##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DPFS2_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	132107
Sequences flagged as poor quality	0
Sequence length	38-301
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.127313465599855	34.0	34.0	34.0	20.0	34.0
2	33.01946149711976	34.0	34.0	34.0	34.0	34.0
3	33.55945559281491	34.0	34.0	34.0	34.0	34.0
4	33.36627128009871	34.0	34.0	34.0	34.0	34.0
5	33.11377141256708	34.0	34.0	34.0	34.0	34.0
6	33.02430605494031	34.0	34.0	34.0	34.0	34.0
7	33.480156236989714	34.0	34.0	34.0	34.0	34.0
8	33.425473290590205	34.0	34.0	34.0	34.0	34.0
9	33.33212471708539	34.0	34.0	34.0	34.0	34.0
10-14	33.2533779436366	34.0	34.0	34.0	34.0	34.0
15-19	33.44714511721559	34.0	34.0	34.0	34.0	34.0
20-24	33.66890777930011	34.0	34.0	34.0	34.0	34.0
25-29	33.5947194319756	34.0	34.0	34.0	34.0	34.0
30-34	33.51410901769021	34.0	34.0	34.0	34.0	34.0
35-39	33.551433765068865	34.0	34.0	34.0	34.0	34.0
40-44	33.37425022027663	34.0	34.0	34.0	34.0	34.0
45-49	33.568405566159996	34.0	34.0	34.0	34.0	34.0
50-54	33.5172512472651	34.0	34.0	34.0	34.0	34.0
55-59	33.4069438030419	34.0	34.0	34.0	34.0	34.0
60-64	33.31932330349047	34.0	34.0	34.0	34.0	34.0
65-69	33.50352308046846	34.0	34.0	34.0	34.0	34.0
70-74	33.4769454289195	34.0	34.0	34.0	34.0	34.0
75-79	33.34039530115452	34.0	34.0	34.0	34.0	34.0
80-84	33.57355113175795	34.0	34.0	34.0	34.0	34.0
85-89	33.5326938300056	34.0	34.0	34.0	34.0	34.0
90-94	33.07082158984149	34.0	34.0	34.0	34.0	34.0
95-99	33.26652239511503	34.0	34.0	34.0	34.0	34.0
100-104	33.24720345217587	34.0	34.0	34.0	34.0	34.0
105-109	33.35515245654284	34.0	34.0	34.0	34.0	34.0
110-114	33.19877219010834	34.0	34.0	34.0	34.0	34.0
115-119	33.026365165559284	34.0	34.0	34.0	34.0	34.0
120-124	33.136967490707775	34.0	34.0	34.0	34.0	34.0
125-129	33.2305448554839	34.0	34.0	34.0	34.0	34.0
130-134	32.93483630017754	34.0	34.0	34.0	31.2	34.0
135-139	33.07621773183524	34.0	34.0	34.0	34.0	34.0
140-144	33.18566015656621	34.0	34.0	34.0	34.0	34.0
145-149	33.271294071927684	34.0	34.0	34.0	34.0	34.0
150-154	32.94788239616203	34.0	34.0	34.0	34.0	34.0
155-159	33.05715895263218	34.0	34.0	34.0	34.0	34.0
160-164	33.20966238137518	34.0	34.0	34.0	34.0	34.0
165-169	33.24184448762135	34.0	34.0	34.0	34.0	34.0
170-174	32.84406046706556	34.0	34.0	34.0	31.2	34.0
175-179	33.19628466399137	34.0	34.0	34.0	34.0	34.0
180-184	33.0105522459002	34.0	34.0	34.0	34.0	34.0
185-189	33.14272399683063	34.0	34.0	34.0	34.0	34.0
190-194	33.08539999546737	34.0	34.0	34.0	34.0	34.0
195-199	32.492188339030534	34.0	34.0	34.0	28.4	34.0
200-204	32.91862949731014	34.0	34.0	34.0	34.0	34.0
205-209	32.48702308514847	34.0	34.0	34.0	31.2	34.0
210-214	32.560475250521854	34.0	34.0	34.0	31.2	34.0
215-219	32.89064620336781	34.0	34.0	34.0	34.0	34.0
220-224	32.8466937003619	34.0	34.0	34.0	34.0	34.0
225-229	32.99889194242603	34.0	34.0	34.0	34.0	34.0
230-234	32.65654718964047	34.0	34.0	34.0	31.2	34.0
235-239	32.76303355628572	34.0	34.0	34.0	34.0	34.0
240-244	32.58119827350521	34.0	34.0	34.0	31.2	34.0
245-249	32.79758427028602	34.0	34.0	34.0	34.0	34.0
250-254	32.399106120603605	34.0	34.0	34.0	31.2	34.0
255-259	32.30073430668063	34.0	34.0	34.0	31.2	34.0
260-264	32.658389505855	34.0	34.0	34.0	34.0	34.0
265-269	32.71418221126696	34.0	34.0	34.0	34.0	34.0
270-274	32.36050270659016	34.0	34.0	34.0	31.2	34.0
275-279	32.46119379221721	34.0	34.0	34.0	31.2	34.0
280-284	31.92300533383197	34.0	34.0	34.0	22.8	34.0
285-289	31.71058029801382	34.0	34.0	34.0	20.0	34.0
290-294	31.74716135959481	34.0	34.0	34.0	20.0	34.0
295-299	31.87113899890225	34.0	34.0	34.0	22.8	34.0
300-301	31.722210451158805	34.0	34.0	34.0	20.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.09834770504194523
