##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	ICMS1_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	194877
Sequences flagged as poor quality	0
Sequence length	35-301
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.213970863672984	34.0	34.0	34.0	20.0	34.0
2	33.13698897253139	34.0	34.0	34.0	34.0	34.0
3	33.610102782780935	34.0	34.0	34.0	34.0	34.0
4	33.47971797595407	34.0	34.0	34.0	34.0	34.0
5	33.26101592286417	34.0	34.0	34.0	34.0	34.0
6	33.14301841674492	34.0	34.0	34.0	34.0	34.0
7	33.55131698455949	34.0	34.0	34.0	34.0	34.0
8	33.48486481216357	34.0	34.0	34.0	34.0	34.0
9	33.38870672270201	34.0	34.0	34.0	34.0	34.0
10-14	33.25902594970161	34.0	34.0	34.0	31.2	34.0
15-19	33.427769310898675	34.0	34.0	34.0	34.0	34.0
20-24	33.63629366215613	34.0	34.0	34.0	34.0	34.0
25-29	33.54749303406764	34.0	34.0	34.0	34.0	34.0
30-34	33.472125494542716	34.0	34.0	34.0	34.0	34.0
35-39	33.53702750747048	34.0	34.0	34.0	34.0	34.0
40-44	33.43538875413539	34.0	34.0	34.0	34.0	34.0
45-49	33.50398719172388	34.0	34.0	34.0	34.0	34.0
50-54	33.451530732678556	34.0	34.0	34.0	34.0	34.0
55-59	33.40967599577162	34.0	34.0	34.0	34.0	34.0
60-64	33.31413118219978	34.0	34.0	34.0	34.0	34.0
65-69	33.38041606371296	34.0	34.0	34.0	34.0	34.0
70-74	33.32106899842976	34.0	34.0	34.0	34.0	34.0
75-79	33.405316715394505	34.0	34.0	34.0	34.0	34.0
80-84	33.55085850927442	34.0	34.0	34.0	34.0	34.0
85-89	33.520265790964615	34.0	34.0	34.0	34.0	34.0
90-94	33.249393942504085	34.0	34.0	34.0	34.0	34.0
95-99	33.28146623833814	34.0	34.0	34.0	34.0	34.0
100-104	33.19560313240894	34.0	34.0	34.0	34.0	34.0
105-109	33.387273305758825	34.0	34.0	34.0	34.0	34.0
110-114	33.29397637350795	34.0	34.0	34.0	34.0	34.0
115-119	33.27961039494212	34.0	34.0	34.0	34.0	34.0
120-124	33.112005296001314	34.0	34.0	34.0	34.0	34.0
125-129	33.07194966746038	34.0	34.0	34.0	34.0	34.0
130-134	33.20477871746449	34.0	34.0	34.0	34.0	34.0
135-139	33.05515231135561	34.0	34.0	34.0	34.0	34.0
140-144	33.237324492979724	34.0	34.0	34.0	34.0	34.0
145-149	33.17000369488464	34.0	34.0	34.0	34.0	34.0
150-154	33.08332418160451	34.0	34.0	34.0	34.0	34.0
155-159	32.96401471803267	34.0	34.0	34.0	31.2	34.0
160-164	33.24618116317618	34.0	34.0	34.0	34.0	34.0
165-169	33.2909364050408	34.0	34.0	34.0	34.0	34.0
170-174	32.80630201035119	34.0	34.0	34.0	31.2	34.0
175-179	33.214433878508274	34.0	34.0	34.0	34.0	34.0
180-184	33.10074975441943	34.0	34.0	34.0	34.0	34.0
185-189	33.03765314303679	34.0	34.0	34.0	34.0	34.0
190-194	33.19461345814345	34.0	34.0	34.0	34.0	34.0
195-199	32.722748080633906	34.0	34.0	34.0	31.2	34.0
200-204	32.991414172517146	34.0	34.0	34.0	34.0	34.0
205-209	32.4554686537751	34.0	34.0	34.0	28.4	34.0
210-214	32.43180502524941	34.0	34.0	34.0	31.2	34.0
215-219	33.03311265755225	34.0	34.0	34.0	34.0	34.0
220-224	33.045100997659816	34.0	34.0	34.0	34.0	34.0
225-229	33.17365376635165	34.0	34.0	34.0	34.0	34.0
230-234	32.66913997146582	34.0	34.0	34.0	31.2	34.0
235-239	32.88699231219272	34.0	34.0	34.0	34.0	34.0
240-244	32.75229248565593	34.0	34.0	34.0	34.0	34.0
245-249	32.733325464193705	34.0	34.0	34.0	34.0	34.0
250-254	32.720727313783655	34.0	34.0	34.0	31.2	34.0
255-259	32.43461771377544	34.0	34.0	34.0	28.4	34.0
260-264	32.646245907192046	34.0	34.0	34.0	34.0	34.0
265-269	32.72976717720464	34.0	34.0	34.0	34.0	34.0
270-274	32.41896879498346	34.0	34.0	34.0	31.2	34.0
275-279	32.33853184384604	34.0	34.0	34.0	28.4	34.0
280-284	32.12848292444709	34.0	34.0	34.0	25.6	34.0
285-289	32.16008309332769	34.0	34.0	34.0	22.8	34.0
290-294	31.785661369992475	34.0	34.0	34.0	25.6	34.0
295-299	31.77789321617953	34.0	34.0	34.0	20.0	34.0
300-301	31.27652903851729	34.0	34.0	34.0	20.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.19533412839730602
