##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	IAFS1_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	164482
Sequences flagged as poor quality	0
Sequence length	51-301
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.788353740834864	40.0	40.0	40.0	40.0	40.0
2	39.319913425177226	40.0	40.0	40.0	40.0	40.0
3	39.36574214807699	40.0	40.0	40.0	40.0	40.0
4	39.243734876764634	40.0	40.0	40.0	40.0	40.0
5	39.31387021072214	40.0	40.0	40.0	40.0	40.0
6	39.33281453289722	40.0	40.0	40.0	40.0	40.0
7	39.4016852907917	40.0	40.0	40.0	40.0	40.0
8	39.50525893410829	40.0	40.0	40.0	40.0	40.0
9	39.01348475821062	40.0	40.0	40.0	40.0	40.0
10-14	39.598895927821886	40.0	40.0	40.0	40.0	40.0
15-19	39.48964141972982	40.0	40.0	40.0	40.0	40.0
20-24	39.476227185953476	40.0	40.0	40.0	40.0	40.0
25-29	39.62235381379118	40.0	40.0	40.0	40.0	40.0
30-34	39.52425189382425	40.0	40.0	40.0	40.0	40.0
35-39	39.63658029450032	40.0	40.0	40.0	40.0	40.0
40-44	39.54542867912598	40.0	40.0	40.0	40.0	40.0
45-49	39.495098551817215	40.0	40.0	40.0	40.0	40.0
50-54	39.56518384373286	40.0	40.0	40.0	40.0	40.0
55-59	39.47777068475994	40.0	40.0	40.0	40.0	40.0
60-64	39.47923468364127	40.0	40.0	40.0	40.0	40.0
65-69	39.2823000832923	40.0	40.0	40.0	40.0	40.0
70-74	39.45091773517914	40.0	40.0	40.0	40.0	40.0
75-79	39.46406940619281	40.0	40.0	40.0	40.0	40.0
80-84	39.51373350113387	40.0	40.0	40.0	40.0	40.0
85-89	39.59854816057782	40.0	40.0	40.0	40.0	40.0
90-94	39.487580936399944	40.0	40.0	40.0	40.0	40.0
95-99	39.38407962013849	40.0	40.0	40.0	40.0	40.0
100-104	39.286148584669704	40.0	40.0	40.0	40.0	40.0
105-109	39.361108949416334	40.0	40.0	40.0	40.0	40.0
110-114	39.367611867704284	40.0	40.0	40.0	40.0	40.0
115-119	39.25305447470818	40.0	40.0	40.0	40.0	40.0
120-124	39.16845817120623	40.0	40.0	40.0	40.0	40.0
125-129	39.29911478599222	40.0	40.0	40.0	40.0	40.0
130-134	39.307636186770424	40.0	40.0	40.0	40.0	40.0
135-139	38.89749027237354	40.0	40.0	40.0	40.0	40.0
140-144	39.39808365758755	40.0	40.0	40.0	40.0	40.0
145-149	38.99767996108949	40.0	40.0	40.0	40.0	40.0
150-154	39.161702334630355	40.0	40.0	40.0	40.0	40.0
155-159	39.26900217581856	40.0	40.0	40.0	40.0	40.0
160-164	39.23548173323039	40.0	40.0	40.0	40.0	40.0
165-169	39.26097313334833	40.0	40.0	40.0	40.0	40.0
170-174	39.09054894545808	40.0	40.0	40.0	40.0	40.0
175-179	39.345210026811934	40.0	40.0	40.0	40.0	40.0
180-184	39.27976215808705	40.0	40.0	40.0	40.0	40.0
185-189	39.226634403176085	40.0	40.0	40.0	40.0	40.0
190-194	39.23743699803622	40.0	40.0	40.0	40.0	40.0
195-199	39.190119103350575	40.0	40.0	40.0	40.0	40.0
200-204	39.41689820584999	40.0	40.0	40.0	40.0	40.0
205-209	39.22213534858554	40.0	40.0	40.0	40.0	40.0
210-214	39.322422923291114	40.0	40.0	40.0	40.0	40.0
215-219	39.30781193951812	40.0	40.0	40.0	40.0	40.0
220-224	39.2995628621283	40.0	40.0	40.0	40.0	40.0
225-229	39.34042886933894	40.0	40.0	40.0	40.0	40.0
230-234	39.164080520917565	40.0	40.0	40.0	40.0	40.0
235-239	39.224713185269856	40.0	40.0	40.0	40.0	40.0
240-244	39.2655743286377	40.0	40.0	40.0	40.0	40.0
245-249	39.16616710947902	40.0	40.0	40.0	40.0	40.0
250-254	39.26161029675521	40.0	40.0	40.0	40.0	40.0
255-259	38.91756151241192	40.0	40.0	40.0	40.0	40.0
260-264	39.08552945968786	40.0	40.0	40.0	40.0	40.0
265-269	38.75285233981238	40.0	40.0	40.0	36.8	40.0
270-274	38.132089810857316	40.0	36.8	40.0	33.6	40.0
275-279	38.836709853537535	40.0	40.0	40.0	40.0	40.0
280-284	38.471201794757995	40.0	40.0	40.0	36.8	40.0
285-289	38.975932489861925	40.0	40.0	40.0	40.0	40.0
290-294	38.90593579493599	40.0	40.0	40.0	40.0	40.0
