##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IPFS1_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	160962
Sequences flagged as poor quality	0
Sequence length	42-301
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.167169891030184	34.0	34.0	34.0	20.0	34.0
2	33.061412010288144	34.0	34.0	34.0	34.0	34.0
3	33.564251189721794	34.0	34.0	34.0	34.0	34.0
4	33.37900249748388	34.0	34.0	34.0	34.0	34.0
5	33.13371478982617	34.0	34.0	34.0	34.0	34.0
6	33.02221021110573	34.0	34.0	34.0	34.0	34.0
7	33.48738211503336	34.0	34.0	34.0	34.0	34.0
8	33.42260906300866	34.0	34.0	34.0	34.0	34.0
9	33.36524148556802	34.0	34.0	34.0	34.0	34.0
10-14	33.315942893353714	34.0	34.0	34.0	34.0	34.0
15-19	33.47495930716567	34.0	34.0	34.0	34.0	34.0
20-24	33.71096407847815	34.0	34.0	34.0	34.0	34.0
25-29	33.62709956387222	34.0	34.0	34.0	34.0	34.0
30-34	33.51816702078751	34.0	34.0	34.0	34.0	34.0
35-39	33.59240938855133	34.0	34.0	34.0	34.0	34.0
40-44	33.23210964187108	34.0	34.0	34.0	34.0	34.0
45-49	33.5929658668721	34.0	34.0	34.0	34.0	34.0
50-54	33.537056871979026	34.0	34.0	34.0	34.0	34.0
55-59	33.355213161197334	34.0	34.0	34.0	34.0	34.0
60-64	33.268544589271734	34.0	34.0	34.0	31.2	34.0
65-69	33.522946358677416	34.0	34.0	34.0	34.0	34.0
70-74	33.562082323370035	34.0	34.0	34.0	34.0	34.0
75-79	33.34115161907602	34.0	34.0	34.0	34.0	34.0
80-84	33.60471184671588	34.0	34.0	34.0	34.0	34.0
85-89	33.56818316156044	34.0	34.0	34.0	34.0	34.0
90-94	32.97912322775452	34.0	34.0	34.0	34.0	34.0
95-99	33.20094261777624	34.0	34.0	34.0	34.0	34.0
100-104	33.217555435437674	34.0	34.0	34.0	34.0	34.0
105-109	33.360916540853545	34.0	34.0	34.0	34.0	34.0
110-114	33.19203866967376	34.0	34.0	34.0	34.0	34.0
115-119	33.13625140570732	34.0	34.0	34.0	34.0	34.0
120-124	33.2795534077258	34.0	34.0	34.0	34.0	34.0
125-129	33.31542375161864	34.0	34.0	34.0	34.0	34.0
130-134	33.13625403034689	34.0	34.0	34.0	34.0	34.0
135-139	33.25327625414735	34.0	34.0	34.0	34.0	34.0
140-144	33.213749584692934	34.0	34.0	34.0	34.0	34.0
145-149	33.342975550653954	34.0	34.0	34.0	34.0	34.0
150-154	32.89422473515798	34.0	34.0	34.0	31.2	34.0
155-159	33.10306489435373	34.0	34.0	34.0	34.0	34.0
160-164	33.224973747491255	34.0	34.0	34.0	34.0	34.0
165-169	33.25826876355344	34.0	34.0	34.0	34.0	34.0
170-174	32.8582369776888	34.0	34.0	34.0	31.2	34.0
175-179	33.21598647909728	34.0	34.0	34.0	34.0	34.0
180-184	33.03539804450177	34.0	34.0	34.0	34.0	34.0
185-189	33.18006151551869	34.0	34.0	34.0	34.0	34.0
190-194	33.095365209556654	34.0	34.0	34.0	34.0	34.0
195-199	32.47197564234008	34.0	34.0	34.0	28.4	34.0
200-204	32.96861217261627	34.0	34.0	34.0	34.0	34.0
205-209	32.72041922205579	34.0	34.0	34.0	34.0	34.0
210-214	32.90546376442371	34.0	34.0	34.0	34.0	34.0
215-219	32.946107077312305	34.0	34.0	34.0	34.0	34.0
220-224	32.931735113000144	34.0	34.0	34.0	34.0	34.0
225-229	33.046084887001356	34.0	34.0	34.0	34.0	34.0
230-234	32.71123552915297	34.0	34.0	34.0	31.2	34.0
235-239	32.85209567070784	34.0	34.0	34.0	34.0	34.0
240-244	32.54946279990306	34.0	34.0	34.0	31.2	34.0
245-249	32.8548124304809	34.0	34.0	34.0	34.0	34.0
250-254	32.22940587968458	34.0	34.0	34.0	31.2	34.0
255-259	32.42851852081999	34.0	34.0	34.0	31.2	34.0
260-264	32.50148700963791	34.0	34.0	34.0	31.2	34.0
265-269	32.88281767509492	34.0	34.0	34.0	34.0	34.0
270-274	32.31981716144145	34.0	34.0	34.0	28.4	34.0
275-279	32.47089140561468	34.0	34.0	34.0	31.2	34.0
280-284	32.04453954745337	34.0	34.0	34.0	25.6	34.0
285-289	31.759066144194776	34.0	34.0	34.0	22.8	34.0
