##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAFS2_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127785
Sequences flagged as poor quality	0
Sequence length	51-301
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.40942207614352	40.0	40.0	40.0	40.0	40.0
2	39.12096098916148	40.0	40.0	40.0	40.0	40.0
3	39.075071409007315	40.0	40.0	40.0	40.0	40.0
4	39.36339946003052	40.0	40.0	40.0	40.0	40.0
5	38.66215909535548	40.0	40.0	40.0	40.0	40.0
6	37.9060140079039	40.0	40.0	40.0	24.0	40.0
7	38.69903353288727	40.0	40.0	40.0	40.0	40.0
8	38.85492037406581	40.0	40.0	40.0	40.0	40.0
9	39.30721133153344	40.0	40.0	40.0	40.0	40.0
10-14	39.16720741871111	40.0	40.0	40.0	40.0	40.0
15-19	39.02224517744649	40.0	40.0	40.0	40.0	40.0
20-24	39.04407559572719	40.0	40.0	40.0	40.0	40.0
25-29	39.1902774191024	40.0	40.0	40.0	40.0	40.0
30-34	39.182752279219	40.0	40.0	40.0	40.0	40.0
35-39	39.32899792620417	40.0	40.0	40.0	40.0	40.0
40-44	39.29482803145909	40.0	40.0	40.0	40.0	40.0
45-49	39.160120514927414	40.0	40.0	40.0	40.0	40.0
50-54	39.269407233482966	40.0	40.0	40.0	40.0	40.0
55-59	39.02343329368309	40.0	40.0	40.0	40.0	40.0
60-64	38.94131346647468	40.0	40.0	40.0	36.8	40.0
65-69	39.07540224128216	40.0	40.0	40.0	40.0	40.0
70-74	38.96008890001878	40.0	40.0	40.0	40.0	40.0
75-79	38.88403556000752	40.0	40.0	40.0	36.8	40.0
80-84	38.87275402241282	40.0	40.0	40.0	40.0	40.0
85-89	38.89289425906217	40.0	40.0	40.0	40.0	40.0
90-94	39.091973956050836	40.0	40.0	40.0	40.0	40.0
95-99	37.396606773931005	40.0	36.8	40.0	30.4	40.0
100-104	38.30634821260877	40.0	36.8	40.0	36.8	40.0
105-109	38.789513554122586	40.0	40.0	40.0	40.0	40.0
110-114	39.128685907468856	40.0	40.0	40.0	40.0	40.0
115-119	39.08434232767796	40.0	40.0	40.0	40.0	40.0
120-124	38.75429787766856	40.0	40.0	40.0	40.0	40.0
125-129	38.93420146497214	40.0	40.0	40.0	40.0	40.0
130-134	39.123013835848	40.0	40.0	40.0	40.0	40.0
135-139	38.151499405246355	40.0	40.0	40.0	33.6	40.0
140-144	38.009171727289804	40.0	36.8	40.0	36.8	40.0
145-149	38.503806423339384	40.0	40.0	40.0	36.8	40.0
150-154	38.91574218994553	40.0	40.0	40.0	40.0	40.0
155-159	39.101646528516866	40.0	40.0	40.0	40.0	40.0
160-164	38.36518499968697	40.0	40.0	40.0	36.8	40.0
165-169	38.91118136855944	40.0	40.0	40.0	40.0	40.0
170-174	38.75304576472798	40.0	40.0	40.0	36.8	40.0
175-179	38.94054967758092	40.0	40.0	40.0	40.0	40.0
180-184	38.75513053277406	40.0	40.0	40.0	40.0	40.0
185-189	38.98900018781695	40.0	40.0	40.0	40.0	40.0
190-194	37.947110749389594	40.0	36.8	40.0	36.8	40.0
195-199	38.82912414699806	40.0	40.0	40.0	36.8	40.0
200-204	38.493125899956176	40.0	40.0	40.0	40.0	40.0
205-209	38.61526325674576	40.0	40.0	40.0	36.8	40.0
210-214	37.55420709948037	40.0	36.8	40.0	36.8	40.0
215-219	38.48470544043072	40.0	40.0	40.0	36.8	40.0
220-224	38.423721279659425	40.0	40.0	40.0	36.8	40.0
225-229	38.17230326175421	40.0	40.0	40.0	33.6	40.0
230-234	38.714054967758095	40.0	40.0	40.0	36.8	40.0
235-239	38.12394039942403	40.0	40.0	40.0	33.6	40.0
240-244	38.50236649345771	40.0	40.0	40.0	36.8	40.0
245-249	37.070303011331625	40.0	36.8	40.0	31.2	40.0
250-254	38.38387278532524	40.0	40.0	40.0	36.8	40.0
255-259	38.570932198084265	40.0	40.0	40.0	40.0	40.0
260-264	38.08779815939398	40.0	40.0	40.0	33.6	40.0
265-269	37.75973830839543	40.0	40.0	40.0	33.6	40.0
270-274	38.48027358881855	40.0	40.0	40.0	40.0	40.0
275-279	37.87818209442615	40.0	40.0	40.0	30.4	40.0
280-284	38.35633153598736	40.0	40.0	40.0	36.8	40.0
285-289	38.443461860526995	40.0	40.0	40.0	40.0	40.0
290-294	37.96305867069439	40.0	40.0	40.0	33.6	40.0
295-299	38.267461527456376	40.0	40.0	40.0	36.8	40.0
