##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JAFS2_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84149
Sequences flagged as poor quality	0
Sequence length	76-301
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.65727459625189	40.0	40.0	40.0	40.0	40.0
2	39.095627993202534	40.0	40.0	40.0	40.0	40.0
3	39.30480457284103	40.0	40.0	40.0	40.0	40.0
4	39.011943100928114	40.0	40.0	40.0	40.0	40.0
5	39.29791203698202	40.0	40.0	40.0	40.0	40.0
6	39.326337805559184	40.0	40.0	40.0	40.0	40.0
7	39.44916754803979	40.0	40.0	40.0	40.0	40.0
8	39.48282213692379	40.0	40.0	40.0	40.0	40.0
9	38.95380812606211	40.0	40.0	40.0	40.0	40.0
10-14	39.60086037861413	40.0	40.0	40.0	40.0	40.0
15-19	39.49486743752154	40.0	40.0	40.0	40.0	40.0
20-24	39.465709634101415	40.0	40.0	40.0	40.0	40.0
25-29	39.61783978419233	40.0	40.0	40.0	40.0	40.0
30-34	39.47256414217638	40.0	40.0	40.0	40.0	40.0
35-39	39.61563417271744	40.0	40.0	40.0	40.0	40.0
40-44	39.54511164719723	40.0	40.0	40.0	40.0	40.0
45-49	39.473676454859834	40.0	40.0	40.0	40.0	40.0
50-54	39.525831560684026	40.0	40.0	40.0	40.0	40.0
55-59	39.38780971847556	40.0	40.0	40.0	40.0	40.0
60-64	39.383484058039905	40.0	40.0	40.0	40.0	40.0
65-69	39.490190020083425	40.0	40.0	40.0	40.0	40.0
70-74	39.387220287822785	40.0	40.0	40.0	40.0	40.0
75-79	39.3714724937238	40.0	40.0	40.0	40.0	40.0
80-84	39.45636525570876	40.0	40.0	40.0	40.0	40.0
85-89	39.57213923253354	40.0	40.0	40.0	40.0	40.0
90-94	39.43082737147339	40.0	40.0	40.0	40.0	40.0
95-99	39.26814821857248	40.0	40.0	40.0	36.8	40.0
100-104	39.227590140945495	40.0	40.0	40.0	40.0	40.0
105-109	39.353865899745685	40.0	40.0	40.0	40.0	40.0
110-114	39.348056948636895	40.0	40.0	40.0	40.0	40.0
115-119	39.287856820288546	40.0	40.0	40.0	40.0	40.0
120-124	39.18703703090174	40.0	40.0	40.0	40.0	40.0
125-129	39.34131796303999	40.0	40.0	40.0	40.0	40.0
130-134	39.340129538296985	40.0	40.0	40.0	40.0	40.0
135-139	39.14012240774853	40.0	40.0	40.0	40.0	40.0
140-144	39.41122364448277	40.0	40.0	40.0	40.0	40.0
145-149	39.02891030745279	40.0	40.0	40.0	36.8	40.0
150-154	39.25846724351051	40.0	40.0	40.0	40.0	40.0
155-159	39.086840353713036	40.0	40.0	40.0	40.0	40.0
160-164	39.320737852999905	40.0	40.0	40.0	40.0	40.0
165-169	39.269287819720454	40.0	40.0	40.0	40.0	40.0
170-174	38.98921745744984	40.0	40.0	40.0	40.0	40.0
175-179	39.280060853855666	40.0	40.0	40.0	40.0	40.0
180-184	39.32236379195588	40.0	40.0	40.0	40.0	40.0
185-189	39.21491390019229	40.0	40.0	40.0	40.0	40.0
190-194	39.33725560111726	40.0	40.0	40.0	40.0	40.0
195-199	39.243678584320165	40.0	40.0	40.0	40.0	40.0
200-204	39.44741483823425	40.0	40.0	40.0	40.0	40.0
205-209	39.227888843986975	40.0	40.0	40.0	40.0	40.0
210-214	39.3247723869066	40.0	40.0	40.0	40.0	40.0
215-219	39.32060760215846	40.0	40.0	40.0	40.0	40.0
220-224	39.24032614638553	40.0	40.0	40.0	40.0	40.0
225-229	39.34393229847624	40.0	40.0	40.0	40.0	40.0
230-234	39.1527325457009	40.0	40.0	40.0	40.0	40.0
235-239	39.33493712411153	40.0	40.0	40.0	40.0	40.0
240-244	39.25545439418071	40.0	40.0	40.0	40.0	40.0
245-249	39.130710533197046	40.0	40.0	40.0	40.0	40.0
250-254	39.24430075831411	40.0	40.0	40.0	40.0	40.0
255-259	39.11901490479473	40.0	40.0	40.0	40.0	40.0
260-264	39.09245727054461	40.0	40.0	40.0	40.0	40.0
265-269	39.15994960420282	40.0	40.0	40.0	40.0	40.0
270-274	37.86005657641381	40.0	36.8	40.0	33.6	40.0
275-279	39.12461733247409	40.0	40.0	40.0	40.0	40.0
280-284	38.23054449502574	40.0	40.0	40.0	36.8	40.0
285-289	39.048221268705504	40.0	40.0	40.0	40.0	40.0
290-294	39.11465227977	40.0	40.0	40.0	40.0	40.0
295-299	39.030167468086034	40.0	40.0	40.0	40.0	40.0
300-301	38.18311457839241	40.0	40.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.15630262593807487
