##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	DAFS2_R1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37520
Sequences flagged as poor quality	0
Sequence length	37-301
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.663965884861405	40.0	40.0	40.0	40.0	40.0
2	39.05069296375267	40.0	40.0	40.0	40.0	40.0
3	39.18518123667378	40.0	40.0	40.0	40.0	40.0
4	39.05319829424307	40.0	40.0	40.0	40.0	40.0
5	39.21396588486141	40.0	40.0	40.0	40.0	40.0
6	39.28912579957356	40.0	40.0	40.0	40.0	40.0
7	39.320149253731344	40.0	40.0	40.0	40.0	40.0
8	39.42388059701493	40.0	40.0	40.0	40.0	40.0
9	38.95511727078891	40.0	40.0	40.0	40.0	40.0
10-14	39.58788912579958	40.0	40.0	40.0	40.0	40.0
15-19	39.48407249466951	40.0	40.0	40.0	40.0	40.0
20-24	39.42763326226013	40.0	40.0	40.0	40.0	40.0
25-29	39.59710021321962	40.0	40.0	40.0	40.0	40.0
30-34	39.49697228144989	40.0	40.0	40.0	40.0	40.0
35-39	39.591423360138606	40.0	40.0	40.0	40.0	40.0
40-44	39.54819691356379	40.0	40.0	40.0	40.0	40.0
45-49	39.50938990911271	40.0	40.0	40.0	40.0	40.0
50-54	39.46751246035342	40.0	40.0	40.0	40.0	40.0
55-59	39.409807210909015	40.0	40.0	40.0	40.0	40.0
60-64	39.446975994618604	40.0	40.0	40.0	40.0	40.0
65-69	39.459231814910574	40.0	40.0	40.0	40.0	40.0
70-74	39.40438734440387	40.0	40.0	40.0	40.0	40.0
75-79	39.408104370387356	40.0	40.0	40.0	40.0	40.0
80-84	39.373016211604096	40.0	40.0	40.0	40.0	40.0
85-89	39.56321253359046	40.0	40.0	40.0	40.0	40.0
90-94	39.43900370676551	40.0	40.0	40.0	40.0	40.0
95-99	39.3495933224886	40.0	40.0	40.0	40.0	40.0
100-104	39.23383556894851	40.0	40.0	40.0	40.0	40.0
105-109	39.34323581962185	40.0	40.0	40.0	40.0	40.0
110-114	39.33124210973759	40.0	40.0	40.0	40.0	40.0
115-119	39.247835711428195	40.0	40.0	40.0	40.0	40.0
120-124	39.19241406451854	40.0	40.0	40.0	40.0	40.0
125-129	39.22609926819347	40.0	40.0	40.0	40.0	40.0
130-134	39.253940735603976	40.0	40.0	40.0	40.0	40.0
135-139	39.057072476780185	40.0	40.0	40.0	40.0	40.0
140-144	39.34497344714971	40.0	40.0	40.0	40.0	40.0
145-149	39.037140189409094	40.0	40.0	40.0	40.0	40.0
150-154	39.12233693477391	40.0	40.0	40.0	40.0	40.0
155-159	39.07175670268107	40.0	40.0	40.0	40.0	40.0
160-164	39.16422728448261	40.0	40.0	40.0	40.0	40.0
165-169	39.16546127195612	40.0	40.0	40.0	40.0	40.0
170-174	38.98290109889603	40.0	40.0	40.0	40.0	40.0
175-179	39.24206931511966	40.0	40.0	40.0	40.0	40.0
180-184	39.16054809780091	40.0	40.0	40.0	40.0	40.0
185-189	39.08284534284875	40.0	40.0	40.0	40.0	40.0
190-194	39.21131420947298	40.0	40.0	40.0	40.0	40.0
195-199	39.1728245496998	40.0	40.0	40.0	40.0	40.0
200-204	39.30327951967979	40.0	40.0	40.0	40.0	40.0
205-209	39.11189624806533	40.0	40.0	40.0	40.0	40.0
210-214	39.208001828036586	40.0	40.0	40.0	40.0	40.0
215-219	39.27087959009394	40.0	40.0	40.0	40.0	40.0
220-224	39.15907344150298	40.0	40.0	40.0	40.0	40.0
225-229	39.20100341588386	40.0	40.0	40.0	40.0	40.0
230-234	39.08744662681469	40.0	40.0	40.0	40.0	40.0
235-239	39.18588812980359	40.0	40.0	40.0	40.0	40.0
240-244	39.163364645602044	40.0	40.0	40.0	40.0	40.0
245-249	39.08450042698548	40.0	40.0	40.0	40.0	40.0
250-254	39.16458155422716	40.0	40.0	40.0	40.0	40.0
