##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAFS2_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127785
Sequences flagged as poor quality	0
Sequence length	51-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.721281840591615	40.0	40.0	40.0	40.0	40.0
2	39.303658488868024	40.0	40.0	40.0	40.0	40.0
3	39.22022146574324	40.0	40.0	40.0	40.0	40.0
4	38.9781586258168	40.0	40.0	40.0	40.0	40.0
5	39.30473842782799	40.0	40.0	40.0	40.0	40.0
6	39.30805650115428	40.0	40.0	40.0	40.0	40.0
7	39.36640450757131	40.0	40.0	40.0	40.0	40.0
8	39.40985248659858	40.0	40.0	40.0	40.0	40.0
9	38.99938177407364	40.0	40.0	40.0	40.0	40.0
10-14	39.5967100989944	40.0	40.0	40.0	40.0	40.0
15-19	39.49561529130962	40.0	40.0	40.0	40.0	40.0
20-24	39.45493446022617	40.0	40.0	40.0	40.0	40.0
25-29	39.617388582384464	40.0	40.0	40.0	40.0	40.0
30-34	39.491583519192396	40.0	40.0	40.0	40.0	40.0
35-39	39.62607817818993	40.0	40.0	40.0	40.0	40.0
40-44	39.57792855186447	40.0	40.0	40.0	40.0	40.0
45-49	39.433967993113434	40.0	40.0	40.0	40.0	40.0
50-54	39.552872072357715	40.0	40.0	40.0	40.0	40.0
55-59	39.41682213735679	40.0	40.0	40.0	40.0	40.0
60-64	39.346722594378015	40.0	40.0	40.0	40.0	40.0
65-69	39.48028548175046	40.0	40.0	40.0	40.0	40.0
70-74	39.38885619482877	40.0	40.0	40.0	40.0	40.0
75-79	39.41391097476993	40.0	40.0	40.0	40.0	40.0
80-84	39.47326738871847	40.0	40.0	40.0	40.0	40.0
85-89	39.58063607337382	40.0	40.0	40.0	40.0	40.0
90-94	39.44543917861391	40.0	40.0	40.0	40.0	40.0
95-99	39.22439116008265	40.0	40.0	40.0	36.8	40.0
100-104	39.35638890627935	40.0	40.0	40.0	40.0	40.0
105-109	39.3635322106054	40.0	40.0	40.0	40.0	40.0
110-114	39.35419770863332	40.0	40.0	40.0	40.0	40.0
115-119	39.32848556939835	40.0	40.0	40.0	40.0	40.0
120-124	39.174970262317665	40.0	40.0	40.0	40.0	40.0
125-129	39.36445877418143	40.0	40.0	40.0	40.0	40.0
130-134	39.343097727415014	40.0	40.0	40.0	40.0	40.0
135-139	39.050848306517246	40.0	40.0	40.0	40.0	40.0
140-144	39.40772553684342	40.0	40.0	40.0	40.0	40.0
145-149	38.96902898641458	40.0	40.0	40.0	36.8	40.0
150-154	39.23419520440744	40.0	40.0	40.0	40.0	40.0
155-159	39.233562887372436	40.0	40.0	40.0	40.0	40.0
160-164	39.37922118575095	40.0	40.0	40.0	40.0	40.0
165-169	39.276078382270086	40.0	40.0	40.0	40.0	40.0
170-174	38.99635009077819	40.0	40.0	40.0	40.0	40.0
175-179	39.2901959556752	40.0	40.0	40.0	40.0	40.0
180-184	39.34808113691855	40.0	40.0	40.0	40.0	40.0
185-189	39.25225067301071	40.0	40.0	40.0	40.0	40.0
190-194	39.3366806485945	40.0	40.0	40.0	40.0	40.0
195-199	39.25730920929068	40.0	40.0	40.0	40.0	40.0
200-204	39.48466161647781	40.0	40.0	40.0	40.0	40.0
205-209	39.28546296875978	40.0	40.0	40.0	40.0	40.0
210-214	39.354535779127275	40.0	40.0	40.0	40.0	40.0
215-219	39.34694797470731	40.0	40.0	40.0	40.0	40.0
220-224	39.26260564702936	40.0	40.0	40.0	40.0	40.0
225-229	39.37410630438866	40.0	40.0	40.0	40.0	40.0
230-234	39.19045889939273	40.0	40.0	40.0	40.0	40.0
235-239	39.35234458148125	40.0	40.0	40.0	40.0	40.0
240-244	39.25115507418769	40.0	40.0	40.0	40.0	40.0
245-249	39.243717523320605	40.0	40.0	40.0	40.0	40.0
250-254	39.37962186189195	40.0	40.0	40.0	40.0	40.0
255-259	39.19328867463845	40.0	40.0	40.0	40.0	40.0
260-264	39.09584924560196	40.0	40.0	40.0	40.0	40.0
265-269	39.15836724472548	40.0	40.0	40.0	40.0	40.0
270-274	37.325403222650905	40.0	36.8	40.0	31.2	40.0
275-279	39.125682690832	40.0	40.0	40.0	40.0	40.0
280-284	38.191499518707786	40.0	40.0	40.0	36.8	40.0
