##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DCFS1_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	122736
Sequences flagged as poor quality	0
Sequence length	35-301
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.253275322643724	34.0	34.0	34.0	20.0	34.0
2	33.08409105722852	34.0	34.0	34.0	34.0	34.0
3	33.57861589101812	34.0	34.0	34.0	34.0	34.0
4	33.43613935601616	34.0	34.0	34.0	34.0	34.0
5	33.20550612697171	34.0	34.0	34.0	34.0	34.0
6	33.081100899491595	34.0	34.0	34.0	34.0	34.0
7	33.526235171424844	34.0	34.0	34.0	34.0	34.0
8	33.4587895971842	34.0	34.0	34.0	34.0	34.0
9	33.37884565245731	34.0	34.0	34.0	34.0	34.0
10-14	33.30504171555208	34.0	34.0	34.0	34.0	34.0
15-19	33.43514372311302	34.0	34.0	34.0	34.0	34.0
20-24	33.705292660670054	34.0	34.0	34.0	34.0	34.0
25-29	33.61881436579324	34.0	34.0	34.0	34.0	34.0
30-34	33.54735366966497	34.0	34.0	34.0	34.0	34.0
35-39	33.58114912319723	34.0	34.0	34.0	34.0	34.0
40-44	33.40020091104883	34.0	34.0	34.0	34.0	34.0
45-49	33.59607756864663	34.0	34.0	34.0	34.0	34.0
50-54	33.54410005703577	34.0	34.0	34.0	34.0	34.0
55-59	33.358851136641405	34.0	34.0	34.0	34.0	34.0
60-64	33.3136262036178	34.0	34.0	34.0	34.0	34.0
65-69	33.52280149934811	34.0	34.0	34.0	34.0	34.0
70-74	33.55627933507171	34.0	34.0	34.0	34.0	34.0
75-79	33.351525423728816	34.0	34.0	34.0	34.0	34.0
80-84	33.597628748370276	34.0	34.0	34.0	34.0	34.0
85-89	33.543685412716364	34.0	34.0	34.0	34.0	34.0
90-94	33.084128691570506	34.0	34.0	34.0	34.0	34.0
95-99	33.2938363901683	34.0	34.0	34.0	34.0	34.0
100-104	33.26897603157433	34.0	34.0	34.0	34.0	34.0
105-109	33.33632461120285	34.0	34.0	34.0	34.0	34.0
110-114	33.20806947591759	34.0	34.0	34.0	34.0	34.0
115-119	33.01300293446364	34.0	34.0	34.0	31.2	34.0
120-124	33.18251411480217	34.0	34.0	34.0	34.0	34.0
125-129	33.24598115704496	34.0	34.0	34.0	34.0	34.0
130-134	32.95612892663383	34.0	34.0	34.0	34.0	34.0
135-139	33.13443178388608	34.0	34.0	34.0	34.0	34.0
140-144	33.192854630972974	34.0	34.0	34.0	34.0	34.0
145-149	33.29038443886164	34.0	34.0	34.0	34.0	34.0
150-154	32.975342666927745	34.0	34.0	34.0	34.0	34.0
155-159	33.08657853140302	34.0	34.0	34.0	34.0	34.0
160-164	33.19095564809383	34.0	34.0	34.0	34.0	34.0
165-169	33.24561544445932	34.0	34.0	34.0	34.0	34.0
170-174	32.8346619835677	34.0	34.0	34.0	31.2	34.0
175-179	33.19733980817332	34.0	34.0	34.0	34.0	34.0
180-184	32.87155681666068	34.0	34.0	34.0	34.0	34.0
185-189	33.115271122832866	34.0	34.0	34.0	34.0	34.0
190-194	33.07363600703881	34.0	34.0	34.0	34.0	34.0
195-199	32.50577722558252	34.0	34.0	34.0	28.4	34.0
200-204	32.91284877891892	34.0	34.0	34.0	34.0	34.0
205-209	32.63165959215861	34.0	34.0	34.0	31.2	34.0
210-214	32.673100585273616	34.0	34.0	34.0	31.2	34.0
215-219	32.88575376205287	34.0	34.0	34.0	34.0	34.0
220-224	32.8827460642261	34.0	34.0	34.0	34.0	34.0
225-229	33.0211597081898	34.0	34.0	34.0	34.0	34.0
230-234	32.73026549977031	34.0	34.0	34.0	31.2	34.0
235-239	32.81412008693395	34.0	34.0	34.0	34.0	34.0
240-244	32.59401440461613	34.0	34.0	34.0	31.2	34.0
245-249	32.820859651096136	34.0	34.0	34.0	34.0	34.0
250-254	32.361775748334395	34.0	34.0	34.0	31.2	34.0
255-259	32.2760004502779	34.0	34.0	34.0	28.4	34.0
260-264	32.601715933135736	34.0	34.0	34.0	31.2	34.0
265-269	32.69998551732917	34.0	34.0	34.0	34.0	34.0
270-274	32.44702526467372	34.0	34.0	34.0	34.0	34.0
275-279	32.51144239383457	34.0	34.0	34.0	31.2	34.0
280-284	31.95149515291603	34.0	34.0	34.0	22.8	34.0
285-289	31.70308293179185	34.0	34.0	34.0	20.0	34.0
290-294	31.79897838405836	34.0	34.0	34.0	20.0	34.0
