##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAMS1_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151730
Sequences flagged as poor quality	0
Sequence length	51-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.754906742239505	40.0	40.0	40.0	40.0	40.0
2	39.072813550385554	40.0	40.0	40.0	40.0	40.0
3	39.18668687800699	40.0	40.0	40.0	40.0	40.0
4	39.077651090753314	40.0	40.0	40.0	40.0	40.0
5	39.19433203717129	40.0	40.0	40.0	40.0	40.0
6	39.355407631977855	40.0	40.0	40.0	40.0	40.0
7	39.33194490212878	40.0	40.0	40.0	40.0	40.0
8	39.43958347063863	40.0	40.0	40.0	40.0	40.0
9	38.977077703815986	40.0	40.0	40.0	40.0	40.0
10-14	39.57473933961642	40.0	40.0	40.0	40.0	40.0
15-19	39.45421999604561	40.0	40.0	40.0	40.0	40.0
20-24	39.421498714822384	40.0	40.0	40.0	40.0	40.0
25-29	39.59412113622883	40.0	40.0	40.0	40.0	40.0
30-34	39.487921966651285	40.0	40.0	40.0	40.0	40.0
35-39	39.6167191722138	40.0	40.0	40.0	40.0	40.0
40-44	39.54403216239373	40.0	40.0	40.0	40.0	40.0
45-49	39.44008963290055	40.0	40.0	40.0	40.0	40.0
50-54	39.53756155979276	40.0	40.0	40.0	40.0	40.0
55-59	39.40749508983299	40.0	40.0	40.0	40.0	40.0
60-64	39.43560892661772	40.0	40.0	40.0	40.0	40.0
65-69	39.34212989204224	40.0	40.0	40.0	40.0	40.0
70-74	39.3855502682467	40.0	40.0	40.0	40.0	40.0
75-79	39.41075359529678	40.0	40.0	40.0	40.0	40.0
80-84	39.449270395317875	40.0	40.0	40.0	40.0	40.0
85-89	39.56779457706655	40.0	40.0	40.0	40.0	40.0
90-94	39.43643673464008	40.0	40.0	40.0	40.0	40.0
95-99	39.281794814336365	40.0	40.0	40.0	36.8	40.0
100-104	39.27366436866456	40.0	40.0	40.0	40.0	40.0
105-109	39.32355957449613	40.0	40.0	40.0	40.0	40.0
110-114	39.327666978632536	40.0	40.0	40.0	40.0	40.0
115-119	39.25593767712851	40.0	40.0	40.0	40.0	40.0
120-124	39.11940735272795	40.0	40.0	40.0	40.0	40.0
125-129	39.3071879572387	40.0	40.0	40.0	40.0	40.0
130-134	39.3015936622596	40.0	40.0	40.0	40.0	40.0
135-139	38.90092930677669	40.0	40.0	40.0	40.0	40.0
140-144	39.37434288576626	40.0	40.0	40.0	40.0	40.0
145-149	38.919000290000255	40.0	40.0	40.0	36.8	40.0
150-154	39.166750151591046	40.0	40.0	40.0	40.0	40.0
155-159	39.236376578524165	40.0	40.0	40.0	40.0	40.0
160-164	39.282787166170145	40.0	40.0	40.0	40.0	40.0
165-169	39.247813134375576	40.0	40.0	40.0	40.0	40.0
170-174	39.02404365822151	40.0	40.0	40.0	40.0	40.0
175-179	39.292536447760405	40.0	40.0	40.0	40.0	40.0
180-184	39.29340644855132	40.0	40.0	40.0	40.0	40.0
185-189	39.206192662890864	40.0	40.0	40.0	40.0	40.0
190-194	39.26434884625271	40.0	40.0	40.0	40.0	40.0
195-199	39.19898762877085	40.0	40.0	40.0	40.0	40.0
200-204	39.41984801249646	40.0	40.0	40.0	40.0	40.0
205-209	39.24263559249421	40.0	40.0	40.0	40.0	40.0
210-214	39.311909202955384	40.0	40.0	40.0	40.0	40.0
215-219	39.29866928547419	40.0	40.0	40.0	40.0	40.0
220-224	39.24706471662174	40.0	40.0	40.0	40.0	40.0
225-229	39.342095792991174	40.0	40.0	40.0	40.0	40.0
230-234	39.15195982151684	40.0	40.0	40.0	40.0	40.0
235-239	39.25191038932792	40.0	40.0	40.0	40.0	40.0
240-244	39.232664790440474	40.0	40.0	40.0	40.0	40.0
245-249	39.11880993652907	40.0	40.0	40.0	40.0	40.0
250-254	39.26895197168525	40.0	40.0	40.0	40.0	40.0
255-259	38.97911325244031	40.0	40.0	40.0	40.0	40.0
260-264	39.047548492977334	40.0	40.0	40.0	40.0	40.0
265-269	38.86532167173071	40.0	40.0	40.0	40.0	40.0
270-274	37.76919254167134	40.0	36.8	40.0	33.6	40.0
275-279	38.939082406754416	40.0	40.0	40.0	40.0	40.0
280-284	38.30692033695108	40.0	40.0	40.0	36.8	40.0
