##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAMS2_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151673
Sequences flagged as poor quality	0
Sequence length	51-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.70974398871256	40.0	40.0	40.0	40.0	40.0
2	39.331205949641664	40.0	40.0	40.0	40.0	40.0
3	39.33749579687881	40.0	40.0	40.0	40.0	40.0
4	39.04486625833207	40.0	40.0	40.0	40.0	40.0
5	39.2144943397968	40.0	40.0	40.0	40.0	40.0
6	39.38897496588054	40.0	40.0	40.0	40.0	40.0
7	39.38201261925326	40.0	40.0	40.0	40.0	40.0
8	39.438476195499526	40.0	40.0	40.0	40.0	40.0
9	39.03603146242245	40.0	40.0	40.0	40.0	40.0
10-14	39.59523448471382	40.0	40.0	40.0	40.0	40.0
15-19	39.49221812715513	40.0	40.0	40.0	40.0	40.0
20-24	39.44112399701991	40.0	40.0	40.0	40.0	40.0
25-29	39.61226058692055	40.0	40.0	40.0	40.0	40.0
30-34	39.51065252220237	40.0	40.0	40.0	40.0	40.0
35-39	39.6055619655443	40.0	40.0	40.0	40.0	40.0
40-44	39.5652172766412	40.0	40.0	40.0	40.0	40.0
45-49	39.51119052171448	40.0	40.0	40.0	40.0	40.0
50-54	39.48853487003831	40.0	40.0	40.0	40.0	40.0
55-59	39.43685320955747	40.0	40.0	40.0	40.0	40.0
60-64	39.414388944564585	40.0	40.0	40.0	40.0	40.0
65-69	39.42505406403292	40.0	40.0	40.0	40.0	40.0
70-74	39.42876929815784	40.0	40.0	40.0	40.0	40.0
75-79	39.46145011241437	40.0	40.0	40.0	40.0	40.0
80-84	39.44331085045922	40.0	40.0	40.0	40.0	40.0
85-89	39.576346170329195	40.0	40.0	40.0	40.0	40.0
90-94	39.46886616426344	40.0	40.0	40.0	40.0	40.0
95-99	39.38241061244404	40.0	40.0	40.0	40.0	40.0
100-104	39.29782947358642	40.0	40.0	40.0	40.0	40.0
105-109	39.37010008835203	40.0	40.0	40.0	40.0	40.0
110-114	39.3718249310986	40.0	40.0	40.0	40.0	40.0
115-119	39.27707462450384	40.0	40.0	40.0	40.0	40.0
120-124	39.243284585866306	40.0	40.0	40.0	40.0	40.0
125-129	39.31417720517453	40.0	40.0	40.0	40.0	40.0
130-134	39.320907784210036	40.0	40.0	40.0	40.0	40.0
135-139	39.074835493782395	40.0	40.0	40.0	40.0	40.0
140-144	39.40789503181354	40.0	40.0	40.0	40.0	40.0
145-149	39.07411202320905	40.0	40.0	40.0	40.0	40.0
150-154	39.191417927669534	40.0	40.0	40.0	40.0	40.0
155-159	39.21762305080276	40.0	40.0	40.0	40.0	40.0
160-164	39.20536445455444	40.0	40.0	40.0	40.0	40.0
165-169	39.23983516302377	40.0	40.0	40.0	40.0	40.0
170-174	39.09466257870966	40.0	40.0	40.0	40.0	40.0
175-179	39.351359905053904	40.0	40.0	40.0	40.0	40.0
180-184	39.26013780371213	40.0	40.0	40.0	40.0	40.0
185-189	39.18167540302641	40.0	40.0	40.0	40.0	40.0
190-194	39.268060528137674	40.0	40.0	40.0	40.0	40.0
195-199	39.233188936142156	40.0	40.0	40.0	40.0	40.0
200-204	39.361703754986316	40.0	40.0	40.0	40.0	40.0
205-209	39.21997560412752	40.0	40.0	40.0	40.0	40.0
210-214	39.28169584281146	40.0	40.0	40.0	40.0	40.0
215-219	39.3443127946461	40.0	40.0	40.0	40.0	40.0
220-224	39.23417532060792	40.0	40.0	40.0	40.0	40.0
225-229	39.30236571502817	40.0	40.0	40.0	40.0	40.0
230-234	39.157879744957505	40.0	40.0	40.0	40.0	40.0
235-239	39.235220656595956	40.0	40.0	40.0	40.0	40.0
240-244	39.256984986252235	40.0	40.0	40.0	40.0	40.0
245-249	39.0502818786636	40.0	40.0	40.0	40.0	40.0
250-254	39.249035678256114	40.0	40.0	40.0	40.0	40.0
255-259	38.98198218394973	40.0	40.0	40.0	40.0	40.0
260-264	38.972761619664915	40.0	40.0	40.0	40.0	40.0
265-269	38.889386196846914	40.0	40.0	40.0	40.0	40.0
270-274	38.32393034465024	40.0	40.0	40.0	36.8	40.0
275-279	38.84541444405615	40.0	40.0	40.0	40.0	40.0
280-284	38.577944230883354	40.0	40.0	40.0	36.8	40.0
285-289	38.96403689808191	40.0	40.0	40.0	40.0	40.0
290-294	38.94588572315299	40.0	40.0	40.0	40.0	40.0
295-299	38.93559371906413	40.0	40.0	40.0	40.0	40.0
300-301	38.401412175468835	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.06906738945988877
1101	2	-0.27628766309400277
1101	3	0.07267248290177974
1101	4	-0.0787404386522681
1101	5	0.05055767576020287
1101	6	0.18753935017946333
1101	7	0.11131393452311755
1101	8	-0.20089246494665502
1101	9	0.07402970295707689
