##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DAMS2_R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80819
Sequences flagged as poor quality	0
Sequence length	44-301
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.061161360571155	40.0	40.0	40.0	40.0	40.0
2	38.598893824471965	40.0	40.0	40.0	40.0	40.0
3	39.18603298729259	40.0	40.0	40.0	40.0	40.0
4	39.333226097823534	40.0	40.0	40.0	40.0	40.0
5	37.898711936549574	40.0	40.0	40.0	24.0	40.0
6	38.97261782501639	40.0	40.0	40.0	40.0	40.0
7	38.43269528205001	40.0	40.0	40.0	40.0	40.0
8	38.703077246687045	40.0	40.0	40.0	40.0	40.0
9	39.267152526014925	40.0	40.0	40.0	40.0	40.0
10-14	39.049213675002164	40.0	40.0	40.0	36.8	40.0
15-19	38.99339511748475	40.0	40.0	40.0	40.0	40.0
20-24	39.11669285687772	40.0	40.0	40.0	40.0	40.0
25-29	39.115851470570036	40.0	40.0	40.0	40.0	40.0
30-34	38.429131763570446	40.0	40.0	40.0	36.8	40.0
35-39	39.18300399658496	40.0	40.0	40.0	40.0	40.0
40-44	39.05200509781116	40.0	40.0	40.0	40.0	40.0
45-49	38.93266351555347	40.0	40.0	40.0	40.0	40.0
50-54	39.15096141948575	40.0	40.0	40.0	40.0	40.0
55-59	38.92055730159124	40.0	40.0	40.0	40.0	40.0
60-64	38.525602621151265	40.0	40.0	40.0	33.6	40.0
65-69	38.81755571228827	40.0	40.0	40.0	40.0	40.0
70-74	38.25368116023446	40.0	40.0	40.0	33.6	40.0
75-79	38.804890120768164	40.0	40.0	40.0	40.0	40.0
80-84	38.57949910908731	40.0	40.0	40.0	36.8	40.0
85-89	38.96781836993596	40.0	40.0	40.0	40.0	40.0
90-94	38.9416720289066	40.0	40.0	40.0	40.0	40.0
95-99	38.48064148888779	40.0	40.0	40.0	36.8	40.0
100-104	39.024916834756596	40.0	40.0	40.0	40.0	40.0
105-109	38.82560542500403	40.0	40.0	40.0	40.0	40.0
110-114	39.054923072225556	40.0	40.0	40.0	40.0	40.0
115-119	39.052746033500256	40.0	40.0	40.0	40.0	40.0
120-124	38.37996188401851	40.0	40.0	40.0	36.8	40.0
125-129	38.862258523606215	40.0	40.0	40.0	40.0	40.0
130-134	38.73616731637894	40.0	40.0	40.0	36.8	40.0
135-139	38.74192933605594	40.0	40.0	40.0	36.8	40.0
140-144	38.70319417433693	40.0	40.0	40.0	40.0	40.0
145-149	38.639271305165714	40.0	40.0	40.0	40.0	40.0
150-154	38.45785995396154	40.0	40.0	40.0	36.8	40.0
155-159	38.85171697169742	40.0	40.0	40.0	40.0	40.0
160-164	38.52574257425742	40.0	40.0	40.0	36.8	40.0
165-169	38.82876237623763	40.0	40.0	40.0	36.8	40.0
170-174	38.92269306930693	40.0	40.0	40.0	40.0	40.0
175-179	38.951534653465345	40.0	40.0	40.0	40.0	40.0
180-184	38.51609366542021	40.0	40.0	40.0	33.6	40.0
185-189	39.002694357210125	40.0	40.0	40.0	40.0	40.0
190-194	38.466733465711705	40.0	40.0	40.0	33.6	40.0
195-199	39.07097650552624	40.0	40.0	40.0	40.0	40.0
200-204	38.568430967248354	40.0	40.0	40.0	40.0	40.0
205-209	38.78522779713812	40.0	40.0	40.0	40.0	40.0
210-214	38.608456419355825	40.0	40.0	40.0	40.0	40.0
215-219	38.68203340930946	40.0	40.0	40.0	40.0	40.0
220-224	38.63570897924818	40.0	40.0	40.0	36.8	40.0
225-229	38.56988416271378	40.0	40.0	40.0	40.0	40.0
230-234	38.85502024441886	40.0	40.0	40.0	40.0	40.0
235-239	38.43408491512203	40.0	40.0	40.0	36.8	40.0
240-244	38.47586181715919	40.0	40.0	40.0	36.8	40.0
245-249	37.5172734503054	40.0	40.0	40.0	34.4	40.0
250-254	38.60794566060422	40.0	40.0	40.0	40.0	40.0
255-259	38.35078569040455	40.0	40.0	40.0	33.6	40.0
260-264	37.93032430625209	40.0	40.0	40.0	33.6	40.0
265-269	36.9923032161753	40.0	36.8	40.0	27.2	40.0
270-274	38.45232459324606	40.0	40.0	40.0	40.0	40.0
275-279	37.611422344404	40.0	40.0	40.0	30.4	40.0
280-284	38.204522376600536	40.0	40.0	40.0	36.8	40.0
285-289	38.23886666749223	40.0	40.0	40.0	36.8	40.0
290-294	37.78703769547615	40.0	40.0	40.0	33.6	40.0