295-299	38.9412643574761	40.0	40.0	40.0	40.0	40.0
300-301	38.60164353609776	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.031946541880685686
1101	2	-0.14794128506188997
1101	3	0.2624459430382302
1101	4	0.020771023184160242
1101	5	0.3336869533223492
1101	6	0.29019162248704333
1101	7	0.054397199344670355
1101	8	0.28581260087648985
1101	9	-0.133093455288531
1101	10-14	0.04410397633596119
1101	15-19	0.08635410074142413
1101	20-24	2.3785715371360538E-4
1101	25-29	-0.013501773787346849
1101	30-34	0.0317186639774647
1101	35-39	0.10927077968473498
1101	40-44	0.14946701268690532
1101	45-49	0.021194630726405705
1101	50-54	-0.005494058045094619
1101	55-59	0.010886020943630115
1101	60-64	0.05117720975606943
1101	65-69	0.022205316959791332
1101	70-74	0.04559928312212236
1101	75-79	0.08180473663098553
1101	80-84	0.02994770090499088
1101	85-89	0.07009569799914317
1101	90-94	0.01879600961587613
1101	95-99	0.22005462063292924
1101	100-104	-0.07594110782921604
1101	105-109	0.019376278765143695
1101	110-114	0.04017135377677761
1101	115-119	0.06815580650896891
1101	120-124	-0.1127948190304906
1101	125-129	0.016232808773516183
1101	130-134	-0.0060165820437418915
1101	135-139	0.10739974864893753
1101	140-144	-0.016931308652175403
1101	145-149	-0.05168710261881415
1101	150-154	0.06542601083271649
1101	155-159	-0.013850278261443805
1101	160-164	-0.11796521538116878
1101	165-169	-0.006567519337750127
1101	170-174	0.061815041435536955
1101	175-179	0.0012554592578126744
1101	180-184	-0.0389925878825963
1101	185-189	-6.688300875978825E-4
1101	190-194	0.056577127325475374
1101	195-199	0.021430181989892105
1101	200-204	0.0215442815343323
1101	205-209	0.04260517984311463
1101	210-214	-0.008346257870762486
1101	215-219	-0.05986861279259159
1101	220-224	0.024731007065454946
1101	225-229	0.04314124776072248
1101	230-234	0.04621152270631512
1101	235-239	-0.057111000386001365
1101	240-244	0.024493678529942997
1101	245-249	-0.04462800967029068
1101	250-254	0.05211060172329951
1101	255-259	0.08433136362894089
1101	260-264	0.0402835239870285
1101	265-269	-0.08240236310772531
1101	270-274	0.10188005865509098
1101	275-279	0.021113811276876504
1101	280-284	-0.10605560613907272
1101	285-289	0.025453932045678584
1101	290-294	-0.10297760390128019
1101	295-299	-0.07647894663352162
1101	300-301	0.13600522043590502
1102	1	0.08547741529023511
1102	2	-0.04118351989610858
1102	3	-0.021775897324886273
1102	4	0.0909570311666883
1102	5	0.2721817742631387
1102	6	0.03565438323710168
1102	7	0.051152157617814
1102	8	0.0024944430195859013
1102	9	0.10531442044143802
1102	10-14	0.08665920708306629
1102	15-19	0.057263739336882225
1102	20-24	0.03591140869442455
1102	25-29	0.05111152540686703
1102	30-34	-0.01815091820629533
1102	35-39	-0.01682579111400173
1102	40-44	-0.07183387935726415
1102	45-49	-0.011118864950297791
1102	50-54	-0.10437609324763741
1102	55-59	0.030274118349652213
1102	60-64	-0.019611253230600312
1102	65-69	0.09116176904750972
1102	70-74	-0.0862479945343324
1102	75-79	0.09856941633714911
1102	80-84	0.0808825330733427
1102	85-89	0.013396466813887287
1102	90-94	-0.004661397499930331
1102	95-99	0.05913067373111858
1102	100-104	0.007307220015853488
1102	105-109	-0.04789603774280238
1102	110-114	-0.03722768366443319
1102	115-119	-0.0038649508043562264
1102	120-124	0.05132213939327812
1102	125-129	0.0956528089104367
1102	130-134	0.013886340547628606
1102	135-139	0.03074282623555291
1102	140-144	-0.0216852263212175
1102	145-149	-0.07806093964104122
1102	150-154	0.033142637906223626
1102	155-159	-0.032693427731906866