1101	10-14	0.10826279165523545
1101	15-19	0.07983051709597788
1101	20-24	0.042940421022855446
1101	25-29	0.04793391099161681
1101	30-34	-0.03620245229372898
1101	35-39	0.07077606835751737
1101	40-44	0.054381380863617323
1101	45-49	0.02815243939312495
1101	50-54	0.07096698851607641
1101	55-59	-0.11261023214229482
1101	60-64	-0.015262082272258226
1101	65-69	0.06511616076556948
1101	70-74	0.0866246281294849
1101	75-79	0.10191034490870265
1101	80-84	0.15659819999788738
1101	85-89	0.028677010375197654
1101	90-94	0.02892552047565289
1101	95-99	0.19885703931557686
1101	100-104	0.006568135970546507
1101	105-109	-0.006194565453142786
1101	110-114	0.06501838457227649
1101	115-119	0.02453372380408325
1101	120-124	0.1511805301872613
1101	125-129	0.14698537898752306
1101	130-134	0.002939114077491922
1101	135-139	5.918404334295246E-4
1101	140-144	2.5939758429416315E-4
1101	145-149	0.0999932835545323
1101	150-154	0.15627731152656565
1101	155-159	0.036028746863216554
1101	160-164	-0.10910649304355502
1101	165-169	0.06324073544656272
1101	170-174	0.04171629969691537
1101	175-179	0.004979279197911524
1101	180-184	-0.032137200176933334
1101	185-189	0.03173573205651081
1101	190-194	0.04798900132466599
1101	195-199	0.04382826326060041
1101	200-204	-0.02351602194732294
1101	205-209	-0.012487290303091925
1101	210-214	-0.05451964719278379
1101	215-219	0.030803213828008325
1101	220-224	-0.18136233282432812
1101	225-229	0.12036153644777414
1101	230-234	-0.09158226783626588
1101	235-239	0.044319855972695166
1101	240-244	-0.15971952742567908
1101	245-249	-0.12478004516174934
1101	250-254	-0.10421554492646123
1101	255-259	-0.15257724997061217
1101	260-264	0.016393838611016065
1101	265-269	-0.03555588022540945
1101	270-274	0.03576741377623449
1101	275-279	0.03719870818586202
1101	280-284	-0.050005303291605685
1101	285-289	-0.04670058073546102
1101	290-294	-0.09843282217644855
1101	295-299	0.004472819721499377
1101	300-301	-0.09133024757318253
1102	1	-0.15315483276233266
1102	2	-0.1651765519828885
1102	3	-0.2447878345585366
1102	4	0.09586273595090944
1102	5	0.10611323131576
1102	6	-0.1061114434713275
1102	7	-0.11884479563561712
1102	8	-0.1215273855815795
1102	9	-0.1640655351381639
1102	10-14	-0.08221339882095435
1102	15-19	-0.2011218638564003
1102	20-24	-0.13959926151682112
1102	25-29	-0.15206608900838603
1102	30-34	-0.252075468166737
1102	35-39	-0.1800175824361432
1102	40-44	-0.20911068262844879
1102	45-49	-0.2512126399719605
1102	50-54	-0.20998539243630887
1102	55-59	-0.35705467658674195
1102	60-64	-0.24383351084368599
1102	65-69	-0.27774098209157927
1102	70-74	-0.16258172107687585
1102	75-79	-0.23142298842463305
1102	80-84	-0.16403672063702146
1102	85-89	-0.1983071166089303
1102	90-94	-0.2520268604767324
1102	95-99	-0.3154286749701285
1102	100-104	-0.2791461497437453
1102	105-109	-0.2934961527547273
1102	110-114	-0.3143466947928033
1102	115-119	-0.30562500635465284
1102	120-124	-0.2519940729873511
1102	125-129	-0.3387289067267645
1102	130-134	-0.39388628274789994
1102	135-139	-0.4375033976618141
1102	140-144	-0.24101044368554625
1102	145-149	-0.31270512914387893
1102	150-154	-0.48340522815597353
1102	155-159	-0.3750823642478949
1102	160-164	-0.46942395336100873
1102	165-169	-0.2986640264581979
1102	170-174	-0.3138392558586389
1102	175-179	-0.2585127842941475
1102	180-184	-0.34642291446265006
1102	185-189	-0.27143887111809306
1102	190-194	-0.18534433200866118
1102	195-199	-0.23871141927908468
1102	200-204	-0.15208745051874928
1102	205-209	-0.2458206236364191
1102	210-214	-0.1021386948118348
1102	215-219	-0.2818951988704015
1102	220-224	-0.18453693599892773
1102	225-229	-0.09392417783793405
1102	230-234	-0.1455505218045161
1102	235-239	-0.31917220751937236
1102	240-244	-0.3279734956796432
1102	245-249	-0.21366893405063792
1102	250-254	-0.2375488782597941
1102	255-259	-0.36210105949442806
1102	260-264	-0.3248760026588329