295-299	38.152509939490585	40.0	40.0	40.0	36.8	40.0
300-301	33.36004259953072	40.0	26.0	40.0	18.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.17571213208766778
1101	2	-0.15927258820489243
1101	3	-0.22727293604462773
1101	4	-0.2583595884309773
1101	5	-0.619858459683357
1101	6	-0.14518118869283825
1101	7	-0.41014932537210314
1101	8	-0.23759879049176647
1101	9	-0.37399532707936345
1101	10-14	-0.2080390008775126
1101	15-19	-0.22116751243486021
1101	20-24	-0.2151705389267704
1101	25-29	-0.22263292718287886
1101	30-34	-0.13893442713701631
1101	35-39	-0.22656554661876527
1101	40-44	-0.2651747660787649
1101	45-49	-0.35977852257066445
1101	50-54	-0.44254877889099475
1101	55-59	-0.3669578084964584
1101	60-64	-0.46258188817449053
1101	65-69	-0.4331583441647311
1101	70-74	-0.4537201625380334
1101	75-79	-0.3455624273832214
1101	80-84	-0.21635776364882275
1101	85-89	-0.23515071928077447
1101	90-94	-0.39217270264389725
1101	95-99	-0.16025355247808193
1101	100-104	-0.44585469438997194
1101	105-109	-0.053953404000310456
1101	110-114	-0.1530954405776157
1101	115-119	-0.15817829554868013
1101	120-124	-0.3618741571015178
1101	125-129	-0.4023113436336416
1101	130-134	-0.4213901904226631
1101	135-139	-0.20235719601411972
1101	140-144	-0.21648229570423183
1101	145-149	-0.262000745126457
1101	150-154	-0.17397063804428825
1101	155-159	-0.06499120628507882
1101	160-164	-0.38803866038103507
1101	165-169	-0.004962317392049442
1101	170-174	-0.2532190901880611
1101	175-179	-0.09521892020826783
1101	180-184	-0.0941177764043033
1101	185-189	-0.07744168541793783
1101	190-194	-0.14650420896374072
1101	195-199	-0.19431193113458534
1101	200-204	-0.3196423819351324
1101	205-209	-0.22321180616802394
1101	210-214	0.04441302242315004
1101	215-219	-0.41731716206020764
1101	220-224	-0.24592471995073595
1101	225-229	-0.3828148271544336
1101	230-234	-0.40120890456687164
1101	235-239	-0.2082782603158435
1101	240-244	-0.2668354540265838
1101	245-249	0.5002918820938191
1101	250-254	-0.18872446717883662
1101	255-259	-0.5348457923804943
1101	260-264	-0.6353065452005353
1101	265-269	-0.4532925552671401
1101	270-274	-0.4116311172548208
1101	275-279	-0.6169181455212396
1101	280-284	-0.38268213137224905
1101	285-289	-0.34989175260412253
1101	290-294	-0.5961562797177464
1101	295-299	-0.504469838715309
1101	300-301	-2.0074055217359046
1102	1	-0.21277770545876962
1102	2	0.33050667893286345
1102	3	0.1518922129318412
1102	4	-0.12350127998776372
1102	5	-0.12471749661786902
1102	6	-0.07874278263178525
1102	7	-0.038618765162297564
1102	8	0.23883451613021833
1102	9	-0.027466588097794897
1102	10-14	-0.2343346226448304
1102	15-19	-0.2269604752089478
1102	20-24	-0.1925465132838724
1102	25-29	-0.30558768787349067
1102	30-34	-0.19853084295984758
1102	35-39	-0.13514863105091024
1102	40-44	-0.1224953750974791
1102	45-49	-0.44768702406407357
1102	50-54	-0.3973706627694824
1102	55-59	-0.2790842745851876
1102	60-64	-0.5551527339528803
1102	65-69	-0.36134658631524275
1102	70-74	-0.3805213572637598
1102	75-79	-0.24132406503164816
1102	80-84	-0.4853633730317881
1102	85-89	-0.2328661705811257
1102	90-94	-0.41128831421234224
1102	95-99	-0.3461412191204616
1102	100-104	-0.3170574335172418
1102	105-109	-0.27891151575087747
1102	110-114	-0.3562337810744438
1102	115-119	-0.28444623464680774
1102	120-124	-0.23730797366741285
1102	125-129	-0.4796829470429813
1102	130-134	-0.5550479452125003
1102	135-139	-0.6085522859827961
1102	140-144	-0.27441192344518583
1102	145-149	-0.4276072141291536
1102	150-154	-0.4041505753940342
1102	155-159	-0.41828034447986795
1102	160-164	-0.6431971072375902