1102	160-164	-0.15686737805770434
1102	165-169	0.08584086300210458
1102	170-174	0.15660038260990916
1102	175-179	-0.05267521734023717
1102	180-184	-0.04900429467870282
1102	185-189	0.030828612901679264
1102	190-194	-0.05257402726584104
1102	195-199	1.470897526587578E-4
1102	200-204	0.01850462219525184
1102	205-209	-0.11565701131312522
1102	210-214	0.0977570220859647
1102	215-219	-0.06383660052441797
1102	220-224	-0.09765498859412958
1102	225-229	0.026488843759878478
1102	230-234	-0.06185795130937777
1102	235-239	-0.16091673603148138
1102	240-244	-0.15292384332616393
1102	245-249	-0.08805680017341189
1102	250-254	0.023116085433052547
1102	255-259	-0.14819436051512724
1102	260-264	-0.002989175901376484
1102	265-269	-0.11333021227489581
1102	270-274	-0.0293647639972221
1102	275-279	-0.031058792081815056
1102	280-284	-0.11337817287123642
1102	285-289	-0.18925281613498157
1102	290-294	-0.23627898886317666
1102	295-299	-0.18207561670040917
1102	300-301	-0.06889542794485948
1103	1	0.0834611027617953
1103	2	-0.060828564878079305
1103	3	-0.10129422266530952
1103	4	-0.08617042358879701
1103	5	0.015563998507893473
1103	6	-0.0735485432164964
1103	7	-0.16257697039702634
1103	8	0.14461647193406435
1103	9	-0.07647575703607146
1103	10-14	0.030045631978929066
1103	15-19	0.026884630575032986
1103	20-24	-0.12332407280520385
1103	25-29	0.027453458406476727
1103	30-34	-0.012656466566291158
1103	35-39	-0.058020176243694266
1103	40-44	-0.039824917968253715
1103	45-49	0.0074816181245864755
1103	50-54	-0.003210412567135279
1103	55-59	-0.2030748507405562
1103	60-64	-0.13716227363875788
1103	65-69	-0.07011522888717536
1103	70-74	-0.08621718975958714
1103	75-79	-0.07641290592279404
1103	80-84	-0.04964569880259262
1103	85-89	-0.02923174055825939
1103	90-94	-0.0222873295194006
1103	95-99	-0.08136430299028063
1103	100-104	-0.04672715531233251
1103	105-109	-0.06107488046342269
1103	110-114	-0.09355001362557402
1103	115-119	0.01669023524948443
1103	120-124	0.028028128821631526
1103	125-129	-0.0410699831223269
1103	130-134	0.04777719362985522
1103	135-139	-0.09575558101757053
1103	140-144	0.028635773433741463
1103	145-149	-0.05180406982622543
1103	150-154	-0.1187305024305303
1103	155-159	0.007610419364397103
1103	160-164	-0.0014992960049937665
1103	165-169	-0.026128892309969842
1103	170-174	0.014187108552434324
1103	175-179	0.021869537096698366
1103	180-184	-0.016323229113538673
1103	185-189	-0.04310564690859309
1103	190-194	0.012558468270967182
1103	195-199	0.002776697341836609
1103	200-204	0.08616030882667758
1103	205-209	-0.04572177135092659
1103	210-214	-0.026537443556208018
1103	215-219	0.04474462956624592
1103	220-224	-0.0036920243346614257
1103	225-229	0.014645808089348122
1103	230-234	0.05036664350281228
1103	235-239	0.04136981838444598
1103	240-244	-0.011944391511136132
1103	245-249	0.03983902367929204
1103	250-254	-0.006968977751746763
1103	255-259	-0.0630751670401466
1103	260-264	-0.007444666502429698
1103	265-269	-0.09349753821042128
1103	270-274	-0.048617748209771605
1103	275-279	0.03378525874606453
1103	280-284	-0.1252772172231147
1103	285-289	0.09694874510701368
1103	290-294	0.05061147915936459
1103	295-299	0.005820438536609629
1103	300-301	-0.43520319201547863
1104	1	0.11565493413407069
1104	2	0.02918048615582336
1104	3	0.026283581414617174
1104	4	0.23479316340556267
1104	5	0.023723853691350882
1104	6	0.19425066307745453
1104	7	0.34222007757345096
1104	8	0.23415208427132228
1104	9	0.1916297919439458
1104	10-14	0.04115194681565981
1104	15-19	0.14834672066762522
1104	20-24	0.07699062468138607