295-299	31.869485192265643	34.0	34.0	34.0	20.0	34.0
300-301	31.68210762670249	34.0	34.0	34.0	20.0	34.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.20069334423927643
1101	2	-0.10041952972495949
1101	3	0.1406611178997963
1101	4	0.2084335890525253
1101	5	0.2747986054971818
1101	6	-0.10090141374399764
1101	7	-0.11681396634806163
1101	8	0.03610715980046564
1101	9	0.09749044241276295
1101	10-14	0.004717754153489295
1101	15-19	-0.03901243109790897
1101	20-24	-0.005694587595115763
1101	25-29	0.0946644081817638
1101	30-34	0.02111506891935022
1101	35-39	0.02360782902277947
1101	40-44	-0.08635315894313322
1101	45-49	-0.04975039619452559
1101	50-54	-0.0718490873466422
1101	55-59	-0.1725938837223353
1101	60-64	-0.13544006664445618
1101	65-69	-0.10391327369809744
1101	70-74	-0.09762995642765304
1101	75-79	0.05422809112638305
1101	80-84	-0.008459825569318014
1101	85-89	-0.10918481833596871
1101	90-94	-0.27002459642104526
1101	95-99	0.009239267945361007
1101	100-104	0.01852783856143958
1101	105-109	-0.1624667665990387
1101	110-114	-0.33168573209044183
1101	115-119	-0.20626562946292637
1101	120-124	-0.03670656383070536
1101	125-129	-0.20565043467735222
1101	130-134	-0.09963662334202894
1101	135-139	-0.07898124831532272
1101	140-144	-0.0478051548535845
1101	145-149	-0.04533446539191743
1101	150-154	-0.14217662089112793
1101	155-159	-0.26698189598591426
1101	160-164	-0.29165957449043844
1101	165-169	-0.03198823272958151
1101	170-174	-0.31276043138832677
1101	175-179	-0.1450364174040928
1101	180-184	-0.23123681270468666
1101	185-189	-0.18066779936648913
1101	190-194	-0.04099551354926234
1101	195-199	-0.17709396651546427
1101	200-204	0.12882027335349022
1101	205-209	-0.13290497792893063
1101	210-214	-0.13564407796962286
1101	215-219	-0.1247987111930513
1101	220-224	-0.039556465318284495
1101	225-229	-0.04617817425135229
1101	230-234	-0.20628690990599097
1101	235-239	-0.34823664958303624
1101	240-244	-0.2459196164214461
1101	245-249	-0.3528573781947273
1101	250-254	-0.3188925427496585
1101	255-259	-0.41466244886434467
1101	260-264	-0.14070930498196077
1101	265-269	-0.5674518321739299
1101	270-274	-0.4050955703096548
1101	275-279	-0.48282810019714617
1101	280-284	-0.8027997095295518
1101	285-289	-0.7528230647641081
1101	290-294	-0.8871308356596934
1101	295-299	-0.7116052443687906
1101	300-301	-0.5952101934900291
1102	1	0.10252815795823977
1102	2	0.30521399084228307
1102	3	0.1555435613317755
1102	4	-0.06022251157047265
1102	5	0.127919203388295
1102	6	0.2893133060240132
1102	7	0.1682157690686168
1102	8	0.15342207230348492
1102	9	0.03497828596144359
1102	10-14	0.06449861385772948
1102	15-19	-0.003678858073733693
1102	20-24	0.06107188476480019
1102	25-29	0.0965587516744435
1102	30-34	-0.03222007191865828
1102	35-39	0.13653050515134169
1102	40-44	0.19058524247615338
1102	45-49	0.08025673559024682
1102	50-54	0.1267658139577179
1102	55-59	-0.0748059158094776
1102	60-64	0.10098743964743306
1102	65-69	0.04626443151753534
1102	70-74	-0.005583959362880364
1102	75-79	-0.047589040481568645
1102	80-84	0.05243382832103549
1102	85-89	0.047576526211749126
1102	90-94	-0.15720329772894104
1102	95-99	-0.042498588864901876
1102	100-104	0.060382574125469546
1102	105-109	-0.03516971302813943
1102	110-114	-0.13430504213447847
1102	115-119	0.011562653663041544
1102	120-124	-0.20936530498227768
1102	125-129	-0.0788437649850735
1102	130-134	-0.06153638934876682
1102	135-139	-0.11702372074596212
1102	140-144	0.05207385024352362
1102	145-149	-0.09557849465179657
1102	150-154	-0.14913706806145655
1102	155-159	-0.13750457948055583
