##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DCFS2_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71285
Sequences flagged as poor quality	0
Sequence length	42-301
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21792803535106	34.0	34.0	34.0	20.0	34.0
2	33.0892894718384	34.0	34.0	34.0	34.0	34.0
3	33.56460685978817	34.0	34.0	34.0	34.0	34.0
4	33.43124079399593	34.0	34.0	34.0	34.0	34.0
5	33.22621869958617	34.0	34.0	34.0	34.0	34.0
6	33.04371186084029	34.0	34.0	34.0	34.0	34.0
7	33.489079048888264	34.0	34.0	34.0	34.0	34.0
8	33.46336536438241	34.0	34.0	34.0	34.0	34.0
9	33.38211404923897	34.0	34.0	34.0	34.0	34.0
10-14	33.316728624535315	34.0	34.0	34.0	34.0	34.0
15-19	33.446531528372034	34.0	34.0	34.0	34.0	34.0
20-24	33.67405765588833	34.0	34.0	34.0	34.0	34.0
25-29	33.62130602511047	34.0	34.0	34.0	34.0	34.0
30-34	33.52965701059129	34.0	34.0	34.0	34.0	34.0
35-39	33.56783895630217	34.0	34.0	34.0	34.0	34.0
40-44	33.34668898232905	34.0	34.0	34.0	34.0	34.0
45-49	33.58548321479175	34.0	34.0	34.0	34.0	34.0
50-54	33.54063017311523	34.0	34.0	34.0	34.0	34.0
55-59	33.33990229099383	34.0	34.0	34.0	34.0	34.0
60-64	33.291959377434374	34.0	34.0	34.0	31.2	34.0
65-69	33.51113106680324	34.0	34.0	34.0	34.0	34.0
70-74	33.55169030015254	34.0	34.0	34.0	34.0	34.0
75-79	33.36199202315941	34.0	34.0	34.0	34.0	34.0
80-84	33.58414190357677	34.0	34.0	34.0	34.0	34.0
85-89	33.54048631531439	34.0	34.0	34.0	34.0	34.0
90-94	33.03936435890172	34.0	34.0	34.0	34.0	34.0
95-99	33.282904637270235	34.0	34.0	34.0	34.0	34.0
100-104	33.265046576454516	34.0	34.0	34.0	34.0	34.0
105-109	33.34187370396037	34.0	34.0	34.0	34.0	34.0
110-114	33.198508553577945	34.0	34.0	34.0	34.0	34.0
115-119	33.0557198805581	34.0	34.0	34.0	34.0	34.0
120-124	33.2173082750768	34.0	34.0	34.0	34.0	34.0
125-129	33.278037941031464	34.0	34.0	34.0	34.0	34.0
130-134	32.99581033247411	34.0	34.0	34.0	34.0	34.0
135-139	33.099023894468985	34.0	34.0	34.0	34.0	34.0
140-144	33.21539452952827	34.0	34.0	34.0	34.0	34.0
145-149	33.315047512069	34.0	34.0	34.0	34.0	34.0
150-154	32.970665261074586	34.0	34.0	34.0	34.0	34.0
155-159	33.1090019382497	34.0	34.0	34.0	34.0	34.0
160-164	33.2171448861443	34.0	34.0	34.0	34.0	34.0
165-169	33.24746306942102	34.0	34.0	34.0	34.0	34.0
170-174	32.83823240813668	34.0	34.0	34.0	31.2	34.0
175-179	33.19201970391081	34.0	34.0	34.0	34.0	34.0
180-184	32.94268096111443	34.0	34.0	34.0	34.0	34.0
185-189	33.13983402085506	34.0	34.0	34.0	34.0	34.0
190-194	33.072608783504485	34.0	34.0	34.0	34.0	34.0
195-199	32.518833767968474	34.0	34.0	34.0	28.4	34.0
200-204	32.898205883213635	34.0	34.0	34.0	34.0	34.0
205-209	32.62524063902331	34.0	34.0	34.0	31.2	34.0
210-214	32.659880989252365	34.0	34.0	34.0	31.2	34.0
215-219	32.86975946459571	34.0	34.0	34.0	34.0	34.0
220-224	32.8556493150895	34.0	34.0	34.0	34.0	34.0
225-229	32.98952883106857	34.0	34.0	34.0	34.0	34.0
230-234	32.69611142765832	34.0	34.0	34.0	31.2	34.0
235-239	32.748260362702375	34.0	34.0	34.0	34.0	34.0
240-244	32.53530539024841	34.0	34.0	34.0	31.2	34.0
245-249	32.794924892974464	34.0	34.0	34.0	34.0	34.0
250-254	32.27972291920664	34.0	34.0	34.0	31.2	34.0
255-259	32.263786704371356	34.0	34.0	34.0	28.4	34.0
260-264	32.51279293519032	34.0	34.0	34.0	31.2	34.0
265-269	32.67653394475966	34.0	34.0	34.0	34.0	34.0
270-274	32.32913286611533	34.0	34.0	34.0	31.2	34.0
275-279	32.44499884832012	34.0	34.0	34.0	31.2	34.0
280-284	31.922337945351767	34.0	34.0	34.0	22.8	34.0
285-289	31.513470988631525	34.0	34.0	34.0	20.0	34.0