1101	2	0.03101033086052496
1101	3	0.01292033511661117
1101	4	0.036828232131000505
1101	5	0.14656301315154252
1101	6	0.16836953495116802
1101	7	-0.004441340635224833
1101	8	-0.10809588103711576
1101	9	-0.18187351447402023
1101	10-14	-0.06558290784763443
1101	15-19	-0.05694687912102836
1101	20-24	-0.05267859000856845
1101	25-29	-0.022893095654467288
1101	30-34	-0.02875102284730957
1101	35-39	0.0031013923569460644
1101	40-44	-0.08331275921010928
1101	45-49	-0.047548683499101685
1101	50-54	-0.11318559572627862
1101	55-59	-0.02581134490299064
1101	60-64	-0.04738046355512893
1101	65-69	-0.04002408231636423
1101	70-74	-0.11366991687351913
1101	75-79	-0.010141474735696931
1101	80-84	-0.08226557283398961
1101	85-89	-0.01027393185979264
1101	90-94	-0.24208283618236948
1101	95-99	-0.06359266744873793
1101	100-104	0.05886407671034277
1101	105-109	-0.04120501783914676
1101	110-114	-0.045570503684636776
1101	115-119	-0.20287327701407776
1101	120-124	-0.18583539073456024
1101	125-129	-0.166150436447829
1101	130-134	-0.08319603930721797
1101	135-139	-0.09329084378579466
1101	140-144	-0.05655926467879624
1101	145-149	-0.03622473485325628
1101	150-154	-0.18248279443943005
1101	155-159	-0.3313172649952847
1101	160-164	-0.09711028673670086
1101	165-169	-0.07585708775584266
1101	170-174	-0.22325797278350734
1101	175-179	-0.17345547078784307
1101	180-184	-0.2755465631284011
1101	185-189	-0.22353321040944252
1101	190-194	-0.19319797562353358
1101	195-199	-0.22318779066166172
1101	200-204	0.1039705063639147
1101	205-209	-0.30401581604318295
1101	210-214	-0.2202116441504316
1101	215-219	-0.2778528134822764
1101	220-224	-0.2755123772123014
1101	225-229	-0.25847841655799186
1101	230-234	-0.4558515132440064
1101	235-239	-0.15454611669130713
1101	240-244	-0.34118546257759164
1101	245-249	-0.2794532263579228
1101	250-254	-0.3390835102058105
1101	255-259	-0.3339991973233012
1101	260-264	-0.397782301451322
1101	265-269	-0.5577682678932021
1101	270-274	-0.4640259041752728
1101	275-279	-0.47952457435302165
1101	280-284	-0.42726440444752356
1101	285-289	-0.5350971114819032
1101	290-294	-0.5710698489654469
1101	295-299	-0.7094078255258545
1101	300-301	-0.843214108467162
1102	1	0.17209656257486472
1102	2	0.009729712200510221
1102	3	0.022719251705282772
1102	4	0.12211614916400748
1102	5	-0.15883655386208062
1102	6	0.07721077643088847
1102	7	0.03280210548255269
1102	8	0.04880732098222751
1102	9	0.2042004902718091
1102	10-14	-0.0077357388033192365
1102	15-19	-0.019396655537455842
1102	20-24	-0.04045901486463066
1102	25-29	0.03662173271086999
1102	30-34	0.06213121053045256
1102	35-39	0.07858330241300138
1102	40-44	-0.0060724328631778235
1102	45-49	0.014734232446713236
1102	50-54	0.04802289005039029
1102	55-59	-0.04561621741780897
1102	60-64	0.010323952338680442
1102	65-69	-0.07659252347087175
1102	70-74	-0.12565598189711125
1102	75-79	0.01851267503220555
1102	80-84	0.019040003992728316
1102	85-89	-0.05795442951050944
1102	90-94	-0.027240762763767634
1102	95-99	-0.10555878484666437
1102	100-104	-0.096769945808731
1102	105-109	-0.06994803380671044
1102	110-114	-0.14492575104465288
1102	115-119	-0.1864396477752024
1102	120-124	-0.06909829287774727
1102	125-129	-0.03168358637645241
1102	130-134	-0.07970520481578802
1102	135-139	0.04691023962553231
1102	140-144	-0.09334041748138588
1102	145-149	-0.12156606614455256
1102	150-154	-0.22686389808199436
1102	155-159	-0.34265810468632907
1102	160-164	-0.024733966742758184
1102	165-169	-0.036259632732544844
1102	170-174	-0.2276332150384377
1102	175-179	-0.13532194459595104
1102	180-184	-0.2390546981672088
1102	185-189	-0.09804940958317587
1102	190-194	-0.12292891902333025
1102	195-199	-0.2328661706497499
1102	200-204	0.16806997978441274