1101	2	-0.2743501821460015
1101	3	-0.09464117480201395
1101	4	-0.3337846296723015
1101	5	0.12692831164624607
1101	6	-0.3169510693031299
1101	7	0.11074259864702185
1101	8	-0.27475539065802224
1101	9	-0.1301803873140983
1101	10-14	-0.14107549889074278
1101	15-19	-0.33458386336888424
1101	20-24	-0.20446451614832029
1101	25-29	-0.18134364536774683
1101	30-34	-0.14905558825397947
1101	35-39	-0.10315421182172457
1101	40-44	-0.2019337706900899
1101	45-49	-0.2622696407652896
1101	50-54	-0.407435864361851
1101	55-59	-0.34999363889446045
1101	60-64	-0.30792814917035116
1101	65-69	-0.24067046468807973
1101	70-74	-0.12877621779629322
1101	75-79	-0.25774052608191766
1101	80-84	-0.037160507733283055
1101	85-89	-0.16359015611906358
1101	90-94	-0.27709086518959936
1101	95-99	-0.2726505230185765
1101	100-104	-0.4882023455642468
1101	105-109	-0.31005894511470444
1101	110-114	-0.2695102940117806
1101	115-119	-0.3927350568485082
1101	120-124	-0.24782308018099997
1101	125-129	-0.41227695064878134
1101	130-134	-0.44753326797529525
1101	135-139	-0.4142174702156822
1101	140-144	-0.4359402774747352
1101	145-149	-0.4187387024818392
1101	150-154	-0.35113364575374817
1101	155-159	-0.49854003054623064
1101	160-164	-0.46623917029778283
1101	165-169	-0.420720128819454
1101	170-174	-0.2703133600143133
1101	175-179	-0.23395515783436593
1101	180-184	-0.436451331077194
1101	185-189	-0.35481139489518654
1101	190-194	-0.41271838447480036
1101	195-199	-0.3606739557752974
1101	200-204	-0.36405091930866007
1101	205-209	-0.3742603173014416
1101	210-214	-0.3269463339453935
1101	215-219	-0.25901754016122425
1101	220-224	-0.37299149306724644
1101	225-229	-0.2199852767345405
1101	230-234	-0.27066783637751257
1101	235-239	-0.5040120141764604
1101	240-244	-0.32619997311608984
1101	245-249	-0.4916682550632743
1101	250-254	-0.3456915954597406
1101	255-259	-0.4630124389979642
1101	260-264	-0.48089982291057964
1101	265-269	-0.29094542871374074
1101	270-274	0.08859749501283432
1101	275-279	-0.3167329893331754
1101	280-284	-0.46600418448538505
1101	285-289	-0.47248762598165683
1101	290-294	-0.5203977774581361
1101	295-299	-0.766458000331788
1101	300-301	-0.8262608982743203
1102	1	0.1136441773755692
1102	2	0.20305678972592034
1102	3	0.13087009129966276
1102	4	-0.3242689301197075
1102	5	0.20451370288477477
1102	6	-0.32414191044231444
1102	7	0.011638750465685632
1102	8	-0.137264345417897
1102	9	-0.01918191469201247
1102	10-14	-0.12841313050881809
1102	15-19	-0.09400967740724298
1102	20-24	-0.20194501842586732
1102	25-29	-0.2124643082227209
1102	30-34	-0.25843206663414264
1102	35-39	-0.2578708359110422
1102	40-44	-0.1443854149867505
1102	45-49	-0.31814193575197436
1102	50-54	-0.2809527533769014
1102	55-59	-0.3664514738024778
1102	60-64	-0.41540148764000406
1102	65-69	-0.2674766228595544
1102	70-74	-0.3196633134804401
1102	75-79	-0.09882834355349246
1102	80-84	-0.22339788659058257
1102	85-89	-0.1797344054736456
1102	90-94	-0.14498484083265595
1102	95-99	-0.04547400949519442
1102	100-104	-0.2679571541013388
1102	105-109	-0.08331496338917788
1102	110-114	-0.2833404982478882
1102	115-119	-0.241035342049166
1102	120-124	-0.27539698229364973
1102	125-129	-0.4367690637017745
1102	130-134	-0.24618971607824136
1102	135-139	-0.17927701092098403
1102	140-144	-0.4372270595733312
1102	145-149	-0.4495998462581099
1102	150-154	-0.24345485468012384
1102	155-159	-0.34170400064746786
1102	160-164	-0.5341682891494202
1102	165-169	-0.19836634484205007
1102	170-174	-0.23158013751725548
1102	175-179	-0.3559074982368955
1102	180-184	-0.352150883677119
1102	185-189	-0.34629051847762327
1102	190-194	-0.422320590386974