285-289	38.98284494008779	40.0	40.0	40.0	40.0	40.0
290-294	38.95304173187122	40.0	40.0	40.0	40.0	40.0
295-299	38.97375869851111	40.0	40.0	40.0	40.0	40.0
300-301	38.40188593522383	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.05075228473655358
1101	2	0.0026887675102074127
1101	3	0.3119870109822003
1101	4	0.21786835688077133
1101	5	0.13238268947284837
1101	6	0.13446937697170114
1101	7	0.21188335633530642
1101	8	0.2602915792780678
1101	9	0.3567508148644478
1101	10-14	0.0728273646343851
1101	15-19	0.08249438972148937
1101	20-24	0.004611165845993526
1101	25-29	0.11255957752836565
1101	30-34	0.08618215626290748
1101	35-39	0.08690708422085436
1101	40-44	0.22525723097097483
1101	45-49	0.034351183846752065
1101	50-54	0.0709330636282246
1101	55-59	0.004786587820191812
1101	60-64	-0.057232761339179206
1101	65-69	0.13044858409636362
1101	70-74	0.2090488071876564
1101	75-79	0.19277515204439055
1101	80-84	0.07673695892173527
1101	85-89	0.13223194068340405
1101	90-94	0.1358759108400065
1101	95-99	0.19597509306033345
1101	100-104	0.022867197693955177
1101	105-109	0.20421630668017343
1101	110-114	0.052880361475779125
1101	115-119	0.06229673826928206
1101	120-124	0.1683534824366717
1101	125-129	0.12070428986339721
1101	130-134	0.08993883007534009
1101	135-139	0.15473986372867188
1101	140-144	0.04417028818659219
1101	145-149	0.160067591285582
1101	150-154	0.11159208177589619
1101	155-159	0.10368244789685832
1101	160-164	0.18615988003050887
1101	165-169	0.16335471729472317
1101	170-174	0.3328507323682004
1101	175-179	0.23611621596142385
1101	180-184	0.11726936074627758
1101	185-189	0.1106549511974606
1101	190-194	0.1343136632227271
1101	195-199	0.20816096415220642
1101	200-204	0.1258975153017161
1101	205-209	0.20059012148495015
1101	210-214	0.13679746779282453
1101	215-219	0.04444909822326082
1101	220-224	0.15965714897404837
1101	225-229	0.11784163594699493
1101	230-234	0.13785611454160573
1101	235-239	0.09566781755028586
1101	240-244	-0.019698448282390757
1101	245-249	0.05774993004720841
1101	250-254	0.1308613008918016
1101	255-259	0.06391333435350788
1101	260-264	-0.015154138511270787
1101	265-269	0.13536933603388235
1101	270-274	0.2657869784273572
1101	275-279	0.04902987153806748
1101	280-284	-0.08450500610531009
1101	285-289	-0.008611340276104329
1101	290-294	0.06135853322926721
1101	295-299	0.052998284202367074
1101	300-301	0.04289582407072601
1102	1	0.058739733032091124
1102	2	-0.17089559991241288
1102	3	-0.018588750915775165
1102	4	0.262417398862425
1102	5	0.21491965519337697
1102	6	0.21001732543364682
1102	7	-0.12848653859171577
1102	8	-0.11659236499996695
1102	9	0.02503398892425679
1102	10-14	0.07980687302595157
1102	15-19	0.07419314881434502
1102	20-24	-0.023287849986267872
1102	25-29	0.03707819113555644
1102	30-34	0.007600879222067647
1102	35-39	0.024566952047599955
1102	40-44	0.08098870056637253
1102	45-49	-0.021190108408283947
1102	50-54	0.06554153602873214
1102	55-59	0.010501416898307525
1102	60-64	0.0395675049091011
1102	65-69	0.1101756796129365
1102	70-74	-0.03692656484677315
1102	75-79	0.04282031916272899
1102	80-84	0.05798547316126701
1102	85-89	0.09069720954661875
1102	90-94	-0.03248808858946717
1102	95-99	0.14673817963856806
1102	100-104	0.0027179084817632315
1102	105-109	-0.08939848911690973
1102	110-114	-0.012835418489039796
1102	115-119	-0.06847870983607152
1102	120-124	-0.08966011524316997
1102	125-129	0.028068907369224405
1102	130-134	0.08540310050751287