1102	160-164	-0.07520975706923849
1102	165-169	-0.006397658285877128
1102	170-174	-0.18050957167265835
1102	175-179	-0.12624557352373245
1102	180-184	-0.29846007879703507
1102	185-189	0.10501542347417114
1102	190-194	-0.1749788037490987
1102	195-199	-0.4699881653550051
1102	200-204	0.07978043690627601
1102	205-209	-0.07198156648260579
1102	210-214	-0.029858280118020275
1102	215-219	-0.05490825846855785
1102	220-224	-0.16529121201236308
1102	225-229	-0.048961171379716006
1102	230-234	-0.2114688387416237
1102	235-239	-0.1342637586930735
1102	240-244	-0.13098360762930383
1102	245-249	-0.08958230463142769
1102	250-254	-0.3646885035631655
1102	255-259	-0.5558047185137234
1102	260-264	-0.22176838015242595
1102	265-269	-0.0877198507508794
1102	270-274	-0.31425523516826104
1102	275-279	-0.32805209896813636
1102	280-284	-0.5773261273218253
1102	285-289	-0.9708800482552036
1102	290-294	-0.8369092594552043
1102	295-299	-1.0262156342426465
1102	300-301	-1.031404983653772
1103	1	0.06778870386394686
1103	2	-0.06699444588227976
1103	3	-0.556615247601222
1103	4	0.051440019446900465
1103	5	-0.23932645417994536
1103	6	-0.24170406370303965
1103	7	-0.28091574457902624
1103	8	0.0839431641148991
1103	9	0.02008994849246193
1103	10-14	-0.22334257720927297
1103	15-19	-0.09598655038142567
1103	20-24	0.05015377062088078
1103	25-29	-0.014607501427292391
1103	30-34	-0.11261709425116351
1103	35-39	-0.09225361395535714
1103	40-44	-0.24514676744940544
1103	45-49	-0.024457904608269132
1103	50-54	-0.02956172946660729
1103	55-59	-0.18845355848937118
1103	60-64	-0.042933155886068164
1103	65-69	-0.146291399747966
1103	70-74	-0.1653854481966306
1103	75-79	-0.07190665834756516
1103	80-84	0.0633519424649549
1103	85-89	-0.022894937808096927
1103	90-94	-0.07482116373389402
1103	95-99	0.05923838383981206
1103	100-104	0.1174545344232314
1103	105-109	-0.17412752940531107
1103	110-114	0.04534756332458301
1103	115-119	-0.10704776817319583
1103	120-124	-0.05899309654555651
1103	125-129	0.011975093575721019
1103	130-134	-0.06083271170719229
1103	135-139	0.007407473160434108
1103	140-144	-0.1646650359705717
1103	145-149	-0.005528743408007131
1103	150-154	-0.07650025214104517
1103	155-159	-0.1534249774905021
1103	160-164	-0.06774707050207951
1103	165-169	-0.1302782553008015
1103	170-174	-0.19195235774230923
1103	175-179	-0.10385751382224129
1103	180-184	-0.20990286486668452
1103	185-189	0.01247811004133581
1103	190-194	-0.19985442563964995
1103	195-199	-0.237649856897292
1103	200-204	0.173513765276887
1103	205-209	-0.023622364983857835
1103	210-214	0.03909247731674981
1103	215-219	-0.10719545093515137
1103	220-224	-0.270178248153357
1103	225-229	-0.05294747427919333
1103	230-234	-0.22658651718234069
1103	235-239	-0.36945438908226436
1103	240-244	-0.3242913829670684
1103	245-249	-0.19200535149189335
1103	250-254	-0.31673996694451745
1103	255-259	-0.565415021115431
1103	260-264	-0.4656226747641554
1103	265-269	-0.30161049726891065
1103	270-274	-0.3707135917643285
1103	275-279	-0.1215472168947116
1103	280-284	-0.46943954402881616
1103	285-289	-0.521143729847239
1103	290-294	-0.44342418482834134
1103	295-299	-0.38685144032208996
1103	300-301	-0.6552780744938218
1104	1	0.017220532387185017
1104	2	0.27932017490193317
1104	3	-0.15355628274716793
1104	4	-0.13837718283185296
1104	5	0.24025516776306688
1104	6	0.06822088791290071
1104	7	-0.04531114096432276
1104	8	0.15880953916268226
1104	9	0.11673673572999377
1104	10-14	-0.020397671338265866
1104	15-19	0.1437739257541537
1104	20-24	0.09325714567472687
1104	25-29	0.08547682941656376
1104	30-34	0.004824829705469824