1102	205-209	-0.3562015464004489
1102	210-214	-0.02779174190325051
1102	215-219	-0.04636912610222055
1102	220-224	-0.012628669033773576
1102	225-229	-0.08698130470947518
1102	230-234	-0.2063166427144978
1102	235-239	0.04165390788871548
1102	240-244	-0.28838145036322516
1102	245-249	-0.2656425680862853
1102	250-254	-0.18956896545600443
1102	255-259	-0.18925601118642277
1102	260-264	-0.2515222449172896
1102	265-269	-0.44528445761308433
1102	270-274	-0.5102958872528838
1102	275-279	-0.4814011637817259
1102	280-284	-0.6650713000864599
1102	285-289	-0.5542326513440656
1102	290-294	-0.7203972074649307
1102	295-299	-0.9822392541565392
1102	300-301	-1.0252137725701367
1103	1	-0.03703602750683643
1103	2	0.1635984740791514
1103	3	-0.13265821954106372
1103	4	0.12555483090470432
1103	5	-0.03258990927090366
1103	6	-0.3606578286987272
1103	7	-0.13636927998289394
1103	8	-0.06404707876697557
1103	9	0.08414543217670456
1103	10-14	-0.0700924603974542
1103	15-19	-0.05850063892205526
1103	20-24	0.04664152004142608
1103	25-29	0.05613306762474224
1103	30-34	0.08558380374714147
1103	35-39	0.15886917344774787
1103	40-44	0.058556169915206624
1103	45-49	0.02849287813513257
1103	50-54	0.027146858935715557
1103	55-59	0.10025638481194932
1103	60-64	0.006722643484330604
1103	65-69	0.10670586789225212
1103	70-74	0.026546341907192073
1103	75-79	-0.003125548217589369
1103	80-84	0.06574925414457766
1103	85-89	0.06826968240829245
1103	90-94	0.015456692023938956
1103	95-99	0.02381304343477808
1103	100-104	0.06291028617982874
1103	105-109	0.10468899423177902
1103	110-114	0.01853409181445187
1103	115-119	0.019356147432198156
1103	120-124	0.20359798629225878
1103	125-129	0.10889481752659691
1103	130-134	0.16644072852669467
1103	135-139	0.13619938279617116
1103	140-144	0.15455424717367094
1103	145-149	0.191307927232792
1103	150-154	0.22392670481394106
1103	155-159	0.030878741131402876
1103	160-164	0.12553446596292872
1103	165-169	-0.054513056719059705
1103	170-174	0.057522652273512165
1103	175-179	0.10538871629712787
1103	180-184	0.016775385889459926
1103	185-189	-0.02049195126861747
1103	190-194	0.036215427235269715
1103	195-199	0.04175322312339347
1103	200-204	0.26709127805821
1103	205-209	-0.12975896552774913
1103	210-214	0.02634545268107047
1103	215-219	0.03606812530362902
1103	220-224	-0.003244300830310465
1103	225-229	-0.019262786771513163
1103	230-234	-0.12108925865978648
1103	235-239	-0.2144691009088291
1103	240-244	-0.10180994341727967
1103	245-249	-0.08909124257733225
1103	250-254	-0.20430925191485017
1103	255-259	-0.07428843864101253
1103	260-264	-0.0602423891263939
1103	265-269	-0.1078155624977768
1103	270-274	-0.1529061503953031
1103	275-279	-0.03283639704387298
1103	280-284	-0.3336215675394847
1103	285-289	-0.24141058108130764
1103	290-294	-0.18980763519383004
1103	295-299	-0.6690545052898287
1103	300-301	-0.005034878183526104
1104	1	-0.2009959916771109
1104	2	0.06124033255171213
1104	3	0.046490434896760746
1104	4	-0.1162666435666182
1104	5	-0.028305919708259353
1104	6	0.07264824288284899
1104	7	-0.054077735020477746
1104	8	-0.18603854973321177
1104	9	0.026185000662117375
1104	10-14	-0.16820847862388888
1104	15-19	-0.08001892376841369
1104	20-24	-0.04695391556242612
1104	25-29	-0.09039690082950358
1104	30-34	-0.13383727348150387
1104	35-39	-0.2117261063746625
1104	40-44	-0.1172295527159477
1104	45-49	-0.1792922992609789
1104	50-54	-0.09621451505657319
1104	55-59	-0.22782808767315998
1104	60-64	-0.10962467123312791
1104	65-69	-0.23443893095495838
1104	70-74	-0.29577713825015195
1104	75-79	-0.20678446475768197
1104	80-84	-0.2555363321778117
1104	85-89	-0.16803226096515544
1104	90-94	-0.22000013704488453
1104	95-99	-0.38026356208767
1104	100-104	-0.19278060450054824
