722703
1101	270-274	-0.1222083613599807
1101	275-279	-0.19337291676491475
1101	280-284	-0.23627781019267502
1101	285-289	-0.18358823915938416
1101	290-294	-0.20171958915984334
1101	295-299	-0.20231281274397617
1101	300-301	-0.34716419441431157
1102	1	0.017208898730430633
1102	2	-0.1318100262290045
1102	3	-0.2811533061952005
1102	4	-0.08152887454311752
1102	5	0.11039293246145832
1102	6	-0.010596741549122157
1102	7	-0.022284084203270993
1102	8	-0.19598400942686567
1102	9	0.34817510883192426
1102	10-14	-0.002883852689265609
1102	15-19	-0.0499144946738852
1102	20-24	-0.02425047343753306
1102	25-29	-0.1436801206639089
1102	30-34	-0.16312195928068007
1102	35-39	-0.1366970681064288
1102	40-44	-0.17520494544350385
1102	45-49	-0.0038888736239428567
1102	50-54	-0.13189357789645584
1102	55-59	-0.2808130039177428
1102	60-64	-0.09848777636633343
1102	65-69	-0.17030514801106023
1102	70-74	-0.17020507112044214
1102	75-79	-0.18121197810884837
1102	80-84	-0.06953084870987425
1102	85-89	-0.036753261317286956
1102	90-94	-0.27589066930585915
1102	95-99	-0.27266323075360077
1102	100-104	-0.2270638372638203
1102	105-109	-0.006745013513402398
1102	110-114	-0.401985445572528
1102	115-119	-0.1418230987553386
1102	120-124	-0.16373900637115923
1102	125-129	-0.16511039213560963
1102	130-134	-0.2387349194453776
1102	135-139	-0.27624943819112957
1102	140-144	-0.3257118397516763
1102	145-149	-0.20810744008993254
1102	150-154	-0.21264995472371595
1102	155-159	-0.19707778098366902
1102	160-164	-0.2573127087714866
1102	165-169	-0.16734164179767674
1102	170-174	-0.2724686268311416
1102	175-179	-0.07875975756727627
1102	180-184	-0.3157683297414522
1102	185-189	-0.3540038352408743
1102	190-194	-0.24233600064973615
1102	195-199	-0.17498599552133243
1102	200-204	-0.13966523042652312
1102	205-209	-0.24661592718670988
1102	210-214	-0.18473794115061537
1102	215-219	-0.1774627490173586
1102	220-224	-0.12393937185385084
1102	225-229	-0.1393988258214307
1102	230-234	-0.07759056486576554
1102	235-239	-0.22633822860591124
1102	240-244	-0.23679953357862615
1102	245-249	-0.3726949277679594
1102	250-254	-0.24433064682705918
1102	255-259	-0.2510464267882426
1102	260-264	-0.277940243847425
1102	265-269	-0.22639234343184
1102	270-274	-0.294555865981053
1102	275-279	-0.34351736805540156
1102	280-284	-0.2812891061466729
1102	285-289	-0.31149951457528147
1102	290-294	-0.38343657416703536
1102	295-299	-0.3230384820327501
1102	300-301	-0.49079630235868166
1103	1	0.19454608722003996
1103	2	0.2992652870024415
1103	3	0.16075774984224722
1103	4	-0.2123838931304718
1103	5	0.5015811466980011
1103	6	0.09057357374758368
1103	7	0.12377126482605405
1103	8	-0.05020402870264462
1103	9	-0.10062015483322284
1103	10-14	0.022174663075574585
1103	15-19	0.08221272548308178
1103	20-24	0.03950279656109501
1103	25-29	-0.05045142728651797
1103	30-34	0.05831270958893242
1103	35-39	-0.024313755425723116
1103	40-44	0.06116570029517732
1103	45-49	0.0584104517237094
1103	50-54	-0.022660478626193026
1103	55-59	-0.05809511874632278
1103	60-64	-0.10250815362091714
1103	65-69	0.04732393088195863
1103	70-74	0.025895713679219057
1103	75-79	-0.08231620501234005
1103	80-84	-0.0027540347211783
1103	85-89	-0.11731501265970934
1103	90-94	-0.1784653629586188
1103	95-99	0.08204419042222355
1103	100-104	-0.030274631701374233
1103	105-109	0.048493868491284786
1103	110-114	-0.14669699727293306
1103	115-119	-0.16700828394053246
1103	120-124	-0.2703450920107002
1103	125-129	-0.21576714554328902
1103	130-134	-0.26987907475309925
1103	135-139	-0.10924957587527473
1103	140-144	-0.11866241114090315