1104	35-39	0.15516094797928304
1104	40-44	-0.027989384253693572
1104	45-49	0.03043358768365323
1104	50-54	0.13131603934554192
1104	55-59	0.05284792731089283
1104	60-64	0.08441369869479587
1104	65-69	0.06857509663915096
1104	70-74	-0.011738290874106383
1104	75-79	0.07487633230323354
1104	80-84	-0.02958428764296883
1104	85-89	0.09680511133433356
1104	90-94	-0.2748053896192246
1104	95-99	-0.11521119056244089
1104	100-104	0.06525556216746509
1104	105-109	0.05611630200581175
1104	110-114	-0.13177633021228274
1104	115-119	-0.2447582160973809
1104	120-124	0.0694361049808947
1104	125-129	-0.005319403078345886
1104	130-134	-0.0195153612405079
1104	135-139	0.047944397341481704
1104	140-144	0.0818651492889515
1104	145-149	-0.007601509163585263
1104	150-154	-0.1274265497483711
1104	155-159	0.009327275912404787
1104	160-164	-0.035739626061683794
1104	165-169	-0.005807698549112672
1104	170-174	-0.23722598958310215
1104	175-179	-0.07301904114893176
1104	180-184	-0.015368203211636455
1104	185-189	-0.08611936712053136
1104	190-194	-0.07133078467575871
1104	195-199	-0.36774145270796765
1104	200-204	0.20322920772212427
1104	205-209	-0.18724875134070373
1104	210-214	-0.051239150825608704
1104	215-219	-0.14202280797043443
1104	220-224	0.007368715732766873
1104	225-229	0.06005973873961068
1104	230-234	-0.17861963731598962
1104	235-239	-0.07416571589969578
1104	240-244	-0.24683953581234874
1104	245-249	-0.04190139378226121
1104	250-254	-0.45513237223934766
1104	255-259	-0.4463276539587504
1104	260-264	-0.2606461185995599
1104	265-269	-0.08969773638384027
1104	270-274	-0.16345179515560915
1104	275-279	0.0620231969240308
1104	280-284	-0.48630446302716734
1104	285-289	-0.6976058325367305
1104	290-294	-0.5860024244371438
1104	295-299	-0.9146339503975014
1104	300-301	-1.0371707819220788
1201	1	-0.6698210324049931
1201	2	-0.021413321430586052
1201	3	0.06404762923327212
1201	4	-0.3290862292866237
1201	5	0.20602533891221242
1201	6	-0.03446859863966978
1201	7	-0.003746348788290277
1201	8	-0.09302420166861936
1201	9	0.12407169905517179
1201	10-14	0.07006687633899134
1201	15-19	-0.05231409380574803
1201	20-24	0.012572549097704666
1201	25-29	0.05369198834077338
1201	30-34	0.022428741685907028
1201	35-39	-0.028702132705319627
1201	40-44	-0.09302605907031136
1201	45-49	-0.017488172707544436
1201	50-54	0.02667056257292444
1201	55-59	0.017151105795001342
1201	60-64	-0.046637646048480974
1201	65-69	-0.04193835079361463
1201	70-74	-0.11491677498167974
1201	75-79	-0.024879168919262895
1201	80-84	-0.022738321872800782
1201	85-89	-0.019087309203563052
1201	90-94	-0.31539218668523716
1201	95-99	-0.10396424679716176
1201	100-104	-0.034988936610034216
1201	105-109	-0.13557073955897891
1201	110-114	-0.2466907556401452
1201	115-119	-0.22862129678197363
1201	120-124	-0.06406022753744622
1201	125-129	-0.13832673804166973
1201	130-134	-0.104111938257617
1201	135-139	-0.07870596940669117
1201	140-144	-0.01446736904846091
1201	145-149	0.04521022677773345
1201	150-154	-0.056032906716588116
1201	155-159	-0.14372895880642034
1201	160-164	-0.06801346469248415
1201	165-169	-0.19847273522393039
1201	170-174	-0.1751233000965513
1201	175-179	-0.20504838100223566
1201	180-184	-0.30718947939936925
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2404	270-274	0.5385327728436238
2404	275-279	0.47336031945919643
2404	280-284	0.7710695565962524
2404	285-289	0.7514666597631461
2404	290-294	0.9780238671197132
2404	295-299	0.9174726398120541
2404	300-301	1.0880440272509304
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	22.0