290-294	31.80638037255426	34.0	34.0	34.0	22.8	34.0
295-299	31.665167410045	34.0	34.0	34.0	20.0	34.0
300-301	31.44239455036634	34.0	34.0	34.0	20.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.23995675247602577
1101	2	0.03629101656362366
1101	3	0.11188554990759059
1101	4	-0.04094685297176426
1101	5	0.28612788146136836
1101	6	-0.038202912306125825
1101	7	0.21365289513158103
1101	8	0.11866307197946213
1101	9	0.23468337800893124
1101	10-14	-0.07999368958963515
1101	15-19	-0.09788791122203833
1101	20-24	-0.14266265615420082
1101	25-29	-0.01670712677935171
1101	30-34	-0.08661085825769987
1101	35-39	0.034737259233878603
1101	40-44	-0.11734569065362876
1101	45-49	-0.0076383384807741095
1101	50-54	-0.032820714869579604
1101	55-59	-0.1413450525225386
1101	60-64	-0.2136650776489617
1101	65-69	-0.10540302489676634
1101	70-74	-0.14184367629993488
1101	75-79	-0.04306456317338814
1101	80-84	-0.0692387758149593
1101	85-89	-0.011906327379101356
1101	90-94	-0.20693183546362803
1101	95-99	-0.1286135935197663
1101	100-104	0.029643620717656916
1101	105-109	-0.10333279660128625
1101	110-114	-0.1044534758324076
1101	115-119	-0.1783440961981384
1101	120-124	-0.04580216892772171
1101	125-129	0.04318897802712485
1101	130-134	0.05164166010253979
1101	135-139	-0.012217046714532387
1101	140-144	0.13848315046957538
1101	145-149	-0.009812704923675142
1101	150-154	0.0975979319581981
1101	155-159	-0.16114485777776366
1101	160-164	-0.09794036931493366
1101	165-169	0.10982454783586348
1101	170-174	-0.09712708827235161
1101	175-179	-8.470541521887753E-5
1101	180-184	0.014512273484953653
1101	185-189	0.032949517520933114
1101	190-194	-0.05588927385399245
1101	195-199	-0.0687363876402145
1101	200-204	0.05074599048254669
1101	205-209	-0.18296368975630628
1101	210-214	-0.08496316358814937
1101	215-219	-0.0026722948664072987
1101	220-224	-0.24339049605087126
1101	225-229	-0.17851980298475922
1101	230-234	-0.33699972919210097
1101	235-239	-0.09861757187361064
1101	240-244	-0.20018526091845246
1101	245-249	-0.16456123561267333
1101	250-254	-0.2594368683771435
1101	255-259	-0.35424622970182895
1101	260-264	-0.18989938832945086
1101	265-269	-0.19332739426666734
1101	270-274	-0.3387568763922033
1101	275-279	-0.09047178608508233
1101	280-284	-0.4269807581222942
1101	285-289	-0.45710608278904985
1101	290-294	-0.27013808098500647
1101	295-299	-0.35134128381572793
1101	300-301	-0.6817227387827316
1102	1	-0.15025700872013914
1102	2	-0.16329432961104118
1102	3	-0.08042803460110548
1102	4	-0.2975249716417423
1102	5	0.09876767393019037
1102	6	0.12319547197379421
1102	7	0.076830750302328
1102	8	0.150984282305636
1102	9	-0.20801575285213403
1102	10-14	-0.08550130008738677
1102	15-19	-0.0032971350568118396
1102	20-24	0.007732762908297275
1102	25-29	-0.044726558996387666
1102	30-34	-0.10832877790745243
1102	35-39	-0.09348677638957525
1102	40-44	-0.27062870720914844
1102	45-49	-0.08654308044557979
1102	50-54	-0.18231329161287135
1102	55-59	-0.20498249937161717
1102	60-64	-0.07803650943023399
1102	65-69	-0.11156223426290524
1102	70-74	-0.16344312342815925
1102	75-79	-0.05303055338919194
1102	80-84	-0.08001160475492952
1102	85-89	-0.10266989639785606
1102	90-94	-0.308216989971541
1102	95-99	-0.2287848339747356
1102	100-104	-0.06794096968403807
1102	105-109	-0.059640266838471234
1102	110-114	-0.23766084700205425
1102	115-119	-0.11373708112694203
1102	120-124	-0.11586232437183952
1102	125-129	-0.2123794007036537
1102	130-134	-0.22778122892479047
1102	135-139	-0.1203116477038364