285-289	39.08199810613471	40.0	40.0	40.0	40.0	40.0
290-294	38.98045340243062	40.0	40.0	40.0	40.0	40.0
295-299	38.910479631707595	40.0	40.0	40.0	40.0	40.0
300-301	38.274739092409305	40.0	40.0	40.0	32.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.03484352790405154
1101	2	-0.08277499342160866
1101	3	-0.10148497988815564
1101	4	-0.022838002901877985
1101	5	-0.1090680931524588
1101	6	-0.008785375315184751
1101	7	-0.299162851792822
1101	8	-0.12217197927613199
1101	9	0.18192474454336605
1101	10-14	-0.14180378907926183
1101	15-19	4.215544165617757E-4
1101	20-24	-0.20491940860464553
1101	25-29	-0.05776799829984469
1101	30-34	-0.13481112794036676
1101	35-39	-0.11134977373226462
1101	40-44	-0.09733974189425965
1101	45-49	-0.16515994197032313
1101	50-54	0.07314136767757162
1101	55-59	-0.13408707160196087
1101	60-64	-0.07651633222029375
1101	65-69	-0.04069435719006265
1101	70-74	-0.08289269850429548
1101	75-79	0.01643776801745389
1101	80-84	-0.023849303431923374
1101	85-89	0.013679416857883098
1101	90-94	-0.01714310017811016
1101	95-99	0.053146350857723235
1101	100-104	-0.03253324788016698
1101	105-109	-0.11727041786031833
1101	110-114	0.00909623442831986
1101	115-119	-0.07063190749921944
1101	120-124	-0.03941553732609293
1101	125-129	0.07985487692698001
1101	130-134	0.0019891222606389647
1101	135-139	-0.07790618884726541
1101	140-144	-0.030768106560678632
1101	145-149	-0.04334292905625858
1101	150-154	-0.0032594078426058104
1101	155-159	-0.050733588996493495
1101	160-164	-0.0748506101254236
1101	165-169	-0.008261318682130536
1101	170-174	0.04140305384023435
1101	175-179	0.04316003694717807
1101	180-184	-0.12125123611571098
1101	185-189	-0.01893015906348694
1101	190-194	-0.14510465438690545
1101	195-199	0.0057301409908134815
1101	200-204	-0.045808731187932494
1101	205-209	-0.19794794054791964
1101	210-214	0.024946254515612054
1101	215-219	-0.06794154040578348
1101	220-224	-0.11003534656610015
1101	225-229	-0.0513862818869697
1101	230-234	-0.24590933077987387
1101	235-239	-0.16164918143197582
1101	240-244	-0.1454646742365142
1101	245-249	-0.244804750641471
1101	250-254	-0.0313310540215852
1101	255-259	-0.06363640139468885
1101	260-264	-0.196348340598675
1101	265-269	-0.214394344009321
1101	270-274	0.26163648711777654
1101	275-279	-0.13048034828403132
1101	280-284	-0.10582067991409616
1101	285-289	-0.06348407894654429
1101	290-294	-0.2096121255579888
1101	295-299	-0.32649241419481
1101	300-301	-0.10821773504910226
1102	1	0.0848574702385605
1102	2	-0.27107458630544556
1102	3	0.34913127129694743
1102	4	-0.2012329613537176
1102	5	0.20259327542709826
1102	6	0.19881040852286702
1102	7	-0.1273040597566819
1102	8	0.17204474037141182
1102	9	0.3569735427141296
1102	10-14	0.08106833182866069
1102	15-19	0.017201432839407005
1102	20-24	-0.01592381438234014
1102	25-29	-0.06430416471555844
1102	30-34	0.021097633422641593
1102	35-39	0.038036205856158745
1102	40-44	0.04094590865506831
1102	45-49	-0.012325186518872044
1102	50-54	0.013231714005158324
1102	55-59	-0.03561180530337538
1102	60-64	0.004286746247153417
1102	65-69	0.11945287386231485
1102	70-74	0.04848647722489119
1102	75-79	-0.045550966576264784
1102	80-84	0.04630406224762851
1102	85-89	-0.0394263775763406
1102	90-94	-0.08011783666798067
1102	95-99	-0.03510751116893829
1102	100-104	-0.023877846619782872
1102	105-109	-0.057101994459500816
1102	110-114	-0.12288078860441232
1102	115-119	-0.08923655866201585
