##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	JAFS1_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	132370
Sequences flagged as poor quality	0
Sequence length	38-301
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.56020246279368	40.0	40.0	40.0	40.0	40.0
2	39.24715569993201	40.0	40.0	40.0	40.0	40.0
3	39.13412404623404	40.0	40.0	40.0	40.0	40.0
4	38.76833119286847	40.0	40.0	40.0	40.0	40.0
5	39.300475938656795	40.0	40.0	40.0	40.0	40.0
6	39.23952557225957	40.0	40.0	40.0	40.0	40.0
7	39.23949535393216	40.0	40.0	40.0	40.0	40.0
8	39.397688297952705	40.0	40.0	40.0	40.0	40.0
9	39.01947571201934	40.0	40.0	40.0	40.0	40.0
10-14	39.532443907229734	40.0	40.0	40.0	40.0	40.0
15-19	39.45307244843998	40.0	40.0	40.0	40.0	40.0
20-24	39.430160912593486	40.0	40.0	40.0	40.0	40.0
25-29	39.58577321145275	40.0	40.0	40.0	40.0	40.0
30-34	39.41448968799577	40.0	40.0	40.0	40.0	40.0
35-39	39.57970467132934	40.0	40.0	40.0	40.0	40.0
40-44	39.496002840544236	40.0	40.0	40.0	40.0	40.0
45-49	39.425938097288636	40.0	40.0	40.0	40.0	40.0
50-54	39.52398330729978	40.0	40.0	40.0	40.0	40.0
55-59	39.43169346065514	40.0	40.0	40.0	40.0	40.0
60-64	39.415333010999646	40.0	40.0	40.0	40.0	40.0
65-69	39.46452919134534	40.0	40.0	40.0	40.0	40.0
70-74	39.31835488939926	40.0	40.0	40.0	40.0	40.0
75-79	39.27167291188202	40.0	40.0	40.0	40.0	40.0
80-84	39.44249365405536	40.0	40.0	40.0	40.0	40.0
85-89	39.5411673102304	40.0	40.0	40.0	40.0	40.0
90-94	39.36102263455321	40.0	40.0	40.0	40.0	40.0
95-99	39.241286150312774	40.0	40.0	40.0	36.8	40.0
100-104	39.20511317276602	40.0	40.0	40.0	40.0	40.0
105-109	39.28497174458312	40.0	40.0	40.0	40.0	40.0
110-114	39.241243842736694	40.0	40.0	40.0	40.0	40.0
115-119	39.164910562511054	40.0	40.0	40.0	40.0	40.0
120-124	39.0866260709267	40.0	40.0	40.0	40.0	40.0
125-129	39.18453936930539	40.0	40.0	40.0	40.0	40.0
130-134	39.22488782193728	40.0	40.0	40.0	40.0	40.0
135-139	39.21154418597623	40.0	40.0	40.0	40.0	40.0
140-144	39.32265244294014	40.0	40.0	40.0	40.0	40.0
145-149	38.96253248715624	40.0	40.0	40.0	36.8	40.0
150-154	39.18683892414627	40.0	40.0	40.0	40.0	40.0
155-159	39.05130250831067	40.0	40.0	40.0	40.0	40.0
160-164	39.28785131459655	40.0	40.0	40.0	40.0	40.0
165-169	39.21338773043216	40.0	40.0	40.0	40.0	40.0
170-174	38.98209731036567	40.0	40.0	40.0	40.0	40.0
175-179	39.24001208824418	40.0	40.0	40.0	40.0	40.0
180-184	39.23927470534905	40.0	40.0	40.0	40.0	40.0
185-189	39.16455401938514	40.0	40.0	40.0	40.0	40.0
190-194	39.26782160638869	40.0	40.0	40.0	40.0	40.0
195-199	39.18437582635106	40.0	40.0	40.0	40.0	40.0
200-204	39.40915238102433	40.0	40.0	40.0	40.0	40.0
205-209	39.06818878957986	40.0	40.0	40.0	40.0	40.0
210-214	39.2284498976269	40.0	40.0	40.0	40.0	40.0
215-219	39.21908748177306	40.0	40.0	40.0	40.0	40.0
220-224	39.162804191630336	40.0	40.0	40.0	40.0	40.0
225-229	39.26858921569368	40.0	40.0	40.0	40.0	40.0
230-234	39.06396391631849	40.0	40.0	40.0	40.0	40.0
235-239	39.25512280993359	40.0	40.0	40.0	40.0	40.0
240-244	39.170561880945	40.0	40.0	40.0	40.0	40.0
245-249	39.034492554340844	40.0	40.0	40.0	40.0	40.0
250-254	39.17592003566059	40.0	40.0	40.0	40.0	40.0
255-259	39.04569239719248	40.0	40.0	40.0	40.0	40.0
260-264	39.05562296481539	40.0	40.0	40.0	40.0	40.0
265-269	39.08846092823306	40.0	40.0	40.0	40.0	40.0
270-274	37.86382792254399	40.0	36.8	40.0	33.6	40.0
275-279	39.017658896740855	40.0	40.0	40.0	40.0	40.0
280-284	37.96174286210778	40.0	36.8	40.0	36.8	40.0
285-289	38.95196778409907	40.0	40.0	40.0	40.0	40.0
290-294	39.04326322389594	40.0	40.0	40.0	40.0	40.0
295-299	38.924495309595116	40.0	40.0	40.0	40.0	40.0
300-301	38.21424484813444	40.0	40.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1333877048615406
1101	2	0.061871479306184085