16	51.0
17	61.0
18	119.0
19	202.0
20	379.0
21	436.0
22	475.0
23	446.0
24	448.0
25	500.0
26	573.0
27	656.0
28	873.0
29	1264.0
30	2082.0
31	3692.0
32	10828.0
33	96351.0
34	3274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.3202072792158588	0.06925602731131807	0.08310723277358167	99.52742946069925
2	0.057036699041783455	0.17111009712535039	0.32103513460660976	99.45081806922626
3	0.18902821572030343	0.20450897476636276	99.4679507548907	0.13851205462263613
4	0.08310723277358167	99.51602258561267	0.21510107306103493	0.18576910855271198
5	0.18821343892840556	0.23791482323417498	0.2159158498529328	99.35795588798449
6	99.39869472757938	0.24117393040176643	0.0684412505194202	0.2916900914994337
7	99.80608312352831	0.07903334881409238	0.032591071675914386	0.08229245598168382
8	99.49076450506384	0.14584504574971685	0.10999486690621105	0.25339558228023434
9	30.27954991730016	22.007121149161186	25.454441755681028	22.258887177857627
10-14	59.68582206904418	10.598453553648977	9.47503931298021	20.240685064326627
15-19	22.354541973226436	37.69320394677878	19.82547481117548	20.126779268819305
20-24	58.68967596326986	39.857577016776254	1.1066298387556728	0.3461171811982107
25-29	1.6450343428417786	2.0548670691664017	59.42134552239414	36.87875306559768
30-34	2.1094571142235585	58.38478648774168	0.5868022455248385	38.91895415250992
35-39	45.27273112441193	15.585916048384863	20.024671521665802	19.1166813055374
40-44	38.764678467018875	48.37498003761077	0.8499903854615142	12.010351109908841
45-49	20.299356310600505	20.01189603194003	34.74602786604742	24.942719791412042
50-54	57.25446101197751	20.305874684266275	19.136315489285423	3.3033488144707897
55-59	17.38531736331785	41.96691925364622	1.5523506885032183	39.09541269453272
60-64	39.649451606069	0.8317986994996822	0.7302684115317547	58.78848128289956
65-69	40.38494132985658	20.727672750977835	37.61782920469361	1.2695567144719688
70-74	40.49543676662321	57.41166883963494	1.8336049543676662	0.25928943937418514
75-79	49.05785528031291	20.485332464146023	27.330019556714475	3.126792698826597
80-84	39.42323989569752	1.1518904823989569	39.20029335071708	20.22457627118644
85-89	21.302776157058062	9.469801917266151	40.1185171664097	29.10890475926608
90-94	19.238053328117868	57.908436012777884	2.016102744637851	20.837407914466393
95-99	19.412965990230767	0.8424617681429171	59.472357181623934	20.27221506000238
100-104	37.308815667661975	9.757898374036744	48.197684273662524	4.7356016846387545
105-109	40.742690611318835	43.703445330760225	14.869622477287026	0.6842415806339095
110-114	45.82625592138938	48.249583505853735	0.9580180421025865	4.966142530654295
115-119	24.512063906097165	47.72285621128138	23.07091620476035	4.694163677861102
120-124	55.72740477964372	23.816378732721112	17.755500959417535	2.700715528217635
125-129	3.701137469655792	38.088196694050495	37.463949695049884	20.74671614124383
130-134	35.928642581962066	8.293783615901187	22.665697148344456	33.11187665379229
135-139	8.873917133945211	21.867494533697045	58.940034534316645	10.318553798041098
140-144	40.14310021093089	19.148870222855905	19.548382781644936	21.159646784568274
145-149	17.960239918025835	36.77546518382185	38.29469582754097	6.9695990706113475
150-154	4.216108609098622	29.67805626264604	24.33261536453234	41.773219763723
155-159	21.565047117855833	73.62852364051727	0.7755884632643903	4.030840778362502
160-164	21.82127640131136	19.31475307644733	17.752418811327004	41.11155171091431
165-169	38.47417989780648	21.68503440993931	20.672238188259982	19.16854750399423