1102	120-124	-0.20288662092433185
1102	125-129	-0.053175071207540725
1102	130-134	-0.009008591196497662
1102	135-139	-0.052774294362848195
1102	140-144	-0.023745631517464005
1102	145-149	-0.05198494567552103
1102	150-154	-0.11203192471282364
1102	155-159	-0.090146895560558
1102	160-164	-0.03753423067085038
1102	165-169	-0.08314392422558825
1102	170-174	-0.013697052121507625
1102	175-179	-0.09090945164798825
1102	180-184	0.015365795840125429
1102	185-189	-0.027710483641037342
1102	190-194	-0.10173395559710485
1102	195-199	0.005435678886072992
1102	200-204	-0.01733959951653219
1102	205-209	-0.03609193697867852
1102	210-214	0.016853008182444285
1102	215-219	0.009256608052197635
1102	220-224	0.01862228546780642
1102	225-229	0.13644038701047378
1102	230-234	0.08333053314294148
1102	235-239	-0.034040012952807785
1102	240-244	-0.28434371054235186
1102	245-249	-0.0322477264933454
1102	250-254	0.04173570187602138
1102	255-259	0.01578359748992142
1102	260-264	-0.16900351878170738
1102	265-269	0.07164882145830376
1102	270-274	0.13850671186821017
1102	275-279	-0.11738570771741053
1102	280-284	-0.22363675263731864
1102	285-289	-0.10428046509344568
1102	290-294	-0.2233001518811193
1102	295-299	-0.027979224746374598
1102	300-301	0.05116113828770352
1103	1	0.08158204180082151
1103	2	-0.044204375157292475
1103	3	0.04825977337333143
1103	4	0.16706557055246662
1103	5	0.058146881322869604
1103	6	0.10216187369218943
1103	7	-0.16008174005147424
1103	8	-0.10833193389893125
1103	9	0.36502173830400153
1103	10-14	-0.08635285317455299
1103	15-19	0.21759682384367807
1103	20-24	0.0795502453942234
1103	25-29	0.05463132104218715
1103	30-34	0.030044232241138502
1103	35-39	0.02618851327851246
1103	40-44	0.14125356496904828
1103	45-49	0.051470801081286766
1103	50-54	0.13639718163105385
1103	55-59	0.09234514620858647
1103	60-64	-0.03413402932751097
1103	65-69	-0.01855028458654573
1103	70-74	0.05423719372486602
1103	75-79	0.007997609197268218
1103	80-84	0.07661581284714458
1103	85-89	0.0287446465799448
1103	90-94	0.2167763552955222
1103	95-99	0.10101929470344118
1103	100-104	-0.022141869547787962
1103	105-109	0.06918448854921166
1103	110-114	0.12303837850092947
1103	115-119	0.07466588036237454
1103	120-124	0.10719818927628921
1103	125-129	0.03229957346696466
1103	130-134	0.04391765317386387
1103	135-139	0.1386791770064022
1103	140-144	0.061120719304327054
1103	145-149	0.023134665952248668
1103	150-154	0.05618472148240983
1103	155-159	-0.021147655927862274
1103	160-164	0.05633486917123065
1103	165-169	0.146837943938408
1103	170-174	-0.029203866182456295
1103	175-179	-0.06560910655820607
1103	180-184	-0.042567174856486645
1103	185-189	-0.03912301215481051
1103	190-194	-0.03701616885088299
1103	195-199	-0.08148483348451663
1103	200-204	0.07108293075194183
1103	205-209	-0.06236087304933591
1103	210-214	0.039149317476478984
1103	215-219	-0.08568402480736381
1103	220-224	-0.10504385479072909
1103	225-229	-0.039815096407672
1103	230-234	0.03361420864155917
1103	235-239	-0.09675978835200283
1103	240-244	-0.03451742522914003
1103	245-249	-0.13735154587686083
1103	250-254	-0.007508025093621029
1103	255-259	-0.07039760389044147
1103	260-264	-0.0974260490501706
1103	265-269	-0.09732117327761358
1103	270-274	-0.015981209989433864
1103	275-279	0.01608800112038722
1103	280-284	-0.01474864361234296
1103	285-289	0.041836397514025236
1103	290-294	-0.08078626055515059
1103	295-299	0.028629537024698948