1102	135-139	-0.16778188780463665
1102	140-144	-0.1765685507807504
1102	145-149	-0.021076325416750308
1102	150-154	-0.015959860410191595
1102	155-159	-0.09644116737437969
1102	160-164	-0.016369478596402587
1102	165-169	0.08615222597156702
1102	170-174	0.15823940730758324
1102	175-179	-0.06841475917288875
1102	180-184	-0.04978241503463465
1102	185-189	-0.03299550285093744
1102	190-194	0.09527025524478461
1102	195-199	0.004576121286234525
1102	200-204	-0.03291007277416469
1102	205-209	-0.03043731159640828
1102	210-214	0.11041987068352199
1102	215-219	0.1283077964593673
1102	220-224	-0.031052687948879054
1102	225-229	0.16794385626744912
1102	230-234	-0.05612477263568394
1102	235-239	0.044053687373896366
1102	240-244	-0.02782852958319637
1102	245-249	-0.11285816199862353
1102	250-254	-0.025607105897528015
1102	255-259	-0.07309993051597985
1102	260-264	0.04953468381555837
1102	265-269	-0.18446140613319528
1102	270-274	-0.004616672977590497
1102	275-279	-0.010999605949699287
1102	280-284	0.008520239936622431
1102	285-289	-0.04302393001604088
1102	290-294	-0.0197425935714719
1102	295-299	0.02350593884031582
1102	300-301	-0.23077654068690379
1103	1	0.08425807433990684
1103	2	0.04048132725399256
1103	3	0.12913409154391076
1103	4	-0.22228931742323965
1103	5	0.07995598338760601
1103	6	-0.040204187945761305
1103	7	-0.03591166706947746
1103	8	0.22989000253502923
1103	9	0.3122570971999963
1103	10-14	-0.02841924783174221
1103	15-19	-0.021245447676882634
1103	20-24	-0.022607360884727257
1103	25-29	0.0900712799181207
1103	30-34	-0.024268693700129518
1103	35-39	-0.07447256593928131
1103	40-44	-0.041527691423702606
1103	45-49	-0.1562671950643164
1103	50-54	-0.08824900091968857
1103	55-59	-0.009374535609225632
1103	60-64	-0.16318989363776382
1103	65-69	-0.027935254287470457
1103	70-74	-0.12612734457386665
1103	75-79	-0.1101763138339038
1103	80-84	-0.1109743000090262
1103	85-89	-0.13088705414290303
1103	90-94	-0.014880433087071765
1103	95-99	-0.164330400903701
1103	100-104	-0.255556059265885
1103	105-109	-0.11446317102369363
1103	110-114	-0.06282301373979493
1103	115-119	-0.033717058232312525
1103	120-124	-0.160062383540172
1103	125-129	-0.005218351181198955
1103	130-134	-0.09220455499487912
1103	135-139	-0.1836883205531663
1103	140-144	-0.17044414886687065
1103	145-149	-0.1251060968149389
1103	150-154	-0.20397332842365756
1103	155-159	-0.060318537567539465
1103	160-164	-0.1370428792698064
1103	165-169	-0.09327006713493802
1103	170-174	-0.11574251720066542
1103	175-179	-0.17953295871213726
1103	180-184	-0.11987279249343175
1103	185-189	-0.2908725186226917
1103	190-194	-0.2482633261835332
1103	195-199	-0.13413024057803113
1103	200-204	-0.10886966873376025
1103	205-209	-0.1499198563421018
1103	210-214	-0.1217686815050314
1103	215-219	-0.07450630951262838
1103	220-224	-0.1583325036497527
1103	225-229	-0.1633409213331234
1103	230-234	-0.2974432202699191
1103	235-239	-0.21073277372835975
1103	240-244	-0.25409115584582764
1103	245-249	-0.32191008473476046
1103	250-254	-0.13380487304266353
1103	255-259	-0.24058531063293742
1103	260-264	-0.17896788573964528
1103	265-269	-0.08329535970398183
1103	270-274	-0.37199572667242364
1103	275-279	-0.21474938443630975
1103	280-284	-0.44545105192940326
1103	285-289	-0.2512819488003757
1103	290-294	-0.377840168511149
1103	295-299	-0.3382330775039648
1103	300-301	-0.610579057877672
1104	1	0.024562760546231743
1104	2	-0.012904994984793916
1104	3	-0.08552065261903152