1102	195-199	-0.3376524508349803
1102	200-204	-0.2664825590096669
1102	205-209	-0.41557791729062643
1102	210-214	-0.28720747501834154
1102	215-219	-0.39583816111468906
1102	220-224	-0.3031051678303669
1102	225-229	-0.35133437044525806
1102	230-234	-0.5406217447077992
1102	235-239	-0.583186959772064
1102	240-244	-0.42078386400994106
1102	245-249	-0.4085248302224258
1102	250-254	-0.3228647298284386
1102	255-259	-0.38010690664594193
1102	260-264	-0.5437575064324633
1102	265-269	-0.584472320026606
1102	270-274	-0.5236296382475132
1102	275-279	-0.4603335462178677
1102	280-284	-0.6313827173915882
1102	285-289	-0.7316174260708763
1102	290-294	-0.577567667838359
1102	295-299	-0.58212920778724
1102	300-301	-0.9536793615136858
1103	1	0.1595113536775301
1103	2	-0.13728402887576863
1103	3	-0.051483786706391754
1103	4	0.4143837215764279
1103	5	0.4274791432415199
1103	6	-0.03763131958389465
1103	7	-0.18504862013472945
1103	8	0.20291721215496494
1103	9	-0.00787438164534393
1103	10-14	0.11487401712615508
1103	15-19	0.1675606363369866
1103	20-24	0.06988170557477247
1103	25-29	0.08662790391296937
1103	30-34	-0.03836517700203501
1103	35-39	0.001215005783492984
1103	40-44	-0.07122089834397372
1103	45-49	0.14884325297234113
1103	50-54	0.07490008943246096
1103	55-59	0.07307711355219482
1103	60-64	-0.1693431981034479
1103	65-69	0.09739039418136741
1103	70-74	0.07020768508620279
1103	75-79	0.03667093977188074
1103	80-84	-0.05276562030614684
1103	85-89	-0.04264251186001644
1103	90-94	-0.04751709119099701
1103	95-99	0.058682703357163746
1103	100-104	-0.04212501522572154
1103	105-109	-0.07544746856516582
1103	110-114	0.1151305958753781
1103	115-119	0.041812245146246596
1103	120-124	-0.005863617108111896
1103	125-129	0.17788295095024154
1103	130-134	0.05615142104549875
1103	135-139	0.08414073075763184
1103	140-144	0.11884874867639184
1103	145-149	0.20639633147719394
1103	150-154	-0.02109460623291426
1103	155-159	-0.03824803632193152
1103	160-164	0.07592886007816446
1103	165-169	0.07260036960291671
1103	170-174	0.10952513402280317
1103	175-179	-0.05146411553263874
1103	180-184	-0.06567214502447172
1103	185-189	0.040172294072142734
1103	190-194	-0.0944339842394939
1103	195-199	-0.021137082145685326
1103	200-204	-0.07205669501858836
1103	205-209	-0.16193880278193973
1103	210-214	-0.059113829533394835
1103	215-219	0.006996685198423336
1103	220-224	-0.11756606924779334
1103	225-229	-0.06797714795759902
1103	230-234	-0.27879661331745353
1103	235-239	-0.3432761459482023
1103	240-244	-0.20211847143149697
1103	245-249	-0.3396922135637297
1103	250-254	-0.13707081359974183
1103	255-259	-0.018871041062247684
1103	260-264	-0.25517652219930653
1103	265-269	0.04553245780425641
1103	270-274	-0.2601163399516011
1103	275-279	-0.23704958380346852
1103	280-284	-0.182202911690041
1103	285-289	-0.327763309173271
1103	290-294	-0.15017477088024833
1103	295-299	-0.05131718093939952
1103	300-301	0.004093009397372782
1104	1	-0.09243111615219846
1104	2	0.22249995745806217
1104	3	-0.2857373823420346
1104	4	-0.023484796635948157
1104	5	0.1241783221691648
1104	6	0.16917972356373667
1104	7	0.4005578161908332
1104	8	-0.039547107016247196
1104	9	-0.1652006614712107
1104	10-14	0.01623512364513857
1104	15-19	-0.04677782181036605
1104	20-24	-0.0932526968057914
1104	25-29	0.0026250384255632753
1104	30-34	-0.093393556348488
1104	35-39	-0.08433081446227675
1104	40-44	-0.0363170574887377
1104	45-49	-0.10024475055441684
1104	50-54	-0.0504760057898892
1104	55-59	-0.181278978363828
1104	60-64	-0.11720234837622456
1104	65-69	-0.1780380512917077
1104	70-74	-0.051024474499392625
1104	75-79	-0.041512782055420416
1104	80-84	-0.19164256966416104
1104	85-89	0.025115244242925883
