##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IVFS1_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	228943
Sequences flagged as poor quality	0
Sequence length	51-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.333170265087816	34.0	34.0	34.0	20.0	34.0
2	33.11860157331738	34.0	34.0	34.0	34.0	34.0
3	33.63787492956762	34.0	34.0	34.0	34.0	34.0
4	33.40165892820484	34.0	34.0	34.0	34.0	34.0
5	33.204570569967196	34.0	34.0	34.0	34.0	34.0
6	33.171680287233066	34.0	34.0	34.0	34.0	34.0
7	33.56379972307518	34.0	34.0	34.0	34.0	34.0
8	33.49659085449217	34.0	34.0	34.0	34.0	34.0
9	33.393320608186315	34.0	34.0	34.0	34.0	34.0
10-14	33.20806488951398	34.0	34.0	34.0	31.2	34.0
15-19	33.404849241951055	34.0	34.0	34.0	34.0	34.0
20-24	33.64412976155637	34.0	34.0	34.0	34.0	34.0
25-29	33.54067518989443	34.0	34.0	34.0	34.0	34.0
30-34	33.466359748933144	34.0	34.0	34.0	34.0	34.0
35-39	33.561061050130384	34.0	34.0	34.0	34.0	34.0
40-44	33.63467063854322	34.0	34.0	34.0	34.0	34.0
45-49	33.63318380557606	34.0	34.0	34.0	34.0	34.0
50-54	33.45403143390672	34.0	34.0	34.0	34.0	34.0
55-59	33.39364290363019	34.0	34.0	34.0	34.0	34.0
60-64	33.36019236397151	34.0	34.0	34.0	34.0	34.0
65-69	33.14038289340922	34.0	34.0	34.0	34.0	34.0
70-74	33.44865795117519	34.0	34.0	34.0	34.0	34.0
75-79	33.49320567307734	34.0	34.0	34.0	34.0	34.0
80-84	33.57807340280358	34.0	34.0	34.0	34.0	34.0
85-89	33.53531049333769	34.0	34.0	34.0	34.0	34.0
90-94	33.444395425835815	34.0	34.0	34.0	34.0	34.0
95-99	33.48007058679642	34.0	34.0	34.0	34.0	34.0
100-104	33.192127965335445	34.0	34.0	34.0	34.0	34.0
105-109	33.303072024181326	34.0	34.0	34.0	34.0	34.0
110-114	33.211112227381335	34.0	34.0	34.0	34.0	34.0
115-119	33.29540424023281	34.0	34.0	34.0	34.0	34.0
120-124	33.18587640213859	34.0	34.0	34.0	34.0	34.0
125-129	33.088533913408114	34.0	34.0	34.0	34.0	34.0
130-134	33.29018677709054	34.0	34.0	34.0	34.0	34.0
135-139	33.01837806199113	34.0	34.0	34.0	34.0	34.0
140-144	33.023265890904	34.0	34.0	34.0	31.2	34.0
145-149	33.21573540203376	34.0	34.0	34.0	34.0	34.0
150-154	33.22291752208059	34.0	34.0	34.0	34.0	34.0
155-159	33.23539710134798	34.0	34.0	34.0	34.0	34.0
160-164	33.3174731582028	34.0	34.0	34.0	34.0	34.0
165-169	33.336251496064364	34.0	34.0	34.0	34.0	34.0
170-174	32.4515406186936	34.0	34.0	34.0	28.4	34.0
175-179	33.264115421911995	34.0	34.0	34.0	34.0	34.0
180-184	32.9810635379629	34.0	34.0	34.0	34.0	34.0
185-189	32.77529891491925	34.0	34.0	34.0	31.2	34.0
190-194	33.137960014502056	34.0	34.0	34.0	34.0	34.0
195-199	32.874424641961966	34.0	34.0	34.0	34.0	34.0
200-204	32.98610417067077	34.0	34.0	34.0	34.0	34.0
205-209	32.254437363222976	34.0	34.0	34.0	31.2	34.0
210-214	32.532507174650874	34.0	34.0	34.0	31.2	34.0
215-219	32.90320402217262	34.0	34.0	34.0	34.0	34.0
220-224	32.97734164442561	34.0	34.0	34.0	34.0	34.0
225-229	33.213620697939554	34.0	34.0	34.0	34.0	34.0
230-234	32.41283006670132	34.0	34.0	34.0	28.4	34.0
235-239	32.157318143894884	34.0	34.0	34.0	31.2	34.0
240-244	32.91546186405511	34.0	34.0	34.0	34.0	34.0
245-249	32.653773407708	34.0	34.0	34.0	31.2	34.0
250-254	32.885640651550034	34.0	34.0	34.0	34.0	34.0
255-259	32.45248568345921	34.0	34.0	34.0	31.2	34.0
260-264	32.79011226138995	34.0	34.0	34.0	34.0	34.0
265-269	32.597373869741844	34.0	34.0	34.0	34.0	34.0
270-274	32.59055169702529	34.0	34.0	34.0	34.0	34.0
275-279	32.216466168697856	34.0	34.0	34.0	25.6	34.0
280-284	32.02547611084242	34.0	34.0	34.0	25.6	34.0