300-301	34.829284805683756	40.0	32.0	40.0	18.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.3950861592582484
1101	2	0.03504324672388037
1101	3	-0.2968028133476608
1101	4	-0.2520346835639984
1101	5	8.866938846026073E-4
1101	6	0.010054991913477807
1101	7	-0.5270618339704569
1101	8	-0.46335939774309054
1101	9	-0.35346776479715203
1101	10-14	-0.4163136650677899
1101	15-19	-0.2847691461691113
1101	20-24	-0.24054689975386623
1101	25-29	-0.24629194171773605
1101	30-34	-0.2505717580313984
1101	35-39	-0.2050133607045339
1101	40-44	-0.19175731421986342
1101	45-49	-0.2700868527799827
1101	50-54	-0.24297400345162146
1101	55-59	-0.2814704297655837
1101	60-64	-0.480726652416017
1101	65-69	-0.29078392608917625
1101	70-74	-0.3910035042404303
1101	75-79	-0.352054648232766
1101	80-84	-0.17456985817870674
1101	85-89	-0.2982709512519577
1101	90-94	-0.2068718433934862
1101	95-99	-0.08392434625305611
1101	100-104	-0.22250085456428792
1101	105-109	-0.3510853297494734
1101	110-114	-0.21134450665203275
1101	115-119	-0.2793235232538436
1101	120-124	-0.2589101442546067
1101	125-129	-0.3923152658340854
1101	130-134	-0.30154080028829355
1101	135-139	-0.1695244772782516
1101	140-144	-0.32410901901234723
1101	145-149	-0.3544789148781149
1101	150-154	-0.1946466799039186
1101	155-159	-0.2733901435109516
1101	160-164	-0.36453725092484746
1101	165-169	-0.19623161347343654
1101	170-174	-0.04297032888251806
1101	175-179	0.01224278446986915
1101	180-184	-0.3123612659541237
1101	185-189	-0.20101275483364844
1101	190-194	-0.11882347391749448
1101	195-199	-0.2858096942198358
1101	200-204	-0.18805873160445685
1101	205-209	-0.3712178727217008
1101	210-214	-0.09281744687175575
1101	215-219	-0.3147440918367721
1101	220-224	-0.4115889509282695
1101	225-229	-0.5324209432252118
1101	230-234	-0.4472667620265369
1101	235-239	-0.23268856128693471
1101	240-244	-0.4484912773442602
1101	245-249	0.40498131667706616
1101	250-254	-0.21963647536259145
1101	255-259	-0.34764452688889236
1101	260-264	-0.3692124287840315
1101	265-269	-0.44261908887377643
1101	270-274	-0.40853788889786813
1101	275-279	-0.21637771358442848
1101	280-284	-0.6812766842362095
1101	285-289	-0.5743052487220481
1101	290-294	-0.07200737105535637
1101	295-299	-0.8873480791079444
1101	300-301	-0.9265184907569619
1102	1	-0.5045279081846843
1102	2	0.20136972042863732
1102	3	-0.047603103348215825
1102	4	-0.23162310584942958
1102	5	-0.09900734437316316
1102	6	-0.40022887401703144
1102	7	-0.0856809851611331
1102	8	-0.17779807935593794
1102	9	-0.5025057437163483
1102	10-14	-0.47685685842007786
1102	15-19	-0.3522857375471773
1102	20-24	-0.49701000833676545
1102	25-29	-0.32975410222189083
1102	30-34	-0.3574615020501426
1102	35-39	-0.2807570448270056
1102	40-44	-0.3029613742276709
1102	45-49	-0.1366419807706194
1102	50-54	-0.25883034163688734
1102	55-59	-0.33893069411223564
1102	60-64	-0.34788855080427794
1102	65-69	-0.3060424169708966
1102	70-74	-0.231431812756405
1102	75-79	-0.5273399083967334
1102	80-84	-0.5331087015226288
1102	85-89	-0.4214141111426457
1102	90-94	-0.42345764452002044
1102	95-99	-0.7900132425778565
1102	100-104	-0.4710045632252644
1102	105-109	-0.3906770981951837
1102	110-114	-0.3375929032835714
1102	115-119	-0.46451341784980116
1102	120-124	-0.4735507108710877
1102	125-129	-0.5144679622815787
1102	130-134	-0.49021962467065094
1102	135-139	-0.5821952708759284
1102	140-144	-0.5877016797674628
1102	145-149	-0.6696693671674439
1102	150-154	-0.3947693724475627
1102	155-159	-0.478228691277387
1102	160-164	-0.718726085903377
1102	165-169	-0.3936238392277147
1102	170-174	-0.35610846953278497
1102	175-179	-0.10557774980038914