1104	90-94	-0.049941734382706215
1104	95-99	-0.22427315711563978
1104	100-104	-0.024733710877896442
1104	105-109	-0.02870696049220811
1104	110-114	-0.056952944103677794
1104	115-119	0.05115814253975515
1104	120-124	-0.2245884752113838
1104	125-129	-0.030735553485754963
1104	130-134	0.09745852414794598
1104	135-139	-0.2764037118499587
1104	140-144	-0.05341112898933176
1104	145-149	-0.15676011311420268
1104	150-154	-0.2628645957628635
1104	155-159	-0.2736919113426026
1104	160-164	-0.22069317111156295
1104	165-169	-0.3949039010754305
1104	170-174	-0.29729693023024595
1104	175-179	-0.3298792805736923
1104	180-184	-0.25111326476877593
1104	185-189	-0.2715596726441234
1104	190-194	-0.19033964357712563
1104	195-199	-0.2798354895959534
1104	200-204	-0.2855134647325883
1104	205-209	-0.16620397057827319
1104	210-214	-0.008295512456186316
1104	215-219	-0.1228649999285949
1104	220-224	-0.2312267294120005
1104	225-229	-0.2190985454337664
1104	230-234	-0.3208972360099125
1104	235-239	-0.02926281041064982
1104	240-244	-0.4566729432450316
1104	245-249	-0.2924006502199248
1104	250-254	-0.26100314401248426
1104	255-259	-0.3357608851136007
1104	260-264	-0.46629681756493113
1104	265-269	-0.42276533246410963
1104	270-274	-0.26823889464383655
1104	275-279	-0.36535729306670106
1104	280-284	-0.10785453454781901
1104	285-289	-0.21784982485079496
1104	290-294	-0.22218667367409495
1104	295-299	-0.2298620944487766
1104	300-301	-0.6327244543518802
1201	1	0.041453594236081415
1201	2	-0.1593934121403393
1201	3	0.10370504223680399
1201	4	0.35196967312619876
1201	5	-0.275366514221723
1201	6	0.24894437067316488
1201	7	-0.13226114525902943
1201	8	0.05481608023917062
1201	9	0.37877535917767347
1201	10-14	-0.018501630816295744
1201	15-19	0.05415748389859942
1201	20-24	-0.08245088725291083
1201	25-29	-0.12858419806524068
1201	30-34	0.0276244559187262
1201	35-39	0.19700470217617294
1201	40-44	0.0796941550782222
1201	45-49	-0.09613717652094067
1201	50-54	-0.030082455791280438
1201	55-59	-0.02714503814568303
1201	60-64	0.14312903293431134
1201	65-69	-0.05153587261223436
1201	70-74	0.13046368847138012
1201	75-79	0.049830783207816864
1201	80-84	0.06552486948545777
1201	85-89	-0.01983493754498511
1201	90-94	-0.005202482072192538
1201	95-99	-0.03969876284403284
1201	100-104	0.07453636211479164
1201	105-109	-0.007680622061407405
1201	110-114	0.09960113432878615
1201	115-119	0.06610083078044227
1201	120-124	0.13724439093166296
1201	125-129	-0.02698322909841977
1201	130-134	0.09571558057157858
1201	135-139	0.25661507752585777
1201	140-144	0.07746506095821104
1201	145-149	0.05192685088901783
1201	150-154	0.09008702922672995
1201	155-159	-0.010129978562495978
1201	160-164	0.010341215944031035
1201	165-169	0.044439997234363204
1201	170-174	0.24304888309716688
1201	175-179	0.09765256700200098
1201	180-184	0.1858882061867817
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2404	240-244	0.3883777302817677
2404	245-249	0.29532745132501503
2404	250-254	0.21404173421556294
2404	255-259	0.19634365642171758
2404	260-264	0.2758293503291185
2404	265-269	0.3727454312431817
2404	270-274	-0.045461957105850104
2404	275-279	0.2394118137505501
2404	280-284	0.23714949389155038
2404	285-289	0.34068127894159517
2404	290-294	0.40304973210990624
2404	295-299	0.3475984339128573
2404	300-301	0.38311061755523923
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	5.0
17	15.0
18	10.0
19	18.0
20	21.0
21	29.0
22	55.0
23	98.0
24	156.0
25	161.0
26	167.0
27	192.0
28	174.0
29	232.0
30	261.0
31	270.0
32	407.0
33	448.0
34	603.0
35	732.0
36	1017.0
37	1581.0
38	3348.0
39	66733.0
40	7416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.049911466565259244	0.005941841257768958	0.08793925061498056	99.85620744156199