255-259	38.95708795900939	40.0	40.0	40.0	40.0	40.0
260-264	38.887105038428686	40.0	40.0	40.0	40.0	40.0
265-269	38.988759265035725	40.0	40.0	40.0	40.0	40.0
270-274	38.2456406287529	40.0	40.0	40.0	36.8	40.0
275-279	38.840658233008256	40.0	40.0	40.0	40.0	40.0
280-284	38.398313317320515	40.0	40.0	40.0	36.8	40.0
285-289	38.9033573525487	40.0	40.0	40.0	40.0	40.0
290-294	38.89762358610722	40.0	40.0	40.0	40.0	40.0
295-299	38.82352519049295	40.0	40.0	40.0	40.0	40.0
300-301	38.40288799974063	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.10113512003271552
1101	2	0.13882917521000593
1101	3	0.17480644422213487
1101	4	-0.11941948646322231
1101	5	0.2309264514094309
1101	6	0.11826325494917711
1101	7	0.34703792611981754
1101	8	0.3115519302688696
1101	9	0.08546649061500489
1101	10-14	0.05793456748128989
1101	15-19	0.2842501602857652
1101	20-24	-0.024674614711713616
1101	25-29	0.2184098255203395
1101	30-34	0.17753559281862152
1101	35-39	0.17313543557587963
1101	40-44	0.057776333771073496
1101	45-49	0.09311150865359963
1101	50-54	0.21403590788578697
1101	55-59	0.31532390388778
1101	60-64	0.14563329738930975
1101	65-69	0.18596725044628215
1101	70-74	-0.030247021246950112
1101	75-79	0.0776739299045559
1101	80-84	-0.06064132682251255
1101	85-89	0.2107978561443673
1101	90-94	-0.08521649575709489
1101	95-99	-0.07617059289471939
1101	100-104	0.25188405324841057
1101	105-109	0.27189254004303365
1101	110-114	0.17601679678176652
1101	115-119	0.10327751748746294
1101	120-124	-0.02515417864326963
1101	125-129	-0.021965827661986737
1101	130-134	0.1753098447514745
1101	135-139	0.2066040608310047
1101	140-144	-0.024574381513552623
1101	145-149	0.1271406877307939
1101	150-154	0.012965386766154552
1101	155-159	0.2475061654079198
1101	160-164	-0.01380775676239665
1101	165-169	0.10993033428161425
1101	170-174	0.22791910752702194
1101	175-179	-0.03983690335824974
1101	180-184	0.010449337236011047
1101	185-189	0.10139687563789579
1101	190-194	0.06393310065837454
1101	195-199	0.2115721658668548
1101	200-204	0.11823627347909138
1101	205-209	0.05124954357916067
1101	210-214	0.08574970009826899
1101	215-219	0.19947945000329526
1101	220-224	-0.008811338268642999
1101	225-229	0.025346424057417494
1101	230-234	-0.036113996098954715
1101	235-239	0.05134887315225711
1101	240-244	0.006974122555995166
1101	245-249	-0.09069030398102029
1101	250-254	0.0320428684287819
1101	255-259	0.14907579101402746
1101	260-264	0.2751388061766562
1101	265-269	-0.014801249472760958
1101	270-274	0.21298724832620053
1101	275-279	0.2232423564920083
1101	280-284	0.3117941391659116
1101	285-289	0.2646798883042507
1101	290-294	0.13942819137994888
1101	295-299	-0.06459045355413906
1101	300-301	0.11855262508759523
1102	1	-0.03424298678907434
1102	2	-0.36778108391214914
1102	3	0.5582014733072711
1102	4	0.2227275256996606
1102	5	-0.04194504515323416
1102	6	0.2393632020671035
1102	7	-0.18336926584211
1102	8	0.04502628246347484
1102	9	-0.15884868653994033
1102	10-14	0.01954218249979789
1102	15-19	0.10392229143330667
1102	20-24	0.2345533070228143
1102	25-29	0.163095177186122
1102	30-34	0.07388672978319022
1102	35-39	0.16298207756423722