1102	265-269	-0.41968286435238866
1102	270-274	-0.2483595703507575
1102	275-279	-0.462801291814138
1102	280-284	-0.5055608588471614
1102	285-289	-0.48479581883069756
1102	290-294	-0.557162980906611
1102	295-299	-0.37078357839772025
1102	300-301	-0.7441701800896396
1103	1	0.19630247321671135
1103	2	0.09092483455327738
1103	3	0.16182487640200094
1103	4	-0.16955542077159436
1103	5	0.1404629395785122
1103	6	0.05475184782674347
1103	7	0.3108559384129421
1103	8	0.15785024162213546
1103	9	0.2591103228203053
1103	10-14	0.0699354185452492
1103	15-19	0.12582461961181934
1103	20-24	0.07807436920028721
1103	25-29	0.14730024514055629
1103	30-34	0.10499837586362304
1103	35-39	0.1605621277087863
1103	40-44	0.15386691947959008
1103	45-49	0.08692024163917722
1103	50-54	0.19441889892450348
1103	55-59	0.021262658252332756
1103	60-64	0.03298435727852933
1103	65-69	0.07500385760978645
1103	70-74	0.09006273591541714
1103	75-79	0.01663523354662999
1103	80-84	-0.018858227632442492
1103	85-89	0.04761169887510874
1103	90-94	0.06089113939778912
1103	95-99	-0.024023944434972577
1103	100-104	0.006085044183102184
1103	105-109	0.05635013622199381
1103	110-114	0.09882853577372686
1103	115-119	0.1331352671882371
1103	120-124	0.11237633920914902
1103	125-129	0.13791329165457
1103	130-134	0.19210405541635112
1103	135-139	0.13696175071638095
1103	140-144	0.1685008179996288
1103	145-149	0.13465668778315631
1103	150-154	0.255116636452847
1103	155-159	0.1399562203558986
1103	160-164	0.11884381751546158
1103	165-169	0.113231324567586
1103	170-174	0.25842374683914215
1103	175-179	0.12907112937671172
1103	180-184	0.14466812010664398
1103	185-189	0.0915387309899387
1103	190-194	-0.033258253835626306
1103	195-199	0.09083246678654433
1103	200-204	0.11803614569180354
1103	205-209	0.16910503041966507
1103	210-214	0.10489436874296842
1103	215-219	0.12949196469066493
1103	220-224	0.1842754110742817
1103	225-229	0.11133964046935318
1103	230-234	0.1538158123444191
1103	235-239	0.05171053292858829
1103	240-244	0.04605247834632564
1103	245-249	0.10379138340967842
1103	250-254	0.18195673689800174
1103	255-259	0.2091957596284857
1103	260-264	0.11828856224487083
1103	265-269	0.2138135908831984
1103	270-274	0.09112220391363479
1103	275-279	0.07786625320122909
1103	280-284	0.12475471929451487
1103	285-289	0.07754811742628931
1103	290-294	0.09711896902751249
1103	295-299	0.2768213092288363
1103	300-301	-0.06712386267246018
1104	1	-0.03433397108432956
1104	2	0.0488824049332095
1104	3	-0.07381277786919327
1104	4	0.04710990148378613
1104	5	0.04556901362868615
1104	6	-0.16688241852801866
1104	7	-0.08211010175806166
1104	8	-0.14102851936842598
1104	9	-0.6924102063399786
1104	10-14	-0.06692995210893571
1104	15-19	-0.022437056600168148
1104	20-24	-0.07388497580253528
1104	25-29	-0.05319987585646402
1104	30-34	-0.09035211215767447
1104	35-39	-0.0557998953613108
1104	40-44	-0.12434877786653686
1104	45-49	-0.11094053112842062
1104	50-54	-0.2744751883546783
1104	55-59	-0.2199118194438867
1104	60-64	-0.09299450857611191
1104	65-69	-0.2577863335654982
1104	70-74	-0.230472877539448
1104	75-79	-0.3233050745924331
1104	80-84	-0.22408207211094577
1104	85-89	-0.211050880781265
1104	90-94	-0.18744636161051176
1104	95-99	-0.10513389038963794
1104	100-104	-0.3376949025781997
1104	105-109	-0.1432013681742319
1104	110-114	-0.21366642268396419
1104	115-119	-0.2364186571482918
1104	120-124	-0.30138182808939007
1104	125-129	-0.4094772060464891
1104	130-134	-0.30159603331479445
1104	135-139	-0.2961882049180531
1104	140-144	-0.2689469516220555
1104	145-149	-0.33179809966541995
1104	150-154	-0.34431225763443507
1104	155-159	-0.46868780642477503
1104	160-164	-0.3886983297782365
1104	165-169	-0.3536300128527614
1104	170-174	-0.3280342671965073
1104	175-179	-0.3271295643395007
1104	180-184	-0.384472801083966
1104	185-189	-0.47239125207048005
1104	190-194	-0.3802196154553741