290-294	31.718839715712612	34.0	34.0	34.0	20.0	34.0
295-299	31.769205963620664	34.0	34.0	34.0	20.0	34.0
300-301	31.56817737751156	34.0	34.0	34.0	20.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.043470422704295686
1101	2	-0.0180511834825694
1101	3	0.12051210882174246
1101	4	0.11382545088414986
1101	5	-0.13402172828871528
1101	6	-0.23401649032150118
1101	7	0.2574228990127452
1101	8	-0.012416349668100679
1101	9	-0.2762927589312696
1101	10-14	-0.08033247750188366
1101	15-19	-0.05436030492666788
1101	20-24	-0.15739466474187225
1101	25-29	0.013205445229246493
1101	30-34	-0.10789635935431363
1101	35-39	-0.1843018855974492
1101	40-44	-0.3593508088124011
1101	45-49	-0.3017276249406322
1101	50-54	-0.13143557381699367
1101	55-59	-0.17910882020416352
1101	60-64	-0.22518417548793934
1101	65-69	-0.08390670387123578
1101	70-74	-0.41872318776739803
1101	75-79	-0.36295766723113587
1101	80-84	-0.10534290421986725
1101	85-89	-0.14001950848876987
1101	90-94	-0.3723420555439887
1101	95-99	-0.07430667752856834
1101	100-104	-0.3031358982432977
1101	105-109	-0.0664336916171564
1101	110-114	-0.2488478420189537
1101	115-119	-0.39655920910406195
1101	120-124	-0.35320431445952494
1101	125-129	-0.23617640319742605
1101	130-134	-0.31319778867845116
1101	135-139	-0.36815536059965837
1101	140-144	-0.17764795268834632
1101	145-149	-0.1838130590164937
1101	150-154	-0.05154226447388055
1101	155-159	-0.33472749469665786
1101	160-164	-0.23352053434469866
1101	165-169	-0.11724260291521915
1101	170-174	-0.3586951498334514
1101	175-179	-0.3351089457402736
1101	180-184	-0.6585619458600362
1101	185-189	-0.4833903541474882
1101	190-194	-0.5297896060906666
1101	195-199	-0.33407436705666527
1101	200-204	-0.23712954053424795
1101	205-209	-0.11784482715571443
1101	210-214	-0.325502654775363
1101	215-219	-0.48280554215043736
1101	220-224	-0.6602171774932657
1101	225-229	-0.4458570634891643
1101	230-234	-0.44403158917846497
1101	235-239	-0.4184377727007913
1101	240-244	-0.5406883156239708
1101	245-249	-0.5429593333460474
1101	250-254	-0.4470957918311882
1101	255-259	-0.6106143912885216
1101	260-264	-0.5318965267810434
1101	265-269	-0.38460558810424317
1101	270-274	-0.5879679320543687
1101	275-279	-0.8445186616943694
1101	280-284	-0.7253978328133819
1101	285-289	-0.7965156848083694
1101	290-294	-0.6695943532211466
1101	295-299	-0.89420523866632
1101	300-301	-0.7637688322115181
1102	1	0.3812585830056321
1102	2	-0.10396052956468083
1102	3	0.12536533494947832
1102	4	0.12251779618756586
1102	5	-0.38849013704624724
1102	6	0.1365490553948021
1102	7	0.2614793268210107
1102	8	-0.23943143450901516
1102	9	0.08083586967016743
1102	10-14	0.02007497618420473
1102	15-19	-0.0770871660845529
1102	20-24	-0.08590374643180354
1102	25-29	0.0035171020264783692
1102	30-34	-0.027318318391628793
1102	35-39	0.15405127714861067
1102	40-44	0.1131614600708204
1102	45-49	0.06063814170612147
1102	50-54	0.01566063958008357
1102	55-59	0.09177363393465754
1102	60-64	0.0771211069227391
1102	65-69	0.12334053902548447
1102	70-74	0.03404297003488921
1102	75-79	0.03376821603790603
1102	80-84	0.016473071586439403
1102	85-89	0.044776130851339246
1102	90-94	-0.08908387477942625
1102	95-99	-0.22700583183581102
1102	100-104	-0.2138799992193725
1102	105-109	-0.15090517891365351
1102	110-114	-0.052563819636162634
1102	115-119	-0.16619508516325254
1102	120-124	-0.22200114285844563
1102	125-129	-0.027712738397823955
1102	130-134	-0.451846221063704