1103	145-149	-0.08003914875026652
1103	150-154	-0.06764100525379746
1103	155-159	-0.21597906144629064
1103	160-164	-0.15236434032868829
1103	165-169	-0.24981444917754914
1103	170-174	-0.046259071550949216
1103	175-179	-0.1963805754111405
1103	180-184	-0.2339288694633339
1103	185-189	-0.34054933985329683
1103	190-194	-0.29208403865056454
1103	195-199	-0.18613290451211384
1103	200-204	-0.15868216557728942
1103	205-209	-0.22893728199877472
1103	210-214	-0.1368073339635032
1103	215-219	-0.24110587169393938
1103	220-224	-0.17633645294981903
1103	225-229	-0.0935114514582196
1103	230-234	-0.14886138959383288
1103	235-239	-0.17201907674029115
1103	240-244	-0.364135345226714
1103	245-249	-0.22402357984010024
1103	250-254	-0.20549270107461126
1103	255-259	-0.18365278779609895
1103	260-264	-0.275814400531992
1103	265-269	-0.16296400803324929
1103	270-274	-0.2560428548286282
1103	275-279	-0.22020193366189744
1103	280-284	-0.2588025303515451
1103	285-289	-0.11224300899907291
1103	290-294	-0.23514248081717426
1103	295-299	-0.20803323234370197
1103	300-301	-0.1989573044719961
1104	1	0.15965904118057495
1104	2	0.00893071451173455
1104	3	0.3370803120384167
1104	4	0.2107788177645773
1104	5	0.14999407206259718
1104	6	-0.040796171748553434
1104	7	0.05805779613860551
1104	8	-0.07233728578014365
1104	9	0.020254880911700468
1104	10-14	0.09483694503153117
1104	15-19	0.06324789848851253
1104	20-24	-0.06772625691331058
1104	25-29	-0.022198639182420266
1104	30-34	-0.12904788520660304
1104	35-39	-0.037038948448319786
1104	40-44	0.03493750469895218
1104	45-49	0.011438761703693956
1104	50-54	0.01671041070752466
1104	55-59	-0.13500103810577002
1104	60-64	-0.04623706411562978
1104	65-69	0.11978032207441203
1104	70-74	0.02329920238382499
1104	75-79	-0.13648263337950794
1104	80-84	0.009044649865614929
1104	85-89	0.05128092671689899
1104	90-94	-0.06483653825172553
1104	95-99	-0.06280568089604088
1104	100-104	-0.06643705663703514
1104	105-109	0.04630341953503603
1104	110-114	0.12696042337334035
1104	115-119	-0.037777514709766535
1104	120-124	0.05175956912741242
1104	125-129	0.09078704376184277
1104	130-134	0.15442747371702126
1104	135-139	0.10232606038436387
1104	140-144	0.22260724856741376
1104	145-149	0.15229142030892717
1104	150-154	0.0922503301765758
1104	155-159	-0.04647949038538002
1104	160-164	0.006960795502024553
1104	165-169	0.15938200492597332
1104	170-174	0.052545618183103215
1104	175-179	-0.07414437295189913
1104	180-184	0.04787839390527182
1104	185-189	-0.04710924834628116
1104	190-194	-0.13242147073521693
1104	195-199	-0.11954440007974654
1104	200-204	0.06358263282133692
1104	205-209	-0.1252484058191854
1104	210-214	0.06039026397758818
1104	215-219	-0.015924287478902954
1104	220-224	0.09395236603788248
1104	225-229	0.07382054739794341
1104	230-234	0.0065689792937817515
1104	235-239	0.07719453492685346
1104	240-244	-0.16500466178376172
1104	245-249	-0.19913367420669204
1104	250-254	0.029800407304001908
1104	255-259	0.030833915092095765
1104	260-264	0.004054058146877537
1104	265-269	-0.023999181038675488
1104	270-274	-0.06994048136566988
1104	275-279	-0.044599989138013996
1104	280-284	-0.047101071958636
1104	285-289	0.10234663927086984
1104	290-294	-7.015314319929189E-4
1104	295-299	0.009914473582945504
1104	300-301	0.038001474376436306
1201	1	0.03604854762788534
1201	2	0.07656077231520442
1201	3	-0.41148942265848376
1201	4	0.039473052239159756
1201	5	0.26419297527884567
1201	6	-0.3275310168649028
1201	7	-0.18978568985517086
1201	8	-0.019662022700252635
1201	9	-0.14362536215929822
1201	10-14	-0.1293664046053422