1104	195-199	-0.32859804059427233
1104	200-204	-0.21081760924890602
1104	205-209	-0.3226913719357398
1104	210-214	-0.3317532072834908
1104	215-219	-0.39686118526496017
1104	220-224	-0.3101151672914497
1104	225-229	-0.35809651343884497
1104	230-234	-0.371582267836267
1104	235-239	-0.3895123435737844
1104	240-244	-0.3671571691490385
1104	245-249	-0.4333514737331825
1104	250-254	-0.2871180392575212
1104	255-259	-0.4411486785420422
1104	260-264	-0.4034247554932904
1104	265-269	-0.5891613224022691
1104	270-274	-0.4851849671761528
1104	275-279	-0.5816675049660702
1104	280-284	-0.5949032624752704
1104	285-289	-0.38320851724339633
1104	290-294	-0.7136255659406245
1104	295-299	-0.7866836428635366
1104	300-301	-0.5667846828739087
1201	1	0.16198260432632594
1201	2	0.33579131483863733
1201	3	0.40871584333538635
1201	4	0.09954062987270618
1201	5	0.22748373073503814
1201	6	0.0801061329901458
1201	7	-0.518380219561287
1201	8	-0.20321534531831986
1201	9	0.18397937388235164
1201	10-14	0.016315443610331215
1201	15-19	-0.002360733387952507
1201	20-24	0.07166670828573274
1201	25-29	0.04290100351968107
1201	30-34	-0.01971000165493564
1201	35-39	-0.02635904585076787
1201	40-44	0.027474683225221952
1201	45-49	-0.03204887690575475
1201	50-54	-0.019276913922951167
1201	55-59	-0.10293156392705072
1201	60-64	-0.02679905478483846
1201	65-69	-0.07069491163087349
1201	70-74	-0.05654223211054443
1201	75-79	0.01023399957382054
1201	80-84	0.040918757489187385
1201	85-89	0.0793158024774101
1201	90-94	-0.11629321742601206
1201	95-99	-0.14125136176843256
1201	100-104	-0.031101240723224066
1201	105-109	-0.09783219611515648
1201	110-114	-0.05743612568711143
1201	115-119	-0.17407254797291927
1201	120-124	-0.21262125068768967
1201	125-129	-0.1498787325107358
1201	130-134	-0.20499739385900995
1201	135-139	-0.0783086628573173
1201	140-144	-0.1305961966859428
1201	145-149	-0.1317914628643564
1201	150-154	-0.09916210001040326
1201	155-159	-0.12072696355103574
1201	160-164	-0.14824702730603434
1201	165-169	-0.26800200555228315
1201	170-174	-0.11829144323765917
1201	175-179	-0.01604278816561333
1201	180-184	-0.11014726599187696
1201	185-189	-0.16005675729695668
1201	190-194	-0.3124446030113788
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2404	230-234	0.1681905952838747
2404	235-239	0.09324062372191122
2404	240-244	0.1767485605068444
2404	245-249	0.13056962608749956
2404	250-254	0.10490313741397017
2404	255-259	0.27641258864264984
2404	260-264	0.22588444098864358
2404	265-269	0.2214475069035018
2404	270-274	0.259776180462822
2404	275-279	0.2340373878697335
2404	280-284	0.32160258672632835
2404	285-289	0.26688114205128244
2404	290-294	0.23474775828446326
2404	295-299	0.15902551946248167
2404	300-301	0.050362362937548255
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	4.0
17	12.0
18	26.0
19	35.0
20	45.0
21	67.0
22	85.0
23	146.0
24	327.0
25	307.0
26	313.0
27	339.0
28	339.0
29	393.0
30	480.0
31	552.0
32	737.0
33	828.0
34	1093.0
35	1342.0
36	1924.0
37	2984.0
38	5991.0
39	116584.0
40	16720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.07054650465145412	0.00593381814825315	0.08768864596862988	99.83583103123166
2	0.013186349491006909	0.009889762118255182	0.1549396065193312	99.82198428187141
3	0.010549010041338933	0.12790674675123456	99.61034594159804	0.25119830160938333
4	0.08175482782037673	99.40134368015401	0.10614941354097301	0.4107520784846347
5	0.1971346251475213	0.1509827062166635	0.08702933284104619	99.56485333579477
6	99.76132864781471	0.0712058177790378	0.012526949424089984	0.1549385849821656
7	99.89780646522452	0.06197543399286623	0.02637252510334733	0.013845575679257349
8	99.7336374964562	0.10944597917889143	0.08702933284104619	0.06988719152387042
9	29.175265208705568	21.165929334818987	26.572956294132773	23.085849162342672
10-14	59.82013937879517	10.429410639995252	9.588918264951573	20.161531716258