1102	135-139	-0.3395091105087289
1102	140-144	0.03574769727165261
1102	145-149	-0.05459213808828878
1102	150-154	-0.05795457261383774
1102	155-159	-0.1961081595690004
1102	160-164	-0.393691656319902
1102	165-169	-0.21841150998711356
1102	170-174	-0.48175184182643704
1102	175-179	-0.17105283820082207
1102	180-184	-0.4420657448080192
1102	185-189	-0.17294324719249232
1102	190-194	-0.4594798457166078
1102	195-199	-0.3403718539064613
1102	200-204	-0.15739546689310657
1102	205-209	-0.22548655713817567
1102	210-214	-0.240713058048307
1102	215-219	-0.2351871903094107
1102	220-224	-0.34480221548275125
1102	225-229	-0.2379084954003332
1102	230-234	-0.622479865040539
1102	235-239	-0.21824782530161713
1102	240-244	-0.22204424782736254
1102	245-249	-0.33464548179724574
1102	250-254	-0.4360788426786506
1102	255-259	-0.5357751160109032
1102	260-264	-0.541890682245679
1102	265-269	-0.267977885006637
1102	270-274	-0.21467745864701016
1102	275-279	-0.08708149044948499
1102	280-284	-0.6830103401190506
1102	285-289	-0.960542569671027
1102	290-294	-0.7259356740861342
1102	295-299	-1.09152451819228
1102	300-301	-0.665597996148076
1103	1	0.3812585830056321
1103	2	0.1618622552454454
1103	3	-0.48644901104208316
1103	4	-0.2192543557111648
1103	5	-0.16486144506312428
1103	6	-0.8592315353224933
1103	7	-0.4558202512380589
1103	8	-0.4039883965343307
1103	9	0.19897932958577513
1103	10-14	-0.2702203824655882
1103	15-19	-0.12181290448118176
1103	20-24	0.006079375931051345
1103	25-29	-0.20239007096930095
1103	30-34	-0.018879499826233825
1103	35-39	0.012279125249868628
1103	40-44	-0.028610691827914536
1103	45-49	-0.08535341947531805
1103	50-54	0.008065702871220992
1103	55-59	0.03270190397685724
1103	60-64	-0.20389155130510517
1103	65-69	-0.07665946097451126
1103	70-74	-0.16933255739127162
1103	75-79	0.011827287767864902
1103	80-84	-0.12698684402536742
1103	85-89	-0.17885256113178372
1103	90-94	-0.18866193385115793
1103	95-99	-0.2607611060974193
1103	100-104	-0.034133163776338904
1103	105-109	-0.17453387089677364
1103	110-114	-0.16311234284292198
1103	115-119	-0.1879429218220494
1103	120-124	0.0525000371458475
1103	125-129	-0.11129637567166384
1103	130-134	0.15363185946264935
1103	135-139	-0.001570184670214303
1103	140-144	-0.14579775077240953
1103	145-149	-0.06653521182067124
1103	150-154	-0.1677915174754503
1103	155-159	-0.09920477689001928
1103	160-164	-0.12005938856619736
1103	165-169	-0.10266381635913291
1103	170-174	-0.13212014619603707
1103	175-179	0.10411701090166048
1103	180-184	-0.09056034539445079
1103	185-189	-0.10857222523726051
1103	190-194	-0.07623948241832323
1103	195-199	-0.3697611122916342
1103	200-204	0.15621034015831015
1103	205-209	-0.17229339401152544
1103	210-214	-0.23304303015729744
1103	215-219	-0.16340717171540575
1103	220-224	-0.19989053701603865
1103	225-229	-0.2290012508891408
1103	230-234	-0.05813387347934906
1103	235-239	-0.32052201538957803
1103	240-244	-0.417270594104977
1103	245-249	-0.39801171221991183
1103	250-254	-0.543931234869163
1103	255-259	-0.4780085423142708
1103	260-264	-0.4681761717686683
1103	265-269	-0.24597616863675853
1103	270-274	-0.5391464567160895
1103	275-279	-0.4723922249127597
1103	280-284	-0.5186285908520851
1103	285-289	-0.9103888115361514
1103	290-294	-0.8135635078843215
1103	295-299	-0.8054790217886953
1103	300-301	-1.4005344575779013
1104	1	0.39640197140583666
1104	2	-0.36241722698296996
1104	3	0.06484541790735676
1104	4	-0.21013614073584108
1104	5	0.23364030706445504