1104	25-29	0.14147977602814876
1104	30-34	0.10847143150515137
1104	35-39	0.06794236640060802
1104	40-44	0.11404265844335981
1104	45-49	0.09647138349393458
1104	50-54	0.1243952408478961
1104	55-59	0.06254653754879769
1104	60-64	0.08660156399962204
1104	65-69	0.08124590736569814
1104	70-74	0.13268415397082833
1104	75-79	0.04047632334685147
1104	80-84	0.03880378946588081
1104	85-89	0.09223591940136089
1104	90-94	0.169349515150941
1104	95-99	0.12116163170303906
1104	100-104	-0.01247247314286426
1104	105-109	0.14188550385739518
1104	110-114	0.17744072647050757
1104	115-119	0.10800820503294517
1104	120-124	0.1897882067997685
1104	125-129	0.17859443238975103
1104	130-134	0.24047788290090466
1104	135-139	0.06902336488509064
1104	140-144	0.26498751053602376
1104	145-149	0.2892723069752847
1104	150-154	0.2440237968105805
1104	155-159	0.16769953723670739
1104	160-164	0.15804954476642763
1104	165-169	0.22664281276556864
1104	170-174	0.29797740305915
1104	175-179	0.2034694813980309
1104	180-184	0.179457596619244
1104	185-189	0.2517296938976443
1104	190-194	0.24993506090481787
1104	195-199	0.19855195320761254
1104	200-204	0.2887029531210459
1104	205-209	0.14445019829329198
1104	210-214	0.19681979378975
1104	215-219	0.19990998499338986
1104	220-224	0.24613322108760372
1104	225-229	0.19074272377548596
1104	230-234	0.17757683635945654
1104	235-239	0.2499000697247027
1104	240-244	0.2045674792679506
1104	245-249	0.09264136302343928
1104	250-254	0.19233200393730954
1104	255-259	0.28064132672856346
1104	260-264	0.10375215867336607
1104	265-269	0.21280058892179454
1104	270-274	0.13140035385804083
1104	275-279	0.14805108064957295
1104	280-284	0.2408078624956147
1104	285-289	0.2660443379497295
1104	290-294	0.21879361381090234
1104	295-299	0.15380122283484354
1104	300-301	0.10042633834418524
1201	1	-0.06748229346462153
1201	2	-0.10611158374600649
1201	3	-0.10884139247663427
1201	4	-0.010698725475592141
1201	5	0.0683941871871383
1201	6	-0.0735485432164964
1201	7	0.06383812394259536
1201	8	-0.059157112971597314
1201	9	0.014090280699775803
1201	10-14	0.08740412254496732
1201	15-19	0.11443180038634893
1201	20-24	0.06988347436460174
1201	25-29	0.17084968482157592
1201	30-34	0.0764001372073011
1201	35-39	0.064243974699707
1201	40-44	0.0748920631638228
1201	45-49	0.0920099200113711
1201	50-54	0.1054688327158928
1201	55-59	0.07466099831604112
1201	60-64	0.09076225466312593
1201	65-69	0.08837533715055912
1201	70-74	0.13566960269324113
1201	75-79	0.07302105634134648
1201	80-84	0.03488260308420621
1201	85-89	0.10963618397003927
1201	90-94	0.09242965161267591
1201	95-99	0.08165456493426149
1201	100-104	0.037801146574466316
1201	105-109	0.13364210066864501
1201	110-114	0.11173300524234975
1201	115-119	0.006124197513635465
1201	120-124	0.08085831750087635
1201	125-129	0.07817529989653593
1201	130-134	0.1413620992902409
1201	135-139	0.005376494454125691
1201	140-144	0.0829753960752484
1201	145-149	0.05989404338132687
1201	150-154	0.04428836549399762
1201	155-159	-0.028615995729943222
1201	160-164	-0.03319740921254066
1201	165-169	0.08255035297304403
1201	170-174	-0.05373741974946
1201	175-179	0.03545444275707865
1201	180-184	-0.010285493264483136
1201	185-189	-0.06725659030482234
1201	190-194	0.0638792229879499
1201	195-199	-0.10439311397892226
1201	200-204	0.06804710127950386
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2404	185-189	0.07622807950649957
2404	190-194	0.17781790223322957
2404	195-199	0.18487575394561162
2404	200-204	0.07704474278894224
2404	205-209	0.12339969091322445