1102	165-169	-0.4013548285015247
1102	170-174	-0.34457772645235707
1102	175-179	-0.25644278558307576
1102	180-184	-0.3842210234886849
1102	185-189	-0.4685145764908256
1102	190-194	-0.5481656106251407
1102	195-199	-0.5638217006443682
1102	200-204	-0.5774755397682938
1102	205-209	-0.35514113809735903
1102	210-214	-0.11927014126001012
1102	215-219	-0.4929694377124889
1102	220-224	-0.34709472112073314
1102	225-229	-0.582838227177831
1102	230-234	-0.44187814523611024
1102	235-239	-0.5224703745079609
1102	240-244	-0.5753764625675828
1102	245-249	-0.11463733283539312
1102	250-254	-1.03662431507869
1102	255-259	-0.7646223221570239
1102	260-264	-0.801130811024791
1102	265-269	-1.3566972586718435
1102	270-274	-1.0196807253044327
1102	275-279	-1.0473498759529676
1102	280-284	-0.9571176058077242
1102	285-289	-1.4206300233423903
1102	290-294	-1.149718933280397
1102	295-299	-1.1175828658361127
1102	300-301	-1.1363291504229238
1103	1	-0.06562896351449155
1103	2	-0.8949673346452371
1103	3	-0.3318671704557943
1103	4	-0.0673516624998527
1103	5	-0.8782017296560056
1103	6	0.06496119750342189
1103	7	-0.6932383342800037
1103	8	-0.2906253739244704
1103	9	-0.49233719423234135
1103	10-14	-0.3517550701768073
1103	15-19	-0.14409098862198988
1103	20-24	-0.24753639429605556
1103	25-29	-0.21597795068010583
1103	30-34	-0.5784995530400892
1103	35-39	-0.10779722228685529
1103	40-44	-0.05142993897629111
1103	45-49	-0.2914555934832066
1103	50-54	-0.028425190478280626
1103	55-59	-0.2241058761123682
1103	60-64	-0.4973381482572563
1103	65-69	-0.13030641178064428
1103	70-74	-0.23998655799289992
1103	75-79	-0.3113560726101312
1103	80-84	-0.712832477824314
1103	85-89	-0.2643742585947919
1103	90-94	-0.30654459013775437
1103	95-99	-0.5762513079666078
1103	100-104	-0.4789612385437181
1103	105-109	-0.3844016048840899
1103	110-114	-0.08714047108893652
1103	115-119	-0.175270250377892
1103	120-124	-0.4904061063194405
1103	125-129	-0.35363911244896684
1103	130-134	-0.5398106154770304
1103	135-139	-0.4729779437169981
1103	140-144	-0.30519661147894794
1103	145-149	-0.5452917600664975
1103	150-154	-0.4049084229920368
1103	155-159	-0.5390881411240969
1103	160-164	-0.7298328667358049
1103	165-169	-0.2947624155894175
1103	170-174	-0.5048624934285755
1103	175-179	-0.4328720029044888
1103	180-184	-0.7009209416755908
1103	185-189	-0.35815589066746156
1103	190-194	-0.5012936278712203
1103	195-199	-0.26803422873747706
1103	200-204	-0.2946310748342782
1103	205-209	-0.5665858450644521
1103	210-214	-0.3096349196844912
1103	215-219	-0.8519621190932156
1103	220-224	-0.22561716680736055
1103	225-229	-0.9451847954945549
1103	230-234	-0.5106616408852744
1103	235-239	-0.6125297302389541
1103	240-244	-0.7542619578709875
1103	245-249	0.09034163333760148
1103	250-254	-0.53616906238463
1103	255-259	-0.6849588633890917
1103	260-264	-0.5387439038617714
1103	265-269	-1.1840930980079776
1103	270-274	-0.7922733605836427
1103	275-279	-0.8686142106501435
1103	280-284	-0.9517006750176478
1103	285-289	-0.8211717164214036
1103	290-294	-0.9908645565155751
1103	295-299	-0.988910759748542
1103	300-301	-5.07707485142361
1104	1	-0.30162483613099766
1104	2	-0.17086595932190818
1104	3	0.023935902466028836
1104	4	0.11544054070310494
1104	5	-0.22918481021065418
1104	6	0.10476500543873613
1104	7	-0.1665163531359326
1104	8	-0.26371257196819897
1104	9	0.16561296619848775
1104	10-14	0.08446978741432787
1104	15-19	0.08009943641370398
1104	20-24	0.06297853919935648
1104	25-29	-0.07850781060349732
1104	30-34	-0.1361910452588333
1104	35-39	0.14007194531035339