1102	140-144	-0.1330272720483805
1102	145-149	-0.2390168623559461
1102	150-154	-0.1256526408292089
1102	155-159	-0.32691855483535903
1102	160-164	-0.1680379729698771
1102	165-169	-0.1710424803762507
1102	170-174	-0.4265998515077456
1102	175-179	-0.22886059149094962
1102	180-184	-0.3991413441824321
1102	185-189	-0.19558329562271126
1102	190-194	-0.28121583927431004
1102	195-199	-0.36271369402445686
1102	200-204	0.06320671241596187
1102	205-209	-0.23471167010621485
1102	210-214	-0.17759641964197925
1102	215-219	-0.36974714703109157
1102	220-224	-0.39961626110079607
1102	225-229	-0.37407844518072864
1102	230-234	-0.5309269911491441
1102	235-239	-0.37397018082237565
1102	240-244	-0.38990198222546724
1102	245-249	-0.2518632281979265
1102	250-254	-0.7025969526696585
1102	255-259	-0.6222133705990167
1102	260-264	-0.7346687243726677
1102	265-269	-0.40772452913811463
1102	270-274	-0.680256643191985
1102	275-279	-0.7504958210276698
1102	280-284	-0.9693145987086673
1102	285-289	-1.0772872640417894
1102	290-294	-0.8799209154060392
1102	295-299	-1.1657886611307546
1102	300-301	-1.2538545563047876
1103	1	0.27573069273880435
1103	2	0.07632626441652945
1103	3	-0.03209798355813831
1103	4	0.16427653687211574
1103	5	0.11535663739628887
1103	6	-0.3883896733177963
1103	7	-0.21597634480370687
1103	8	-0.35583484339938565
1103	9	-0.10999919403215586
1103	10-14	-0.12567385478106985
1103	15-19	0.019709710863104135
1103	20-24	0.08721844810315815
1103	25-29	0.08232487248412923
1103	30-34	0.05676372909253047
1103	35-39	-0.0010708767070966019
1103	40-44	-0.010436190831185854
1103	45-49	0.10235206042496969
1103	50-54	0.1132040375405623
1103	55-59	0.015482246150412493
1103	60-64	0.0748793701565944
1103	65-69	0.03297241734541245
1103	70-74	-0.027420991413102058
1103	75-79	-0.051991608009188894
1103	80-84	-0.02152922864464557
1103	85-89	0.12677358792082316
1103	90-94	0.16783635729478164
1103	95-99	0.16468273833613267
1103	100-104	0.3805116648563853
1103	105-109	0.010018642905244235
1103	110-114	0.0373435070565975
1103	115-119	0.12254696426244749
1103	120-124	0.27340659568763925
1103	125-129	0.10116775040227566
1103	130-134	0.21147094475525563
1103	135-139	0.20059252281450313
1103	140-144	0.24600291162591503
1103	145-149	0.16614839091629818
1103	150-154	0.056441460426107426
1103	155-159	0.16471562385520855
1103	160-164	0.23227216997721456
1103	165-169	0.17785158872600704
1103	170-174	0.18153152048962795
1103	175-179	0.19503649195960548
1103	180-184	0.1356971537343128
1103	185-189	0.16990978477330998
1103	190-194	0.05716887195112719
1103	195-199	-0.21215503480657816
1103	200-204	0.40239658956452473
1103	205-209	0.01583091258741831
1103	210-214	0.1175002404601102
1103	215-219	0.06903143903860354
1103	220-224	0.12672445403659793
1103	225-229	0.09211597358594048
1103	230-234	0.10603092854861274
1103	235-239	0.0032132121280739057
1103	240-244	-0.1522534418398962
1103	245-249	0.07787231297808006
1103	250-254	-0.19037343539409335
1103	255-259	-0.0444827059687114
1103	260-264	-0.12463496367173832
1103	265-269	-0.07020125167068869
1103	270-274	-0.07834889564701086
1103	275-279	0.0923152932094311
1103	280-284	-0.1994808567636781
1103	285-289	-0.012944994840395907
1103	290-294	-0.007533685525139333
1103	295-299	-0.25980898487538795
1103	300-301	-0.6050275697123375
1104	1	0.23863862726075524
1104	2	-0.19633821269742668
1104	3	-0.06630974458410321
1104	4	-0.34820912677088245
1104	5	0.05389276501593798
1104	6	-0.09587585828286649
1104	7	-0.04044720289984127
1104	8	-0.03836273413028124