1101	2	0.371342975543385
1101	3	0.13720554222436476
1101	4	0.09870087029700159
1101	5	0.05529336627640902
1101	6	0.02845492712636144
1101	7	-0.28110722760632
1101	8	-0.09226487321375743
1101	9	0.09771975789070098
1101	10-14	0.1034045813286113
1101	15-19	0.05035000325831618
1101	20-24	0.0024539765228794863
1101	25-29	0.03515938434698995
1101	30-34	0.04211730544982828
1101	35-39	-0.0010026152457243143
1101	40-44	0.0594689044446568
1101	45-49	0.06081181715450157
1101	50-54	0.04398267538721967
1101	55-59	-0.15628622511183465
1101	60-64	0.03142138404889039
1101	65-69	-0.06156654554374086
1101	70-74	-0.25974030740884757
1101	75-79	-6.538112843728072E-4
1101	80-84	-0.0301488261803442
1101	85-89	-2.681542486300259E-4
1101	90-94	-0.2724853178846516
1101	95-99	-0.10686874346390596
1101	100-104	0.14760687177692944
1101	105-109	-0.1651271447630691
1101	110-114	-0.1313409452180423
1101	115-119	0.0359566388585435
1101	120-124	-0.1406530023065713
1101	125-129	0.01739175813069238
1101	130-134	0.009472041569971168
1101	135-139	-0.05065557113717034
1101	140-144	0.04147450883346693
1101	145-149	-0.006819665505744865
1101	150-154	0.022757983665186998
1101	155-159	-0.16373829111437033
1101	160-164	-0.1142423206977341
1101	165-169	-0.07072372019392503
1101	170-174	-0.13629298882127472
1101	175-179	-0.03938961278068831
1101	180-184	-0.1470474203033234
1101	185-189	-0.17001634501063023
1101	190-194	-0.1847214832497528
1101	195-199	-0.18404155533053768
1101	200-204	-0.09232571135972734
1101	205-209	-0.1502336152803352
1101	210-214	-0.11583411969127866
1101	215-219	-0.2502460458126805
1101	220-224	-0.060490290852740713
1101	225-229	-0.10222975892210684
1101	230-234	-0.3630392230311301
1101	235-239	-0.2692539595016967
1101	240-244	-0.2870992332478437
1101	245-249	-0.2276076548957633
1101	250-254	-0.40072083063055075
1101	255-259	-0.3636158147473303
1101	260-264	-0.2210973906120941
1101	265-269	-0.15012919500794908
1101	270-274	-0.3756590344575095
1101	275-279	-0.32955296767742226
1101	280-284	-0.6809795210371803
1101	285-289	-0.589586948818134
1101	290-294	-0.48528862925692096
1101	295-299	-0.4725004417590206
1101	300-301	-0.5419226570359363
1102	1	0.21259136614769147
1102	2	-0.2614988640182716
1102	3	0.021196594780334976
1102	4	0.0486280184018284
1102	5	0.17770507183162465
1102	6	-0.4242221235005772
1102	7	0.010223396867814927
1102	8	-0.14113207754792967
1102	9	-0.02168552438474336
1102	10-14	0.0680662817534099
1102	15-19	-0.08929168854738379
1102	20-24	-0.014096742761317671
1102	25-29	0.04180840238553429
1102	30-34	-0.053407946453553734
1102	35-39	0.07566220447147742
1102	40-44	-0.033083784918957804
1102	45-49	-0.0486271206443476
1102	50-54	0.08118741999515322
1102	55-59	-0.172488076165628
1102	60-64	-0.19137612872584242
1102	65-69	-0.10557832497692488
1102	70-74	-0.01058579982610297
1102	75-79	-0.06695519239212899
1102	80-84	-0.19163377354228572
1102	85-89	-0.09462826778681688
1102	90-94	-0.16982580906075384
1102	95-99	-0.12382542617402237
1102	100-104	0.01276792323935183
1102	105-109	-0.2549889243508616
1102	110-114	-0.1849136960510691
1102	115-119	-0.3090945767406268
1102	120-124	-0.02431954556215743
1102	125-129	0.010906505755023943
1102	130-134	-0.12159052784875968
1102	135-139	-0.18588326458404225
1102	140-144	-0.23460016306108145
1102	145-149	-0.09992719726176347
1102	150-154	-0.15172326804589886
1102	155-159	-0.2327229030023119
1102	160-164	-0.22282588088390298
1102	165-169	-0.14382319279337707
1102	170-174	-0.4147470188001918
1102	175-179	-0.05634292464583268
1102	180-184	-0.23690065902297874
1102	185-189	-0.25167079168632256
1102	190-194	-0.1825097476519204
1102	195-199	-0.29638100487182584
1102	200-204	-0.036083009107855446
