FastQCFastQC Report
Mon 14 Oct 2024
JAFS2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameJAFS2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84149
Sequences flagged as poor quality0
Sequence length76-301
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG68538.143887627898133No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG65667.802825939702195No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG64697.687554219301478No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG60157.148035033096056No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG58166.911549751036851No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG57096.784394348120595No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG55276.568111326337806No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG53966.412435085384259No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG49365.865785689669515No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG9481.1265731024729944No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG8571.0184315915815993No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG8350.9922874900474159No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG7670.911478448941758No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG7400.8793925061498058No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG7360.8746390331435906No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG7230.8591902458733912No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG6970.8282926713329926No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG6630.7878881507801638No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5890.6999489001651832No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5830.6928186906558604No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5690.6761815351341074No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5590.6642978526185694No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5180.615574754304864No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5160.6131980178017564No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG5030.5977492305315572No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG4730.5620981829849434No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCATGCTCTCATGCCTCAG4430.5264471354383295No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3050.3624523167239064No Hit
GACTACTCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2920.3470035294537071No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2920.3470035294537071No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2760.32798963742884646No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2750.32680126917729263No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2710.3220477961710775No Hit
GACTACAGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2660.3161059549133085No Hit
GACTACCGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2610.3101641136555396No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2550.30303390414621684No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2470.2935269581337865No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2460.2923385898822327No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2460.2923385898822327No Hit
GACTACACGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2390.28402001212135614No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2350.279266539115141No Hit
GACTACCCGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2260.26857122485115686No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2240.2661944883480493No Hit
GACTACCAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2200.2614410153418341No Hit
GACTACAAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG2180.2590642788387265No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2150.2554991740840652No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2080.24718059632318862No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1780.2115295487765749No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1770.2103411805250211No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1730.20558770751880592No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1690.20083423451259078No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1600.19013892024860665No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1560.18538544724239148No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG1400.1663715552175308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)