FastQCFastQC Report
Mon 14 Oct 2024
DCFS2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDCFS2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71285
Sequences flagged as poor quality0
Sequence length42-301
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG64319.021533281896613No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG63978.973837413200533No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG62978.83355544644736No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG56037.859998597180333No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG53677.528933155642842No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG53507.505085221294802No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG52447.356386336536438No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG51107.168408501087185No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG47736.695658273128989No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA3090.43347127726730733No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA3050.4278599985971803No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2750.38577540857122816No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2650.37174721189591076No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2620.36753875289331556No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2480.3478992775478712No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2340.3282598022024269No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2250.31563442519464124No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA2210.3100231465245143No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG880.12344813074279301No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG860.12064249140772954No Hit
GACTACTAGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG860.12064249140772954No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG840.11783685207266606No Hit
GACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAG840.11783685207266606No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG830.11643403240513434No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG800.1122255734025391No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG800.1122255734025391No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA770.1080171143999439No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA750.1052114750648804No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA740.10380865539734868No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA740.10380865539734868No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG730.10240583572981693No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG730.10240583572981693No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG730.10240583572981693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)