1201	15-19	0.057959622217580886
1201	20-24	-0.09796488148719362
1201	25-29	-0.06767070083946436
1201	30-34	-0.07663940736460262
1201	35-39	-0.07339584781098552
1201	40-44	-0.07258880328978279
1201	45-49	-0.12348639021568175
1201	50-54	-0.19999034518398418
1201	55-59	-0.2535811477841463
1201	60-64	-0.10294078428755427
1201	65-69	-0.11368343959743754
1201	70-74	-0.02112350400419416
1201	75-79	-0.06741193529147438
1201	80-84	-0.07334159587323086
1201	85-89	-0.11284831590945998
1201	90-94	-0.21586069713715972
1201	95-99	-0.24310210893813888
1201	100-104	-0.13754125109382898
1201	105-109	-0.23556111284973014
1201	110-114	-0.1427861306506628
1201	115-119	-0.2417674361563229
1201	120-124	-0.1317886745364305
1201	125-129	-0.26252850388897997
1201	130-134	-0.289473519316914
1201	135-139	-0.2026806092465776
1201	140-144	-0.17306786629845305
1201	145-149	-0.3085741495739853
1201	150-154	-0.2451055316879618
1201	155-159	-0.13228651572997308
1201	160-164	-0.1778265173777811
1201	165-169	-0.1979560712560371
1201	170-174	-0.1355557602072892
1201	175-179	-0.10195821614146894
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2404	200-204	0.20571824099900482
2404	205-209	0.20930079259234446
2404	210-214	0.3559631423420555
2404	215-219	0.2083116295677172
2404	220-224	0.2676249649494835
2404	225-229	0.2457693634002922
2404	230-234	0.22542763497843765
2404	235-239	0.21114070348104264
2404	240-244	0.3025758647642576
2404	245-249	0.2947507205572393
2404	250-254	0.29487762454183297
2404	255-259	0.2372730973247812
2404	260-264	0.3234392618533164
2404	265-269	0.1913089525103686
2404	270-274	0.24487052025221345
2404	275-279	0.2758353690028841
2404	280-284	0.24267095333919997
2404	285-289	0.2231085160174544
2404	290-294	0.22220183671038285
2404	295-299	0.30227422553004857
2404	300-301	0.2771506078007633
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	12.0
18	38.0
19	37.0
20	51.0
21	100.0
22	164.0
23	265.0
24	398.0
25	322.0
26	305.0
27	370.0
28	426.0
29	473.0
30	529.0
31	579.0
32	726.0
33	845.0
34	1113.0
35	1519.0
36	2163.0
37	3216.0
38	6669.0
39	129159.0
40	13962.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.06240707769681173	0.006730175045734599	0.11135380530215427	99.8195089419553
2	0.012236756769985684	0.021414324347474943	0.1664198920718053	99.79992902681073
3	0.011624847806268853	0.12603782358375704	99.59802499954112	0.2643123290688497
4	0.08626860740441622	99.38265939807762	0.09728162111561829	0.4337903734023482
5	0.19578691042137017	0.13276799862949162	0.07770293007348128	99.59374216087565
6	99.7791278916809	0.06240707769681173	0.017131354661869887	0.14133367596042656
7	99.88803436060279	0.06974908683761312	0.028144368373071958	0.014072184186535979
8	99.73262850045582	0.120531316728156	0.07831476416854806	0.06852541864747955
9	29.230985725910564	21.698084347448347	26.312537092442014	22.75839283419908
10-14	59.8131458673666	10.485979821711544	9.51891485104899	20.18195945987286
15-19	20.780822672124227	39.24242702348831	19.878367381900723	20.09838292248674
20-24	59.66153337860906	39.856953188573385	0.34544153007470496	0.13607190274285225
25-29	0.36905832614428274	0.6797476796191945	59.48140942102139	39.46978457321513
30-34	0.48053449826545036	59.55911235109488	0.31558402623544596	39.64476912440423
35-39	40.50965780119063	19.829665387933407	19.75942683381974	19.90124997705622
40-44	39.80776172733002	59.297859192501356	0.3724845970766567	0.5218944830919647
45-49	20.25709268674706	19.88693305923166	39.44910458080187	20.40686967321941
50-54	59.334079770929314	20.201538150914995	19.70754330255808	0.7568387755976089
