FastQCFastQC Report
Mon 14 Oct 2024
IVFS1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIVFS1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228943
Sequences flagged as poor quality0
Sequence length51-301
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2600711.359596056660392No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT219709.596275055363126No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT196768.594278925322023No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT172447.53200578309885No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT167767.327588089611825No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT146956.418628217503921No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT145156.340006027701219No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC79643.478595108826214No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC65332.8535486998947337No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC60042.622486819863459No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC51752.2603879568276817No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC50972.2263183412465115No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT48052.0987756777887947No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC44641.9498303071070093No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC44191.930174759656334No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT21740.9495813368393006No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT20450.8932354341473643No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT16330.7132779774878463No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT15320.6691621932096635No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC14630.6390236871186278No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT10370.4529511712522331No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT9840.4298013042547708No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC6450.2817295134596821No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5760.2515910073686463No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC5730.2502806375386013No Hit
CCTACGGGCGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5570.24329199844502783No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT5510.24067125878493772No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5130.22407324093770062No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC5120.2236364509943523No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4620.2017969538269351No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC4310.1882564655831364No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3780.16510659858567417No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT3710.16204906898223576No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3690.16117548909553905No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT3410.14894537068178543No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3270.1428303114749086No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3220.1406463617581669No Hit
CCTACGGGCGGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCA3080.13453130255129006No Hit
CCTACGGGGGGCAGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2980.1301634031178066No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2970.12972661317445827No Hit
CCTACGGGAGGCAGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC2720.11880686459074966No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2710.11837007464740132No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2690.11749649476070463No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT2340.10220884674351258No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)