1102	180-184	-0.46537448328724196
1102	185-189	-0.2988812507346026
1102	190-194	-0.664787446925132
1102	195-199	-0.3919722060708182
1102	200-204	-0.48512191826443285
1102	205-209	-0.43608430515715213
1102	210-214	0.18205846635114398
1102	215-219	-0.5160490943463927
1102	220-224	-0.5525961451728776
1102	225-229	-0.7479895116263151
1102	230-234	-0.5116691935696736
1102	235-239	-0.6726774074193287
1102	240-244	-0.6325211697094346
1102	245-249	-0.09845965147864177
1102	250-254	-0.602089210848618
1102	255-259	-0.5895897613989547
1102	260-264	-0.8307550086736057
1102	265-269	-0.8798328527463966
1102	270-274	-0.7437205334798804
1102	275-279	-1.019576642813142
1102	280-284	-0.8389432951335465
1102	285-289	-1.0521369368599096
1102	290-294	-1.0961376482289609
1102	295-299	-0.95255805066558
1102	300-301	-2.7014828841796117
1103	1	-0.37421264819755606
1103	2	0.004867410081793366
1103	3	0.20869917190474752
1103	4	-0.2366064362582705
1103	5	0.12000183852441637
1103	6	-0.27695578517103314
1103	7	-0.15837210056150042
1103	8	-0.15161804777144994
1103	9	-0.2407054278714611
1103	10-14	-0.03314860551021326
1103	15-19	-0.31716846551114486
1103	20-24	-0.161091426129353
1103	25-29	-0.3671314198799607
1103	30-34	-0.1827782242821101
1103	35-39	-0.15092374073856263
1103	40-44	-0.03765634995441047
1103	45-49	-0.11666654648389851
1103	50-54	-0.13765352699104483
1103	55-59	-0.19216810418418362
1103	60-64	-0.07609817822429221
1103	65-69	0.016147440898905074
1103	70-74	-0.21861552919789062
1103	75-79	-0.0895929352435374
1103	80-84	-0.21686140667558362
1103	85-89	-0.2937332314561516
1103	90-94	-0.21481285303613618
1103	95-99	-0.4756656962247021
1103	100-104	-0.38545037243155633
1103	105-109	-0.3421913989497014
1103	110-114	-0.09894518731000801
1103	115-119	-0.34575574106439433
1103	120-124	-0.15018637794717904
1103	125-129	-0.289717421250991
1103	130-134	-0.1098675161135958
1103	135-139	-0.48353468714778813
1103	140-144	-0.5846648896873319
1103	145-149	-0.4024879903177023
1103	150-154	-0.24692121195157313
1103	155-159	-0.25664595746444263
1103	160-164	-0.5034368538743692
1103	165-169	-0.2550404620270328
1103	170-174	-0.5125335733521652
1103	175-179	-0.2702756500168064
1103	180-184	-0.36041571859168187
1103	185-189	-0.2591409454178759
1103	190-194	-0.3758853003497151
1103	195-199	-0.28862308049323104
1103	200-204	-0.2540825546333849
1103	205-209	-0.2157555925175032
1103	210-214	-0.09415607421152572
1103	215-219	-0.748005577939999
1103	220-224	-0.665247487513632
1103	225-229	-0.8583199789597629
1103	230-234	-0.552541861289157
1103	235-239	-0.26554165260854745
1103	240-244	-0.7589620657730691
1103	245-249	0.19284291622329164
1103	250-254	-0.26197340105742484
1103	255-259	-0.4135775955218932
1103	260-264	-0.5503128258345029
1103	265-269	-0.5958578863780275
1103	270-274	-0.39642217704307825
1103	275-279	-0.4514769762049582
1103	280-284	-0.598826315546475
1103	285-289	-0.4493364455456472
1103	290-294	-0.7346052156384459
1103	295-299	-0.7237179032713073
1103	300-301	-1.4224037117561465
1104	1	-0.5065312195405127
1104	2	-0.36502532078654326
1104	3	-0.22912668055524676
1104	4	0.042005656987967654
1104	5	-0.16431529457319982
1104	6	-0.3435705152003976
1104	7	-0.09060677425958374
1104	8	-0.41671063481526005
1104	9	-0.19875660543483065
1104	10-14	-0.401320236120867
1104	15-19	-0.34077965985405
1104	20-24	-0.32275020242227725
1104	25-29	-0.20888607919558666
1104	30-34	-0.2610763006289645
1104	35-39	-0.2357178918741809
1104	40-44	-0.2634560742907155
1104	45-49	-0.422672419319035
1104	50-54	-0.27595040480011335
1104	55-59	-0.3445022565183322
1104	60-64	-0.13000840774428468