1103	300-301	-0.06343818036842919
1104	1	-0.11641640010005716
1104	2	-0.1285095228770743
1104	3	0.14078689029069125
1104	4	-0.19350253635710857
1104	5	0.11265637973480125
1104	6	0.10745941069704656
1104	7	0.22706993490508154
1104	8	0.07322256960679141
1104	9	-0.052644708630737114
1104	10-14	-0.04867082524862809
1104	15-19	0.014962437794658001
1104	20-24	-0.07733355636762695
1104	25-29	-0.03242087527831217
1104	30-34	-0.005058542373831187
1104	35-39	-0.03938588595442383
1104	40-44	0.05837707428767658
1104	45-49	0.09268132424303133
1104	50-54	0.014136739370613327
1104	55-59	-0.0980114187821215
1104	60-64	-0.10014469631377665
1104	65-69	-0.04432843524273267
1104	70-74	-0.00697932212578678
1104	75-79	-0.163364528994137
1104	80-84	-0.11758566135431892
1104	85-89	-0.02134943863609351
1104	90-94	-0.08669341158888244
1104	95-99	-0.1478067375372305
1104	100-104	0.004173226035604216
1104	105-109	-0.021659881319543217
1104	110-114	-0.10902730136962901
1104	115-119	-0.10299577242123092
1104	120-124	-0.23035319803120302
1104	125-129	-0.13543339231478058
1104	130-134	-0.054990478905004636
1104	135-139	-0.2782010291421031
1104	140-144	-0.141417783721991
1104	145-149	-0.0668707274677729
1104	150-154	-0.041520078661413606
1104	155-159	-0.13714693680519474
1104	160-164	-0.08749445904834374
1104	165-169	-0.09531462992148221
1104	170-174	-0.08402977856934513
1104	175-179	-0.2344926686125035
1104	180-184	-0.09299685065202112
1104	185-189	-0.16094718666191454
1104	190-194	-0.23492352188019083
1104	195-199	-0.21933585523798627
1104	200-204	-0.20936052822810325
1104	205-209	-0.36087348765950367
1104	210-214	-0.23859982856535566
1104	215-219	-0.18942945538207567
1104	220-224	-0.2017466773472023
1104	225-229	-0.1273179429349014
1104	230-234	-0.28081019358771186
1104	235-239	-0.1731593808491496
1104	240-244	-0.3291707481751516
1104	245-249	-0.4505438512642854
1104	250-254	-0.1832903706320579
1104	255-259	-0.3090120942122496
1104	260-264	-0.3307909438784833
1104	265-269	-0.25547542509040255
1104	270-274	-0.2775692725531016
1104	275-279	-0.09041406635728322
1104	280-284	-0.248473099775822
1104	285-289	-0.2635366473712111
1104	290-294	-0.23835562592940107
1104	295-299	-0.2886492508344105
1104	300-301	-0.2995101807322058
1201	1	0.01833078727870685
1201	2	-0.029453529084236152
1201	3	-0.0994820868203874
1201	4	-0.11203350751965502
1201	5	-0.16951095506411207
1201	6	-0.06789297518165682
1201	7	-0.1637227716771008
1201	8	-0.5350795123794967
1201	9	0.014082305464697242
1201	10-14	-0.01577285550855123
1201	15-19	-0.07731740185360536
1201	20-24	-0.02410712419382577
1201	25-29	-0.05937031275406213
1201	30-34	-0.06764744706799775
1201	35-39	0.04078998369512732
1201	40-44	0.015133831044430224
1201	45-49	-0.16015716017439985
1201	50-54	-0.04121047387133814
1201	55-59	-0.04369873289049053
1201	60-64	-0.06879851759917699
1201	65-69	-0.049863156566928524
1201	70-74	-0.05156745092445192
1201	75-79	-0.13589782784323035
1201	80-84	-0.03859949815764452
1201	85-89	-0.17802860584156832
1201	90-94	-0.10531490389458043
1201	95-99	-0.18815330419958798
1201	100-104	-0.2978053075218767
1201	105-109	-0.18935676480590047
1201	110-114	-0.18796173819880124
1201	115-119	-0.20598297014527134
1201	120-124	-0.2654754015744558
1201	125-129	-0.30794082798011146
1201	130-134	-0.17199552222584202
1201	135-139	-0.17092054291424574
1201	140-144	-0.2710440607166831
1201	145-149	-0.369004440127803
1201	150-154	-0.30894539871831483