170-174	2.4921859060676543	40.036724201248624	19.936344717835723	37.534745174848
175-179	41.812471228153804	2.898732896055686	18.922324993715087	36.366470882075426
180-184	38.42706278817644	38.770915241839624	1.1922177666897424	21.609804203294193
185-189	39.52216958717686	1.4226158692212354	4.792347397831131	54.26286714577078
190-194	20.41185041916872	38.447213491809386	37.35681941440529	3.784116674616606
195-199	20.639609933365797	38.596204562760015	21.453011284350605	19.311174219523593
200-204	75.08320945101495	2.0134713798955444	21.235676792127663	1.6676423769618354
205-209	21.775908194299404	38.987352529249016	2.616763169205476	36.6199761072461
210-214	37.15939347480358	6.709566485184198	53.81529977296985	2.3157402670423806
215-219	39.00950705836968	19.535729931541333	3.772419069040598	37.68234394104839
220-224	5.357726660476795	20.465973139442127	72.48313041886254	1.693169781218533
225-229	56.49010443996541	1.5295738869027478	21.618206457329027	20.362115215802817
230-234	20.370891067096498	57.04542630894075	20.586023579569606	1.99765904439315
235-239	56.62996883899931	20.150344471292968	2.639039207928562	20.580647481779152
240-244	38.806548499806254	21.8450641819035	9.496730818595175	29.85165649969507
245-249	54.697036832355316	11.346955326033463	12.94716068599383	21.008847155617396
250-254	15.573468937269555	38.516477676856034	32.73347122640352	13.176582159470884
255-259	21.742059436700323	36.29873686792299	19.82749458365695	22.13170911171974
260-264	38.81994737952314	19.891455958557696	37.50705176527146	3.7815448966476928
265-269	21.766324743557668	56.648532150747045	18.40023808706203	3.184905018633256
270-274	20.46408568742079	2.2502759494705855	31.2069007808348	46.078737582273824
275-279	38.23102308072202	5.293161473476957	19.728833404741735	36.74698204105928
280-284	2.878537868913851	38.71651422506627	13.31892047470854	45.08602743131134
285-289	51.79572218924219	41.24592587504763	3.8423107177492026	3.116041217960983
290-294	28.526898514224037	20.212277072277235	24.392074771246104	26.86874964225262
295-299	32.995738230922925	27.789588685150733	19.676479562209035	19.538193521717307
300-301	5.881911151723015	93.14048241935686	0.8031977702395406	0.17440865868058597
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.25
24	1.0
25	0.75
26	1.75
27	1.5
28	1.0
29	0.75
30	2.5
31	2.0
32	3.833333333333333
33	2.9166666666666665
34	2.25
35	1.9166666666666665
36	2.5833333333333335
37	6.916666666666666
38	5.25
39	4.666666666666666
40	10.25
41	11.91666666666667
42	9.083333333333332
43	18.916666666666664
44	30.999999999999982
45	202.7500000000012
46	484.16666666665793
47	154.0
48	98.16666666666663
49	235.66666666666677
50	8640.41666666665
51	7612.749999999964
52	11150.083333333354
53	2761.0000000000036
54	2590.4166666666674
55	5647.749999999997
56	1146.5000000000005
57	175.66666666666674
58	82.3333333333333
59	51.50000000000003
60	29.749999999999993
61	16.75
62	10.083333333333334
63	6.749999999999999
64	7.666666666666665
65	5.083333333333334
66	5.5
67	6.5
68	6.833333333333333
69	9.333333333333336
70	12.833333333333332
71	8.75
72	1.5
73	1.0
74	2.5
75	1.75
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.25
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002444270629644114
2	0.006518055012384304
3	0.002444270629644114
4	0.002444270629644114
5	0.002444270629644114
6	0.002444270629644114
7	0.002444270629644114
8	0.002444270629644114
9	0.002444270629644114
10-14	0.002444270629644114
15-19	0.002444270629644114
20-24	0.002444270629644114
25-29	0.002444270629644114
30-34	0.002444270629644114