1104	105-109	-0.16057758675509604
1104	110-114	-0.12556373892901007
1104	115-119	-0.1349424466403235
1104	120-124	-0.24514115768872813
1104	125-129	-0.329045751854558
1104	130-134	-0.28585120588932966
1104	135-139	-0.11112020282519808
1104	140-144	-0.29214610722910095
1104	145-149	-0.24506119688606276
1104	150-154	-0.2579140476104058
1104	155-159	-0.39847437644463213
1104	160-164	-0.3733575385547283
1104	165-169	-0.34714070013534837
1104	170-174	-0.36237940014694203
1104	175-179	-0.37872352422941447
1104	180-184	-0.3403486940264173
1104	185-189	-0.484513254380353
1104	190-194	-0.3685446693492267
1104	195-199	-0.5033036371017161
1104	200-204	-0.08518897274990422
1104	205-209	-0.6137105564456924
1104	210-214	-0.3205837057097014
1104	215-219	-0.4001130874548764
1104	220-224	-0.29660995881216223
1104	225-229	-0.19632299629755323
1104	230-234	-0.2696093349927011
1104	235-239	-0.3670609145942265
1104	240-244	-0.4097927685136682
1104	245-249	-0.48455216090891895
1104	250-254	-0.5391088780394
1104	255-259	-0.5470889994541928
1104	260-264	-0.6565046082329857
1104	265-269	-0.5858124079236475
1104	270-274	-0.8280374630724268
1104	275-279	-0.5002219035553566
1104	280-284	-0.7924619324364386
1104	285-289	-0.807553760716516
1104	290-294	-1.1282768622500328
1104	295-299	-0.9560597054945141
1104	300-301	-1.1806678731785034
1201	1	-0.27653081403126833
1201	2	-0.11196200464553385
1201	3	-0.3240589949402022
1201	4	-0.037881006020668906
1201	5	-0.1987516835382479
1201	6	-0.21688149041278848
1201	7	-0.16310610180965313
1201	8	-0.08400999446038071
1201	9	-0.15072104023883526
1201	10-14	-0.003937910345683804
1201	15-19	-0.047532353228412205
1201	20-24	-0.15896880577069794
1201	25-29	-0.14131293136385636
1201	30-34	-0.09209144368224287
1201	35-39	-0.07428901279252642
1201	40-44	-0.23360218489010975
1201	45-49	-0.04611352251986034
1201	50-54	-0.1591237601795541
1201	55-59	-0.22248881710488178
1201	60-64	-0.2456278754551633
1201	65-69	-0.14213801680926252
1201	70-74	-0.22261155536446608
1201	75-79	-0.20366977245724627
1201	80-84	-0.20568523426055663
1201	85-89	-0.0694180869947445
1201	90-94	-0.26250367111330775
1201	95-99	-0.20055712537104853
1201	100-104	-0.07805390579166271
1201	105-109	-0.1738440083930186
1201	110-114	-0.3577454371666917
1201	115-119	-0.1442747433135878
1201	120-124	-0.1762103311879244
1201	125-129	-0.13641546253215608
1201	130-134	-0.020471882545621156
1201	135-139	-0.24608250609539084
1201	140-144	-0.07856444668814788
1201	145-149	-0.07854060504740801
1201	150-154	-0.12897285450891616
1201	155-159	-0.1876422211341051
1201	160-164	-0.15831748577931393
1201	165-169	-0.031206292445226325
1201	170-174	-0.22300375223439062
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2404	255-259	0.5240074676883992
2404	260-264	0.44545351408482503
2404	265-269	0.4006373449593852
2404	270-274	0.5334023742306329
2404	275-279	0.5403175014796631
2404	280-284	0.7213121967977116
2404	285-289	0.5505746178855304
2404	290-294	0.7275056238918047
2404	295-299	0.777579955133131
2404	300-301	0.9380086269883385
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	24.0
16	52.0
17	84.0
18	151.0
19	243.0
20	532.0
21	765.0
22	806.0
23	733.0
24	717.0
25	751.0
26	883.0
27	1097.0
28	1537.0
29	2156.0
30	3229.0
31	5653.0
32	16267.0
33	153841.0
34	5355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.22732404195488412	0.07081426137646504	0.09749789609803157	99.60436380057061
2	0.0415672388576707	0.15857131860518822	0.23606086264850024	99.56380057988864
3	0.09390538647454548	0.16574557284851468	99.57152460269812	0.16882443797882768
4	0.10262883767709889	99.55356455610462	0.17292959148591164	0.17087701473236966
5	0.18627134038393448	0.2370726150340984	0.15137753557372086	99.42527850900824