1201	155-159	-0.21246052790826297
1201	160-164	-0.30386585436710334
1201	165-169	-0.22090625024993926
1201	170-174	-0.11460006177120619
1201	175-179	-0.13683328674260054
1201	180-184	-0.2146572670492759
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2404	215-219	0.014617162223871105
2404	220-224	0.15490299455670709
2404	225-229	0.1452058685719564
2404	230-234	0.08584358323555108
2404	235-239	0.1354306090967654
2404	240-244	0.08345812547067766
2404	245-249	0.10155709405699298
2404	250-254	0.10760920162343979
2404	255-259	-0.01926903836749716
2404	260-264	0.21390448895253655
2404	265-269	0.21931025647494096
2404	270-274	0.21856198967986273
2404	275-279	0.15203555914318656
2404	280-284	0.06255366548823105
2404	285-289	0.15487956765517197
2404	290-294	0.26432446997103654
2404	295-299	0.11205480130155365
2404	300-301	0.25077835534500537
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	8.0
18	15.0
19	32.0
20	34.0
21	41.0
22	78.0
23	155.0
24	233.0
25	264.0
26	253.0
27	263.0
28	289.0
29	319.0
30	419.0
31	443.0
32	588.0
33	676.0
34	816.0
35	1115.0
36	1565.0
37	2453.0
38	4902.0
39	102888.0
40	9935.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.057127205853582184	0.009390773564972414	0.10955902492467817	99.82392299565677
2	0.008608276466537283	0.011738558818005385	0.1502535528704689	99.82939961184499
3	0.005477951246233908	0.13147082990961378	99.60167468795242	0.2613765308917322
4	0.07982157530226552	99.38412176703056	0.10173338028720115	0.4343232773799742
5	0.1956411159369253	0.13147082990961378	0.09782055796846265	99.575067496185
6	99.76601322533944	0.08060413976601322	0.016433853738701723	0.13694878115584772
7	99.89904918417655	0.061040028172320696	0.032867707477403446	0.007043080173729311
8	99.74018859803576	0.11347184724341668	0.08608209101224713	0.06025746370857299
9	29.938568689595808	21.270102124662518	26.21669210001174	22.574637085729936
10-14	59.82564463747701	10.303556755487733	9.704425402042494	20.16637320499276
15-19	39.668192667370974	20.374848378135148	19.939899049184177	20.0170599053097
20-24	59.917987244199246	39.839104746253476	0.21207496967562703	0.030833039871659428
25-29	19.388191102242047	19.565989748405524	59.70340806823962	1.3424110811128067
30-34	0.5056931564737646	59.55112102359432	0.23210861994756815	39.71107719998435
35-39	59.74050162382126	0.6420158860586141	19.834409359470985	19.783073130649136
40-44	39.64972414602653	41.74871855069061	0.07246546934303713	18.52909183393982
45-49	20.104707125249444	19.92878663379896	20.634659780099383	39.33184646085221
50-54	40.39100234457414	20.055186705106415	19.66531125865129	19.888499691668155
55-59	20.091560758780442	21.832780316784575	18.142490452638828	39.93316847179615
60-64	39.97041883177862	0.06870969761472485	0.325705878670256	59.63516559193639
65-69	22.181180742502974	20.113003192887998	56.33976084642834	1.3660552181806798
70-74	40.023320603518435	58.50529017717398	1.3574469417141426	0.11394227759343892
75-79	40.377042509234336	19.873693107118264	1.0804169536092156	38.66884743003819
80-84	40.05509296938584	0.14994052463532212	39.69604958367245	20.098916922306394
85-89	39.14887622863582	0.5715895573780755	57.99661929506041	2.282914918925687
90-94	20.0247292305766	59.69010204720466	18.22810367495148	2.0570650472672636
95-99	1.891473110874601	17.853721905715897	59.81014837538346	20.444656608026044
100-104	37.390909660051335	20.080135228197584	2.577944030551556	39.951011081199525