15-19	39.54256855208244	20.1867174777317	19.96241915172773	20.30829481845813
20-24	61.297132647208144	38.44309798052389	0.2285179300205046	0.031251442247466586
25-29	19.606917513334608	19.790074700177353	59.7691085427202	0.8338992437678426
30-34	4.275513769754669	57.4938189394289	0.33743645869732913	37.893230832119094
35-39	64.35674114707298	1.4429727110296493	19.840709948375782	14.359576193521589
40-44	34.850632610945915	49.10999320907479	0.08689747021552946	15.952476709763769
45-49	20.08650188233898	19.910069689397584	21.395897753720174	38.60753067454326
50-54	38.34290220237828	20.168468356800577	17.646585667530807	23.842043773290328
55-59	23.43794503929532	27.632390948889707	9.303101429400284	39.62656258241468
60-64	39.97441848198744	0.08584313518645499	0.334010232607205	59.605728150218894
65-69	32.63713803470647	21.464344110976317	42.051927844295584	3.8465900100216257
70-74	40.02803432688605	55.388762006234536	4.456877772444604	0.1263258944348037
75-79	36.92861522637815	22.254353172326944	12.077588991962868	28.739442609332038
80-84	39.99604406907055	0.17129180924501058	39.293207007272315	20.539457114412116
85-89	33.99779786511594	6.040179071806739	55.38316487660793	4.578858186469398
90-94	15.279783214985066	61.19020775230598	10.812746009454676	12.71726302325428
95-99	6.375114557166499	12.218815726144088	61.23728333036639	20.168786386323028
100-104	25.21207645697482	22.903993617596445	10.434174869945341	41.449755055483394
105-109	63.8963248190102	33.01425500771432	0.8265530837498187	2.262867089525668
110-114	40.34444107446626	51.86014004457162	5.761475874619229	2.033943006342885
115-119	38.282937507417614	31.837194888768742	19.62061371698337	10.259253886830273
120-124	23.339970725146046	28.45133385201693	33.29157490802157	14.91712051481545
125-129	21.364841164136987	39.806021125367586	22.04132765418749	16.78781005630794
130-134	23.509422019437448	38.01379346722403	26.46117125789564	12.015613255442881
135-139	12.51750557145306	14.866878535729827	21.056400247913178	51.55921564490393
140-144	39.738634490488906	19.53766524906867	18.691458147891733	22.032242112550687
145-149	0.5998747238980648	32.13899053835757	29.24801371443642	38.01312102330795
150-154	37.547621402432995	11.414894669172188	13.30986054791811	37.72762338047671
155-159	30.360465499620876	55.91349355487423	0.06791283420696931	13.658128111297927
160-164	29.291398806580293	11.196254903900043	0.24118946362047936	59.27115682589918
165-169	32.9995714238618	27.369927142056504	28.338904823129923	11.29159661095177
170-174	9.63847954373125	31.89806481389905	20.47314805657205	37.990307585797645
175-179	63.57828108001187	4.3920482642666405	13.508851745623579	18.520818910097915
180-184	32.70787591072429	33.26040945504896	5.575314014439718	28.456400619787033
185-189	32.78488774601919	8.970691985626216	28.317673820591434	29.926746447763165
190-194	27.606369300761546	28.35050934625655	22.69211749579666	21.351003857185244
195-199	21.844459829228892	33.623710150661	26.866976560181982	17.664853459928132
200-204	65.94850492862558	2.1263969933735534	25.552368707348432	6.372729370652425
205-209	23.99643952131342	44.48013714436422	12.484884449279662	19.038538885042694
210-214	47.982065736986115	27.171727161836944	24.34141034516863	0.5047967560083078
215-219	41.691359245705996	18.4904889064715	19.296607655029177	20.52154419279333
220-224	28.274816206771504	24.84897636237761	27.82949263178716	19.04671479906373
225-229	41.63206152572078	7.419192765030982	30.40192953606473	20.54681617318351
230-234	20.438346048094104	49.265005505700216	19.59343535912331	10.703213087082375
235-239	45.47774312446839	26.33333553121765	8.406643764712088	19.782277579601875
240-244	31.535200216271818	37.0070090530855	12.236896763175768	19.220893967466917
245-249	39.30305088321981	14.341854530828623	17.97377044856621	28.381324137385356
250-254	37.03325179182519	1.6225661178549529	44.314358998028496	17.02982309229136