1104	6	-0.3966519744271224
1104	7	0.09522387377087682
1104	8	0.13439750062650546
1104	9	0.1251039094327382
1104	10-14	-0.03966542287178498
1104	15-19	-0.27550309971825016
1104	20-24	-0.012235720972320507
1104	25-29	0.04425585622801265
1104	30-34	-0.44635929672245567
1104	35-39	-0.15213123018172325
1104	40-44	-0.03672769104124285
1104	45-49	-0.2790135782395282
1104	50-54	-0.07615442156559737
1104	55-59	-0.18525561586866957
1104	60-64	-0.19325720960447512
1104	65-69	-0.33840586732508626
1104	70-74	-0.06944698204979005
1104	75-79	-0.18152535472659537
1104	80-84	-0.31147515125558556
1104	85-89	-0.26121686063832783
1104	90-94	-0.34437959100627324
1104	95-99	-0.5437654685375151
1104	100-104	-0.4172662539572727
1104	105-109	-0.32730151732458523
1104	110-114	-0.49359578702514284
1104	115-119	-0.39536622154314216
1104	120-124	-0.6160689394686187
1104	125-129	-0.35144155195715854
1104	130-134	-0.347608932928118
1104	135-139	-0.4462887715256727
1104	140-144	-0.4176818014661876
1104	145-149	-0.4280793360688122
1104	150-154	-0.6910126324767987
1104	155-159	-0.7270277412494863
1104	160-164	-0.4228441986927791
1104	165-169	-0.2959953541991496
1104	170-174	-0.5858484880579553
1104	175-179	-0.6783517924020543
1104	180-184	-0.3548605518761718
1104	185-189	-0.2873926426419331
1104	190-194	-0.43838069681140013
1104	195-199	-0.7828793100787159
1104	200-204	-0.1907591098697594
1104	205-209	-0.28077586163846746
1104	210-214	-0.3694681720167452
1104	215-219	-0.33194347318492845
1104	220-224	-0.3576309705967162
1104	225-229	-0.22689154624778496
1104	230-234	-0.5147212443508806
1104	235-239	-0.4511963933904468
1104	240-244	-0.7579296949343188
1104	245-249	-0.39123519541501395
1104	250-254	-0.968647515969117
1104	255-259	-1.0192350809436945
1104	260-264	-1.1706896302293153
1104	265-269	-0.8130538639663065
1104	270-274	-0.7052093113647118
1104	275-279	-1.0005531444529865
1104	280-284	-0.967639212123725
1104	285-289	-0.7473777537738897
1104	290-294	-1.4032150428763188
1104	295-299	-1.2254268615888186
1104	300-301	-1.4304723640368202
1201	1	-0.24342853706874124
1201	2	0.15876921369499541
1201	3	0.030947112822609313
1201	4	0.15427063892517623
1201	5	0.17431827316615056
1201	6	-0.006821465952548067
1201	7	0.04861370427935441
1201	8	-0.07746690615315543
1201	9	0.03188357044968626
1201	10-14	0.1188091533993969
1201	15-19	0.10924266299361562
1201	20-24	-0.0495238565655427
1201	25-29	-0.08286278783978673
1201	30-34	-0.034494889942799034
1201	35-39	-0.05975834882578823
1201	40-44	-0.15876158934633366
1201	45-49	-0.11799662908698139
1201	50-54	-0.1303917097011862
1201	55-59	-0.15898442942798852
1201	60-64	-0.05851144689260224
1201	65-69	-0.18755840969796367
1201	70-74	-0.2330063040836876
1201	75-79	0.031128810437493826
1201	80-84	0.04374302400587027
1201	85-89	-0.1127927712045036
1201	90-94	-0.31589058992441466
1201	95-99	-0.20705071916860618
1201	100-104	0.03208462956239089
1201	105-109	0.02246047329929013
1201	110-114	-0.06263653711529571
1201	115-119	-0.05476759190015201
1201	120-124	-0.03415765205426169
1201	125-129	0.07190139791662631
1201	130-134	0.12730992751184544
1201	135-139	-0.0403241123118292
1201	140-144	0.1584126810437354
1201	145-149	0.19660151499501666
1201	150-154	-0.08420412183850345
1201	155-159	0.019355237473916986
1201	160-164	-0.22880164550129223
1201	165-169	-0.1513145031353318
1201	170-174	-0.3722314667813649
1201	175-179	-0.25792626048715306
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2404	285-289	0.7699348055427819