1104	40-44	0.0513701797566668
1104	45-49	-0.16354062889106302
1104	50-54	0.13806102095117012
1104	55-59	-0.05522238988680783
1104	60-64	-0.18777307836030133
1104	65-69	-0.049635853311727374
1104	70-74	-0.3272835458510386
1104	75-79	-0.08016605748581185
1104	80-84	-0.14648761514462194
1104	85-89	-0.13807632370002665
1104	90-94	-0.21391027348828828
1104	95-99	-0.10264253303622439
1104	100-104	0.22811043940729547
1104	105-109	0.17739748200298777
1104	110-114	0.003288805064116218
1104	115-119	0.10151321747233766
1104	120-124	-0.10906979523911531
1104	125-129	-0.28879961395433185
1104	130-134	-0.3425782239975348
1104	135-139	0.06988041070085416
1104	140-144	-0.029668320526674563
1104	145-149	-0.23119222227690273
1104	150-154	0.04451275387765463
1104	155-159	-0.07956900957081103
1104	160-164	-0.07027698450757924
1104	165-169	-0.16854363599451716
1104	170-174	-0.08954594290347018
1104	175-179	0.06985461447857944
1104	180-184	-0.13477962119556963
1104	185-189	-0.08146288601708562
1104	190-194	-0.4202915116483794
1104	195-199	0.01762492795056403
1104	200-204	-0.012706098953223943
1104	205-209	-0.21330283047958432
1104	210-214	0.3285240951273778
1104	215-219	-0.14385247718867333
1104	220-224	-0.24273310801387993
1104	225-229	-0.34373198350885303
1104	230-234	-0.160397751208194
1104	235-239	-0.293887201438082
1104	240-244	-0.36775721920690074
1104	245-249	0.4077697021191682
1104	250-254	0.05796293920437989
1104	255-259	-0.3391896107927579
1104	260-264	-0.6059137580130738
1104	265-269	-0.8952559151012238
1104	270-274	-0.48272225678702796
1104	275-279	-0.6067902506024723
1104	280-284	-0.5791679016222773
1104	285-289	-0.8503641572590297
1104	290-294	-0.5336260342977681
1104	295-299	-0.5109817551891922
1104	300-301	-2.9955270768395152
1201	1	0.29646684906879983
1201	2	-0.06870655046904517
1201	3	-0.2725559549125549
1201	4	0.028432864399213997
1201	5	0.059386823335508154
1201	6	-0.2357472264286855
1201	7	-0.0176964951834222
1201	8	-0.26778746973705125
1201	9	0.003363163486667986
1201	10-14	-0.008793717858644357
1201	15-19	-0.1366392105480685
1201	20-24	-0.05215167100223539
1201	25-29	-0.09282160642815285
1201	30-34	-0.009123106382304513
1201	35-39	-0.16015045227914726
1201	40-44	-0.09913703022970566
1201	45-49	-0.18166531502350125
1201	50-54	-0.17085066568344587
1201	55-59	-0.10129743113797218
1201	60-64	-0.48069509572165714
1201	65-69	0.007596372816394137
1201	70-74	-0.20315412480218242
1201	75-79	-0.1372605405907663
1201	80-84	-0.07145210327146145
1201	85-89	-0.16873562494114225
1201	90-94	-0.011795344153327392
1201	95-99	0.0691804097860782
1201	100-104	-0.23755280759751685
1201	105-109	-0.20187793230219597
1201	110-114	-0.14403883680402174
1201	115-119	-0.046480182341134935
1201	120-124	-0.017723213705288288
1201	125-129	-0.037028324765721266
1201	130-134	-0.10139019042266995
1201	135-139	-0.054432667712234206
1201	140-144	-0.23459550325140555
1201	145-149	-0.15332149984343602
1201	150-154	-0.6177442229499519
1201	155-159	-0.31253837609639135
1201	160-164	-0.12539715094706594
1201	165-169	-0.6117547702222339
1201	170-174	-0.34378512792391547
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2404	280-284	0.3969514133775789
2404	285-289	0.5208622995452714
2404	290-294	0.7723426631997938
2404	295-299	0.5984650216998659
2404	300-301	5.052393994337372
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	35.0
16	181.0
17	173.0
18	120.0
19	111.0
20	89.0
21	114.0
22	120.0
23	116.0
24	127.0
25	153.0
26	167.0
27	203.0
28	246.0
29	289.0
30	356.0
31	402.0
32	478.0
33	580.0
34	799.0
35	1158.0
36	1716.0
37	3123.0
38	8617.0