6	99.49096096512159	0.16523242866012922	0.05285385140370593	0.29095275481457533
7	99.822965255007	0.06311673517141582	0.049774986273392954	0.06414302354818681
8	99.6141155703341	0.12315460521251866	0.09082652134423251	0.17190330310914065
9	30.739902605233045	21.004017918995057	25.764456554647293	22.491622921124605
10-14	59.7235179112979	11.227800099549972	8.844142715661674	20.204539273490457
15-19	35.41341461537277	24.418889863862848	19.88228472318437	20.28541079758001
20-24	59.712536625666445	39.74619888442453	0.424472872632481	0.11679161727653853
25-29	15.087465426910306	15.37503143008154	59.413065677324674	10.124437465683481
30-34	0.818055304684806	59.29294816006831	0.3971740094254422	39.49182252582144
35-39	56.21991032236943	5.075027273781557	19.807898158724687	18.89716424512433
40-44	38.71053487730058	46.502417452043524	0.26519618257074035	14.521851488085158
45-49	20.214600203208228	19.941192770713386	24.92389954534725	34.92030748073114
50-54	44.56469308373616	20.215113355296243	19.51127395137371	15.708919609593892
55-59	20.343401377300204	31.149871198825906	8.615926188203659	39.890801235670224
60-64	39.980602851073	0.15928240812011865	0.5176678263903856	59.34244691441649
65-69	31.950696347383438	20.251444523127766	42.02099818344161	5.776860946047189
70-74	40.0549072734177	54.029475455935625	5.788663444071554	0.12695382657512033
75-79	39.97473233932149	19.92512069361884	11.73827547702716	28.361871490032513
80-84	40.00119053605939	0.2856260218340208	39.604310972124004	20.108872469982582
85-89	33.906855062601934	6.135389308150926	49.16142460826967	10.79633102097747
90-94	19.927539950530107	59.30618989459423	9.108720864594131	11.657549290281526
95-99	11.391930865312574	8.692434801350672	59.497500846735704	20.418133486601047
100-104	27.89917173852801	16.456436730881734	20.917245697043096	34.727145833547155
105-109	57.939917687025954	36.865641004587765	4.8418913509796475	0.35254995740662814
110-114	47.72654028922439	46.07597015384931	5.879527470158981	0.3179620867673171
115-119	35.26726332211183	38.65998850480335	19.24685934805813	6.825888825026685
120-124	30.320531242302323	28.54749979472863	34.06365465144921	7.068314311519829
125-129	18.133178148019283	41.719395803633105	24.719829953023602	15.427596095324017
130-134	30.561211922161096	20.772436160604318	30.302672633221118	18.363679284013465
135-139	7.227912800722555	15.333052795796043	36.18246571968142	41.25656868379998
140-144	39.72360210197882	19.392294112817144	19.94806634370638	20.93603744149766
145-149	5.142766236965269	32.16766565399458	33.00240167501437	29.68716643402578
150-154	30.116440781472107	18.503564042429808	7.597440252895623	43.782554923202454
155-159	26.36693471823794	65.59244186941595	0.18915853702344726	7.851464875322662
160-164	26.833724206237203	13.59416615776212	4.891231275306241	54.68087836069444
165-169	33.72735455649263	26.95355599278671	25.689280549474347	13.629808901246316
170-174	7.263323842817348	30.35925058451862	20.777969139058982	41.59945643360505
175-179	57.2181783098499	1.5176987880579367	14.387193319538872	26.876929582553288
180-184	34.38502640864947	33.84012349353691	5.728716558110318	26.0461335397033
185-189	34.33481680981394	6.014443367874796	19.16055622156898	40.490183600742284
190-194	26.328700505707296	32.88996273141472	28.203830730211777	12.577506032666207
195-199	22.4781377016874	40.55702672742949	17.897216405961323	19.06761916492179
200-204	72.43493680874072	0.5509934923898865	20.251050605679726	6.763019093189679
205-209	20.851705875107772	45.15231760890093	6.979820996017572	27.016155519973722
210-214	42.468284271462004	18.89364453750462	37.712361949336945	0.9257092416964322