2404	290-294	0.6539741377627202
2404	295-299	0.63403012209994
2404	300-301	1.0662471162622644
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	10.0
16	20.0
17	31.0
18	64.0
19	115.0
20	183.0
21	231.0
22	236.0
23	272.0
24	283.0
25	289.0
26	371.0
27	407.0
28	586.0
29	817.0
30	1220.0
31	2204.0
32	6841.0
33	55282.0
34	1823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.46573612962053734	0.07014098337658695	0.11362839307007085	99.3504944939328
2	0.07715724646830241	0.15992592904339042	0.39420338650169046	99.36871343798663
3	0.2805639335063478	0.19779757312197518	99.32384092024971	0.19779757312197518
4	0.09539173739215824	99.35189731360033	0.34228799887774425	0.2104229501297608
5	0.17254681910640388	0.34088517921021255	0.21463140913235607	99.27193659255103
6	99.19618433050431	0.32405134319983164	0.1122255734025391	0.36753875289331556
7	99.71803324682612	0.061724065371396505	0.06452970470645998	0.155712983096023
8	99.37293960861331	0.20060321245703866	0.12765658974538824	0.2988005891842604
9	30.017535245844147	23.05534123588413	25.525706670407516	21.40141684786421
10-14	59.64873395525005	10.130041383180192	9.95581118047275	20.265413481097006
15-19	22.184470786280425	37.93112155432419	19.610296696359683	20.274110963035703
20-24	56.54934418180543	40.13579294381707	2.645156765097847	0.6697061092796521
25-29	1.7768113908957002	2.850249000490987	58.18194571087887	37.19099389773445
30-34	3.008487058988567	57.944588623132496	0.8947183839517431	38.15220593392719
35-39	43.643964368380445	16.976081924668584	20.65904467980641	18.72090902714456
40-44	38.13018873747619	46.84656627976466	1.7973127284868176	13.225932254272335
45-49	20.37119649846387	20.038718909136822	35.351205757333446	24.238878835065865
50-54	55.996464745658095	20.376252069246092	19.936870458180188	3.690412726915631
55-59	17.198547731812976	41.42920473165586	2.4702026890529956	38.90204484747817
60-64	38.97619234697886	2.198924670834783	1.1985205805430525	57.626362401643306
65-69	40.27164685908319	20.79445481205012	36.35605943678177	2.577838892084918
70-74	40.90155216492068	55.36915482641626	3.1371635596240677	0.5921294490389823
75-79	45.88080778599733	21.618883513128207	29.61311361314729	2.887195087727176
80-84	38.785051974585215	1.407411149278193	39.671033429873596	20.136503446262992
85-89	21.778351600544507	11.775123847481652	38.630871352989885	27.81565319898396
90-94	18.973014398122544	56.23661317081524	2.2979729217605627	22.492399509301652
95-99	20.865710692388888	1.0322388663058368	57.80593797022031	20.29611247108496
100-104	36.41263763103373	9.459133198205533	48.71422716068792	5.414002010072823
105-109	39.803072119852544	44.113922806741705	15.092188864337636	0.9908162090681162
110-114	46.10646413464185	46.33032418980636	1.1622746653334306	6.400937010218355
115-119	23.443448843632602	48.87313535581724	22.744936063321337	4.938479737228821
120-124	54.65234239981116	24.103773187243046	18.089773024254395	3.1541113886913976
125-129	3.512213768350165	37.68237737747585	36.85266746677085	21.952741387403137
130-134	36.707152496626186	7.39231893837157	22.507309941520468	33.39321862348178
135-139	8.574056209764288	23.481484189773344	59.04415838632471	8.900301214137658
140-144	40.591792303770944	18.718121315732404	19.668672917306235	21.021413463190417
145-149	17.836099941671574	37.043622336123896	37.74694480214829	7.373332920056243
150-154	4.4980046445561745	31.071742610420944	21.38778662097265	43.042466124050236