39	60484.0
40	862.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.75377077172448	0.20910924411338916	0.019797324886474715	0.017322659275665377
2	0.6594983852806889	99.03488041178436	0.018559992081070044	0.28706121085388336
3	0.30685853574035804	0.18683725361610512	0.21900790655662652	99.28729630408691
4	0.28087454682686	0.16085326470260705	99.21676833417884	0.34150385429168884
5	0.36130117917816357	97.76413962063376	0.10888528687561093	1.7656739133124637
6	0.19426125044853312	0.32913052623764216	0.16456526311882108	99.312042960195
7	0.3909971665078756	31.236466672440887	37.33899206869672	31.03354409235452
8	34.614385231195634	33.28425246538561	0.14847993664856035	31.95288236677019
9	99.62137616154617	0.2128212425296032	0.06310397307563816	0.10269862284858758
10-14	40.074487434885356	19.719620386295304	39.75575050421312	0.4501416746062188
15-19	0.24226976329823433	39.55505512317648	39.690666798648834	20.51200831487645
20-24	19.93912322597409	0.38258330343112384	39.48774421856246	40.19054925203232
25-29	20.221482572167435	0.31700466474467637	41.21308108241874	38.24843168066915
30-34	0.475630730397555	37.687177520137595	0.8438609732859848	60.993330776178865
35-39	19.916851235476805	0.5122557814375333	57.93736621339042	21.633526769695244
40-44	39.529071134262985	19.118276642868633	20.56620349175318	20.786448731115208
45-49	1.1898339478828974	19.828751020812195	20.164567299364002	58.8168477319409
50-54	39.05788314484397	1.2997104605409686	20.44123833799401	39.20116805662105
55-59	38.42906283253731	20.850058155361427	39.58895295602465	1.1319260560766164
60-64	20.579777572100987	37.41777740381998	2.2091831936765933	39.793261830402436
65-69	39.34642463838054	39.746093024982365	0.6872316468069837	20.22025068983011
70-74	40.466833628651464	19.514011938111878	0.5763653101830313	39.44278912305362
75-79	0.6911997624232825	0.39299148683429025	39.99653533953673	58.919273411205694
80-84	20.261334389229855	20.06632350029697	0.8384478321124531	58.833894278360724
85-89	59.26954335323058	0.4325918886546095	39.60789554440254	0.6899692137122719
90-94	0.8107706776221354	0.2546651487402861	77.36821264168687	21.5663515319507
95-99	0.8929366925704103	39.17536999455527	20.101717566698017	39.82997574617631
100-104	0.5481913398142831	19.831162017146138	58.879462252757044	20.741184390282534
105-109	20.06286272908391	39.29168058803876	1.054064421922758	39.59139226095457
110-114	0.37793007179928867	20.04242122744361	40.50212477384833	39.07752392690877
115-119	20.259584041818574	0.5437701562503867	20.050936685551356	59.145709116379685
120-124	0.4982303294309829	57.73531668440462	1.6107714773655424	40.15568150879885
125-129	57.845430357032356	22.562217684549225	19.22999814367923	0.36235381473918693
130-134	0.7200049501887259	19.270094672359384	40.054699585421695	39.9552007920302
135-139	0.8930140461605099	0.7427758183280737	38.97778602809232	59.3864241074191
140-144	0.5388440470974943	0.7462631152957949	57.69641075905974	41.018482078546974
145-149	20.029454716467416	20.81136605529566	19.62698943095468	39.53218979728225
150-154	0.8477512932848198	39.01066805277097	20.388604242469246	39.75297641147497
155-159	20.350536745468542	19.987921061164478	38.87017368424961	20.791368509117373
160-164	20.15891089108911	38.34950495049505	20.776237623762377	20.715346534653463
165-169	18.989356435643565	2.7094059405940594	57.448762376237624	20.85247524752475
170-174	21.017326732673265	39.06955445544554	19.67871287128713	20.234405940594062
175-179	1.3462871287128713	19.411881188118812	57.280693069306935	21.961138613861387