1102	205-209	-0.2098624726614915
1102	210-214	-0.1540280484218428
1102	215-219	-0.16313197434521243
1102	220-224	-0.11922054932536241
1102	225-229	-0.27337256818858435
1102	230-234	-0.5699262407513785
1102	235-239	-0.28832102832998174
1102	240-244	-0.495649356828757
1102	245-249	-0.26937381467122634
1102	250-254	-0.3137868940547932
1102	255-259	-0.5102937922095236
1102	260-264	-0.4357951410205416
1102	265-269	-0.48993272230048035
1102	270-274	-0.5251835621270686
1102	275-279	-0.5008256416332628
1102	280-284	-0.6824583028226137
1102	285-289	-0.9490063279811345
1102	290-294	-1.4256158044904659
1102	295-299	-1.087396541082537
1102	300-301	-0.8379532356586559
1103	1	0.017208483264809615
1103	2	0.5282283632089531
1103	3	-0.17087047228672958
1103	4	0.1800093997832093
1103	5	-0.060658291531744624
1103	6	0.16730690552845573
1103	7	0.17470037384478587
1103	8	-0.012691136606989062
1103	9	0.02323439553517659
1103	10-14	-0.17306234687521993
1103	15-19	-6.655624212612565E-4
1103	20-24	0.1207130670484915
1103	25-29	0.10244403802116864
1103	30-34	-0.03302506357067614
1103	35-39	0.04914819045747265
1103	40-44	0.057919718584543034
1103	45-49	0.004763770246547949
1103	50-54	-0.0033471145393804136
1103	55-59	-0.08251059868814536
1103	60-64	0.02311336076364512
1103	65-69	0.07731707041847358
1103	70-74	-0.02074595998625739
1103	75-79	0.03428354634660735
1103	80-84	0.08230266289415766
1103	85-89	0.14023159207304303
1103	90-94	-0.00392240565735591
1103	95-99	0.016577476728883767
1103	100-104	0.020450605922036402
1103	105-109	0.006496284756153159
1103	110-114	0.051143242558069346
1103	115-119	-0.1402153727105997
1103	120-124	0.03639093289774564
1103	125-129	0.09100203051411881
1103	130-134	0.16613966173757433
1103	135-139	0.1369266683187007
1103	140-144	0.17437217894583767
1103	145-149	0.23686507719023098
1103	150-154	0.0768147129504797
1103	155-159	0.050339724957154885
1103	160-164	0.07084202819268626
1103	165-169	0.14078072342269365
1103	170-174	0.1833185273300728
1103	175-179	0.1935655253173323
1103	180-184	0.0782561824852408
1103	185-189	0.1389762578311604
1103	190-194	0.0599648320319659
1103	195-199	-0.07549386875212605
1103	200-204	0.3969356389107901
1103	205-209	-0.022111889910910065
1103	210-214	-0.0018662832463860468
1103	215-219	0.11281719064504614
1103	220-224	0.12536865142821085
1103	225-229	-0.00674741252232991
1103	230-234	-0.15288118535015371
1103	235-239	-0.09272758068173914
1103	240-244	-0.1166858545775824
1103	245-249	-0.04712391844871888
1103	250-254	-0.2064777648278593
1103	255-259	-0.2944844873591208
1103	260-264	-0.15505624521315298
1103	265-269	-0.06010866507917001
1103	270-274	-0.1597590323189806
1103	275-279	-0.14969047542960112
1103	280-284	-0.12487935209297163
1103	285-289	-0.2875125705969701
1103	290-294	-0.4250985135622223
1103	295-299	-0.45714238250340244
1103	300-301	-0.7106064036848849
1104	1	0.1517805553368845
1104	2	-0.018255620775029513
1104	3	-0.11619079260704979
1104	4	-0.33425486448105346
1104	5	0.02229966642621406
1104	6	0.2334355341570813
1104	7	-0.15869552205110438
1104	8	0.027786841370989634
1104	9	-0.228892731591948
1104	10-14	-0.13103281734569094
1104	15-19	-0.13343583269153214
1104	20-24	-0.01634899501356557
1104	25-29	-0.032966372389239496
1104	30-34	0.005601062555449232
1104	35-39	-0.09731076850148668
1104	40-44	-0.21101171284689002
1104	45-49	-0.09772621974343565
1104	50-54	-0.06295672414898945
1104	55-59	-0.0923078959854493
1104	60-64	-0.19272748007719542
1104	65-69	-0.14431706371566833
1104	70-74	-0.23400922324952234
1104	75-79	-0.19127951671644894
1104	80-84	-0.11325539516390393
1104	85-89	-0.15859223175078085
1104	90-94	-0.15811409734904913
1104	95-99	-0.2517533541019503
1104	100-104	-0.14624108576965966