1104	65-69	-0.3444494309174786
1104	70-74	-0.28515355280908494
1104	75-79	-0.3837236957157657
1104	80-84	-0.39614348254302456
1104	85-89	-0.5400902758619708
1104	90-94	-0.2804447820826965
1104	95-99	-1.1747008733086446
1104	100-104	-0.6564829127323932
1104	105-109	-0.5031406408745482
1104	110-114	-0.3912062288393443
1104	115-119	-0.3610011416756578
1104	120-124	-0.31202253783212086
1104	125-129	-0.6220575662740586
1104	130-134	-0.5870102020367653
1104	135-139	-0.8703987130362023
1104	140-144	-0.9278733753348405
1104	145-149	-0.6662142442878576
1104	150-154	-0.4161883060967071
1104	155-159	-0.340184041002523
1104	160-164	-0.8750315083958498
1104	165-169	-0.4752693827437682
1104	170-174	-0.7536314339622265
1104	175-179	-0.5416821341062175
1104	180-184	-0.4432155028548763
1104	185-189	-0.42786150633356357
1104	190-194	-0.7143140173150115
1104	195-199	-0.4412065286864362
1104	200-204	-0.4067570916981751
1104	205-209	-0.6266958454089746
1104	210-214	-0.09441735544842089
1104	215-219	-0.7038418576787251
1104	220-224	-0.3634317027710239
1104	225-229	-0.6619084010847658
1104	230-234	-0.5623459003614926
1104	235-239	-0.8101092801029992
1104	240-244	-0.6165182472316886
1104	245-249	0.017209189370063882
1104	250-254	-0.40185234874125086
1104	255-259	-0.5373434012389211
1104	260-264	-0.9834356160304694
1104	265-269	-0.9962605950734229
1104	270-274	-0.6996618246729653
1104	275-279	-1.1115357045563812
1104	280-284	-1.0774551883216859
1104	285-289	-0.6196055172781243
1104	290-294	-1.0579898863402022
1104	295-299	-0.8706479609162514
1104	300-301	-1.8084262184011237
1201	1	-0.0784955283469273
1201	2	-0.18941430307026508
1201	3	-0.016531310065140303
1201	4	-0.42094533227881215
1201	5	0.16285397996449547
1201	6	0.5339673097058437
1201	7	0.18561202827500978
1201	8	-0.21290874680284588
1201	9	-0.1167703129222275
1201	10-14	-0.050718917098492966
1201	15-19	0.026115464935813293
1201	20-24	-0.12220707556336663
1201	25-29	-0.07625967288497293
1201	30-34	-0.10770762094449537
1201	35-39	-0.09471181402599882
1201	40-44	-0.04211894769044022
1201	45-49	-0.01558590696936335
1201	50-54	-0.20855094792697315
1201	55-59	-0.10795757786839744
1201	60-64	-0.26594530157591834
1201	65-69	-0.29349895891437683
1201	70-74	-0.34888043758859766
1201	75-79	-0.3434288554209175
1201	80-84	-0.23811942978213807
1201	85-89	-0.23971525969749052
1201	90-94	-0.32063616964250485
1201	95-99	-0.18470992542297182
1201	100-104	-0.19399746077326085
1201	105-109	-0.27771713124052155
1201	110-114	-0.19304251488615876
1201	115-119	-0.27172143134331606
1201	120-124	-0.19760381288708118
1201	125-129	-0.2867672520363769
1201	130-134	-0.31467087706586483
1201	135-139	-0.13231517495266587
1201	140-144	-0.4284552969693962
1201	145-149	-0.3524938253042791
1201	150-154	-0.24569819876450083
1201	155-159	-0.4212392588998526
1201	160-164	-0.2671432373496856
1201	165-169	-0.3422291654930163
1201	170-174	-0.1799860182115367
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2301	70-74	0.33778810542669646
2301	75-79	0.38277435313522545
2301	80-84	0.29397551345832795
2301	85-89	0.3247842243066401
2301	90-94	0.31082081506525583
2301	95-99	0.9306948967929998
2301	100-104	0.6277681832834077
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2302	1	0.5360430085758807
2302	2	-0.11020752380007082
2302	3	0.09221371789358557
2302	4	-0.023735440078432646
2302	5	0.057821409491211284
2302	6	0.5708843558809775
2302	7	0.13889501668798943
2302	8	-0.20345466252314282
2302	9	0.06020553304537657
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2302	20-24	0.19953155359441865
2302	25-29	0.13501374615002248