285-289	32.271173063272634	34.0	34.0	34.0	28.4	34.0
290-294	32.04270659542918	34.0	34.0	34.0	28.4	34.0
295-299	31.567889120373515	34.0	34.0	34.0	25.6	34.0
300-301	31.858797442872756	34.0	34.0	34.0	27.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.13503493432796887
1101	2	-0.2380142021342948
1101	3	-0.09342768312053806
1101	4	-0.22136449867088004
1101	5	0.2892713606516537
1101	6	-0.28267052925856007
1101	7	-0.16884450112740979
1101	8	-0.10890839780073236
1101	9	-0.17130434077517265
1101	10-14	0.01926477674382454
1101	15-19	-0.16936481499251954
1101	20-24	-0.10535960635229458
1101	25-29	-0.19950621807200974
1101	30-34	-0.1340831413788237
1101	35-39	-0.16376280710282032
1101	40-44	-0.18318776758355426
1101	45-49	-0.2010134944373263
1101	50-54	-0.22896088590022146
1101	55-59	-0.20938028369988615
1101	60-64	-0.22102122255961376
1101	65-69	-0.20151907923563783
1101	70-74	-0.17640659997127273
1101	75-79	-0.2817246375450111
1101	80-84	-0.18098070332339944
1101	85-89	-0.20904361702286423
1101	90-94	-0.2224906333475758
1101	95-99	-0.260031124469009
1101	100-104	-0.26594202088085694
1101	105-109	-0.36645391301533436
1101	110-114	-0.29571619156381246
1101	115-119	-0.19667966071705933
1101	120-124	-0.16089150359588444
1101	125-129	-0.20264781419814426
1101	130-134	-0.23755922927865925
1101	135-139	-0.24279527946355017
1101	140-144	-0.15237562135560268
1101	145-149	-0.08841263894241536
1101	150-154	-0.037010141669362895
1101	155-159	-0.18964212673657954
1101	160-164	-0.1588818573254258
1101	165-169	-0.2297425878456778
1101	170-174	-0.3294313158055928
1101	175-179	-0.2540265241185935
1101	180-184	-0.38056115325125006
1101	185-189	-0.22566137942892084
1101	190-194	-0.17314691472461874
1101	195-199	-0.3534240983682366
1101	200-204	-0.023561953366758814
1101	205-209	-0.3616529199258771
1101	210-214	-0.20818043515636475
1101	215-219	-0.24735895464063873
1101	220-224	-0.3820223615656104
1101	225-229	-0.3003512569258362
1101	230-234	-0.571551198637767
1101	235-239	-0.31649019249030275
1101	240-244	-0.4064329306654031
1101	245-249	-0.414435256387236
1101	250-254	-0.43979828396204823
1101	255-259	-0.5462595804303518
1101	260-264	-0.34586165716070383
1101	265-269	-0.5943775957183419
1101	270-274	-0.6635813388471874
1101	275-279	-0.6355468598313649
1101	280-284	-0.6903854056841716
1101	285-289	-0.4860435006014008
1101	290-294	-0.6135692526578218
1101	295-299	-0.999430848374459
1101	300-301	-0.6180075317195985
1102	1	0.07254157518402593
1102	2	-0.25157086052966804
1102	3	-0.03824985020077065
1102	4	-0.08720403547815891
1102	5	-0.25752764679251783
1102	6	-0.0071783869922228405
1102	7	0.09295037066745948
1102	8	0.05608746654996821
1102	9	0.07509764433648769
1102	10-14	0.03336734084638948
1102	15-19	-0.039907412180276935
1102	20-24	-0.02870286524395027
1102	25-29	0.07277004330102699
1102	30-34	0.008581871028134458
1102	35-39	0.11940013747947376
1102	40-44	0.04666169478203841
1102	45-49	0.019160202833162998
1102	50-54	0.031136715423180306
1102	55-59	0.014507226639231874
1102	60-64	0.0941202166463313
1102	65-69	0.008232781806533751
1102	70-74	0.10369761839100988
1102	75-79	-0.01961545640356377
1102	80-84	-0.015404193811413336
1102	85-89	0.0685461265668863
1102	90-94	-0.06609857379422834
1102	95-99	0.06382991771462088
1102	100-104	-0.005199258267133189
1102	105-109	0.03786370485067181
1102	110-114	-0.07631337932229343
1102	115-119	0.01927402001081191
1102	120-124	-0.0997351760524623