180-184	40.28684020317435	1.1242908205194213	21.276374545778587	37.312494430527636
185-189	40.01277351031411	19.90934768455371	19.643232886342986	20.43464591878919
190-194	40.078578165964814	37.406821495856946	1.53219996583558	20.982400372342664
195-199	19.52529263800036	0.9858583057695736	59.543662976093806	19.945186080136267
200-204	0.5991448568860808	38.84612623140361	19.70740934220332	40.84731956950699
205-209	20.45942406108488	38.49001106717802	1.8710215723315597	39.17954329940554
210-214	2.0284450914021646	20.589041710494175	75.27380768406307	2.108705514040589
215-219	19.550897907029725	39.25086918192894	0.8974117693671563	40.30082114167418
220-224	37.62369372492695	1.7131395176068547	20.597295824872468	40.065870932593725
225-229	2.1613804306912057	19.367484200725084	19.20156705033976	59.26956831824395
230-234	18.876713346458153	0.8456842861211199	59.23058826442802	21.047014102992705
235-239	19.59486398474549	19.810556814382824	0.6136473385089707	59.980931862362716
240-244	1.7565235049243577	58.61261140786858	20.10604023189181	19.52482485531526
245-249	1.3037408070367669	39.3096535897054	21.06283657038408	38.32376903287376
250-254	38.76186891075966	40.51576825348081	1.6687057005443477	19.05365713521519
255-259	19.859083423108835	19.75803955075102	20.696657874859145	39.686219151281
260-264	19.84323340391545	19.49552360786062	19.60994093391285	41.05130205431109
265-269	1.0624492302815365	20.09465456778872	20.410665108077588	58.43223109385216
270-274	19.466438157695958	20.771614034479427	59.17251354089015	0.5894342669344713
275-279	20.650370260296704	19.59902915025881	0.8715357753176313	58.879064814126856
280-284	58.53381875820393	0.5015231443643658	20.164697723951754	20.79996037347995
285-289	39.78428046016867	1.0441717335578864	19.96928906666006	39.20225873961339
290-294	21.156470503736102	38.66079853775606	19.555283668884964	20.62744728962287
295-299	39.09620868253145	20.032659382003093	39.73078345291068	1.1403484825547776
300-301	4.489450086493992	2.5712839944926276	0.5801569836544006	92.35910893535898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.25
34	0.5
35	0.5
36	2.4166666666666665
37	2.0
38	2.333333333333333
39	3.0
40	1.583333333333333
41	0.8333333333333333
42	9.583333333333334
43	156.75000000000009
44	170.5833333333338
45	26.666666666666643
46	6.249999999999999
47	8.75
48	18.749999999999996
49	36.5
50	87.99999999999997
51	1367.8333333333323
52	16541.583333333358
53	6596.999999999998
54	562.4166666666666
55	319.83333333333337
56	266.0833333333333
57	170.08333333333331
58	41.58333333333333
59	12.083333333333334
60	9.083333333333334
61	6.833333333333333
62	6.0
63	7.5
64	4.75
65	3.25
66	6.0
67	4.0
68	3.25
69	1.0
70	3.333333333333333
71	1.25
72	0.25
73	0.75
74	2.5
75	2.3333333333333335
76	0.5
77	1.25
78	2.083333333333333
79	0.75
80	0.75
81	0.75
82	1.25
83	0.5
84	0.25
85	1.5
86	0.5
87	0.5
88	0.3333333333333333
89	0.8333333333333333
90	0.5833333333333333
91	0.25
92	0.0
93	0.0
94	0.75
95	0.5
96	0.25
97	0.0
98	1.0
99	5.5
100	7.33333333333333
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.6576699269530767
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	1.907898810039748
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
40-49	1.0
50-59	0.0
60-69	1.0
70-79	1.0
80-89	4.0
90-99	0.0
100-109	1.0
110-119	5.0
120-129	1.0
130-139	0.0
140-149	3.0
150-159	2.0
160-169	0.0
170-179	0.0
180-189	10.0
190-199	8.0
200-209	6.0
210-219	12.0
220-229	1.0
230-239	0.0
240-249	5.0
250-259	1.0
260-269	1.0
270-279	2.0
280-289	1.0
290-299	935.0