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2302	40-44	0.048690990627427766
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2302	50-54	0.21048634958845014
2302	55-59	0.1577114138881086
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2302	70-74	0.16323577847181525
2302	75-79	0.42266095837973694
2302	80-84	0.1873466450434904
2302	85-89	0.2207539857051799
2302	90-94	0.14242487837731943
2302	95-99	0.4580986293703688
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2303	4	0.24881471014462875
2303	5	0.3413939869547207
2303	6	0.4816852420452804
2303	7	0.04527722056647576
2303	8	0.10815955252344622
2303	9	0.16059702344020366
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2303	25-29	0.3090030029252233
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2303	40-44	0.22740969415691126
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2303	50-54	0.21790585127276785
2303	55-59	0.4697966313321942
2303	60-64	0.46962892136407675
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2303	70-74	0.38587424164440165
2303	75-79	0.5167497568987471
2303	80-84	0.5376461807176725
2303	85-89	0.4999904276947902
2303	90-94	0.42191381649756465
2303	95-99	0.8131713130522016
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2303	300-301	1.2633251113212225
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2401	40-44	0.26378830295364963
2401	45-49	0.3436430219964066
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2401	60-64	0.4390050112691313
2401	65-69	0.3448153583473115
2401	70-74	0.38850454584901684
2401	75-79	0.3609417739497047
2401	80-84	0.5570222706227312
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2401	95-99	0.9133117897040108
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2401	245-249	1.9999723721332359
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2402	9	0.007631600953374118
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2402	60-64	0.20610952837365204
2402	65-69	0.030980544512502206
2402	70-74	0.11386364620811662
2402	75-79	0.09391758192551691
2402	80-84	0.40582211942258084
2402	85-89	0.2461505103788042
2402	90-94	0.12250362086570732
2402	95-99	0.7044146058068179
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2403	65-69	0.35333926687122386
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2403	75-79	0.3219274099353413
2403	80-84	0.47854939214985137
2403	85-89	0.5920964563247537
2403	90-94	0.41341271177479655
2403	95-99	0.5491320505242712
2403	100-104	0.6742296179801457
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2404	1	0.39540880104357257
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2404	4	0.12805428540947617
2404	5	0.3033361561290988
2404	6	0.39886066790874963
2404	7	0.15257102824025282
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2404	9	0.19253144687017
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2404	20-24	0.15899361491475617
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2404	30-34	0.26435813666988395
2404	35-39	0.18251746873406205
2404	40-44	0.14999616289033213
2404	45-49	0.07448209817445672
2404	50-54	0.22139448576511
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2404	60-64	0.3043896266537516
2404	65-69	0.1913603387905951
2404	70-74	0.29247642143106134
2404	75-79	0.26418725727993575
2404	80-84	0.366595747992811
2404	85-89	0.2764055508033394
2404	90-94	0.21790432643589952
2404	95-99	0.5638193330081691
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2404	185-189	0.323593360375547
2404	190-194	0.5832926362238524
2404	195-199	0.4719131505349452
2404	200-204	0.5239768559849338
2404	205-209	0.34998940246271104