255-259	11.132591767164927	38.06027917526589	13.982500445071574	36.82462861249761
260-264	41.85532206697833	24.92348065751907	22.046142383341795	11.175054892160805
265-269	21.138064499113156	43.22139508509109	16.062006712338704	19.57853370345705
270-274	14.572764257126092	8.534165012758718	36.57842161135691	40.31464911875828
275-279	32.487587448322245	27.80200578922729	20.04984801629951	19.660558746150954
280-284	20.872340285241425	32.66957226973315	15.19335887274909	31.26472857227633
285-289	40.62402331515683	18.258484382932988	29.758474492453562	11.359017809456617
290-294	31.233903159827037	21.551600222075404	22.1011181575654	25.113378460532164
295-299	38.85216469033168	24.311360815140894	24.375868178082534	12.460606316444894
300-301	36.242217212992664	42.415816807840876	20.317501299199957	1.024464679966502
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.5
45	1.6666666666666665
46	3.5833333333333335
47	0.5
48	4.5
49	125.91666666666666
50	998.666666666667
51	533.6666666666666
52	289.91666666666663
53	1531.583333333333
54	15894.333333333347
55	25147.66666666662
56	4623.250000000002
57	1384.666666666666
58	536.5
59	29.25
60	9.916666666666668
61	3.0
62	1.0
63	1.3333333333333333
64	16.25
65	2.25
66	0.25
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.593131275836833E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	1.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	5.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	1.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	4.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	4.0
295-299	365.0
300-302	151292.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.7864352917130932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.016019502002436	2.1210103314367093
2	14.57426432178304	1.1036901755750859
3	7.504788438098554	0.8524918739657026
4	4.283475535434442	0.6487641175423444
5	3.134250391781299	0.5933818148253149
6	2.0895002611875326	0.474705451860252
7	1.7064252133031519	0.45228880552240674
8	1.2885251610656452	0.39031337152954054
9	1.3407626675953335	0.45690399741549254
>10	6.198850774856346	4.211692259004569
>50	0.5049625631203204	1.288297851298517
>100	0.6790875848859481	5.7445952806366325
>500	0.15671251958906496	4.876279891608922
>1k	0.383075047884381	36.73099365081458
>5k	0.1393000174125022	40.05459112696393
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9346	6.161940490397104	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8604	5.672730149730011	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8103	5.342414272810586	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7951	5.242198677417866	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7640	5.037152294739341	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	7195	4.743757952964602	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6893	4.544645388434329	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	5020	3.3097519004700904	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4161	2.743401923875706	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3887	2.562750126917777	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3643	2.4018777237873583	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3534	2.330012592880737	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3367	2.219907300574262	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3306	2.179689199791657	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3278	2.161228432219314	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3092	2.038596190488749	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3068	2.0227726754267406	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	3060	2.0174981704060713	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2809	1.8520105753825664	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2728	1.7986062120482882	