1104	105-109	-0.232916852278791
1104	110-114	-0.3376163987537737
1104	115-119	-0.36585133349738186
1104	120-124	-0.14053576177837357
1104	125-129	-0.3764808816323679
1104	130-134	-0.35402296028118485
1104	135-139	-0.2345319132326935
1104	140-144	-0.14901457747550495
1104	145-149	-0.27795579018878414
1104	150-154	-0.25346812803003615
1104	155-159	-0.38052555893138873
1104	160-164	-0.2304276135420622
1104	165-169	-0.11519874441724198
1104	170-174	-0.4079888265354228
1104	175-179	-0.3564407600377919
1104	180-184	-0.27740901759856484
1104	185-189	-0.2904371090240616
1104	190-194	-0.20773263287480148
1104	195-199	-0.5382278517186698
1104	200-204	0.16103820301336214
1104	205-209	-0.19274126054028073
1104	210-214	-0.32694660832670763
1104	215-219	-0.4655076376797709
1104	220-224	-0.33277725671769787
1104	225-229	-0.2401626459375663
1104	230-234	-0.5221970046659763
1104	235-239	-0.1206177085718636
1104	240-244	-0.554170892062622
1104	245-249	-0.4252975466223603
1104	250-254	-0.6216481799982674
1104	255-259	-0.6919487348233702
1104	260-264	-0.7034182435751468
1104	265-269	-0.4328674378379347
1104	270-274	-0.7751341976941433
1104	275-279	-0.7031334288725581
1104	280-284	-0.7278057050193283
1104	285-289	-1.0407192738036706
1104	290-294	-0.7696427081064066
1104	295-299	-1.1229746953399236
1104	300-301	-1.5624265580113779
1201	1	-1.0893111245783267
1201	2	-0.30971825988472546
1201	3	0.11325806801828264
1201	4	-0.3665919074063311
1201	5	-0.21572365100886515
1201	6	-0.21397305089855934
1201	7	0.20787575510840384
1201	8	-0.264613794559061
1201	9	-0.1784104846391159
1201	10-14	-0.19510700918458923
1201	15-19	-0.12253811144086768
1201	20-24	-0.06630871944388872
1201	25-29	-0.12687840206597656
1201	30-34	-0.12570153415890672
1201	35-39	-0.19928427141616822
1201	40-44	-0.15073932280979108
1201	45-49	-0.1373658593830811
1201	50-54	-0.17553012107533306
1201	55-59	-0.245834128099915
1201	60-64	-0.1826448091709949
1201	65-69	-0.1251066768582163
1201	70-74	-0.1809376811191541
1201	75-79	-0.46798115953573927
1201	80-84	-0.19307097545006258
1201	85-89	-0.3148381247025611
1201	90-94	-0.4093287237401455
1201	95-99	-0.3313103228353853
1201	100-104	-0.38012237882742284
1201	105-109	-0.4023498146529292
1201	110-114	-0.3933715656854133
1201	115-119	-0.36575488410681345
1201	120-124	-0.39229092871001114
1201	125-129	-0.2644777019821234
1201	130-134	-0.33755025227907254
1201	135-139	-0.3848955632311899
1201	140-144	-0.13631697421185862
1201	145-149	-0.3107961777461554
1201	150-154	-0.49059204439923576
1201	155-159	-0.6981398713570144
1201	160-164	-0.3517687660152973
1201	165-169	-0.40909641567340316
1201	170-174	-0.6864181993044198
1201	175-179	-0.4856095514707235
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2404	280-284	1.1061457882400383
2404	285-289	1.2507909715251948
2404	290-294	1.100721092448321
2404	295-299	1.1325946670184202
2404	300-301	1.1476142059654357
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	15.0
16	40.0
17	51.0
18	100.0
19	194.0
20	351.0
21	454.0
22	536.0
23	452.0
24	505.0
25	502.0
26	691.0
27	735.0
28	995.0
29	1497.0
30	2358.0
31	4262.0
32	12523.0
33	102578.0
34	3268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.3860507013254408	0.06888355651101001	0.11278736175978563	99.43227838040376
2	0.06964210016350754	0.15820868406709865	0.3512384182159511	99.42091079755345
3	0.23011649647634116	0.20816459385195335	99.398215083228	0.16350382644371608
4	0.08856457265701287	99.43227838040376	0.277805112522425	0.2013519344167985
5	0.18167091827079565	0.25661017205749886	0.20665066953303005	99.35506824013868
6	99.2914834187439	0.33760512311989527	0.0749392537867032	0.29597220434950455
