FastQCFastQC Report
Mon 14 Oct 2024
IPFS2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIPFS2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224428
Sequences flagged as poor quality0
Sequence length42-301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG48822.175307893845688No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG48752.172188853440747No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG47102.098668615324291No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG41851.864740584953749No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG41771.8611759673481028No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG40241.7930026556401164No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG38811.7292851159391875No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG38361.7092341419074268No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG37921.689628745076372No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA37831.6856185502700196No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG37261.6602206498297893No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG36931.6455166022064982No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA36911.6446254478050866No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA36511.626802359776855No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG35281.5719963640900423No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG32081.429411659864188No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG31801.4169354982444258No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA31761.4151531894416025No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA31641.4098062630331332No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA31101.3857450941950202No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG31031.3826260537900796No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA29721.3242554404976206No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG29461.31267043327927No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA29451.312224856078564No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG27921.2440515443705775No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG27911.2436059671698718No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA27661.232466537152227No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20180.8991747910242929No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20000.8911544014115886No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19720.8786782397918262No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17400.775304329228082No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17220.7672839396153777No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16750.7463418111822054No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16090.716933715935623No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16060.7155969843335056No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15030.6697025326608088No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG10380.4625091343326145No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG10230.4558254763220275No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG10000.4455772007057943No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9420.41973372306485823No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9340.4161691054592119No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9130.40681198424439025No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA9030.4023562122373322No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8990.40057390343450905No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG8970.39968274903309753No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8900.3965637086281569No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG8730.3889888962161584No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG8310.3702746537865151No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG8240.3671556133815745No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG8170.36403657297663394No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8150.36314541857522237No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8130.36225426417381074No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC8120.361808686973105No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA8070.359580800969576No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8040.3582440693674586No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7970.355125028962518No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC7950.35423387456110644No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG7910.3524515657582833No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC7890.35156041135687166No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG7880.3511148341561659No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7830.34888694815263693No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7760.34576790774769633No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG7730.344431176145579No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7530.33551963213146313No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG7470.33284616892722835No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG7410.33017270572299356No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7330.3266080881173472No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG7280.32438020211381824No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG7280.32438020211381824No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7150.3185876985046429No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7020.3127951948954676No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6810.3034380736806459No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG6700.2985367244728822No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG6690.2980911472721764No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG6620.29497210686723585No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG6600.2940809524658242No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6560.29229864366300107No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG6530.2909619120608837No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6480.2887340260573547No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG6480.2887340260573547No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG6430.28650614005382574No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6390.28472383125100253No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA6360.28338709964888514No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6360.28338709964888514No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG6210.27670344163829824No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG6160.2744755556347693No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG6090.27135651522982873No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG6090.27135651522982873No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6000.2673463204234766No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5930.264227280018536No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5860.26110823961359547No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5820.25932593081077226No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5800.2584347764093607No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5780.2575436220079491No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5650.2517511183987738No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5480.24417630598677525No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5450.2428395743846579No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5390.2401661111804231No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5320.23704707077548257No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5300.236155916374071No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG5190.23125456716630724No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5030.22412533195501455No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5020.22367975475430873No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG5020.22367975475430873No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG5020.22367975475430873No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4990.22234302315219134No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4930.21966955994795656No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4900.21833282834583922No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4850.2161049423423102No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4850.2161049423423102No Hit
GACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG4740.2112035931345465No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4720.21031243873313488No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG4540.2022920491204306No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG4530.2018464719197248No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4530.2018464719197248No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4500.2005097403176074No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG4480.19961858591619583No Hit
GACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG4440.19783627711337268No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG4380.1951628139091379No Hit
GACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG4340.19338050510631472No Hit
GACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG4190.1866968470957278No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA4180.18625126989502203No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG4080.18179549788796406No Hit
GACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG4050.18045876628584667No Hit
GACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG3980.17733972588090613No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3910.17422068547596556No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3870.17243837667314238No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3720.16575471866255548No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3610.16085336945479173No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3600.16040779225408594No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3580.15951663785267434No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3570.15907106065196855No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3540.15773432904985116No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3530.15728875184914537No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3310.14748605343361793No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3270.14570374463079475No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3200.14258470422585418No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3160.140802395423031No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3100.1381289322187962No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3020.13456431461314988No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2810.1252071933983282No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2800.12476161619762241No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2730.12164257579268184No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2670.11896911258844707No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2530.11273103177856596No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2480.11050314577503698No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2390.10649295096868483No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2380.10604737376797904No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG2360.10515621936656744No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG2260.10070044735950952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)