215-219	40.09771318306852	20.13969290142464	15.228476413351396	24.53411750215544
220-224	20.645010310188354	22.111120120622534	47.46504851279767	9.778821056391445
225-229	42.31102023974849	1.5466949134275783	36.803209784546596	19.339075062277335
230-234	20.236792675541686	52.84130682459688	20.720539480842064	6.201361019019369
235-239	49.336323606392476	25.435762160386748	2.904123087029263	22.323791146191507
240-244	33.94562082379628	32.3019286234822	7.679493364262474	26.07295718845905
245-249	50.69092827545575	13.081089514292255	16.367553519613168	19.86042869063883
250-254	26.704609605140263	15.371201001775688	45.087501411313085	12.836687981770966
255-259	13.785090375357962	39.595799932256966	15.278105658595667	31.3410040337894
260-264	38.97779876215012	28.275221447853266	30.99407761708767	1.7529021729089471
265-269	21.078081185053804	53.102944980231314	13.839621130941385	11.979352703773499
270-274	11.223173256447708	7.940890582708329	31.167931385942783	49.66800477490118
275-279	40.097080004269955	15.486816498468562	19.357596833660423	25.058506663601054
280-284	12.408094556241563	43.49453673908246	8.522481742084816	35.574886962591165
285-289	45.63015411271763	26.343071702342996	20.864546273847235	7.162227911092132
290-294	27.412851570519404	20.257852597002667	20.27232601108602	32.05696982139191
295-299	36.85241860942796	26.512170072917733	22.17479716545137	14.460614152202938
300-301	30.428408156510677	48.54292614561947	20.938939943216784	0.08972575465306903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.25
23	0.5
24	0.25
25	0.8333333333333333
26	1.0
27	0.5
28	1.0
29	2.0
30	1.5
31	1.25
32	0.5
33	2.5
34	2.25
35	1.75
36	1.0
37	1.0
38	2.25
39	2.5
40	2.75
41	4.25
42	6.583333333333334
43	9.666666666666668
44	9.666666666666666
45	15.333333333333332
46	25.25
47	25.833333333333332
48	44.666666666666664
49	81.75
50	1877.6666666666656
51	2417.8333333333335
52	10529.583333333341
53	2734.8333333333344
54	11416.166666666677
55	30618.083333333176
56	3621.2500000000127
57	141.83333333333331
58	37.333333333333336
59	22.16666666666666
60	12.66666666666667
61	8.75
62	5.25
63	2.9166666666666665
64	1.5
65	1.0
66	0.0
67	0.25
68	0.5833333333333333
69	0.5
70	0.25
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.3333333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.131441883854945E-4
2	0.0061577302606259324
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	1.0262883767709889E-4
35-39	1.0262936431371744E-4
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	1.0263568437474341E-4
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	1.0263726451162623E-4
180-184	2.0527473971163003E-4
185-189	0.0
190-194	1.02639898181221E-4
195-199	0.0
200-204	0.6188159461345815
205-209	0.0
210-214	0.0
215-219	0.0
220-224	1.02639898181221E-4
225-229	1.026407409840373E-4
230-234	0.0
235-239	0.0
240-244	0.0
245-249	1.0264095168690404E-4
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	1.0270093180555427E-4
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-39	2.0
40-49	1.0
50-59	0.0
60-69	0.0
70-79	1.0
80-89	7.0
90-99	0.0
100-109	0.0
110-119	2.0
120-129	0.0
130-139	0.0
140-149	1.0
150-159	0.0
160-169	1.0
170-179	1.0
180-189	5.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	2.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	1.0
270-279	4.0
280-289	5.0
290-299	1074.0
300-302	193770.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.0964300558814015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.622447701365395	2.3604632665732743
2	11.687335588124766	0.9575270555273326
3	6.000250532381311	0.7373881987099555
4	4.371790053864462	0.7163492869861503
5	3.4448202430164097	0.7055732590300549
6	2.392584241513216	0.5880632398897767
7	1.9040460979581613	0.545985416442166