1101	3	0.25764355743155676
1101	4	0.2760294313997136
1101	5	0.049251336105307075
1101	6	-0.027254353248828522
1101	7	0.019518290871772592
1101	8	0.12092856012210262
1101	9	-0.05460863657130943
1101	10-14	-0.0600191851156211
1101	15-19	0.007846236871799306
1101	20-24	-0.0866543702643483
1101	25-29	0.05168359343316098
1101	30-34	-0.022936126586728278
1101	35-39	-0.09745174828249503
1101	40-44	0.008454241305479115
1101	45-49	0.015639978077750527
1101	50-54	-0.03312029784130743
1101	55-59	-0.10393038795030662
1101	60-64	-0.06008545145896704
1101	65-69	0.05384459667914854
1101	70-74	-0.057538081441016686
1101	75-79	0.029773832892239227
1101	80-84	-0.05127366365584862
1101	85-89	0.03778737099668916
1101	90-94	-0.11222508011546495
1101	95-99	0.12112493020074311
1101	100-104	-0.024905099402012354
1101	105-109	-0.10806068991475115
1101	110-114	0.040025225671541875
1101	115-119	0.10427457716105692
1101	120-124	-0.03831045848067305
1101	125-129	0.039106147622000265
1101	130-134	0.008376336298823617
1101	135-139	0.08168499886786407
1101	140-144	-0.006882066792520902
1101	145-149	0.21830526783310944
1101	150-154	0.08982509041438647
1101	155-159	0.12126934996013006
1101	160-164	0.03922174586647742
1101	165-169	-0.005282901807014184
1101	170-174	0.2460450450924654
1101	175-179	-0.07215352299697031
1101	180-184	0.0018341670822721312
1101	185-189	-0.01957782576185707
1101	190-194	-0.005284837701537981
1101	195-199	0.1672223607101344
1101	200-204	0.03453751697260543
1101	205-209	0.015106971174382977
1101	210-214	0.10548014252867688
1101	215-219	0.03702485322185822
1101	220-224	0.017143046507172244
1101	225-229	0.08082964256218617
1101	230-234	0.03976664347909775
1101	235-239	-0.03721879232198688
1101	240-244	-0.012797549382135287
1101	245-249	-0.2897998926787011
1101	250-254	0.1287661670991298
1101	255-259	0.019108483553594624
1101	260-264	0.15768840548835072
1101	265-269	0.035684743813618525
1101	270-274	0.21713978363562347
1101	275-279	0.0044344308995860615
1101	280-284	-0.05902255191821126
1101	285-289	0.005683585669508773
1101	290-294	-0.03492024078718714
1101	295-299	-0.09287402614914697
1101	300-301	0.0069293063779269914
1102	1	0.10294373023152303
1102	2	0.07709346662119287
1102	3	0.19062452994741363
1102	4	0.16271019249908392
1102	5	0.007390870989027576
1102	6	0.21735452624377416
1102	7	-0.12720475352568883
1102	8	0.06870868697199484
1102	9	-0.07617311860090581
1102	10-14	0.09207383814019465
1102	15-19	-0.06327427052777779
1102	20-24	-0.03155923284362672
1102	25-29	-0.13262930297275943
1102	30-34	-0.13752386443029252
1102	35-39	-0.09998874616832154
1102	40-44	0.0034648121299980517
1102	45-49	-0.04761583587573881
1102	50-54	0.02024122435742015
1102	55-59	-0.09889867547673248
1102	60-64	-0.014334922917747406
1102	65-69	-0.08378753862739785
1102	70-74	0.041447119404644184
1102	75-79	-0.018006293957654407
1102	80-84	-0.06273243744020363
1102	85-89	0.06391844922078604
1102	90-94	0.03238379937714342
1102	95-99	0.1085245073677541
1102	100-104	-0.02473596620961871
1102	105-109	-0.04353637701833435
1102	110-114	-0.008981116823179036
1102	115-119	-0.03534487315606327
1102	120-124	-0.10245422169420237
1102	125-129	-0.1972152054435341
1102	130-134	-0.05416066158701227
1102	135-139	-0.11831500113213878
1102	140-144	-0.09846769047117476
1102	145-149	-0.01759325225145858
1102	150-154	-0.16049203432133652
1102	155-159	-0.12646849359167334
1102	160-164	-0.1055139835204173
1102	165-169	-0.02392983626790368
1102	170-174	-0.14241161452699203
1102	175-179	-0.06830574287011615
1102	180-184	-0.00484659401709564
1102	185-189	-0.18744251920794852
1102	190-194	-0.13725101106305715
1102	195-199	-0.019965799966392694
1102	200-204	-0.0691411299618565
1102	205-209	-0.08954419161631932