7	99.78805059535074	0.06964051867047166	0.04844557820554551	0.09386330777324442
8	99.44968851007138	0.13171141574632683	0.10824558880301574	0.3103544853792759
9	30.33374461610664	23.05479649072343	25.109948753661804	21.501510139508127
10-14	59.67798829736501	10.132695466553628	9.926044797020598	20.263271439060762
15-19	23.85369435381925	36.322980614199096	19.71341412642782	20.109910905553832
20-24	58.06762699932631	39.8993240327916	1.550712679873133	0.4823362880089625
25-29	3.7254649640064494	4.337544566147138	58.562226074318545	33.37476439552787
30-34	1.962651487052162	58.55495923758771	0.7518148167773093	38.730574458582815
35-39	46.6078152037824	14.16371324369865	20.192632313601287	19.035839238917664
40-44	38.58813234196466	46.77207150329825	1.1179392612577423	13.521856893479345
45-49	20.306062656994516	20.009599471877635	33.23128216542282	26.453055705705026
50-54	54.97929426371613	20.29858655906245	19.72715366154638	4.994965515675037
55-59	17.101045507195906	40.23756709491328	3.158627894828487	39.50275950306233
60-64	39.42970932741741	1.2317430511913867	0.9143428255585926	58.424204795832615
65-69	38.30960064572698	20.480327588321792	39.33604001568886	1.8740317502623642
70-74	40.32110779463275	57.32737275853766	1.9335523117671376	0.41796713506244515
75-79	47.597396106276186	20.759206472778164	25.50231581753357	6.141081603412083
80-84	39.334868291500676	0.7081509308208491	39.75627489888968	20.200705878788796
85-89	23.203166898457457	8.92318786567648	41.50039843776039	26.373246798105676
90-94	19.355395945085917	57.99693911567718	3.4151589538442892	19.232505985392613
95-99	18.431141390325063	2.774679815496142	58.51148300127892	20.282695792899872
100-104	37.224466663231816	10.82736527544999	43.9769844613291	7.9711835999890885
105-109	44.129895794814594	42.11686863114759	13.04989603729321	0.7033395367446034
110-114	46.59785312670069	47.005186028496624	1.2450409834201714	5.151919861382506
115-119	26.705139032722236	44.88014786488982	23.209268544898496	5.20544455748945
120-124	51.93876266742687	25.99129296841237	19.575960586352313	2.493983777808442
125-129	3.466034980338156	40.364786722454845	35.59953836751345	20.569639929693555
130-134	33.46923298600051	8.344784699155177	26.493000257845324	31.692982056998986
135-139	9.044007220764248	20.963274222175027	55.288906418288555	14.703812138772165
140-144	40.303673409904455	19.576794275079166	19.67349491600555	20.44603739901083
145-149	16.448097721274483	34.617311996792836	37.95491749756274	10.97967278436994
150-154	7.4782722282471665	29.744339843310946	20.1952081947343	42.582179733707584
155-159	21.83193583667517	74.28955523149835	0.9657917122372098	2.9127172195892577
160-164	21.504391302168077	19.177555667977753	16.21902882413978	43.09902420571439
165-169	38.00465567174475	22.27216230425362	20.898708750049384	18.824473273952247
170-174	2.206083525356129	37.649792980173515	20.594976805267606	39.54914668920274
175-179	41.41702879489604	2.3348528020970494	19.332781905042832	36.91533649796408
180-184	38.288826829238616	38.83610776475742	0.881459983694131	21.993605422309837
185-189	39.39147702631759	1.5574475207900604	4.520782451895642	54.53029300099671
190-194	20.4252876538379	39.08970901932005	37.26535105415977	3.219652272682287
195-199	21.987210343840065	38.2719340470518	19.80523041488694	19.935625194221195
200-204	75.51758556663411	1.7963511808295167	20.650525673440036	2.035537579096335
205-209	21.040728464205433	39.59568712614775	2.4002743170648078	36.963310092582006
210-214	35.05892037745629	9.88322636705947	52.78930438892022	2.2685488665640197
215-219	39.17871759058777	20.216123739761034	4.364559000225668	36.24059966942552
220-224	4.167187704920533	22.43900802449744	69.70805591223377	3.685748358348253