1104	4	-0.17317522568620092
1104	5	-0.045346218963778995
1104	6	-0.21399123878199333
1104	7	-0.11145397539866764
1104	8	-0.11935656146562934
1104	9	0.03987756416472621
1104	10-14	-0.035049484625915284
1104	15-19	0.09984744849798233
1104	20-24	-0.11486104526955643
1104	25-29	0.020023258722559945
1104	30-34	-0.043672568513848375
1104	35-39	-0.009041203130870201
1104	40-44	-0.004637477812174495
1104	45-49	-0.0012878938555047625
1104	50-54	0.07747711267527535
1104	55-59	-0.14436708404440424
1104	60-64	-0.04617051956921614
1104	65-69	-0.028021361258822708
1104	70-74	-0.14201076898188347
1104	75-79	-0.1138888490218477
1104	80-84	0.0021934429947805256
1104	85-89	0.024701619478548764
1104	90-94	-0.08099733985971369
1104	95-99	0.04228990047069914
1104	100-104	-0.10768058319369089
1104	105-109	0.08221177515944333
1104	110-114	-0.042548133792777776
1104	115-119	0.0713864357236389
1104	120-124	0.05897056893540764
1104	125-129	-0.016640772111813362
1104	130-134	-0.026561236564674573
1104	135-139	0.01900749001148938
1104	140-144	0.1606570268244667
1104	145-149	-0.050967994480117795
1104	150-154	-9.628962328989132E-4
1104	155-159	-0.0198681318712417
1104	160-164	-0.0018582460523859368
1104	165-169	0.06507402956981423
1104	170-174	-0.029844189662988185
1104	175-179	-6.424139199552314E-4
1104	180-184	0.027065442313656263
1104	185-189	0.0214639609269085
1104	190-194	0.10146013796615705
1104	195-199	0.11645098147860722
1104	200-204	0.07127936256281231
1104	205-209	0.06480443575923545
1104	210-214	0.11436644020386666
1104	215-219	0.031014472073728427
1104	220-224	0.08589998517290809
1104	225-229	0.08064318199524934
1104	230-234	0.07958608921840238
1104	235-239	0.019898125427133095
1104	240-244	0.08364688025286426
1104	245-249	0.08051415768948544
1104	250-254	0.04728139951901511
1104	255-259	0.057702485359783395
1104	260-264	0.017872650773021803
1104	265-269	-0.09731092519495377
1104	270-274	-0.1224577236917952
1104	275-279	0.037474493689209964
1104	280-284	0.014042241662252763
1104	285-289	-0.02849671945776322
1104	290-294	-0.18282379383990843
1104	295-299	-0.05655789332662664
1104	300-301	0.1586625702918667
1201	1	0.11631958008562293
1201	2	0.07111969473446322
1201	3	0.10346852903624182
1201	4	0.1964404907802546
1201	5	-0.05108724239246243
1201	6	0.20941753536017416
1201	7	0.06794471047111017
1201	8	0.26967244231747145
1201	9	-0.15880166859561484
1201	10-14	0.08076653714352489
1201	15-19	0.12007708943512085
1201	20-24	0.1883180879354569
1201	25-29	0.16393379167536182
1201	30-34	0.05882295149677219
1201	35-39	0.10271959862129876
1201	40-44	0.12261413166549318
1201	45-49	0.1353551007685141
1201	50-54	0.12923924183169788
1201	55-59	0.1076074297937879
1201	60-64	0.16240451195663752
1201	65-69	0.08888313200459663
1201	70-74	0.19135353711754277
1201	75-79	0.12643105540504251
1201	80-84	0.13379893844842172
1201	85-89	0.19668011552952436
1201	90-94	0.05838010754188616
1201	95-99	0.11398964162686553
1201	100-104	0.08087055505546203
1201	105-109	0.11225093358514471
1201	110-114	0.18989362634527396
1201	115-119	0.1444467662473059
1201	120-124	0.07420253283000733
1201	125-129	0.12457513019123212
1201	130-134	0.0794651787801186
1201	135-139	0.06307731147562379
1201	140-144	0.10485295169338826
1201	145-149	-0.020716451372670974
1201	150-154	0.0870240952053436
1201	155-159	0.054585973126442866
1201	160-164	0.04744818522125627
1201	165-169	0.010278781677072857
1201	170-174	0.13790652381680246
1201	175-179	0.09394841371660334