155-159	21.59670995329329	71.99824109908756	1.614017087271364	4.791031860347777
160-164	21.699533640480233	19.558903970540968	17.600137595795434	41.14142479318337
165-169	37.85575697413394	22.81103001725908	20.601755236945706	18.73145777166127
170-174	2.4757041905863435	40.23442458778227	20.73938248444927	36.55048873718211
175-179	40.59206163310804	4.116775549942854	19.20051925329119	36.09064356365791
180-184	37.40423754170642	39.244683313479854	1.154795038700171	22.196284106113552
185-189	39.6470322471339	1.6044502174281359	5.435421019935618	53.313096515502345
190-194	19.625832746842136	39.26805898264269	37.13266572262782	3.97344254788736
195-199	20.89399852932858	37.947564907517396	21.256292776740764	19.90214378641326
200-204	73.25176068862488	3.2706836451589956	21.131962723198406	2.3455929430177136
205-209	21.249027512553926	38.31756135511705	3.0816889454699767	37.35172218685904
210-214	35.834022124344706	7.847122363251395	52.48389361292587	3.834961899478029
215-219	37.94579778513361	20.194303415741917	3.5771423070061923	38.28275649211828
220-224	4.787054788895528	21.72582047411779	71.247086676163	2.240038060823688
225-229	55.1536537911464	2.0940302861237217	21.170549902435294	21.58176602029459
230-234	20.456090458979617	55.80069339799005	21.13022739375262	2.6129887492777106
235-239	54.7835077829554	20.749259946461198	3.150857612282764	21.31637465830064
240-244	38.39531319495398	21.5220784665898	9.074169029805294	31.00843930865093
245-249	53.32145245191926	13.889014812380292	12.532157711617575	20.25737502408287
250-254	16.085853934972018	41.34419494226819	30.440178508181624	12.129772614578169
255-259	21.716486213081634	36.48751654118507	19.511939406132495	22.284057839600802
260-264	38.3416693823767	20.639585133967156	37.052297490683934	3.9664479929722147
265-269	22.55077836063901	56.11423324462884	17.911505227330053	3.4234831674020922
270-274	19.70342826047524	4.098834572827862	28.310357337202973	47.88737982949392
275-279	39.01594176606649	4.754750782348406	19.96264002902626	36.26666742255885
280-284	3.4339156220056357	38.748561287725444	15.37678669637643	42.44073639389249
285-289	50.78548135545158	41.11271799234368	4.017297603856515	4.08450304834822
290-294	27.73776871388902	19.794417388295294	23.799988087100317	28.66782581071537
295-299	33.922587371327424	26.271545674825713	19.909377846263123	19.896489107583733
300-301	5.220363832206331	93.62545628627849	0.9380049069475196	0.21617497456765006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.3333333333333333
23	0.5833333333333333
24	0.0
25	1.0
26	1.0833333333333333
27	1.1666666666666665
28	0.0
29	1.3333333333333333
30	0.8333333333333333
31	1.25
32	0.25
33	0.25
34	0.75
35	1.0
36	1.2499999999999998
37	5.499999999999999
38	5.833333333333333
39	3.9166666666666665
40	14.583333333333341
41	11.500000000000002
42	5.166666666666667
43	11.833333333333334
44	32.91666666666664
45	395.41666666666185
46	918.5000000000324
47	257.1666666666685
48	106.49999999999996
49	180.75
50	3958.1666666666742
51	3788.0000000000146
52	7718.99999999998
53	1842.0833333333305
54	1316.3333333333335
55	2793.083333333335
56	597.333333333333
57	121.24999999999994
58	51.83333333333334
59	43.75000000000001
60	27.58333333333333
61	17.16666666666667
62	10.083333333333332
63	10.833333333333334
64	12.083333333333336
65	11.166666666666668
66	6.249999999999998
67	11.666666666666668
68	14.583333333333332
69	10.583333333333334
70	15.0
71	14.25
72	5.0
73	2.25