1102	125-129	0.020918769755532196
1102	130-134	0.024536717785032636
1102	135-139	-0.06112613140730616
1102	140-144	-0.07832103078488473
1102	145-149	-0.1236831104064322
1102	150-154	-0.11800269750063563
1102	155-159	-0.07369671730729976
1102	160-164	-0.015351667085560905
1102	165-169	-0.02998907254377059
1102	170-174	-0.21585315203387978
1102	175-179	0.045460655368586345
1102	180-184	-0.049052468387728254
1102	185-189	-0.13483259530982394
1102	190-194	0.005208751114921029
1102	195-199	-0.21012550448899958
1102	200-204	0.17753151230734687
1102	205-209	-0.08656276277120156
1102	210-214	0.08020169883867112
1102	215-219	-0.06906945918984775
1102	220-224	-0.15790821764501572
1102	225-229	0.145673556969939
1102	230-234	-0.05482828714065846
1102	235-239	-0.07758862094357255
1102	240-244	-0.13994326978863825
1102	245-249	0.010170202669833373
1102	250-254	-0.17804642291986283
1102	255-259	-0.444830300033324
1102	260-264	-0.3142638270228204
1102	265-269	-0.289215808480769
1102	270-274	-0.256059702340508
1102	275-279	-0.4761756229271441
1102	280-284	-0.6871408821014171
1102	285-289	-0.6298980701487871
1102	290-294	-0.651537864194534
1102	295-299	-0.8684349328263572
1102	300-301	-0.6143932239559469
1103	1	-0.08177242531098017
1103	2	0.12797373150069546
1103	3	-0.10251352481508036
1103	4	0.05946703126551256
1103	5	-0.3001049918960561
1103	6	-0.18139503578358074
1103	7	-0.1531877367494232
1103	8	-0.23902184786303593
1103	9	-0.20888057528235748
1103	10-14	-0.20857639316896126
1103	15-19	-0.021860716363100607
1103	20-24	-0.03815192060240946
1103	25-29	0.026522373966813007
1103	30-34	-0.02925725115651545
1103	35-39	0.0204564859655747
1103	40-44	-0.026734229646642405
1103	45-49	-7.754460406985686E-4
1103	50-54	-0.08419139281870969
1103	55-59	-0.12099809470388578
1103	60-64	0.0044853680364838056
1103	65-69	-0.0227762108508216
1103	70-74	-0.03256044612511744
1103	75-79	-0.07018224132230699
1103	80-84	0.08095188241992446
1103	85-89	-0.009827274967648236
1103	90-94	-0.07901433982013373
1103	95-99	-0.09199649924767783
1103	100-104	-0.13191421577888462
1103	105-109	-0.156247342092648
1103	110-114	-0.11972497902528545
1103	115-119	-0.16873291011046376
1103	120-124	-0.015365239578571277
1103	125-129	-0.2114785571976867
1103	130-134	-0.07214969422717843
1103	135-139	-0.11967179595642818
1103	140-144	-0.28495480687595887
1103	145-149	-0.07381692774823279
1103	150-154	-0.1926780543193729
1103	155-159	-0.1530987468428151
1103	160-164	-0.09040917059186881
1103	165-169	-0.15194666680167757
1103	170-174	-0.2324610226716075
1103	175-179	-0.11817105818640528
1103	180-184	-0.1073740590155694
1103	185-189	-0.21598992556310037
1103	190-194	-0.12103726819042038
1103	195-199	-0.26388773532804066
1103	200-204	0.19068488249133253
1103	205-209	-0.2815479950127653
1103	210-214	-0.20715544991143275
1103	215-219	-0.16918727125155897
1103	220-224	-0.21419037258272766
1103	225-229	-0.21312324197404564
1103	230-234	-0.47449762135400775
1103	235-239	-0.4379473372211038
1103	240-244	-0.3165758908595109
1103	245-249	-0.4929872925863279
1103	250-254	-0.4206462084161089
1103	255-259	-0.4027341033498857
1103	260-264	-0.24083127195309828
1103	265-269	-0.35898793949306196
1103	270-274	-0.46552756802815054
1103	275-279	-0.3569465164177785
1103	280-284	-0.567860954702688
1103	285-289	-0.5793101648604448
1103	290-294	-0.6155906651185497
1103	295-299	-0.9140901009993065
1103	300-301	-0.41167655173644846