1201	180-184	0.025791134223126733
1201	185-189	-0.10521022769724198
1201	190-194	0.07847613318750746
1201	195-199	0.11175860741608545
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2404	220-224	0.0916463547012043
2404	225-229	0.18755407514326805
2404	230-234	0.12121780862366194
2404	235-239	0.14083875551758496
2404	240-244	0.205575275913624
2404	245-249	0.124488787710618
2404	250-254	0.12211243961883156
2404	255-259	0.24531555852374254
2404	260-264	0.19447820305528296
2404	265-269	0.2597819247686459
2404	270-274	0.0785750978518891
2404	275-279	0.19113269214343376
2404	280-284	0.32951723559703083
2404	285-289	0.19880026872370848
2404	290-294	0.24652843989662188
2404	295-299	0.27973989199898597
2404	300-301	0.17923609615152714
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	8.0
18	17.0
19	40.0
20	43.0
21	68.0
22	130.0
23	201.0
24	318.0
25	337.0
26	318.0
27	327.0
28	381.0
29	433.0
30	506.0
31	543.0
32	659.0
33	805.0
34	1039.0
35	1417.0
36	1963.0
37	2999.0
38	6233.0
39	119858.0
40	13084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0645884136294734	0.005931589006788374	0.10808673301258816	99.82139326435116
2	0.012522325989098984	0.013181395777998931	0.17135814511398612	99.80293813311891
3	0.008567850787583207	0.14104000527252356	99.59599288209319	0.25439926184670136
4	0.06920187174586437	99.40420483754036	0.09754168588940883	0.4290516048243591
5	0.2155144005799776	0.13774467804653004	0.10017794767020365	99.54656297370329
6	99.75680485072168	0.08501944243063336	0.013181308903974164	0.1449943979437158
7	99.89652672510381	0.06854280630066566	0.028998879588743164	0.005931589006788374
8	99.6961708297634	0.13379028537533777	0.0968826204442101	0.07315626441705661
9	28.687800698609372	21.876359322480724	26.581427535754298	22.854412443155606
10-14	59.81598892770052	10.394384762406906	9.60785606010677	20.181770249785806
15-19	39.918144071706315	20.124563369142557	19.92223027746655	20.03506228168457
20-24	59.90680814604891	39.81862518948132	0.24359058854544255	0.030976075924339288
25-29	19.68997561457853	19.878072892638237	59.60983325644237	0.8221182363408688
30-34	0.5367428985698279	59.54181770249786	0.2611217293877282	39.66031766954459
35-39	60.12429974296447	0.4140249126738285	19.854610162789164	19.60706518157253
40-44	39.508337177881764	45.73017860673565	0.08132867593752059	14.680155539445067
45-49	20.108877611546827	19.930929941343177	20.415738482831344	39.54445396427865
50-54	40.05269970657942	20.064114900650903	19.662869510886615	20.220315881883057
55-59	20.116921292329597	25.85001911340179	14.050459380725783	39.98260021354284
60-64	39.97165943872507	0.07922175500573402	0.37317269288058735	59.57594611338861
65-69	26.25364143258242	20.10835321566508	48.12952295585463	5.508482395897869
70-74	39.97548211908308	54.210880139198295	5.6856438580071975	0.12799388371142717
75-79	40.1281257002755	19.66070416408526	0.7599224918603271	39.45124764377892
80-84	40.085944399773275	0.10149875433346954	39.724767014223005	20.087789831670246
85-89	39.459420270751224	0.5041983575656117	54.08367715487128	5.952704216811885
90-94	20.012918023278807	59.678367583670564	14.18768042392207	6.121033969128561
95-99	5.985921990957384	13.818330411399495	59.73610323873297	20.45964435891014
100-104	28.6364894612657	20.132739280017926	6.5863464402936875	44.644424818422685
105-109	68.49636845365988	30.918497818435863	0.2210563779444525	0.3640773499597959