2404	210-214	0.1102019432362411
2404	215-219	0.1296090163586996
2404	220-224	0.17539406057099427
2404	225-229	0.18502316657991003
2404	230-234	0.14446452086130535
2404	235-239	0.12196533725237657
2404	240-244	0.15050843867754793
2404	245-249	0.21849468405664396
2404	250-254	0.15625036187089592
2404	255-259	0.27551563484664854
2404	260-264	0.20504943727115688
2404	265-269	0.2748220372612806
2404	270-274	0.12334569518645111
2404	275-279	0.20937488138757487
2404	280-284	0.26373221673915737
2404	285-289	0.24641218850323554
2404	290-294	0.2249628942537072
2404	295-299	0.3137943237812948
2404	300-301	0.2495360384925931
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	10.0
18	20.0
19	47.0
20	44.0
21	56.0
22	113.0
23	203.0
24	385.0
25	376.0
26	360.0
27	316.0
28	364.0
29	481.0
30	512.0
31	659.0
32	703.0
33	877.0
34	1146.0
35	1439.0
36	2085.0
37	3097.0
38	6446.0
39	126487.0
40	18255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.07113240354567674	0.006079692610741601	0.09484320472756898	99.82794469911602
2	0.009119705251126283	0.015199508751877141	0.1605068124198226	99.81517397357717
3	0.009727508177186562	0.13436120669738938	99.61272358069576	0.24318770442966403
4	0.07660412689534417	99.47106674286547	0.10761055921012634	0.34471857102904874
5	0.19941391763232452	0.14104886856920515	0.08085991172286329	99.5786773020756
6	99.77322746561934	0.06566068019600929	0.013983293004705681	0.14712856117994674
7	99.91002054936102	0.05654114127989689	0.020670954876521444	0.012767354482557362
8	99.75255651074282	0.10396274364368138	0.09058741990004986	0.05289332571345193
9	28.820174851959486	21.638233970890433	26.31838134263932	23.223209834510765
10-14	59.83511873639669	10.242214953611946	9.766175022190877	20.15649128780049
15-19	39.73164236816187	20.34362422635912	19.934460913656203	19.990272491822815
20-24	59.92680050096667	39.83755061344099	0.2023321700854805	0.03331671550686397
25-29	19.501829987475833	19.69723130798507	59.686287861285734	1.114650843253365
30-34	0.42594326430855656	59.60688707578945	0.22239515570092777	39.74477450420107
35-39	59.853236220376694	0.5469291472623145	19.932393818168553	19.667440814192435
40-44	39.65345752118773	49.61600661470556	0.06857893264916526	10.661956931457546
45-49	20.097275081771866	19.925827750148954	20.537323232937343	39.439573935141844
50-54	40.28078554778838	20.068895327982375	19.736213334760038	19.914105789469204
55-59	20.063715565931627	29.734741398702585	10.235346331795162	39.96619670357063
60-64	39.98431429770004	0.0669986199013868	0.3402216669402545	59.608465415458326
65-69	30.07459828186842	20.056176701260327	40.63715565931628	9.232069357554975
70-74	39.985043865248876	51.194362874739326	8.687568776940802	0.13302448307099302
75-79	40.340586450714675	19.819188842480287	1.0144636766556625	38.82576103014938
80-84	40.06395875511457	0.10700324049586274	39.740881925571955	20.088156078817615
85-89	39.23979061411348	0.5322195268754446	50.230117764361836	9.99787209464923
90-94	20.057635836358	59.663304576212454	10.363750220390196	9.915309367039354
95-99	9.856214395583685	10.040551796256104	59.674491278627926	20.428742529532286
100-104	21.355153994219364	20.209970561806223	10.360031177004892	48.07484426696952
105-109	60.73188229571984	38.73467898832685	0.38910505836575876	0.14433365758754862
110-114	40.97993677042802	49.458049610894946	9.35420719844358	0.20780642023346305
115-119	39.58900778210116	31.106882295719846	19.758268482490273	9.545841439688715
120-124	20.945768482490273	30.137889105058363	39.34922178988327	9.567120622568092