225-229	56.56717851897829	1.6734200264440928	21.04943703362548	20.70996442095214
230-234	20.43995998145482	57.028714526243874	20.487543007735294	2.0437824845660186
235-239	55.106255443179684	21.024286304769255	1.9794489938792352	21.890009258171826
240-244	39.072771922331874	22.00210490993121	8.157898750781714	30.7672244169552
245-249	55.59719762594438	9.974724941159602	14.00348084998635	20.42459658290967
250-254	15.788309554717259	34.09217448872035	37.97129481467018	12.148221141892211
255-259	22.408427758150435	35.8294129491662	19.591700378919622	22.170458913763753
260-264	39.16426143679178	22.35170461132909	36.158080046863844	2.3259539050152855
265-269	21.47356537525649	56.85072884963043	18.652600746012492	3.0231050291005896
270-274	17.60620858944375	2.931749148332746	30.04322030148944	49.418821960734064
275-279	40.155621838931395	3.9350965389397885	20.270202232105394	35.63907939002342
280-284	3.3283287865194597	39.332076093523995	12.726959249562103	44.61263587039445
285-289	52.01128553815354	39.63045813547529	5.282809848753037	3.075446477618135
290-294	28.241064316169535	20.00033572716979	23.04858582559889	28.710014131061783
295-299	32.46358190602208	26.605868905594303	21.52167146823833	19.40887772014529
300-301	5.285622933004838	89.08861941396397	5.4794251840925225	0.14633246893867766
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.25
7	0.25
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.25
23	0.0
24	0.25
25	0.25
26	1.0
27	1.75
28	0.25
29	1.5
30	2.5
31	0.75
32	1.0
33	1.5
34	2.583333333333333
35	3.583333333333333
36	2.75
37	8.5
38	10.833333333333336
39	10.333333333333334
40	23.08333333333332
41	14.916666666666673
42	6.916666666666666
43	14.083333333333334
44	23.999999999999996
45	348.58333333333115
46	824.5000000000217
47	259.083333333335
48	167.16666666666674
49	288.24999999999994
50	7658.083333333312
51	6796.833333333303
52	11005.50000000001
53	2800.0000000000023
54	4518.666666666667
55	8606.583333333328
56	876.333333333334
57	142.83333333333331
58	105.74999999999997
59	81.50000000000001
60	52.0
61	36.91666666666667
62	19.41666666666667
63	11.0
64	13.416666666666671
65	14.000000000000004
66	12.583333333333334
67	19.916666666666668
68	17.75
69	24.416666666666664
70	38.91666666666667
71	28.75
72	13.0
73	5.0
74	1.0
75	1.5
76	0.5
77	0.0
78	0.5
79	0.0
80	0.0
81	0.0
82	0.0
83	0.25
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0022708864783849455
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	1.5210675460465174E-4
185-189	1.5216855406396558E-4
190-194	0.0
195-199	3.046597712005118E-4
200-204	0.6313656481629606
205-209	1.5239813708517227E-4
210-214	0.0
215-219	1.5247877114308762E-4
220-224	0.0
225-229	3.0500590948949637E-4
230-234	1.5250946702516558E-4
235-239	0.0
240-244	0.0
245-249	3.050691820637625E-4
250-254	0.0
255-259	0.0
260-264	0.0
265-269	1.5255855960310364E-4
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	3.05205586483055E-4
295-299	4.599674956303088E-4
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-39	2.0
40-49	16.0
50-59	0.0
60-69	21.0
70-79	32.0
80-89	48.0
90-99	3.0
100-109	25.0
110-119	60.0
120-129	25.0
130-139	53.0
140-149	147.0
150-159	19.0
160-169	41.0
170-179	84.0
180-189	120.0
190-199	138.0
200-209	45.0
210-219	79.0
220-229	6.0
230-239	20.0
240-249	7.0
250-259	9.0
260-269	11.0
270-279	14.0
280-289	17.0
290-299	3190.0
300-302	127875.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.447803674294322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.01408781404085	3.869590559167947
2	13.254285043437427	1.7092205560644023
3	6.5977929091336	1.2762382008523394
4	4.355482507630899	1.1233318446410865
5	2.841042498239023	0.9159242129485947