1104	9	0.20364119800204605
1104	10-14	-0.09416254209209285
1104	15-19	0.0070076985599030195
1104	20-24	-0.09280970328877203
1104	25-29	-0.1835128920868172
1104	30-34	-0.16427161654399924
1104	35-39	-0.0768059408297006
1104	40-44	-0.17684480307182326
1104	45-49	-0.1150913761301453
1104	50-54	-0.09180847418918603
1104	55-59	-0.16763734513309458
1104	60-64	-0.0754699156321621
1104	65-69	-0.2650882645438557
1104	70-74	-0.2242722060935307
1104	75-79	-0.31267349728037885
1104	80-84	-0.1589262675352714
1104	85-89	-0.29746788846327377
1104	90-94	-0.38610744418576814
1104	95-99	-0.2887658071752881
1104	100-104	-0.21600590369747152
1104	105-109	-0.2718680216761271
1104	110-114	-0.3858553251819572
1104	115-119	-0.5184654432028779
1104	120-124	-0.23255732882491742
1104	125-129	-0.4288760406518364
1104	130-134	-0.35827673536151394
1104	135-139	-0.2951456692405543
1104	140-144	-0.4696680298817526
1104	145-149	-0.28836104501322524
1104	150-154	-0.46138254124213063
1104	155-159	-0.47598138375609267
1104	160-164	-0.3565976037690035
1104	165-169	-0.33906531253558825
1104	170-174	-0.6182078184739765
1104	175-179	-0.4561964347425089
1104	180-184	-0.49641889255907756
1104	185-189	-0.24592537318727636
1104	190-194	-0.31838894266674345
1104	195-199	-0.5835318464007777
1104	200-204	-0.062380284588329005
1104	205-209	-0.1791696087346537
1104	210-214	-0.2891805019025284
1104	215-219	-0.35582519739138974
1104	220-224	-0.29783502881191026
1104	225-229	-0.36310767358328633
1104	230-234	-0.5100622838379962
1104	235-239	-0.3659505872671929
1104	240-244	-0.535803123833432
1104	245-249	-0.4184732192917693
1104	250-254	-0.7821026607094872
1104	255-259	-0.5981788794646938
1104	260-264	-0.7249164775910302
1104	265-269	-0.5180273386272134
1104	270-274	-0.7750020079397757
1104	275-279	-0.7038477763349249
1104	280-284	-1.191262214286354
1104	285-289	-1.4121447654586845
1104	290-294	-1.120814365329121
1104	295-299	-1.2798806374889402
1104	300-301	-1.5729662646017815
1201	1	-0.37812066226741337
1201	2	-0.16251724724030936
1201	3	-0.11162684167465642
1201	4	-0.07698759467978533
1201	5	-0.028568823738559956
1201	6	-0.09928111054394861
1201	7	-0.3243362734021815
1201	8	-0.19706111050600583
1201	9	-0.11981690377413656
1201	10-14	-0.13137786300305976
1201	15-19	-0.0018486815207339191
1201	20-24	-0.02942297559998508
1201	25-29	-0.055747827250812065
1201	30-34	-0.11103526479507053
1201	35-39	-0.07481999771103887
1201	40-44	-0.13965672417894126
1201	45-49	-0.029927379181245328
1201	50-54	-0.11875355428072254
1201	55-59	-0.17153295371977606
1201	60-64	-0.1581221985132828
1201	65-69	-0.13690912103290742
1201	70-74	-0.22661501964916653
1201	75-79	-0.14894352016366952
1201	80-84	-0.059610042189305545
1201	85-89	-0.11512071618475517
1201	90-94	-0.3149867075616015
1201	95-99	-0.1906209886071295
1201	100-104	-0.13465034087737138
1201	105-109	-0.08247878772168349
1201	110-114	-0.39993944853381436
1201	115-119	-0.21886862507059845
1201	120-124	-0.1471808441349296
1201	125-129	-0.1245935954082995
1201	130-134	-0.06786047732511946
1201	135-139	-0.0687399811071785
1201	140-144	-0.16659513024134753
1201	145-149	-0.07433140569754215
1201	150-154	-0.066553076275369
1201	155-159	-0.5033078521008605
1201	160-164	-0.051443413946621774
1201	165-169	-0.20152581160936478
1201	170-174	-0.13789834914849308
1201	175-179	-0.2765489464565789
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2404	270-274	0.7033289878526503
2404	275-279	0.5410446967833522
2404	280-284	0.7598948684625952