2	0.008318676617388412	0.017825735608689452	0.1497361791129914	99.82411940866093
3	0.004753473006215166	0.13547398067713223	99.63398257852143	0.22578996779522037
4	0.07962067285410403	99.41769955673864	0.08556251411187299	0.4171172562953808
5	0.17587850122996113	0.10576477438828745	0.08080904110565783	99.6375476832761
6	99.7944122924812	0.07724393635099645	0.020202260276414457	0.10814151089139502
7	99.87522133358685	0.06536025383545853	0.042781257055936495	0.016637155521753082
8	99.76113798143768	0.0974461966274109	0.09031598711808815	0.05109983481681303
9	30.363997195450924	20.29851810479031	26.82622491057529	22.511259789183473
10-14	59.81366385815636	10.96103340503155	9.053702361287716	20.171600375524367
15-19	38.21388251791465	21.450759961496868	19.914437485888126	20.42092003470035
20-24	59.80962340610109	39.77207096935198	0.35104398150899	0.0672616430379446
25-29	16.838702777216604	16.94446755160489	60.58348881151291	5.633340859665593
30-34	1.9622336569656205	58.30657524153585	0.330366373931954	39.40082472756658
35-39	59.61354264459471	2.3002055877075187	19.75543381383023	18.330817953867545
40-44	38.15018597963137	45.16750050505651	0.16708457616846306	16.51522893914366
45-49	20.151635788898265	19.907307276378805	22.079882113869445	37.861174820853485
50-54	42.708528918941404	20.15947901935852	18.48269141641612	18.64930064528396
55-59	20.30089484129342	25.201012489750322	15.788660590143675	38.70943207881258
60-64	39.97076614101178	0.10814151089139502	0.5333396712973416	59.38775267679949
65-69	26.274346694553707	20.923124457806985	51.86989744381989	0.9326314038194154
70-74	40.471069174915925	57.6472685355738	1.748565045336249	0.13309724417402466
75-79	41.699426251717206	19.930979079816137	5.637896858407291	32.73169781005937
80-84	39.95336765397944	1.438187185374271	38.45552338985307	20.152921770793224
85-89	35.39710268934127	2.8704528979048574	55.79925606379312	5.933188348960748
90-94	19.420055617617	58.47526917500535	16.060418795902358	6.044256411475293
95-99	4.309176906804839	14.495757374087894	60.642217098851994	20.552848620255272
100-104	35.904737004729874	20.357949278634756	8.245668243291423	35.49164547334395
105-109	70.6136952439807	26.971929741164168	2.0728258027713737	0.34154921208375916
110-114	54.47127611532336	43.11957787654791	0.8059800822380149	1.6031659258907138
115-119	36.627528343593276	41.99320229125568	18.71271361680888	2.666555748342167
120-124	26.505311044929375	34.61817282941195	34.90719635297948	3.969319772679196
125-129	5.764335373462476	49.90694634262285	22.58054548695704	21.748172796957633
130-134	20.346306970111115	14.539663675797728	46.44601580604908	18.66801354804207
135-139	3.902786856022342	23.005050805157765	36.842593142789234	36.24956919603066
140-144	39.70745180251809	17.49061720824212	19.888428563618774	22.913502425621026
145-149	2.374766766100567	26.67863042677347	37.21165159312124	33.73495121400473
150-154	35.744508890367975	16.980602833507653	10.486355424550728	36.78853285157364
155-159	21.484976704383378	70.92707045735476	0.2208329371493772	7.367119901112485
160-164	23.64861652562518	17.76932585338024	1.9561186650185414	56.62593895597604
165-169	38.81002186935438	22.35523438242845	20.787534468004186	18.04720928021299
170-174	3.933868974042027	25.10435485404583	20.261005990301417	50.70077018161072
175-179	44.69121422458876	4.057953789103356	18.143957402301037	33.106874584006846
180-184	38.26780450698868	38.753446800418374	1.6580298564229343	21.32071883617001
185-189	39.23675408924186	1.3906156029447772	7.935541995402648	51.43708831241071
190-194	22.03720211564747	35.149224460688174	37.04284780412432	5.770725619540025