2404	210-214	0.9617914838112753
2404	215-219	0.5663415548775674
2404	220-224	0.7535442209845655
2404	225-229	0.6972744225048331
2404	230-234	0.5166839108717127
2404	235-239	0.4900007793290868
2404	240-244	0.6328669072382098
2404	245-249	1.4739153782796421
2404	250-254	0.5732099686939378
2404	255-259	0.5219494817827695
2404	260-264	0.6765176235837842
2404	265-269	0.7575656062104059
2404	270-274	0.5823151996769482
2404	275-279	0.7820433686837163
2404	280-284	0.7910089174305526
2404	285-289	0.6404928818033255
2404	290-294	0.7559942056100013
2404	295-299	0.903996028105297
2404	300-301	2.965572514497289
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	33.0
16	206.0
17	197.0
18	161.0
19	158.0
20	146.0
21	157.0
22	148.0
23	197.0
24	222.0
25	221.0
26	270.0
27	285.0
28	377.0
29	412.0
30	522.0
31	529.0
32	736.0
33	946.0
34	1340.0
35	1821.0
36	2864.0
37	5150.0
38	15991.0
39	94193.0
40	503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.69245216574716	0.27311499784794774	0.021129240521187934	0.01330359588371092
2	0.5117971592909966	99.24326016355597	0.015651289274954024	0.22929138787807646
3	0.28485346480416324	0.1377313456195954	0.22616113002308563	99.35125405955316
4	0.2770278201666862	0.1267754431271276	99.31447352975702	0.2817232069491724
5	0.3286770747740345	98.47008647337324	0.07199593066478852	1.1292405211879328
6	0.22381343663184253	0.49614587001604255	0.1291231365183707	99.15091755683375
7	0.38110889384513047	31.373791916109088	37.329107485229095	30.915991704816687
8	34.27710607661306	33.399068748288144	0.1275580075908753	32.196267167507926
9	99.65801932934225	0.22068317877685173	0.04304104550612357	0.07825644637477011
10-14	40.07152639198654	19.766326251124937	39.771178150800175	0.39096920608835156
15-19	0.2580897601439919	39.61623038697813	39.70466017138162	20.421019681496265
20-24	20.032241655906404	0.128340572054623	39.89091051375357	39.9485072582854
25-29	20.080604139766013	0.04757991939586023	59.067809210783736	20.80400673005439
30-34	0.23696051962280393	20.14915678679031	0.23586492937355713	79.37801776421333
35-39	20.02253785655593	0.2061274797511445	40.367335759283165	39.40399890440975
40-44	21.80772391125719	18.57322846969519	21.20859255781195	38.41045506123567
45-49	18.424697734475878	19.930195249833705	19.99671322925226	41.648393786438156
50-54	39.734740703873086	0.4548286019263697	21.217926444855554	38.59250424934499
55-59	20.605240092656356	21.058191948913795	39.90421336004508	18.432354598384773
60-64	20.149314468165027	2.34692919301321	37.29042759656921	40.21332874225255
65-69	57.952795342139865	21.853909722656983	0.22444124460026293	19.96885369060289
70-74	57.82226256808364	2.030927189632505	0.2886120328053591	39.858198209478495
75-79	0.5058536279972454	0.06041444938333437	39.86539785888687	59.56833406373255
80-84	19.909378325924997	21.048644587741812	0.18875602579352657	58.85322106053966
85-89	41.86564828147499	17.977368058598884	22.13876541664058	18.018218243285546
90-94	0.1615225693357541	0.1316283728792337	59.9588367870782	39.748012270706816
95-99	18.317786264321043	39.84239028360358	2.3034182683278033	39.536405183747576
100-104	0.14821886934201467	2.2586552307018093	59.32229387090716	38.27083202904902
105-109	20.08044825643273	39.49352031553246	0.46688161272146744	39.959149815313346
110-114	0.17482626932949352	19.887779377699868	58.93961685344018	20.997777499530457
115-119	20.102829775245727	0.4758029174231516	19.738308395417267	59.683058911913854
120-124	0.21442434107556502	40.02629437175233	1.752801602704564	58.006479684467536
125-129	40.43557878920679	40.757997871408	18.49057785012208	0.31584548926313155