2404	285-289	0.8052523827414113
2404	290-294	0.8421356112954754
2404	295-299	0.9296857486728172
2404	300-301	1.0360582221823904
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	22.0
16	68.0
17	80.0
18	129.0
19	262.0
20	456.0
21	516.0
22	581.0
23	529.0
24	577.0
25	617.0
26	717.0
27	926.0
28	1226.0
29	1678.0
30	2727.0
31	4895.0
32	14419.0
33	126289.0
34	4248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.29199438376759734	0.07020290503348617	0.1112063716902126	99.5265963395087
2	0.060885070111022066	0.1627743711131406	0.28889344491454344	99.4874471138613
3	0.14786098582274076	0.2342167716603919	99.45266584659733	0.1652563959195338
4	0.09629602017867571	99.46695493346256	0.22489780196568135	0.21185124439308656
5	0.20750239186888833	0.2609311514518955	0.1969408928815497	99.33462556379766
6	99.36444626682074	0.25596103428138317	0.05653508281457736	0.3230576160832992
7	99.76391943440066	0.08511325654502305	0.06647531715560194	0.08449199189870901
8	99.48000149103515	0.17146904238267419	0.11431269492178278	0.2342167716603919
9	30.907294889477022	22.19840707744685	24.995340515152645	21.898957517923485
10-14	59.68588859482362	10.539754724717636	9.530448180315851	20.24390850014289
15-19	22.219281569563	37.87415663324263	19.83847119195835	20.06809060523602
20-24	59.50013046557573	39.719933897441635	0.5268324200743033	0.2531032169083386
25-29	1.806761844410482	2.2069805295659846	59.276226687044144	36.710030938979386
30-34	3.0030690473527915	58.12440203277792	0.5103067804823499	38.36222213938694
35-39	42.55650401958226	18.091599259452543	19.69856239360843	19.653334327356767
40-44	39.265061468535116	41.562272459561484	0.589461755810746	18.583204316092655
45-49	20.307160874265335	19.962971706904906	37.68287379316344	22.04699362566632
50-54	56.691807800792745	20.300451049342065	18.44108400949316	4.566657140372023
55-59	20.090085612395782	38.59727382298488	1.4756644590514296	39.83697610556791
60-64	40.02447843536823	0.22713999925446388	0.5617614532983759	59.18662011207893
65-69	39.22961269399471	20.348538127958847	39.99440847923061	0.4274406988158401
70-74	40.10392786183708	59.36500126120015	0.4194888349617104	0.1115820420010711
75-79	40.27398792216507	19.82603941449837	36.746564278436345	3.1534083849002212
80-84	39.83150674718557	0.649991301970725	39.45376376152489	20.06473818931882
85-89	21.379719970376705	18.450194589274975	41.19141819049371	18.978667249854617
90-94	18.949762043813763	59.93265156504343	2.1302980975931014	18.98728829354971
95-99	18.791673604501185	1.502300047467214	59.568619946667724	20.137406401363876
100-104	38.30782899355712	3.7673109541294667	54.360092697868325	3.5647673544450864
105-109	42.68301926649394	40.17011177175946	16.727491876510534	0.4193770852360627
110-114	55.728690984324615	42.00692127515486	0.5556901704224215	1.708697570098103
115-119	23.821162962640805	54.88813365558462	20.192853725668062	1.097849656106517
120-124	55.921660045554574	22.868869672407172	20.141559666430155	1.0679106156080964
125-129	1.7593238072870652	40.39063499933519	37.61968136332062	20.230359830057125
130-134	36.34986554529955	3.915092229462728	23.58760432044457	36.14743790479315
135-139	1.7551228362307856	19.196127893827743	71.11950592124067	7.9292433487008065
140-144	40.067799474603774	19.854459778856405	19.745727117055992	20.332013629483825
145-149	18.018577775016308	37.64627667836839	39.00922675448135	5.325918792133959
150-154	3.8128553232470717	37.735872503028986	2.967597626518376	55.48367454720557