8	1.5157209069272204	0.4967235743571586
9	1.377928097206564	0.5080127465016394
>10	8.73105348866341	6.562087881073703
>50	0.2755856194413128	0.7260990265654746
>100	0.2755856194413128	2.170086772682256
>500	0.0626330953275711	2.156745023784233
>1k	0.1252661906551422	16.5027170984775
>5k	0.2004259050482275	57.84109977062455
>10k+	0.012526619065514218	6.425078382774776
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12521	6.425078382774776	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9666	4.960051724934189	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9330	4.787635277636664	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9075	4.656783509598362	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9024	4.630613155990702	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8224	4.220097805282307	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8070	4.14107360027094	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7719	3.9609599901476313	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6709	3.442684359878282	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6278	3.2215192146841343	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6043	3.100930330413543	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6021	3.089641158269062	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5784	2.9680259856216997	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5257	2.6975989983425444	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5251	2.6945201332122313	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5162	2.6488503004459223	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5106	2.6201142258963346	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4956	2.5431425976385107	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4688	2.405619955151198	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4550	2.3348060571539997	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4351	2.2326903636652866	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4254	2.1829153773918932	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3265	1.6754157750786394	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2568	1.3177542757739498	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1425	0.7312304684493296	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1098	0.5634323188472729	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1005	0.5157099093274219	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	952	0.48851326734299066	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	949	0.4869738347778342	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	888	0.4556720392863191	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	873	0.44797487646053663	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	541	0.2776110059165525	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	329	0.16882443797882768	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	296	0.15189067976210635	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	266	0.1364963541105415	No Hit
CCTACGGGTGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	265	0.13598320992215604	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	256	0.1313649122266866	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	256	0.1313649122266866	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	237	0.12161517264736219	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	234	0.1200757400822057	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	233	0.11956259589382022	No Hit
CCTACGGGGGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	210	0.10776027956095383	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