1102	210-214	-0.07016467776730906
1102	215-219	-0.134941320139653
1102	220-224	-0.07824807400022138
1102	225-229	0.066960720786291
1102	230-234	-0.14323547068580922
1102	235-239	-0.07907925743825928
1102	240-244	-0.17368549864217897
1102	245-249	-0.16684006181188948
1102	250-254	-0.022312057002338292
1102	255-259	-0.0464729117952416
1102	260-264	-0.18789298986048664
1102	265-269	-0.0476556367360601
1102	270-274	-0.32945641086754307
1102	275-279	-0.06444506170083741
1102	280-284	-0.14337773162222334
1102	285-289	-0.08873501898165159
1102	290-294	-0.004518549455269749
1102	295-299	-0.08238776822102523
1102	300-301	-0.17625363641623437
1103	1	0.14557695907164714
1103	2	0.16152397863791634
1103	3	0.026047727439575397
1103	4	-0.2640400687339408
1103	5	0.055666733057996964
1103	6	0.1286397927014491
1103	7	4.8594657880585146E-4
1103	8	-0.057205628597493785
1103	9	-0.02413550104604667
1103	10-14	0.02517728641605288
1103	15-19	-0.007892870318791267
1103	20-24	-0.014652231798699233
1103	25-29	-0.04337120474914968
1103	30-34	0.037732143929169126
1103	35-39	0.09620770106260323
1103	40-44	0.041792920802940614
1103	45-49	-0.13037445656539148
1103	50-54	-0.018086884315515306
1103	55-59	0.043901742496096574
1103	60-64	-0.024993230127776656
1103	65-69	0.05963983859307831
1103	70-74	0.08539696266483787
1103	75-79	0.019402274485386783
1103	80-84	0.08821845075205914
1103	85-89	0.024336422052556372
1103	90-94	-0.07219300313069965
1103	95-99	-0.1344430997377799
1103	100-104	-0.07815289410930149
1103	105-109	-0.013818508679783292
1103	110-114	0.10351627084662596
1103	115-119	-0.05645250116024414
1103	120-124	0.0497087877101805
1103	125-129	-0.09696442174447384
1103	130-134	0.09077141782782405
1103	135-139	0.11938614829314531
1103	140-144	0.0654194568642481
1103	145-149	0.16152900480182808
1103	150-154	-0.017461731291035676
1103	155-159	0.07229848655462234
1103	160-164	-0.11305839313378385
1103	165-169	-0.012874454867699114
1103	170-174	-0.04512843793347088
1103	175-179	-0.07204659971442595
1103	180-184	-0.037406677611649286
1103	185-189	-0.12562433738976608
1103	190-194	-0.033405661010817767
1103	195-199	0.09554099209212552
1103	200-204	-0.08226547687930008
1103	205-209	0.007237417579084138
1103	210-214	-0.1608229849773366
1103	215-219	0.008904029912592648
1103	220-224	-0.09638809489886313
1103	225-229	-0.06595463971527948
1103	230-234	-0.0031309774778662813
1103	235-239	-0.007814889622174803
1103	240-244	0.020776361336920957
1103	245-249	-0.0014064149989394537
1103	250-254	-0.08741132554989406
1103	255-259	0.07789490429671275
1103	260-264	-0.017088392159337218
1103	265-269	0.0018114479034423425
1103	270-274	0.04736699561102853
1103	275-279	-0.09660807110522995
1103	280-284	-0.07869643590645126
1103	285-289	-0.08344766265980752
1103	290-294	-0.2843722589641473
1103	295-299	-0.21906488420849257
1103	300-301	-0.7262732114895556
1104	1	-0.040327317427518494
1104	2	-0.08129410423750727
1104	3	-0.02421905602781038
1104	4	-0.058746872375998294
1104	5	-0.1593527779717121
1104	6	0.1535502012385237
1104	7	-0.08618018917548653
1104	8	0.11517100063985453
1104	9	-0.16741813224776791
1104	10-14	-0.026771427448721852
1104	15-19	0.1483822484728421
1104	20-24	0.034344609272686455
1104	25-29	0.08011476589140898
1104	30-34	0.12005329282354893
1104	35-39	0.02999655721189498
1104	40-44	-0.023182259043622366
1104	45-49	0.04919709714966558
1104	50-54	0.007350189295493692
1104	55-59	-0.03255813380130945
1104	60-64	-0.10729314252723299
1104	65-69	0.06103295265960185
1104	70-74	-0.04121926732640446
1104	75-79	-0.11319839977331725
1104	80-84	-0.0045128279503927615
1104	85-89	-0.005424401923455946
1104	90-94	-0.009018678053060114
1104	95-99	-0.11458697699083586