74	0.25
75	0.0
76	0.0
77	0.0
78	0.0
79	0.5
80	0.5
81	0.0
82	0.0
83	0.25
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002805639335063478
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.6021710969187167
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40-49	3.0
50-59	2.0
60-69	11.0
70-79	3.0
80-89	19.0
90-99	5.0
100-109	19.0
110-119	49.0
120-129	28.0
130-139	50.0
140-149	129.0
150-159	22.0
160-169	29.0
170-179	49.0
180-189	48.0
190-199	107.0
200-209	23.0
210-219	62.0
220-229	16.0
230-239	10.0
240-249	14.0
250-259	9.0
260-269	8.0
270-279	18.0
280-289	30.0
290-299	3394.0
300-302	67128.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.238759907413902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.693682955899874	5.165182015851863
2	13.774902094329985	2.2697622220663534
3	6.981099948918781	1.7254681910640386
4	4.324876553720416	1.4252647822122466
5	2.707304614336796	1.1152416356877324
6	1.7878426698450536	0.8837763905449955
7	1.2259492593223225	0.7070211124359963
8	1.0556785288608888	0.6957985550957424
9	0.8172995062148816	0.6060180963737112
>10	3.677847777966967	4.76818404994038
>50	0.1532436574152903	1.0310724556358282
>100	0.39162268006129747	6.569404503051132
>500	0.06810829218457347	5.038928245774006
>1k	0.34054146092286736	67.99887774426597
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4298	6.029318931051413	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3802	5.333520375955671	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3570	5.008066213088307	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3408	4.780809426948166	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3376	4.73591919758715	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3183	4.465175001753525	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3090	4.334712772673073	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	2680	3.7595567089850603	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2650	3.717472118959108	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2333	3.2727782843515465	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2211	3.1016342849126746	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	2067	2.899628252788104	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1972	2.766360384372589	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1894	2.6569404503051133	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1771	2.484393631198709	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	1671	2.3441116644455353	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1281	1.7970119941081573	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1131	1.5865890439783967	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1070	1.5010170442589605	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1015	1.4238619625447149	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	996	1.3972083888616118	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	930	1.304622290804517	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	868	1.2176474714175494	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	798	1.1194500946903276	No Hit
CCTACGGGGGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	356	0.499403801641299	No Hit
CCTACGGGTGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	346	0.4853756049659816	No Hit
CCTACGGGGGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	341	0.47836150662832294	No Hit
CCTACGGGTGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	316	0.4432910149400294	No Hit
CCTACGGGAGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	308	0.4320684575997756	No Hit
CCTACGGGCGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	285	0.39980360524654557	No Hit
CCTACGGGAGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	282	0.39559514624395037	No Hit
CCTACGGGCGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	246	0.34509363821280775	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	182	0.2553131794907765	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	181	0.2539103598232447	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	176	0.24689626148558602	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	167	0.2342708844778004	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	165	0.2314652451427369	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	162	0.2272567861401417	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	160	0.2244511468050782	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	150	0.2104229501297608	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	133	0.18657501578172125	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	130	0.18236655677912603	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	127	0.17815809777653083	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	126	0.17675527810899908	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	125	0.17535245844146735	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	117	0.16412990110121345	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	102	0.14308760608823737	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	94	0.13186504874798344	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	94	0.13186504874798344	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	88	0.12344813074279301	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	88	0.12344813074279301	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	85	0.1192396717401978	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	77	0.1080171143999439	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	77	0.1080171143999439	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	5.611278670126956E-4	0.0	0.0	0.0
160-164	0.0	0.001402819667531739	0.0	0.0	0.0
165-169	0.0	0.001402819667531739	0.0	0.0	0.0
170-174	0.0	0.001402819667531739	0.0	0.0	0.0
175-179	0.0	0.001402819667531739	0.0	0.0	0.0
180-184	0.0	0.001402819667531739	0.0	0.0	0.0
185-189	0.0	0.001402819667531739	0.0	0.0	0.0
190-194	0.0	0.001402819667531739	0.0	0.0	0.0
195-199	0.0	0.001402819667531739	0.0	0.0	0.0
200-204	0.0	0.001402819667531739	0.0	0.0	0.0
205-209	0.0	0.001402819667531739	0.0	0.0	0.0
210-214	0.0	0.001402819667531739	0.0	0.0	0.0
215-219	0.0	0.001402819667531739	0.0	0.0	0.0
220-224	0.0	0.001402819667531739	0.0	0.0	0.0
225-229	0.0	0.001402819667531739	0.0	0.0	0.0
230-234	0.0	0.001402819667531739	0.0	0.0	0.0
235-239	0.0	0.001402819667531739	0.0	0.0	0.0
240-244	0.0	0.001402819667531739	0.0	0.0	0.0
245-249	0.0	0.001402819667531739	0.0	0.0	0.0
250-254	0.0	0.001402819667531739	0.0	0.0	0.0
255-259	0.0	0.001402819667531739	0.0	0.0	0.0
260-264	0.0	0.001402819667531739	0.0	0.0	0.0
265-269	0.0	0.001402819667531739	0.0	0.0	0.0
270-274	0.0	0.001402819667531739	0.0	0.0	0.0
275-279	0.0	0.001402819667531739	0.0	0.0	0.0
280-284	0.0	0.001402819667531739	0.0	0.0	0.0
285-289	0.0	0.001402819667531739	0.0	0.0	0.0
290	0.0	0.001402819667531739	0.0	0.0	0.0
>>END_MODULE