1104	1	0.42593855545908355
1104	2	0.026240469747492057
1104	3	-0.04625254187759964
1104	4	-0.16982169119587098
1104	5	0.26163120419793273
1104	6	0.10809435557411717
1104	7	0.07094254798167299
1104	8	-0.01781756772685128
1104	9	0.04316762793408202
1104	10-14	0.0466659066873234
1104	15-19	-0.02212013876479091
1104	20-24	-0.0383669944444307
1104	25-29	-0.07408683519500414
1104	30-34	-0.018998395616101504
1104	35-39	0.020609205065802882
1104	40-44	0.027694457535965
1104	45-49	-0.03322992207746722
1104	50-54	-0.03947370641304815
1104	55-59	0.02484831256261799
1104	60-64	-0.0642415615426728
1104	65-69	-0.1495634077884418
1104	70-74	-0.06489421405211715
1104	75-79	-0.03240260364670178
1104	80-84	-0.07929013400571705
1104	85-89	-0.05026482519322428
1104	90-94	-0.1061803334779583
1104	95-99	-0.2545813200361522
1104	100-104	-0.2257377618630514
1104	105-109	-0.15000019723958502
1104	110-114	-0.23843240190714
1104	115-119	-0.20326810052583966
1104	120-124	-0.22713661441727595
1104	125-129	-0.22365607147759192
1104	130-134	-0.13153141528474066
1104	135-139	-0.11363839115411167
1104	140-144	-0.16111095381975105
1104	145-149	-0.20417535080682114
1104	150-154	-0.2647024493616712
1104	155-159	-0.23586116193866502
1104	160-164	-0.19401440839018136
1104	165-169	-0.21218935012294082
1104	170-174	-0.5892748620898125
1104	175-179	-0.07704260408382879
1104	180-184	-0.31564589901395834
1104	185-189	-0.3548139218018136
1104	190-194	-0.22448111724525432
1104	195-199	-0.3018117559084388
1104	200-204	0.05190523481228837
1104	205-209	-0.27087499728354913
1104	210-214	-0.24274366853315854
1104	215-219	-0.24416034534033315
1104	220-224	-0.18787633809755278
1104	225-229	-0.07842166109793425
1104	230-234	-0.3245151273643998
1104	235-239	-0.11796781091707942
1104	240-244	-0.16933167034379437
1104	245-249	-0.46165820293222026
1104	250-254	-0.2534227950441874
1104	255-259	-0.4833391110692027
1104	260-264	-0.32569874311819547
1104	265-269	-0.4625638338834719
1104	270-274	-0.5044055828132272
1104	275-279	-0.5313250530486862
1104	280-284	-0.7345268433244527
1104	285-289	-0.9674801032059435
1104	290-294	-0.654608394538986
1104	295-299	-1.1810669984009223
1104	300-301	-0.6008100409973096
1201	1	-0.572827722579472
1201	2	-0.15377015871594324
1201	3	-0.23052651700836435
1201	4	0.07000223856741883
1201	5	0.1668396639080285
1201	6	-0.2756956998066684
1201	7	-0.09095526498129658
1201	8	-0.038968551714098965
1201	9	0.06233816350952992
1201	10-14	0.03389158724219499
1201	15-19	-0.10745097959244276
1201	20-24	-0.06306535625988374
1201	25-29	-0.14251021713105416
1201	30-34	-0.15698968437710192
1201	35-39	-0.11540847030811818
1201	40-44	-0.0591335954559824
1201	45-49	-3.3499862119867885E-4
1201	50-54	-0.15017034921978478
1201	55-59	-0.2090872434068416
1201	60-64	-0.11963499410917677
1201	65-69	-0.031799349424169066
1201	70-74	-0.1266626741723087
1201	75-79	-0.08804483111291006
1201	80-84	-0.0798875151659999
1201	85-89	-0.034719928478985196
1201	90-94	-0.16166091260959803
1201	95-99	-0.08575347551245471
1201	100-104	-0.2334162286303041
1201	105-109	-0.14069123532604522
1201	110-114	-0.21749022792501194
1201	115-119	-0.08339270302643342
1201	120-124	-0.04321532656190641
1201	125-129	-0.06438051574735937
1201	130-134	-0.1539120202186055
1201	135-139	-0.2306089031487346
1201	140-144	-0.23539810346065337
1201	145-149	-0.20010030907045007
1201	150-154	-0.10958600617183123
1201	155-159	-0.14456197752798516