55-59	20.199702648629795	39.419002343324586	0.4099288436947437	39.971366164350876
60-64	39.99596189497256	0.16299260292579065	0.34348366097049127	59.497561841131166
65-69	39.984704147623326	20.147819117368133	39.63314427659796	0.2343324584105774
70-74	40.11943001535704	59.60891564643332	0.20569862276145198	0.06595571544819906
75-79	59.387798804476176	19.81559320374687	19.94101919323556	0.8555887985413876
80-84	39.889502762430936	0.07464375959814737	39.70754330255808	20.328310175412835
85-89	20.313455994283	0.6732647097693133	40.049583217084425	38.96369607886326
90-94	20.0854125622545	59.302137761407714	0.7918405305857735	19.82060914575201
95-99	19.889991556637828	0.36734743823497024	59.638159102311526	20.104501902815677
100-104	39.875185080946146	20.036465535174557	39.11112198822824	0.9772273956510567
105-109	40.66151907098543	39.607567210386556	19.51505732920547	0.21585638942254745
110-114	39.95509110265415	59.60915798876666	0.23127470295272942	0.20447620562646074
115-119	20.961809082120876	58.69201306885623	19.945668799941263	0.40050904908163143
120-124	58.67402503640435	20.48751238971623	20.473073016727646	0.3653895571517725
125-129	0.8693002646813728	39.74013991524812	39.20331273884649	20.18724708122402
130-134	39.126199691870546	0.8691991693476051	20.669637011756155	39.33496412702569
135-139	0.3120495167515523	19.838150317311527	78.51838889446363	1.3314112714732897
140-144	40.06277725089485	19.991801021812954	19.850949918928016	20.09447180836418
145-149	19.46155964144767	39.55749992351699	39.92877902530058	1.052161409734757
150-154	0.9105805272091153	20.11182482001931	58.4306099947257	20.54698465804587
155-159	20.569184587276315	78.80854511123158	0.19726641612689663	0.425003885365206
160-164	20.341057191632046	19.894023850752298	19.47011925376148	40.29479970385418
165-169	39.42337226875845	20.331756744353953	20.154681123151384	20.09018986373621
170-174	0.6221509731816708	39.31494863338493	20.01101368756616	40.05188670586723
175-179	39.89475809659004	0.5080981197187838	19.891453990320194	39.705689793370986
180-184	39.85718918455881	39.374422546242194	0.16691855378044826	20.60146971541855
185-189	40.067061119847274	0.3802169696450533	0.6042843244632357	58.948437586044435
190-194	20.003304106269848	39.94909228858309	39.52800230063696	0.5196013045101051
195-199	20.522783036473662	39.431448972973634	20.08272503105248	19.963042959500225
200-204	79.08904566397239	0.38939504261685215	19.998653882630805	0.5229054107799527
205-209	20.55043962969535	39.802365495340595	0.502101778710542	39.14509309625351
210-214	39.657719065305045	0.69153720484847	58.81688520678199	0.8338585230644975
215-219	39.89231061046423	19.924372678712377	1.0945157954635845	39.08880091535981
220-224	0.6884778471912038	20.187477437237277	78.53175307312476	0.5922916424467519
225-229	59.99877625693709	0.2609020210116684	19.694553731498534	20.045767990552704
230-234	20.198613499109726	59.304424443043935	19.91556172865945	0.5814003291868839
235-239	59.1438693531906	20.10719989231061	0.6201929842810203	20.128737770217764
240-244	40.179523107328386	20.229941321520133	19.35692302044263	20.233612550708855
245-249	59.21264371332595	0.35635397991837636	20.258209786273273	20.1727925204824
250-254	0.8306767911009405	39.37013944552202	39.799183763377044	20.0
255-259	19.925841170387866	39.42214852569555	20.32343529152619	20.3285750123904
260-264	39.878359939547096	20.22370023189931	39.46167542662742	0.4362644019261716
265-269	20.6165217550923	59.26685553101271	19.544889954905067	0.5717327589899225