1104	100-104	-0.05995746526483714
1104	105-109	-0.008020945567572824
1104	110-114	0.030258857982616405
1104	115-119	0.09459843988193484
1104	120-124	0.05317458368056549
1104	125-129	-0.1604190685550151
1104	130-134	0.13563988428742846
1104	135-139	0.09406375175470316
1104	140-144	0.15124887471883852
1104	145-149	-0.02912799925965004
1104	150-154	0.1005367290630872
1104	155-159	0.027455503888901944
1104	160-164	0.08636928332821014
1104	165-169	-0.010967417624193843
1104	170-174	-0.16447753936427034
1104	175-179	-0.046223021895947625
1104	180-184	-0.11678444831060375
1104	185-189	0.025137787321547478
1104	190-194	0.005478361181324942
1104	195-199	-0.07774031182866992
1104	200-204	-0.033650892716423186
1104	205-209	-0.03582594681262208
1104	210-214	-0.09494737774631545
1104	215-219	-0.003944049511900971
1104	220-224	-0.11743346136113075
1104	225-229	-0.04016504028866308
1104	230-234	-0.10174901257116886
1104	235-239	0.023738294524775938
1104	240-244	-0.06239849465309533
1104	245-249	-0.12594146848834953
1104	250-254	0.09305640825064643
1104	255-259	-0.12023734597537583
1104	260-264	-0.15089529932930645
1104	265-269	-0.04126890568420549
1104	270-274	-0.05757314401891733
1104	275-279	-0.17360735437351593
1104	280-284	-0.03134329954160364
1104	285-289	-0.10901215524031471
1104	290-294	-0.051887854515108245
1104	295-299	-0.22577172324470496
1104	300-301	-0.11885037313890479
1201	1	0.13032340937590448
1201	2	0.13869774469606
1201	3	0.28762987754099356
1201	4	-0.03707590375760361
1201	5	0.3438614592243212
1201	6	0.33083046207264744
1201	7	-0.008060368498952641
1201	8	0.10560708269392194
1201	9	0.18714238942047956
1201	10-14	-0.06816145597746015
1201	15-19	-0.07016197106253941
1201	20-24	-0.021056559046833456
1201	25-29	0.007798504513864657
1201	30-34	-0.005973062291246833
1201	35-39	0.022449756505473317
1201	40-44	0.08915465170220216
1201	45-49	-0.05138054175809259
1201	50-54	-0.08615449756771909
1201	55-59	-0.08582915676015546
1201	60-64	0.04140839259027729
1201	65-69	0.014030643190785952
1201	70-74	-0.20195395011407413
1201	75-79	0.08368354561454083
1201	80-84	-0.007695004285125151
1201	85-89	0.04779010697480146
1201	90-94	-0.01202261773515545
1201	95-99	-0.04458779209748087
1201	100-104	-0.18311029776042886
1201	105-109	-0.16310856953170827
1201	110-114	-0.04566560880178372
1201	115-119	-0.19615770738066374
1201	120-124	-0.22816545164073432
1201	125-129	-0.07385691667347771
1201	130-134	-0.15878098244262873
1201	135-139	-0.14890323642625702
1201	140-144	-0.03194362630004832
1201	145-149	-0.0680531452995865
1201	150-154	-0.16742251560476262
1201	155-159	-0.17872517808365274
1201	160-164	-0.06467975892148559
1201	165-169	-0.14810095926255684
1201	170-174	-0.2935773899280605
1201	175-179	-0.17014531506264063
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2404	220-224	0.1742194584673058
2404	225-229	0.23727989313936604
2404	230-234	0.15348475823780916
2404	235-239	0.26697401132687304
2404	240-244	0.15016658148108775
2404	245-249	0.11975469935914163
2404	250-254	0.20016207903110939
2404	255-259	0.2142050543064542
2404	260-264	0.1830997462169961
2404	265-269	0.31079032408278806
2404	270-274	0.45843264922490334
2404	275-279	0.3306374828732359
2404	280-284	0.15554808832054334
2404	285-289	0.3519913732701596
2404	290-294	0.26023601801116314
2404	295-299	0.3214511047703894
2404	300-301	0.5781394224379781
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	5.0
17	8.0
18	19.0
19	26.0
20	51.0
21	62.0
22	108.0
23	199.0
24	294.0
25	305.0
26	300.0
27	288.0
28	336.0
29	375.0
30	419.0
31	451.0
32	630.0
33	741.0
34	954.0
35	1255.0
36	1716.0
37	2681.0
38	5835.0
39	105794.0
40	9518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.059681196645765655	0.008310040039283826	0.11936239329153131	99.81264637002342