155-159	21.150789656487934	77.33203101214464	0.29053692167557044	1.2266424096918545
160-164	20.835855024015608	19.68969223982055	16.984907137575572	42.48954559858827
165-169	38.93088599886912	21.071102356822855	20.32124371648533	19.676767927822688
170-174	1.4459109984925742	38.12544815784601	20.18235752464014	40.24628331902128
175-179	40.2947755629567	1.1909081871054332	19.75170254014018	38.762613709797684
180-184	39.62698169083572	38.94260941319458	0.3395142659371004	21.090894630032597
185-189	40.14042936589306	0.7194208842079101	1.8132786528722775	57.32687109702675
190-194	20.12700779817939	39.528753844719915	38.946655482026905	1.3975828750737875
195-199	21.495013514773046	39.217572311802904	19.69378941808805	19.593624755336005
200-204	77.73566407857075	0.7484178184714769	20.310821608954505	1.205096494003279
205-209	21.130427542256506	39.72937940942176	1.1204607111744083	38.019732337147325
210-214	38.440966116334124	3.644000919637365	56.5365711196585	1.3784618443700174
215-219	39.941242044747014	19.869459906519175	2.825054650615107	37.3642433981187
220-224	2.3499804261506627	21.03946460861622	74.70549124147917	1.9050637237539536
225-229	59.2982691616986	0.8025959467537616	20.042776947177554	19.856357944370085
230-234	20.295289168380695	58.68399957744816	20.10613507988094	0.9145761742902061
235-239	57.383285589118046	20.21587427839952	1.0470586042118475	21.35378152827058
240-244	40.07071529326962	20.876049388853332	1.354900047847782	37.69833527002927
245-249	57.78321847794341	18.25138106097796	3.7810932907467363	20.184307170331888
250-254	19.659348648766212	50.20922530091284	27.34295674436235	2.788469305958602
255-259	19.956377736489216	38.7340843985161	20.60897176980867	20.700566095186016
260-264	39.53196169691848	21.197934467180772	38.05663437459221	1.2134694613085437
265-269	21.123863615179552	58.55971962442597	19.030616366427015	1.2858003939674512
270-274	18.49506171151745	1.1960762138172654	20.023215610633944	60.28564646403134
275-279	39.64512264235362	2.3480765980293703	20.47523290613162	37.531567853485384
280-284	1.8798394213201755	39.29057035260195	17.511900471047365	41.317689755030514
285-289	58.18453897571148	37.86071704670026	2.3680736982616977	1.5866702793265537
290-294	23.347245595762185	19.188233159243616	20.272295871314473	37.19222537367972
295-299	38.53879972390292	20.805034418859172	21.779706864494784	18.876458992743125
300-301	2.4805452051268366	94.35740318120034	3.0342887816699573	0.1277628320028629
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.5
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.25
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.25
20	0.25
21	0.5
22	0.5
23	0.75
24	1.75
25	1.5
26	1.25
27	1.5
28	1.75
29	2.583333333333333
30	2.333333333333333
31	2.5
32	3.75
33	2.75
34	3.5
35	2.75
36	2.0
37	2.25
38	2.75
39	3.25
40	4.25
41	4.75
42	5.5
43	7.5
44	14.25
45	17.166666666666668
46	30.499999999999993
47	31.41666666666666
48	46.41666666666667
49	79.66666666666667
50	810.4166666666677
51	3470.4166666666856
52	31077.833333333103
53	8680.583333333303
54	3081.666666666669
55	2822.583333333335
56	347.6666666666666
57	51.58333333333334
58	37.83333333333333
59	23.583333333333332
60	11.416666666666668
61	8.75
62	8.25
63	3.5833333333333335
64	2.25
65	2.333333333333333
66	1.833333333333333
67	0.0
68	1.0
69	1.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	1.5
76	1.25
77	0.25
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018637939389421108
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.6198775903315003