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2638	1.7392680305657566	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	2470	1.6285034251316979	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	2232	1.4715869007667812	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1845	1.2164327203918956	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1263	0.8327124801381921	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1145	0.7549135310833174	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1073	0.7074429858972923	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1050	0.6922787839628675	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1036	0.6830484001766959	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	1026	0.676455268900859	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	965	0.6362371681182544	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	949	0.6256881580769155	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	877	0.5782176128908902	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	876	0.5775582997633066	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	803	0.5294284414496977	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	787	0.5188794314083587	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	745	0.49118828004984405	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	733	0.48327652251883985	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	661	0.4358059773328146	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	455	0.29998747305057594	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	439	0.289438463009237	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	400	0.26372525103347333	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	381	0.25119830160938333	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	380	0.2505389884817996	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	357	0.23537478654737493	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	329	0.21691401897503182	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	324	0.2136174533371134	No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	275	0.18131111008551293	No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	272	0.17933317070276186	No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	245	0.16153171625800242	No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	234	0.1542792718545819	No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	232	0.15296064559941452	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC	231	0.15230133247183084	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	228	0.1503233930890798	No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	211	0.13911506992015718	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	209	0.1377964436649898	No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	207	0.13647781740982246	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	202	0.13318125177190404	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	191	0.1259288073684835	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	191	0.1259288073684835	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	184	0.12131361547539773	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	179	0.11801704983747932	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	179	0.11801704983747932	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	174	0.11472048419956091	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	174	0.11472048419956091	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC	173	0.11406117107197722	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	168	0.11076460543405879	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	161	0.10614941354097301	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT	152	0.10021559539271988	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