35-39	4.888652785065491E-4
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.6302689223659678
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-39	4.0
40-49	2.0
50-59	0.0
60-69	10.0
70-79	0.0
80-89	8.0
90-99	1.0
100-109	12.0
110-119	19.0
120-129	5.0
130-139	23.0
140-149	84.0
150-159	5.0
160-169	15.0
170-179	35.0
180-189	40.0
190-199	80.0
200-209	15.0
210-219	27.0
220-229	7.0
230-239	15.0
240-249	8.0
250-259	11.0
260-269	4.0
270-279	7.0
280-289	3.0
290-299	2083.0
300-302	120213.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	5.824696910441924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.742901105049654	3.59633685308304
2	11.931738704713945	1.389975231390953
3	6.308574625821793	1.1023660539694955
4	4.014547489159323	0.9353408942771477
5	2.825570009791579	0.8229044453135185
6	1.70653238215135	0.5964020336331638
7	1.692544411805847	0.6900990744361882
8	1.1889774793677437	0.5540346760526659
9	1.0211218352217093	0.535295267892061
>10	6.532382151349839	6.576717507495763
>50	0.2517834662190516	1.006224742536827
>100	0.44761505105609173	6.2703689219137
>500	0.0	0.0
>1k	0.2657714365645545	52.86712944857255
>5k	0.06993985172751434	23.05680484943293
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	6551	5.3374722982661975	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	5967	4.861654282362143	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5366	4.371985399556772	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5229	4.260363707469691	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	5186	4.2253291617781255	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4997	4.071340112110546	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4923	4.011048103245991	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4832	3.9369052274801195	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4817	3.9246838743318997	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4776	3.89127884239343	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4651	3.789434232824925	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4378	3.5670056055273105	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	4288	3.4936774866379876	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	4018	3.273693129970017	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGCAAGTCTGAT	3587	2.9225329161778126	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	3366	2.742471646460696	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2632	2.144440099074436	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2225	1.8128340503193847	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2136	1.7403206883066094	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2109	1.7183222526398125	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	2089	1.7020271151088517	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1847	1.5048559509842263	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1783	1.4527115108851518	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGGCGCAAGCCTGAT	1433	1.1675466040933387	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	466	0.37967670447138574	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	371	0.3022748011993221	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	360	0.29331247555729373	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	344	0.2802763655325251	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	344	0.2802763655325251	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	326	0.26561074175466043	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	