130-134	0.19345144932072872	19.77117636010768	57.499373943529704	22.53599824704188
135-139	0.17028735991986477	0.6025793526576096	3.899236211106242	95.32789707631628
140-144	0.14712327051900081	17.802698303386965	40.11456833406373	41.9356100920303
145-149	38.04451261503788	38.01555750328679	2.0201277155199397	21.919802166155385
150-154	0.5086716782565552	39.558692103380864	20.034934005719034	39.897702212643544
155-159	37.64665372816628	1.0470794465660802	40.913572904275966	20.39269392099167
160-164	20.24823139047142	20.871314092531147	20.084674137607212	38.79578037939022
165-169	18.517028735991985	38.62956238652727	22.584674137607212	20.268734739873537
170-174	21.923715019094722	37.77389970575346	19.986070243535966	20.316315031615854
175-179	17.950604144493834	3.1820885243848998	40.220684905778505	38.64662242534276
180-184	40.07716145996369	18.304013021974583	21.39782758404808	20.22099793401365
185-189	39.85099856007012	20.084987165842357	18.61312840418206	21.450885869905463
190-194	57.953108370375006	2.6992424716709444	1.6469980592249422	37.700651098729104
195-199	18.61234583359419	18.23592938083015	59.65410380016277	3.4976209854128846
200-204	0.3762599386464659	38.561948287735554	19.79966192950604	41.26212984411194
205-209	19.921116884743004	20.740624804357353	21.04019282539285	38.2980654855068
210-214	1.6393184392214064	40.97603006756065	38.60077468704565	18.78387680617229
215-219	1.0912164277217804	58.451762348963875	0.15119263757590937	40.30582858573843
220-224	36.71054279095974	1.8158767920866463	19.99264383647405	41.48093658047956
225-229	38.00272334564578	1.9584611531960183	18.951355412258188	41.087460088900016
230-234	18.269736430226004	1.629624992174294	59.7074751142553	20.393163463344393
235-239	19.936298754147625	18.653352532398422	0.25871783634883866	61.15163087710511
240-244	19.520127715519937	41.57437550867088	20.13507168346585	18.77042509234333
245-249	1.841749622252254	19.525290351197704	40.067428172083844	38.5655318544662
250-254	20.272647592812874	41.27136417704877	37.27446315657673	1.1815250735616352
255-259	20.047580291742314	19.72062230013147	20.235553746947975	39.99624366117824
260-264	19.95961935766606	18.6675953170976	18.646465911225192	42.726319414011144
265-269	1.6584235898078008	2.247386214236524	20.071526951731048	76.02266324422463
270-274	18.418412464881868	21.39815155380606	59.53139306480518	0.6520429165068906
275-279	21.63507364921655	18.51083177989911	0.311625948690498	59.542468622193844
280-284	58.07044865825123	0.24933284291091792	20.041946768298885	21.638271730538968
285-289	40.00187821350592	1.7146524131130607	19.985130809744795	38.29833856363622
290-294	39.66755620945211	38.20129753249701	2.0312879066527887	20.099858351398094
295-299	38.31738830259395	20.003225013581062	39.77271481956362	1.9066718642613703
300-301	8.171258587718677	3.0299596288689	0.3173029251363411	88.48147885827609
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.8333333333333333
46	2.25
47	116.0
48	130.75
49	13.166666666666666
50	27.083333333333325
51	341.25
52	2262.083333333334
53	754.5
54	201.66666666666666
55	2264.9166666666665
56	25923.249999999956
57	8674.416666666655
58	230.58333333333331
59	54.583333333333336
60	25.333333333333332
61	15.916666666666668
62	8.25
63	7.666666666666666
64	7.333333333333333
65	6.25
66	5.0
67	5.833333333333334
68	4.083333333333333
69	4.25
70	3.75
71	3.3333333333333335
72	1.5
73	3.5
74	3.75
75	2.75
76	2.083333333333333
77	2.9166666666666665
78	2.9166666666666665
79	2.583333333333333
80	2.3333333333333335
81	1.4166666666666665
82	0.9166666666666666
83	2.0