195-199	33.86288162064121	41.72243048638729	6.913921264259888	17.500766628711613
200-204	74.42960039936291	1.5667863170656333	21.4438871324316	2.559726151139848
205-209	22.038890341597927	39.242636746143056	3.6836475146789645	35.03482539758005
210-214	27.017614757410797	34.392754415575155	37.011671856799865	1.577958970214182
215-219	38.875127772363136	19.165141322176527	19.871871062828344	22.08785984263199
220-224	11.874866284736255	32.3255758670692	39.37908574417002	16.420472104024533
225-229	54.559868780754506	2.1009342239760382	24.395131575819526	18.944065419449927
230-234	20.217272446335606	56.02039603489671	21.892694986569044	1.8696365321986355
235-239	40.13240782561153	19.49414029999762	6.398839945800747	33.97461192859011
240-244	36.8195973090546	23.409560938502867	6.827441937861031	32.943399814581504
245-249	50.8816887346376	7.520146433071054	21.364489980269568	20.233674852021775
250-254	22.83357501129151	6.763496327287423	63.78895571350465	6.613972947916419
255-259	16.692181519956257	39.98906506287589	20.4255116837426	22.893241733425253
260-264	37.75001782870183	32.75132526683624	23.99648180283833	5.5021751016236005
265-269	22.8105165569211	56.93750445717546	17.118168635747736	3.133810350155704
270-274	6.973891586972181	4.063765097974892	23.160489601087793	65.80185371396514
275-279	49.888747842706564	11.435200372742269	17.38885481593473	21.28719696861643
280-284	3.190424684725375	36.324866580295485	8.004944552078257	52.47976418290088
285-289	51.45139243816338	25.573793873985238	18.935732708925155	4.039080978926224
290-294	26.02024927672664	20.068131518077674	22.052408494331484	31.859210710864193
295-299	24.407791466489083	24.004899395899727	32.29510536079532	19.29220377681587
300-301	10.975047179702244	53.061138903034475	35.93984962406015	0.02396429320312734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.25
41	0.0
42	0.0
43	0.0
44	0.0
45	0.3333333333333333
46	2.333333333333333
47	2.333333333333333
48	7.416666666666666
49	104.91666666666666
50	2870.333333333334
51	1544.2499999999995
52	101.16666666666664
53	223.25
54	8373.16666666666
55	13870.08333333338
56	1663.0833333333298
57	165.75
58	29.499999999999993
59	13.166666666666666
60	7.0
61	3.5833333333333335
62	1.0833333333333333
63	0.8333333333333333
64	2.0
65	0.5
66	0.5
67	0.0
68	0.0
69	0.25
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0011883682515537914
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	2.3771602443720732E-4
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
75-79	1.0
80-84	1.0
85-89	1.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	1.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	2.0
145-149	7.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	1.0
190-194	0.0
195-199	1.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	1.0
280-284	0.0
285-289	0.0
290-294	2.0
295-299	466.0
300-302	83665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.452815838572056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.674406191619966	2.6571914104742778
2	14.86522551374433	1.3238422322309238
3	6.111555911395784	0.8164089888174547
4	3.7630104083266613	0.6702396938763384
5	2.7755537763544167	0.6179514908079716
6	2.2151054176674676	0.5918073892737882
7	1.174272751534561	0.3660174214785678
8	1.0942087002935683	0.3897847865096436
9	0.6938884440886042	0.2780781708635872
>10	6.244995996797438	4.997088497783693
>50	0.1868161195623165	0.592995757525342
>100	0.5337603416066187	7.436808518223627
>500	0.4003202562049639	13.378649775992585
>1k	0.08006405124099279	6.677441205480754
>5k	0.1868161195623165	59.20569466066144