325	0.26479598487811234	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	293	0.23872376482857513	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	293	0.23872376482857513	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	288	0.23464998044583496	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	282	0.22976143918654676	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	281	0.2289466823099987	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	247	0.20124494850736538	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	235	0.19146786598878895	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	231	0.18820883848259679	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	228	0.18576456785295267	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	227	0.18494981097640464	No Hit
CCTACGGGGGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	213	0.1735432147047321	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	203	0.16539564593925174	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	200	0.16295137530960763	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	189	0.1539890496675792	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	184	0.149915265284839	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	183	0.14910050840829095	No Hit
CCTACGGGGGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	181	0.1474709946551949	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	178	0.14502672402555078	No Hit
CCTACGGGTGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	172	0.14013818276626255	No Hit
CCTACGGGTGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	168	0.13687915526007038	No Hit
CCTACGGGAGGCAGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	163	0.13280537087733021	No Hit
CCTACGGGCGGCTGCAGTAATTAACCTTAAGCAATAAGTGTAAACTTGAC	158	0.12873158649459002	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	135	0.10999217833398513	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	6.518055012384304E-4	0.0	0.0	0.0
160-164	0.0	0.0016295137530960764	0.0	0.0	0.0
165-169	0.0	0.0024442706296441143	0.0	0.0	0.0
170-174	0.0	0.0024442706296441143	0.0	0.0	0.0
175-179	0.0	0.0024442706296441143	0.0	0.0	0.0
180-184	0.0	0.0024442706296441143	0.0	0.0	0.0
185-189	0.0	0.0024442706296441143	0.0	0.0	0.0
190-194	0.0	0.0024442706296441143	0.0	0.0	0.0
195-199	0.0	0.0024442706296441143	0.0	0.0	0.0
200-204	0.0	0.0024442706296441143	0.0	0.0	0.0
205-209	0.0	0.0024442706296441143	0.0	0.0	0.0
210-214	0.0	0.0024442706296441143	0.0	8.147568765480382E-4	0.0
215-219	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
220-224	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
225-229	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
230-234	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
235-239	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
240-244	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
245-249	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
250-254	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
255-259	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
260-264	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
265-269	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
270-274	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
275-279	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
280-284	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
285-289	0.0	0.0024442706296441143	0.0	0.0016295137530960764	0.0
290	0.0	0.0024442706296441143	0.0	0.0016295137530960761	0.0
>>END_MODULE