110-114	48.32092060688346	45.757483885425046	5.727034259125001	0.19456124856649487
115-119	39.69919460079353	34.56441216403253	19.876751512595074	5.85964172257886
120-124	20.574720219342762	33.93749258531827	39.3283946060662	6.159392589272768
125-129	17.114667229084006	47.885925945454304	20.24030159629859	14.759105229163097
130-134	21.164203893861302	22.02549332349103	42.15256449125397	14.657738291393695
135-139	5.929504501535663	14.700578674716263	28.976839829693002	50.39307699405507
140-144	39.789090717355194	19.98286362078511	20.01529092299175	20.212754738867943
145-149	0.4626822388020353	25.85457804961641	33.946244496585905	39.73649521499565
150-154	39.29569481426801	14.622472384065805	6.458305871187156	39.623526930479024
155-159	26.10846009860009	67.71993883630803	0.051540955946323595	6.1200601091455535
160-164	25.878964435422212	14.322058474598615	0.2503229548390498	59.548654135140126
165-169	34.05855368959426	25.848250771137067	25.6148005589096	14.478394980359072
170-174	6.120982837257125	25.710764282512983	20.056022778202525	48.11223010202737
175-179	56.417837652579685	0.4849595317813925	14.591758719780653	28.505444095858266
180-184	34.64184967440879	33.9942263583876	5.48350953046321	25.880414436740395
185-189	34.57396815520875	5.715795981371364	17.16070164523073	42.54953421818915
190-194	25.573182707961216	34.30633457023655	28.605023628586306	11.515459093215927
195-199	28.56903699505019	39.3585679165321	12.184968659992222	19.887426428425485
200-204	73.7592850128194	0.26667018184454566	19.965990654020814	6.008054151315226
205-209	20.52859487355246	45.23770291913553	5.97879029547267	28.254911911839336
210-214	36.940740691918826	28.409403979620755	34.15619253508038	0.49366279338004126
215-219	40.097018909460004	20.164510324736526	19.489991629482674	20.248479136320796
220-224	17.33422091574778	28.274948425749557	39.8683126487085	14.522518009794164
225-229	43.43744850813654	0.40666214087514746	36.14982566914706	20.006063681841248
230-234	20.328625191961667	53.87238586107578	19.998813627465843	5.800175319496715
235-239	45.421722481100424	25.42066792773673	0.776546733191408	28.381062857971433
240-244	34.61703235501539	31.32273946600054	6.086486557740092	27.973741621243974
245-249	52.95769263724024	6.058277255261232	20.26087013834422	20.723159969154313
250-254	25.776184230472637	0.8374471899448337	61.75187677543945	11.634491804143076
255-259	14.465044851472749	39.75455270459983	14.669364565688788	31.111037878238633
260-264	40.09451434522123	33.52491052773805	25.807689012213046	0.5728861148276794
265-269	20.474285375322133	53.269313156212306	14.722224053044034	11.534177415421524
270-274	6.278283450762244	6.436730093657521	30.97710960104928	56.30787685453096
275-279	47.19047211035901	12.286601240418396	19.942263203337664	20.580663445884937
280-284	11.70969047377378	44.95703331483461	0.8338924692629028	42.49938374212871
285-289	48.41104124649029	20.33469107973794	25.284665374830283	5.969602298941485
290-294	25.714816777530825	20.184527528849728	20.294996216645178	33.80565947697426
295-299	31.35354963261474	25.671737444813093	28.48725887869693	14.487454043875243
300-301	27.838598375244857	37.67264266324613	34.47715830259159	0.01160065891742651
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.25
42	0.25
43	0.0
44	0.0
45	2.25
46	4.916666666666666
47	0.5
48	2.25
49	10.75
50	338.08333333333326
51	259.58333333333337
52	58.58333333333335
53	346.91666666666663
54	16041.916666666686
55	31211.916666666526