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	2.4858060474689523E-4
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40-49	4.0
50-59	0.0
60-69	0.0
70-79	2.0
80-89	2.0
90-99	1.0
100-109	0.0
110-119	2.0
120-129	4.0
130-139	1.0
140-149	1.0
150-159	8.0
160-169	0.0
170-179	1.0
180-189	1.0
190-199	0.0
200-209	2.0
210-219	4.0
220-229	2.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	5.0
280-289	8.0
290-299	1144.0
300-302	159770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.24137374038593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.48322835799033	2.692560977125036
2	12.34802988135345	1.0474521936854662
3	5.287827742785997	0.672829611958102
4	2.7537717884868904	0.46719101402815566
5	2.050681119085982	0.4348852524198258
6	1.4794199501977443	0.3764863756663063
7	1.171817782334847	0.34790820193586064
8	0.9228065035886919	0.3131173817422746
9	0.8056247253552072	0.30752599992544827
>10	7.953713197597774	6.936419776096221
>50	0.9228065035886919	2.3987027994184964
>100	0.4687271129339388	4.699867049365689
>500	0.17577266735022704	6.551235695381519
>1k	0.05859088911674235	2.8217840235583553
>5k	0.0	0.0
>10k+	0.1171817782334847	69.93203364769325
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	18638	11.57913047800102	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	15792	9.81101129459127	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	14482	8.997154607919882	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	14228	8.839353387756116	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	13843	8.600166498925212	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	12979	8.063393844509886	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	11929	7.411065965880145	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	10673	6.630757570109716	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1254	0.7790658664778022	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC	1171	0.7275009008337372	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1109	0.6889824927622669	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC	1008	0.6262347634845492	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	998	0.6200221170214089	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC	989	0.6144307352045825	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	977	0.606975559448814	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	964	0.5988991190467315	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC	964	0.5988991190467315	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC	906	0.5628657695605174	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	892	0.5541680645121209	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC	878	0.5454703594637244	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	813	0.505088157453312	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	750	0.46594848473552763	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC	718	0.4460680160534785	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC	696	0.4324001938345696	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	418	0.2596886221592674	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	357	0.22179147873411117	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	353	0.219306420148855	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	323	0.2006684807594339	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	310	0.19259204035735142	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	