2	0.009065566711239035	0.021152988992891086	0.15638102576887336	99.813400418527
3	0.013598247337009896	0.12389514240386795	99.60565082722671	0.25685578303240914
4	0.08083402583666995	99.33293042230112	0.10425322958374254	0.4819823222784619
5	0.18055450630807585	0.1306942660723729	0.10954143688146861	99.57920979073809
6	99.75069879882147	0.0876331495051749	0.02493012011785148	0.1367379315554884
7	99.86552844300068	0.074034902168165	0.04457203293797688	0.015864621893178214
8	99.71896955503513	0.13220518244315177	0.0898995240613432	0.058925738460376215
9	30.515977940620985	19.795270831759463	27.068066782503585	22.620684445115963
10-14	59.81385510312004	11.11656719800559	8.889627559114603	20.179950139759764
15-19	35.496864848530635	24.057112638815443	19.9227921734532	20.523230339200726
20-24	59.77094507818992	39.802976505250435	0.35914482133413916	0.06693359522550427
25-29	14.892196116944929	15.085140137493388	59.77940620986628	10.243257535695399
30-34	1.0695776988743673	59.09768074337085	0.336481075772456	39.49626048198232
35-39	56.5267908541072	5.063088408383181	19.743929506579295	18.666191230930316
40-44	38.48756128700829	46.85991433039458	0.20941459102962173	14.443109791567514
45-49	20.202615415996192	19.907229033988322	24.87697270508956	35.01318284492593
50-54	44.70598722958047	20.174301419371997	19.255199881542726	15.864511469504805
55-59	20.486069140577783	26.71990209113985	13.166928562794633	39.62710020548773
60-64	39.97446512752327	0.12027076030460533	0.4405898706636045	59.46467424150852
65-69	27.665901124138763	20.50994197993473	50.713163302308715	1.1109935936177928
70-74	40.18116161005681	58.983289012450136	0.7107457995890245	0.12480357790402513
75-79	44.298773117369755	20.006345944639186	7.658648615979693	28.03623232201136
80-84	39.99818687296023	0.4726217816995044	39.336093315604984	20.193098029735285
85-89	33.578009930102475	2.064439898100105	53.79918588873813	10.558364283059293
90-94	19.71502825541688	59.21307908494757	13.542655102595871	7.529237557039678
95-99	6.766492399746155	12.89006074159137	59.92278867365749	20.420658185004985
100-104	36.76271493759632	20.854915233749356	12.148318273850895	30.234051554803422
105-109	66.83176694569521	28.14269741017195	4.752198482971201	0.27333716116164514
110-114	52.36801547248496	45.99619231815297	0.9978544014988969	0.6379378078631652
115-119	34.89529582951179	43.65602575938666	19.07475933679552	2.373919074306039
120-124	30.664541182514622	32.830268506066695	33.49813390550158	3.007056405917106
125-129	4.674906695275078	49.68495489642042	25.036188634200148	20.60394977410435
130-134	22.240858011903736	11.983971206193932	43.307661273872064	22.46750950803026
135-139	2.837240576907095	20.641729814673507	44.36412538436548	32.15690422405391
140-144	39.801603191272356	18.745249733682883	19.955424936348322	21.497722138696442
145-149	4.977485645210034	28.029162889090358	37.49199153822907	29.50135992747053
150-154	30.32945048269943	17.998365062503872	16.927496112868106	34.744688341928594
155-159	21.74357812027803	73.7561196736174	0.15049864007252944	4.349803566032033
160-164	22.619522514354788	17.931852523420975	4.724690238742823	54.72393472348141
165-169	38.45421577515866	22.21260199456029	21.118162586884253	18.215019643396797
170-174	2.9295859776367483	26.37654880628589	20.634632819582954	50.059232396494416
175-179	43.98428528256271	2.145210033242672	18.764883650649743	35.10562103354488
180-184	38.65488062858869	39.04820187367785	0.6719552734965246	21.624962224236928
185-189	39.023597870646135	1.5042377043111408	6.198426405680292	53.27373801936244
190-194	21.907539343754486	36.6693613581245	37.15848563376877	4.264613664352254