125-129	27.968020428015567	40.50413424124514	20.36247568093385	11.165369649805447
130-134	26.608706225680933	31.433244163424124	30.855179961089497	11.102869649805447
135-139	9.561162451361868	11.05179961089494	21.910992217898833	57.47604571984436
140-144	39.76313229571984	19.896643968871594	20.03125	20.30897373540856
145-149	0.6397130350194552	29.755836575875488	30.385700389105057	39.21875
150-154	39.004863813229576	10.874270428015564	10.562864785992218	39.55800097276265
155-159	29.522287843248833	60.53886252293594	0.05155648292373896	9.887293150891477
160-164	29.57265061193222	10.663124167826895	0.4314228564132807	59.3328023638276
165-169	30.52608539691997	29.511001404434612	29.178436152943537	10.784477045701884
170-174	9.795293016129719	29.440962068105957	20.10809890624335	40.65564600952097
175-179	67.45858133865113	0.48200682153952784	10.870445467202499	21.18896637260684
180-184	30.89500787334553	31.167747858389216	8.62444445795512	29.312799810310132
185-189	30.73109637096529	9.4194395637133	28.039324169042857	31.81013989627855
190-194	29.324837821241616	30.518424844509024	21.518491722347534	18.638245611901823
195-199	20.898985157965647	39.27910466272857	19.920865181409397	19.901044997896385
200-204	70.32581666960523	0.21558983213662533	19.865028362283333	9.593565135974805
205-209	20.3538445637437	48.879309820706595	9.56000462065066	21.206840994899046
210-214	47.818262513755556	20.750734136272715	30.831413128727682	0.5995902212440494
215-219	40.064324321037944	20.089129919321007	19.381805580043654	20.464740179597396
220-224	28.41833911927967	20.669994345782744	39.70987177694417	11.201794757993422
225-229	32.47052815253011	1.0488876999495376	46.009520972282175	20.47106317523818
230-234	20.130229390986084	50.24994072191586	20.15430541284906	9.465524474248992
235-239	49.3335927382827	28.900832325099252	1.03101307765733	20.734561858960717
240-244	30.879078788173565	38.54194152444993	9.97537679582196	20.603602891554544
245-249	49.40922549383204	9.777175201697482	20.384243581247453	20.429355723223026
250-254	29.079578548021328	1.214501547309991	50.51331780956839	19.192602095100288
255-259	10.767818384109825	39.83013029018902	11.009186583089635	38.39286474261152
260-264	39.94394421172308	30.003100699785385	29.52413377999623	0.5288213084953094
265-269	20.382541236267244	50.11387471956906	11.556976878507287	17.946607165656406
270-274	10.177955848466977	10.171389660686167	37.63009259540732	42.02056189543954
275-279	39.66877230527909	19.775655250822293	19.865271554423362	20.690300889475253
280-284	18.12340785145824	47.87723660771284	2.2264240419749632	31.772931498853957
285-289	41.00900418898461	20.63594744617854	28.82094370709939	9.534104657737462
290-294	29.256978179319905	20.399691142226573	20.434589638672882	29.90874103978064
295-299	38.60879212044614	29.49164129394205	21.03002516795872	10.86954141765309
300-301	46.12397539419657	29.066292186283636	24.801489780654222	0.008242638865568665
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.9166666666666665
46	1.1666666666666665
47	0.8333333333333333
48	1.5
49	14.083333333333334
50	823.5000000000001
51	598.5833333333333
52	80.91666666666664
53	357.7499999999997
54	13842.083333333347
55	34267.333333333285
56	4159.250000000006
57	185.00000000000006
58	47.166666666666664
59	16.333333333333336
60	5.25
61	2.833333333333333
62	1.3333333333333333
63	0.0
64	0.25
65	0.0
66	0.0
67	0.25
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.25
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018239077832224802