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9335	11.093417628254644	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7646	9.08626365138029	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7389	8.780853010730965	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6705	7.968009126668171	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6662	7.9169092918513595	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6170	7.332232112086894	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5914	7.028009839689123	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	3398	4.038075318779784	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1211	1.4391139526316414	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1010	1.2002519340693294	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	951	1.1301382072276556	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	928	1.1028057374419187	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	927	1.1016173691903648	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	870	1.0338803788517985	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	819	0.9732735980225553	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	764	0.9079133441870967	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	761	0.9043482394324353	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	727	0.8639437188796064	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	723	0.8591902458733912	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	703	0.8354228808423155	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	665	0.7902648872832714	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	664	0.7890765190317176	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	618	0.7344115794602432	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	618	0.7344115794602432	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	520	0.6179514908079716	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	487	0.5787353385066965	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	468	0.5561563417271744	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	446	0.530012240192991	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	438	0.5205052941805607	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	434	0.5157518211743455	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	367	0.4361311483202414	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	353	0.4194939927984884	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	334	0.3969149960189664	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	327	0.3885964182580898	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	298	0.3541337389630299	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	296	0.35175700245992225	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	286	0.33987331994438436	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	284	0.33749658344127675	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	271	0.3220477961710775	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	267	0.31729432316486234	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	244	0.2899618533791251	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	203	0.2412387550654197	No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	180	0.21390628527968247	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	141	0.1675599234690846	No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	134	0.15924134570820805	No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	99	0.11764845690382536	No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	92	0.10932987914294882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