195-199	31.507793198800233	40.29948851230366	9.451114015669505	18.741604273226603
200-204	76.2736194743085	0.7840796621310224	20.677248997045915	2.265051866514555
205-209	21.1314682039	40.282867050975	2.8400033242922658	35.74566142083274
210-214	29.296836633700767	28.467123505012882	41.392576250953844	0.8434636103325047
215-219	39.663339855997705	19.902386690742603	15.781322010592405	24.65295144266729
220-224	8.312392810462455	30.903376423212624	45.94428788371021	14.839942882614707
225-229	55.98319721363867	2.2404218829093603	22.050483911180955	19.725896992271018
230-234	20.265490068676854	57.01690100408737	20.927477542139183	1.7901313850965934
235-239	44.16684925090096	20.607136651077752	3.910123225470123	31.315890872551165
240-244	37.95576871352717	23.272347588524525	8.104032372611417	30.667851325336887
245-249	54.23250402314916	4.735001019953309	20.944401211855634	20.088093745041892
250-254	18.465083598395275	11.095732062043382	62.02766717790252	8.411517161658821
255-259	17.740236780271836	39.66757077342682	19.968721431863344	22.623471014438007
260-264	38.944386101436244	31.837502549883272	26.365566376294776	2.852544972385709
265-269	21.566648282323076	57.39738136431976	18.514192461411767	2.521777891945391
270-274	7.575178341263809	3.408618707057947	26.006621405787005	63.00958154589124
275-279	48.70864599734657	7.853747283865177	18.69637616994013	24.74123054884812
280-284	2.9415897912464017	37.611913234660804	6.414167743300317	53.03232923079248
285-289	53.93080834409967	26.704141073007094	16.44310463368012	2.921945949213113
290-294	23.716791158110432	20.115480106806256	24.75191119425652	31.415817540826797
295-299	23.581956433009328	26.493174706353646	30.82765188734864	19.097216973288386
300-301	8.789500752395284	59.571801367645094	31.605173431874896	0.03352444808472542
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.25
41	0.25
42	0.0
43	0.0
44	0.0
45	0.5833333333333333
46	2.083333333333333
47	3.666666666666667
48	10.75
49	169.83333333333331
50	7378.333333333321
51	5172.999999999998
52	278.7500000000002
53	392.66666666666663
54	11664.25000000001
55	18661.00000000005
56	1965.5833333333267
57	169.5
58	41.583333333333336
59	17.41666666666667
60	4.5
61	1.0833333333333333
62	1.75
63	0.75
64	2.25
65	0.5
66	0.0
67	0.5
68	0.25
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.554581853894387E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.511030522816561E-4
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	1.5110419389690162E-4
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	3.0220838779380324E-4
275-279	0.0
280-284	0.0
285-289	0.0
290-294	3.022239146761444E-4
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-39	1.0
40-49	0.0
50-59	1.0
60-69	0.0
70-79	0.0
80-89	4.0
90-99	0.0
100-109	0.0
110-119	2.0
120-129	0.0
130-139	1.0
140-149	1.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	1.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	2.0
280-289	0.0
290-299	778.0
300-302	131579.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.788622799728035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.30807577268195	2.1711868248092467
2	13.858424725822532	1.05008687769132
3	6.719840478564307	0.7637682254287226
4	3.848454636091725	0.5832137191206467
5	2.831505483549352	0.5363753116265014
6	1.7547357926221334	0.39888192188562366
7	1.7347956131605182	0.4600740349021682
8	1.296111665004985	0.39283825640250813
9	1.2362911266201395	0.4215456674473068
>10	8.235294117647058	5.901639344262295
>50	0.15952143569292124	0.40341467099796025
>100	0.3988035892323031	3.438845659892725
>500	0.259222333000997	6.645010198685503
>1k	0.21934197407776668	24.75258744428496
>5k	0.07976071784646062	26.44859107048425
>10k+	0.05982053838484546	25.631940772078266