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	1.2159607001501712E-4
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	2.431945057497261E-4
295-299	0.0
300-301	3.0528198897321454E-4
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	1.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	1.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	1.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	7.0
295-299	391.0
300-302	164081.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	2.1813937087340864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	52.56410256410257	1.146630026385866
2	11.17614269788183	0.4875913473814764
3	6.0200668896321075	0.39396408117605575
4	4.570791527313267	0.39882783526464904
5	2.7591973244147154	0.30094478423170923
6	2.9264214046822743	0.38302063447672086
7	1.9230769230769231	0.29364915309881934
8	1.8673355629877368	0.3258715239357498
9	1.560758082497213	0.3064165075813767
>10	13.322185061315498	5.053440498048419
>50	0.2508361204013378	0.32769543171897225
>100	0.6131549609810479	2.7668681071485026
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.36231884057971014	68.1521382278912
>10k+	0.08361204013377926	19.662941841660487
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	11206	6.8129035395970385	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10670	6.487032015661288	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	10466	6.363006286402159	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	9965	6.0584136866040055	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9858	5.99336097566907	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9534	5.796378935081043	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	9397	5.713087146313883	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	9334	5.67478508286621	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	9293	5.64985834316217	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8636	5.250422538636446	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	8616	5.238263153414963	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8582	5.217592198538442	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	8383	5.096606315584684	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	7595	4.617526537858246	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	6741	4.098320788900913	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	6164	3.747522525261123	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	383	0.2328522269914033	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	337	0.20488564098199197	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	332	0.20184579467662114	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	323	0.1963740713269537	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	322	0.19576610206587952	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	305	0.18543062462761883	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	270	0.16415170049002323	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	220	0.13375323743631523	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	213	0.12949745260879608	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	199	0.12098588295375787	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	188	0.1142982210819421	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	168	0.1021388358604589	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	167	0.10153086659938475	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	166	0.10092289733831057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