105-109	76.43163463344393	22.993175984473798	0.44731734802479184	0.12787203405747197
110-114	56.908063607337375	41.611782382770926	1.2651036123458337	0.21505039754585864
115-119	39.387247229700115	39.22431603330621	19.844424967132035	1.5440117698616413
120-124	21.198898140612283	38.02635697739936	39.09753959807175	1.6772052839166094
125-129	4.025856132223127	56.16477806298128	20.572841670318663	19.23652413447693
130-134	15.940806360733738	10.164496337569648	54.667564014274085	19.227133287422525
135-139	1.6178864333562886	19.02695173104614	37.999593063294306	41.355568772303265
140-144	39.84411193889689	19.810461403618607	20.053527828210104	20.291898829274402
145-149	0.6593939773367558	21.909002692042822	38.298535027859515	39.13306830276091
150-154	38.90158392286984	18.834595880548424	2.873286170412571	39.39053402616916
155-159	21.724316033306206	76.36824641582672	0.058223251737306705	1.8492142991297815
160-164	21.56827145808552	18.634101295936894	0.47971577036248675	59.317911475615105
165-169	38.443154072497336	21.495022851061165	21.219558004131972	18.842265072309523
170-174	1.7445063544731736	21.66812746509735	20.071526951731048	56.515839228698425
175-179	43.56946096537907	0.6041444938333438	18.726131597070054	37.100262943717524
180-184	38.71267138295874	38.38493082076003	1.3101796782069743	21.59221811807425
185-189	38.87575909347023	1.4364865710887122	4.216177299192387	55.47157703624867
190-194	21.27308583234208	38.529080323045136	37.303731296562944	2.894102548049834
195-199	37.067708007262254	39.777123896575475	3.3540975395980714	19.801070556564202
200-204	77.97189006448382	0.27984724222124835	20.08780441995868	1.6604582733362552
205-209	20.508670882113567	40.95004069367057	1.5779753333750706	36.96331309084079
210-214	24.231202654479432	36.464189569899204	38.91347899580542	0.3911287798159394
215-219	39.91814311650911	20.1594878858073	19.448757277906466	20.473611719777125
220-224	4.5670819507919616	36.48265823577287	40.59444061854379	18.355819194891378
225-229	56.450259813435174	0.3340011269016465	23.24375508670882	19.97198397295436
230-234	20.138828022287612	58.191948913792025	20.09641269642522	1.5728103674951481
235-239	41.20688036060853	21.13394478181932	0.6248043573530333	37.03437050021912
240-244	38.77386840292994	22.64790584110687	2.0450134602141112	36.53321229574908
245-249	57.7454141363551	1.8773868402929945	20.291116258686532	20.086082764665374
250-254	21.08417329243098	1.3831152569961809	74.19160458273336	3.3411068678394793
255-259	18.678238277092593	39.790897138921935	18.93648657108871	22.59437801289676
260-264	39.813435171852504	37.59109121642773	21.93138421085582	0.664089400863958
265-269	20.42055343391974	58.17692355850498	18.655700244162023	2.74682276341326
270-274	2.2898194595525228	2.1223480431669315	22.507845331538622	73.07998716574193
275-279	55.99782127915331	3.513030847995092	19.695354722987855	20.793793149863752
280-284	2.758313051236099	40.77147619755676	1.1236412299168108	55.346569521290334
285-289	56.87966129549776	20.883542936743336	20.5277779951636	1.7090177725952997
290-294	21.670444086876493	20.05058821319676	20.462087014231063	37.81688068569568
295-299	22.671067092931867	21.696783910471783	36.71010730735871	18.92204168923764
300-301	6.863973398261747	48.2507150793088	44.87309804663184	0.012213475797618766
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.25
44	0.25
45	0.25
46	0.5833333333333333
47	0.5833333333333333