1201	160-164	-0.1631255646884
1201	165-169	-0.22765803631158832
1201	170-174	-0.3980571853488897
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2404	265-269	0.5130088856237833
2404	270-274	0.47213607074070296
2404	275-279	0.5738621968726321
2404	280-284	0.6645743570531977
2404	285-289	0.5335724526543189
2404	290-294	0.5349181980969036
2404	295-299	0.9471625740458904
2404	300-301	0.44726715810230644
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	29.0
16	90.0
17	114.0
18	151.0
19	290.0
20	637.0
21	954.0
22	877.0
23	858.0
24	772.0
25	908.0
26	975.0
27	1268.0
28	1814.0
29	2499.0
30	3837.0
31	6669.0
32	19627.0
33	179452.0
34	7121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.22625719065444239	0.07032318087908344	0.1043927964602543	99.59902683200622
2	0.041059510081420135	0.17166369640423523	0.2463570604885208	99.54091973302582
3	0.11007106572378277	0.17908387677282117	99.563646846595	0.14719821090839205
4	0.08997872832975894	99.55840536727483	0.16991128796250593	0.18170461643291125
5	0.17995745665951787	0.22669398059779072	0.18694609575309137	99.40640246698959
6	99.47454169815194	0.19830263428014835	0.051978003258452976	0.27517766430945695
7	99.81305390424691	0.06944960099238674	0.056782692635284765	0.06071380212541986
8	99.56888832591518	0.13060019306115497	0.10482958640360264	0.19568189462005828
9	30.307980589054917	18.86757839287508	27.65404489327038	23.170396124799623
10-14	59.716086536823575	11.423891536321268	8.643461472943047	20.21656045391211
15-19	35.669489785667174	23.15266245309968	19.92041687232193	21.257430888911212
20-24	59.418457869426014	39.90102339883727	0.5592658434632202	0.12125288827350038
25-29	15.33324888727762	15.42051951795862	59.29598196931114	9.950249625452622
30-34	9.60317633646803	54.91017414815042	0.46203640207387864	35.02461311330768
35-39	59.98462499399414	0.8644946558750431	19.72045443625706	19.43042591387376
40-44	39.2454016938714	58.93519347610541	0.2688878891252408	1.5505169408979529
45-49	20.245301232184428	19.969686777931624	20.494708289836336	39.29030370004761
50-54	39.972953824945904	20.41907593982401	15.105673144877867	24.502297090352222
55-59	20.454877020717127	39.30645886931568	0.28015951708082	39.95850459288638
60-64	39.97414180946183	0.26688098680446054	0.5140188956980183	59.2449583080357
65-69	35.96044395717674	24.434504959793134	24.910260722194803	14.694790360835325
70-74	40.089193285606335	40.788325376407016	18.961042364626692	0.16143897335994864
75-79	39.630123044801934	19.78186519671007	2.144045845872954	38.44396591261504
80-84	40.152231892869835	0.18607479158312956	39.56710092853942	20.094592387007612
85-89	38.79902960711668	1.5894245696000058	44.556653280230215	15.054892543053104
90-94	15.519660344722153	63.40362893010335	5.341184076038054	15.735526649136448
95-99	19.688998768225456	0.4672007268343394	59.37485258017454	20.468947924765658
100-104	1.8714318786391015	29.05646531578557	24.782975228993127	44.2891275765822
105-109	45.063139641036614	54.04639704372819	0.5342081009185934	0.3562552143166024
110-114	25.400350314715403	54.884182111235845	15.023958556284041	4.691509017764712
115-119	30.97305627239705	21.1332055540316	28.286458597234866	19.607279576336484
120-124	25.345424048642418	24.473477303700598	34.68209455917811	15.499004088478877
125-129	48.967222280462664	29.446220777859317	20.091728692735085	1.494828248942936
130-134	40.03686619841353	49.40987874340427	5.110685955900339	5.442569102281861