84	1.3333333333333333
85	0.8333333333333333
86	1.3333333333333333
87	1.0
88	1.5
89	1.8333333333333333
90	0.5
91	0.25
92	1.25
93	0.75
94	1.0833333333333333
95	1.0
96	1.0
97	0.8333333333333333
98	1.3333333333333333
99	9.25
100	11.333333333333336
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	1.5651411757340513E-4
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.6390471420522131
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	1.906185437926501
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	1.0
270-274	0.0
275-279	2.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	300.0
300-302	127481.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	3.777438666510154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.04909881914233	2.608287357671088
2	9.0532421794075	0.6839613413154908
3	3.7083074373316762	0.4202371170325156
4	2.5896001657344105	0.3912822318738506
5	1.885228920654651	0.35606683100520403
6	1.2222912782266417	0.2770278201666862
7	1.3880256888336442	0.36702273349767184
8	1.1187072715972655	0.33806784833900694
9	0.7665216490573856	0.2605939664279845
>10	8.038118914439611	5.823844739210393
>50	0.6007872384503833	1.4328755331220409
>100	0.20716801325875284	1.9157178072543726
>500	0.18645121193287756	5.231443440153383
>1k	0.0	0.0
>5k	0.020716801325875285	7.579136831396487
>10k+	0.16573441060700228	72.31443440153382
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	13036	10.201510349415033	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	12763	9.98787025081191	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	12699	9.937786125132057	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	11263	8.814023555190358	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	10989	8.599600892123489	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	10823	8.469695191141371	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	10528	8.238838674335797	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	10306	8.065109363383808	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	9685	7.579136831396487	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	879	0.6878741636342294	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	839	0.6565715850843213	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	824	0.6448331181281057	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	737	0.5767500097820558	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	708	0.5540556403333724	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	687	0.5376217865946707	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	682	0.5337089642759322	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	674	0.5274484485659506	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	655	0.5125797237547444	No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	326	0.2551160151817506	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	277	0.21677035645811324	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	265	0.20737958289314082	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	262	0.2050318895018977	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	247	0.1932934225456822	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	232	0.18155495558946666	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	232	0.18155495558946666	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	226	0.17685956880698048	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	212	0.16590366631451264	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	169	0.1322533943733615	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