308	0.19134951106472334	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	305	0.18948571712578124	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	303	0.18824318783315316	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	294	0.18265180601632683	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	289	0.17954548278475665	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	282	0.1751966302605584	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	273	0.16960524844373207	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	269	0.1671201898584759	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	268	0.16649892521216186	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	258	0.1602862787490215	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	246	0.15283110299325306	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	245	0.15220983834693902	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	240	0.14910351511536885	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	233	0.1447546625911706	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	222	0.1379207514817162	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	216	0.13419316360383196	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	209	0.1298443110796337	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	204	0.1267379878480635	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	185	0.11493395956809682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	1.2425292926280737E-4	0.0
75-79	0.0	0.0	0.0	6.212646463140369E-4	0.0
80-84	0.0	0.0	0.0	6.212646463140369E-4	0.0
85-89	0.0	0.0	0.0	6.212646463140369E-4	0.0
90-94	0.0	0.0	0.0	6.212646463140369E-4	0.0
95-99	0.0	0.0	0.0	6.212646463140369E-4	0.0
100-104	0.0	0.0	0.0	6.212646463140369E-4	0.0
105-109	0.0	0.0	0.0	6.212646463140369E-4	0.0
110-114	0.0	0.0	0.0	6.212646463140369E-4	0.0
115-119	0.0	0.0	0.0	6.212646463140369E-4	0.0
120-124	0.0	0.0	0.0	6.212646463140369E-4	0.0
125-129	0.0	0.0	0.0	6.212646463140369E-4	0.0
130-134	0.0	0.0	0.0	6.212646463140369E-4	0.0
135-139	0.0	0.0	0.0	6.212646463140369E-4	0.0
140-144	0.0	0.0	0.0	6.212646463140369E-4	0.0
145-149	0.0	0.0	0.0	6.212646463140369E-4	0.0
150-154	0.0	0.0	0.0	6.212646463140369E-4	0.0
155-159	0.0	0.0	0.0	6.212646463140369E-4	0.0
160-164	0.0	0.0	0.0	6.212646463140369E-4	0.0
165-169	0.0	0.0	0.0	6.212646463140369E-4	0.0
170-174	0.0	0.0	0.0	6.212646463140369E-4	0.0
175-179	0.0	0.0	0.0	6.212646463140369E-4	0.0
180-184	0.0	0.0	0.0	6.212646463140369E-4	0.0
185-189	0.0	0.0	0.0	6.212646463140369E-4	0.0
190-194	0.0	0.0	0.0	6.212646463140369E-4	0.0
195-199	0.0	0.0	0.0	6.212646463140369E-4	0.0
200-204	0.0	0.0	0.0	6.212646463140369E-4	0.0
205-209	0.0	0.0	0.0	6.212646463140369E-4	0.0
210-214	0.0	0.0	0.0	6.212646463140369E-4	0.0
215-219	0.0	0.0	0.0	6.212646463140369E-4	0.0
220-224	0.0	0.0	0.0	6.212646463140369E-4	0.0
225-229	0.0	0.0	0.0	6.212646463140369E-4	0.0
230-234	0.0	0.0	0.0	6.212646463140369E-4	0.0
235-239	0.0	0.0	0.0	6.212646463140369E-4	0.0
240-244	0.0	0.0	0.0	6.212646463140369E-4	0.0
245-249	0.0	0.0	0.0	6.212646463140369E-4	0.0
250-254	0.0	0.0	0.0	6.212646463140369E-4	0.0
255-259	0.0	0.0	0.0	6.212646463140369E-4	0.0
260-264	0.0	0.0	0.0	6.212646463140369E-4	0.0
265-269	0.0	0.0	0.0	6.212646463140369E-4	0.0
270-274	0.0	0.0	0.0	6.212646463140369E-4	0.0
275-279	0.0	0.0	0.0	6.212646463140369E-4	0.0
280-284	0.0	0.0	0.0	6.212646463140369E-4	0.0
285-289	0.0	0.0	0.0	6.212646463140369E-4	0.0
290	0.0	0.0	0.0	6.212646463140369E-4	0.0
>>END_MODULE