300-302	79818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	5.482621660748092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.8939291356353	3.8320196983382617
2	8.32769126607989	0.9131516103886463
3	3.7689009252990298	0.6199037355077396
4	2.7533288196795307	0.6038184090374787
5	2.0311442112389977	0.5567997624321014
6	1.7603249830737984	0.5790717529293854
7	1.5346422929361316	0.5889704153726227
8	1.3540961408259988	0.5939197465942414
9	0.7447528774542992	0.3674878432051869
>10	6.5899345520198604	7.181479602568702
>50	0.6770480704129994	2.6516042019822073
>100	0.3610923042202663	2.478377609225553
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.2031144211238998	79.03339561241786
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	8288	10.255014291193902	No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7886	9.757606503421226	No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	7774	9.619025229215902	No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6975	8.63039631769757	No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6890	8.525223029238175	No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6856	8.483153713854415	No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6786	8.396540417476087	No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6368	7.879335304816937	No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGAGCCTCAG	6051	7.4871008055036565	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	163	0.20168524728096118	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	162	0.20044791447555646	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	151	0.18683725361610512	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	136	0.16827726153503508	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	134	0.16580259592422575	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	133	0.16456526311882108	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	128	0.15837859909179772	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	121	0.14971726945396505	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	121	0.14971726945396505	No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	117	0.14476793823234635	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	109	0.134869275789109	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	108	0.13363194298370432	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	107	0.13239461017829968	No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	107	0.13239461017829968	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	104	0.12868261176208565	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	102	0.1262079461512763	No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG	99	0.12249594773506231	No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	98	0.12125861492965763	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	94	0.11630928370803895	No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	93	0.11507195090263429	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	89	0.11012261968101561	No Hit
GACTACTGGGGTATCTAATCCCAGTTTGGGTCCTAGCTTTCGTGGGTTAA	88	0.10888528687561093	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	85	0.10517328845939691	No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	84	0.10393595565399226	No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	84	0.10393595565399226	No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG	82	0.10146129004318291	No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA	81	0.10022395723777823	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