135-139	28.54850263829192	5.836915120382989	6.457874689869659	59.15670755145543
140-144	39.51488625642101	20.087273299087954	19.899709962609638	20.4981304818814
145-149	5.2383198797917325	35.48581262885697	20.51927176154034	38.75659572981095
150-154	34.463557182419386	5.839849039461155	28.32615513641486	31.370438641704595
155-159	35.0486166318677	48.69132588431601	0.12588781046065678	16.13416967335564
160-164	35.3189128744529	5.240462316650214	0.5584142154507412	58.882210593446146
165-169	24.919234364489327	35.285453449465784	34.58970707714887	5.205605108896013
170-174	15.858544383970926	39.10830195602226	20.33171132291403	24.701442337092786
175-179	83.22940236050565	1.227690076615968	5.4529252972472415	10.089982265631143
180-184	25.71439803611521	24.948675164021072	14.858605536966987	34.478321262896735
185-189	24.990848866963056	14.945394599477401	44.864226888693395	15.19952964486615
190-194	34.81944499045572	24.927817308994335	10.526311191483972	29.726426509065973
195-199	5.568975322867954	39.607814701794325	35.314323953862406	19.508886021475316
200-204	64.54915997373517	0.5687197125987679	20.087496692723462	14.794623620942598
205-209	25.15316842192626	49.62001651152531	15.362794903267796	9.864020163280639
210-214	64.3184190869738	5.373060004979666	29.392524385076722	0.915996522969803
215-219	40.396189244794286	19.99108901808842	15.426744302868551	24.18597743424875
220-224	45.1643029247266	9.600717771483785	44.18662757479553	1.0483517289940891
225-229	15.69749461409299	1.4217396698041505	63.12510155907375	19.75566415702911
230-234	20.172471331948344	44.43692155009287	20.27844221922083	15.112164898737959
235-239	58.943437105503406	33.82023404431903	1.9462632845704602	5.2900655656071045
240-244	25.73421686010195	49.46791828105412	15.266084540756822	9.531780318087108
245-249	43.28259893382629	16.245258392492403	24.187134278131207	16.285008395550097
250-254	35.06812096221132	6.166836295652402	28.64714695694336	30.117895785192918
255-259	5.526119223696223	39.54239487007002	5.9016909024990065	49.02979500373475
260-264	39.312977766129386	20.477525881273753	38.985628794828116	1.2238675577687503
265-269	20.410780587952647	43.8697418424846	5.887476521207356	29.832001048355394
270-274	19.863364347180358	20.40501463329402	44.41610972786441	15.315511291661208
275-279	23.39579784213515	32.176647883632555	19.917966190538593	24.509588083693707
280-284	29.128157638394438	53.62972799426896	6.444880290395711	10.797234076940885
285-289	29.92302363107256	20.537165931207884	34.43371129886593	15.10609913885363
290-294	43.48208973609651	16.438600307699566	20.163756975003995	19.915552981199923
295-299	54.41869937530647	34.69317294841886	9.879807461185003	1.008320215089666
300-301	73.31598823167786	26.252579809423437	0.32384841698502614	0.1075835419136697
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.5
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.5833333333333333
22	0.0
23	0.25
24	0.25
25	1.0
26	2.25
27	3.0
28	2.25
29	2.5
30	3.5
31	2.833333333333333
32	3.833333333333333
33	3.75
34	3.75
35	3.5833333333333335
36	3.0
37	2.0
38	5.0
39	5.5
40	6.25
41	4.25
42	6.0
43	14.000000000000004
44	18.833333333333332
45	21.083333333333336
46	25.083333333333332
47	30.666666666666664
48	62.0
49	113.16666666666667
50	605.5
51	661.8333333333336
52	5618.083333333323
53	12090.250000000051
54	6911.499999999995
55	38888.66666666667
56	8221.666666666666