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	13224	9.990179043589938	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10504	7.935332779330665	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10201	7.706428949157663	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9419	7.115660648183122	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8910	6.731132431819899	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8535	6.447835612298859	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8146	6.153962378182368	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4621	3.4909722746845966	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	4020	3.0369419052655435	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3960	2.991614414142177	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3686	2.784618871345471	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3340	2.5232303392007256	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3245	2.4514618115887283	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3115	2.3532522474881015	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2903	2.1930951121855404	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1545	1.1671828964266828	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1271	0.9601873536299765	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1059	0.8000302183274155	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	835	0.6308075848001814	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	825	0.6232530029462869	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	822	0.6209866283901186	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	790	0.5968119664576566	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	769	0.5809473445644784	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	753	0.5688600135982473	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	704	0.5318425625141648	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	646	0.4880259877615774	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	547	0.413235627408023	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	536	0.4049255873687391	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	534	0.40341467099796025	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	522	0.394349172773287	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	513	0.387550049104782	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	456	0.34448893253758406	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	356	0.2689431139986402	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	351	0.265165823071693	No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	314	0.23721387021228374	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	294	0.222104706504495	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	279	0.2107728337236534	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	272	0.20548462642592732	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	256	0.1933972954596963	No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	226	0.17073354989801315	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	213	0.16091259348795045	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	201	0.15184709526327717	No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	193	0.14580342978016167	No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	185	0.13975976429704615	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	177	0.13371609881393065	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	163	0.12313968421847851	No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	161	0.12162876784769963	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