6	2.2540502465367456	0.8720204076998191
7	1.6670579948344681	0.7524203865048786
8	1.3735618689833295	0.708516581256103
9	0.9626672927917351	0.5586380736826966
>10	5.975581122329185	6.075378291839191
>50	0.046959380136182206	0.16123293996533114
>100	0.2817562808170932	5.293436381115308
>500	0.0	0.0
>1k	0.3521953510213665	67.78747530410955
>5k	0.023479690068091103	8.896576260152756
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6105	4.621253983513364	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5648	4.275322276639391	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	5000	3.7848107973082428	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4974	3.7651297811622393	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4952	3.7484766136540837	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4791	3.626605705980758	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4564	3.4547752957829636	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4526	3.426010733723421	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4247	3.214818291233621	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4153	3.143663848244226	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4100	3.103544853792759	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3947	2.987729643395127	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3893	2.9468536867841975	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3794	2.8719144329974946	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3404	2.5766991908074512	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3246	2.457099169612511	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2897	2.1929193759603955	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2671	2.021845927922063	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2420	1.8318484258971894	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2298	1.7394990424428682	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2252	1.7046787831076322	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2237	1.6933243507157076	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1962	1.4851597568637545	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1785	1.3511774546390427	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1777	1.3451217573633494	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1624	1.2293065469657172	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1461	1.1059217149734684	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1417	1.0726153799571558	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1414	1.070344493478771	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1320	0.999190050489376	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1233	0.9333343426162125	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1193	0.9030558562377466	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	416	0.3148962583360458	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	378	0.28613169627650314	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	352	0.2664506801305003	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	347	0.2626658693331921	No Hit
CCTACGGGGGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	346	0.2619089071737304	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	346	0.2619089071737304	No Hit
CCTACGGGGGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	343	0.2596380206953455	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	339	0.25661017205749886	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	332	0.2513114369412673	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	298	0.22557472351957125	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	276	0.20892155601141502	No Hit
CCTACGGGAGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	273	0.20665066953303005	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	271	0.20513674521410674	No Hit
CCTACGGGTGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	265	0.20059497225733686	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	263	0.19908104793841358	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	259	0.19605319930056697	No Hit
CCTACGGGAGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	252	0.19075446418433542	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	247	0.1869696533870272	No Hit
CCTACGGGTGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	245	0.1854557290681039	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	244	0.18469876690864223	No Hit
CCTACGGGCGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	240	0.18167091827079565	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	237	0.1794000317924107	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	218	0.16501775076263936	No Hit
CCTACGGGCGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	206	0.1559342048490996	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	4.5417729567698913E-4	0.0
80-84	0.0	0.0	0.0	7.569621594616486E-4	0.0
85-89	0.0	0.0	0.0	9.083545913539783E-4	0.0
90-94	0.0	0.0	0.0	0.001513924318923297	0.0
95-99	0.0	0.0	0.0	0.001513924318923297	0.0
100-104	0.0	0.0	0.0	0.001513924318923297	0.0
105-109	0.0	0.0	0.0	0.001513924318923297	0.0
110-114	0.0	0.0	0.0	0.0019681016146002863	0.0
115-119	0.0	0.0	0.0	0.0022708864783849455	0.0
120-124	0.0	0.0	0.0	0.0022708864783849455	0.0
125-129	0.0	0.0	0.0	0.0022708864783849455	0.0
130-134	0.0	0.0	0.0	0.0022708864783849455	0.0
135-139	0.0	0.0	0.0	0.0022708864783849455	0.0
140-144	0.0	0.0	0.0	0.0022708864783849455	0.0
145-149	0.0	0.0	0.0	0.0022708864783849455	0.0
150-154	0.0	0.0	0.0	0.0022708864783849455	0.0
155-159	0.0	9.083545913539783E-4	0.0	0.0022708864783849455	0.0
160-164	0.0	0.0022708864783849455	0.0	0.0022708864783849455	0.0
165-169	0.0	0.0022708864783849455	0.0	0.0022708864783849455	0.0
170-174	0.0	0.0022708864783849455	0.0	0.002573671342169605	0.0
175-179	0.0	0.0022708864783849455	0.0	0.003027848637846594	0.0
180-184	0.0	0.0022708864783849455	0.0	0.0033306335016312534	0.0
185-189	0.0	0.0022708864783849455	0.0	0.0037848107973082424	0.0
190-194	0.0	0.0022708864783849455	0.0	0.0037848107973082424	0.0
195-199	0.0	0.0022708864783849455	0.0	0.0037848107973082424	0.0
200-204	0.0	0.0022708864783849455	0.0	0.0037848107973082424	0.0
205-209	0.0	0.0022708864783849455	0.0	0.0037848107973082424	0.0
210-214	0.0	0.0022708864783849455	0.0	0.0037848107973082424	0.0
215-219	0.0	0.0022708864783849455	0.0	0.0037848107973082424	0.0
220-224	0.0	0.0022708864783849455	0.0	0.0042389880929852315	0.0
225-229	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
230-234	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
235-239	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
240-244	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
245-249	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
250-254	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
255-259	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
260-264	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
265-269	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
270-274	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
275-279	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
280-284	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
285-289	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
290	0.0	0.0022708864783849455	0.0	0.004541772956769891	0.0
>>END_MODULE