57	158.00000000000006
58	46.833333333333336
59	25.33333333333333
60	16.083333333333336
61	8.416666666666666
62	5.916666666666666
63	4.166666666666667
64	2.3333333333333335
65	1.0
66	0.25
67	0.5
68	0.0
69	0.5
70	0.25
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0030575296034384106
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.6130204602239966
205-209	0.0
210-214	0.0
215-219	0.0
220-224	8.736256776059161E-5
225-229	8.736256776059161E-5
230-234	8.736256776059161E-5
235-239	0.0
240-244	0.0
245-249	8.736256776059161E-5
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	1.7481249175103555E-4
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	2.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	2.0
85-89	1.0
90-94	0.0
95-99	1.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	1.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	2.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	1.0
190-194	0.0
195-199	1.0
200-204	1.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	1.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	2.0
285-289	1.0
290-294	31.0
295-299	713.0
300-302	228183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.342316646501531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.09513852587559	2.04199298515351
2	11.761630946157867	0.7862218980270199
3	4.9660219550444324	0.4979405354171126
4	3.5938316779926813	0.4804689376831788
5	2.469942498693152	0.4127664964641854
6	1.7250392054364874	0.34593763513188874
7	1.3068478829064296	0.3057529603438411
8	1.150026136957658	0.30750012011723443
9	0.9409304756926294	0.2830398832897272
>10	9.043387349712493	5.869583258715052
>50	1.2545739675901726	2.8766985668921956
>100	0.31364349189754315	2.741293684454209
>500	0.10454783063251437	2.145075411783719
>1k	0.11761630946157868	10.296012544607173
>5k	0.06534239414532149	13.4413369266586
>10k+	0.09147935180345008	57.16837815526136
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	26007	11.359596056660392	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	21970	9.596275055363126	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	19676	8.594278925322023	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	17244	7.53200578309885	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	16776	7.327588089611825	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	14695	6.418628217503921	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	14515	6.340006027701219	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	7964	3.478595108826214	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	6533	2.8535486998947337	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	6004	2.622486819863459	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	5175	2.2603879568276817	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	5097	2.2263183412465115	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4805	2.0987756777887947	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	4464	1.9498303071070093	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	4419	1.930174759656334	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2174	0.9495813368393006	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2045	0.8932354341473643	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1633	0.7132779774878463	No Hit
CCTACGGGCGGCTGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1532	0.6691621932096635	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	1463	0.6390236871186278	No Hit
CCTACGGGGGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1037	0.4529511712522331	No Hit
CCTACGGGAGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	984	0.4298013042547708	No Hit
CCTACGGGGGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	645	0.2817295134596821	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	576	0.2515910073686463	No Hit
CCTACGGGAGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	573	0.2502806375386013	No Hit
CCTACGGGCGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	557	0.24329199844502783	No Hit
CCTACGGGTGGCAGCCGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	551	0.24067125878493772	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	513	0.22407324093770062	No Hit
CCTACGGGTGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	512	0.2236364509943523	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	462	0.2017969538269351	No Hit
CCTACGGGCGGCTGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	431	0.1882564655831364	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	378	0.16510659858567417	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	371	0.16204906898223576	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	369	0.16117548909553905	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	341	0.14894537068178543	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	327	0.1428303114749086	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	322	0.1406463617581669	No Hit
CCTACGGGCGGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCA	308	0.13453130255129006	No Hit
CCTACGGGGGGCAGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	298	0.1301634031178066	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	297	0.12972661317445827	No Hit
CCTACGGGAGGCAGCCGTAGGGAATCTTCGGCAATGGGCGAAAGCCTGAC	272	0.11880686459074966	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	271	0.11837007464740132	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	269	0.11749649476070463	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	234	0.10220884674351258	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	5.241479320180132E-4	0.0	0.0	0.0
160-164	0.0	0.001310369830045033	0.0	0.0	0.0
165-169	0.0	0.001310369830045033	0.0	0.0	0.0
170-174	0.0	0.001310369830045033	0.0	0.0	0.0
175-179	0.0	0.001310369830045033	0.0	0.0	0.0
180-184	0.0	0.001310369830045033	0.0	0.0	0.0
185-189	0.0	0.001310369830045033	0.0	0.0	0.0
190-194	0.0	0.001310369830045033	0.0	0.0	0.0
195-199	0.0	0.001310369830045033	0.0	0.0	0.0
200-204	0.0	0.001310369830045033	0.0	0.0	0.0
205-209	0.0	0.001310369830045033	0.0	0.0	0.0
210-214	0.0	0.001310369830045033	0.0	0.0	0.0
215-219	0.0	0.001310369830045033	0.0	0.0	0.0
220-224	0.0	0.001310369830045033	0.0	0.0	0.0
225-229	0.0	0.001310369830045033	0.0	0.0	0.0
230-234	0.0	0.001310369830045033	0.0	0.0	0.0
235-239	0.0	0.001310369830045033	0.0	0.0	0.0
240-244	0.0	0.001310369830045033	0.0	0.0	0.0
245-249	0.0	0.001310369830045033	0.0	0.0	0.0
250-254	0.0	0.001310369830045033	0.0	0.0	0.0
255-259	0.0	0.001310369830045033	0.0	0.0	0.0
260-264	0.0	0.001310369830045033	0.0	0.0	0.0
265-269	0.0	0.001310369830045033	0.0	0.0	0.0
270-274	0.0	0.001310369830045033	0.0	0.0	0.0
275-279	0.0	0.001310369830045033	0.0	0.0	0.0
280-284	0.0	0.001310369830045033	0.0	0.0	0.0
285-289	0.0	0.001310369830045033	0.0	0.0	0.0
290	0.0	0.001310369830045033	0.0	0.0	0.0
>>END_MODULE