270-274	19.579766632197902	0.38217495854570377	39.51894660197145	40.51911180728494
275-279	39.78339747786555	0.7544375982818647	19.914827482821707	39.547337441030884
280-284	0.8541726579087454	39.91898820923559	0.36749004179082556	58.859349091064836
285-289	59.10813605575373	39.70385417877662	0.7659407830731859	0.422068982396456
290-294	20.211854658306045	20.021562379155224	39.42036115149468	20.34622181104405
295-299	20.278590203311104	39.633239351784404	20.041575511093537	20.046594933810955
300-301	0.9689821070743697	98.38708188021928	0.5983803100865867	0.04555570261976071
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.25
45	0.25
46	1.3333333333333333
47	0.5
48	10.833333333333334
49	273.5000000000001
50	32139.74999999978
51	24277.499999999924
52	728.083333333334
53	211.75000000000009
54	387.8333333333333
55	562.25
56	90.83333333333333
57	10.5
58	3.75
59	1.5
60	0.25
61	0.5
62	0.5
63	0.25
64	0.25
65	0.25
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.25
81	0.25
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.118340950667817E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	1.2237430629065123E-4
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
85-89	1.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	3.0
130-134	3.0
135-139	1.0
140-144	0.0
145-149	0.0
150-154	1.0
155-159	1.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	2.0
295-299	867.0
300-302	162564.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.001309324963443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.15622133904005	1.1838989739542225
2	10.180372974625497	0.40748150731447663
3	4.830327117089575	0.2900093610616545
4	2.2928767960868237	0.1835502285200345
5	2.720880464689697	0.27226617230471784
6	1.8954448180984407	0.2276022833648428
7	1.5897279119535308	0.22270761060430855
8	1.3145826964231122	0.2104709287029729
9	0.7948639559767654	0.14316917824562692
>10	12.656679914399266	5.927448713006981
>50	1.8954448180984407	2.474868914545132
>100	0.42800366860287375	1.6329851997332403
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.24457352491592785	86.82354092864179
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	21965	13.43893589814186	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	19483	11.920363674186108	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	19046	11.652992174641923	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	18735	11.462711771076155	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	18031	11.031980568149141	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	16559	10.131360780210837	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	15891	9.722655604706228	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	12197	7.462540457529536	No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	252	0.15418219195682897	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	251	0.1535703578617622	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	238	0.14561651462589403	No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	222	0.13582716910482553	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	215	0.13154433043935806	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	210	0.12848515996402415	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	203	0.12420232129855668	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	199	0.12175498491828955	No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	178	0.10890646892188714	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	174	0.10645913254162002	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