56	2801.8333333333344
57	152.08333333333331
58	41.91666666666667
59	15.75
60	4.833333333333334
61	1.0833333333333333
62	0.0
63	0.3333333333333333
64	0.3333333333333333
65	0.3333333333333333
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	6.590654451987082E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	4.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	2.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	1.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	1.0
285-289	0.0
290-294	6.0
295-299	492.0
300-302	151224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.429315231002439
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.71676614215952	1.3049495814934424
2	12.018448182311449	0.5839319844460555
3	5.751492132392838	0.4191656231463784
4	3.825284861638633	0.37171291109207144
5	2.7129679869777537	0.3295327225993541
6	2.3060227889310907	0.3361233770513412
7	2.4688008681497555	0.4198246885915771
8	1.87194791101465	0.36380412574968696
9	1.7362995116657625	0.3796216964344559
>10	12.289744981009223	5.8162525538786
>50	0.3798155181768855	0.5931589006788374
>100	0.48833423765599565	2.0879193303895076
>500	0.0	0.0
>1k	0.16277807921866522	17.033546431160616
>5k	0.08138903960933261	12.354840835694985
>10k+	0.18990775908844276	57.605615237593085
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	14268	9.40354577209517	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	13204	8.702300138403743	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12883	8.49074013049496	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12570	8.284452646147763	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12543	8.266657879127397	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	11235	7.404600276807487	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10702	7.053318394516575	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8349	5.502537401964015	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5349	3.5253410663678904	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	5048	3.3269623673630786	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4857	3.2010808673301256	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4842	3.1911948856521453	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4530	2.9855664667501483	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	4393	2.895274500757925	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3994	2.6323073881236407	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	3229	2.128122322546629	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	266	0.1753114084228564	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	241	0.15883477229288867	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	221	0.14565346338891452	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	221	0.14565346338891452	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	220	0.1449943979437158	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	214	0.14104000527252356	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	213	0.14038093982732486	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	183	0.1206089764713636	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	180	0.11863178013576747	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	167	0.11006392934818428	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	159	0.1047914057865946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
