FastQCFastQC Report
Mon 14 Oct 2024
IAFS2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameIAFS2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127785
Sequences flagged as poor quality0
Sequence length51-301
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG1303610.201510349415033No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG127639.98787025081191No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG126999.937786125132057No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG112638.814023555190358No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG109898.599600892123489No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG108238.469695191141371No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG105288.238838674335797No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG103068.065109363383808No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG96857.579136831396487No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8790.6878741636342294No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8390.6565715850843213No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG8240.6448331181281057No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7370.5767500097820558No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG7080.5540556403333724No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6870.5376217865946707No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6820.5337089642759322No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6740.5274484485659506No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6550.5125797237547444No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG3260.2551160151817506No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2770.21677035645811324No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2650.20737958289314082No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2620.2050318895018977No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2470.1932934225456822No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2320.18155495558946666No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2320.18155495558946666No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2260.17685956880698048No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG2120.16590366631451264No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1690.1322533943733615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)