48	2.0
49	13.833333333333334
50	685.0833333333334
51	514.1666666666665
52	34.916666666666664
53	221.83333333333334
54	16194.333333333348
55	25421.16666666658
56	1204.3333333333342
57	107.24999999999997
58	29.91666666666666
59	9.333333333333334
60	3.833333333333333
61	0.75
62	0.0
63	0.5833333333333333
64	1.0
65	0.0
66	0.0
67	0.25
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.825644637477012E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	1.0
270-274	0.0
275-279	2.0
280-284	0.0
285-289	1.0
290-294	4.0
295-299	456.0
300-302	127320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.3789959697930114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.19078947368421	1.3843565363696835
2	12.631578947368421	0.6010095081582345
3	5.0	0.3568493954689517
4	3.421052631578948	0.3255468169190437
5	1.9407894736842106	0.23085651680557184
6	1.7434210526315788	0.24885549947176897
7	1.348684210526316	0.22459600109559025
8	1.4802631578947367	0.2817232069491724
9	1.118421052631579	0.23946472590679657
>10	11.677631578947368	5.754979066400595
>50	0.4276315789473685	0.6839613413154908
>100	0.4934210526315789	2.5002934616739054
>500	0.2302631578947368	4.532613374026686
>1k	0.03289473684210526	0.8138670422976092
>5k	0.03289473684210526	7.006299643933168
>10k+	0.2302631578947368	74.81472786320774
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	16475	12.89274954024338	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	14591	11.41839809054271	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	14455	11.311969323473022	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	13283	10.394803771960715	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	13006	10.178033415502602	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	12614	9.871268145713504	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	11178	8.747505575771804	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8953	7.006299643933168	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	1040	0.8138670422976092	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	976	0.7637829166177563	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	945	0.7395234182415776	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	894	0.6996126305904449	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	847	0.662832100794303	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	808	0.6323120867081425	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	750	0.5869233478107759	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	572	0.44762687326368505	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	339	0.2652893532104707	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	335	0.2621590953554799	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	321	0.25120319286301207	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	313	0.24494267715303047	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	311	0.24337754822553506	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	264	0.2065970184293931	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	248	0.1940759870094299	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	196	0.15338263489454942	No Hit
CCTACGGGAGGCTGCCGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	180	0.1408616034745862	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	140	0.10955902492467817	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