1102	40-44	0.12356163255478947
1102	45-49	0.23039337010256844
1102	50-54	0.33376092110630395
1102	55-59	0.11437202657419476
1102	60-64	0.26398337671241734
1102	65-69	0.33752727159910734
1102	70-74	0.22231775929244435
1102	75-79	0.009879112347725538
1102	80-84	0.2565453468950949
1102	85-89	0.128830643029616
1102	90-94	0.16057937944121647
1102	95-99	0.30542644570920174
1102	100-104	0.15183117117543787
1102	105-109	0.20642453369718083
1102	110-114	0.07585815056283707
1102	115-119	0.28307656561015904
1102	120-124	0.12524243690406678
1102	125-129	0.34567034367592697
1102	130-134	0.23855680403227808
1102	135-139	0.19422965575432016
1102	140-144	0.2269327575663027
1102	145-149	0.2649513699095394
1102	150-154	0.287211817226229
1102	155-159	-0.1436096463319032
1102	160-164	0.15604946164057054
1102	165-169	0.2566252047205353
1102	170-174	0.4124775422176583
1102	175-179	0.30907901414571626
1102	180-184	0.2459030523109007
1102	185-189	0.22088501213483624
1102	190-194	0.1861405120153492
1102	195-199	0.30280117146536156
1102	200-204	0.2430030023513865
1102	205-209	0.4077944649334668
1102	210-214	0.13490992403854563
1102	215-219	0.17959142014459673
1102	220-224	0.22840540393342934
1102	225-229	0.21206996444664838
1102	230-234	0.186120074815463
1102	235-239	0.16614492753775778
1102	240-244	0.1393654848593826
1102	245-249	0.09043472934342134
1102	250-254	0.105409970534879
1102	255-259	0.12748154947844625
1102	260-264	0.0906546686133396
1102	265-269	0.350967878759711
1102	270-274	0.632715320840191
1102	275-279	0.32001655004039975
1102	280-284	0.20270323007500224
1102	285-289	0.1190653855049888
1102	290-294	0.11543458967373965
1102	295-299	0.22289563097586296
1102	300-301	0.07805117860737454
1103	1	0.22911833534873693
1103	2	0.2615671767835721
1103	3	0.07226251555703556
1103	4	-0.16111893571577696
1103	5	0.22568123766697568
1103	6	-0.04932132412242396
1103	7	0.08399045693544593
1103	8	0.30945384477188753
1103	9	-0.11699875984395192
1103	10-14	0.12669931964493486
1103	15-19	0.146038778171949
1103	20-24	0.1526660619208613
1103	25-29	0.0348797966716603
1103	30-34	0.20743331115064478
1103	35-39	0.04127076209042713
1103	40-44	-0.0104114448809014
1103	45-49	0.25875535864048516
1103	50-54	0.03601335346669288
1103	55-59	0.24818516798428902
1103	60-64	0.16205081640320884
1103	65-69	0.07901734223752044
1103	70-74	0.25110624389859737
1103	75-79	0.20340170331133578
1103	80-84	0.13568702355776452
1103	85-89	0.15063525451834892
1103	90-94	0.1895278000729661
1103	95-99	0.3081986701527626
1103	100-104	0.28681717677319796
1103	105-109	0.22966856941622638
1103	110-114	0.48305527171437035
1103	115-119	0.46012574593802924
1103	120-124	0.5908162073958678
1103	125-129	0.36534247482347126
1103	130-134	0.5139666400978626
1103	135-139	0.43029522952480903
1103	140-144	0.2334901346154865
1103	145-149	0.4354431731882258
1103	150-154	0.3199987024721338
1103	155-159	0.4137674028484213
1103	160-164	0.2544101173782778
1103	165-169	0.17793668013036523
1103	170-174	0.392805411070114
1103	175-179	0.36809540758834913
1103	180-184	0.08321506965272363
1103	185-189	0.45581252873150646
1103	190-194	0.34145821964304446
1103	195-199	0.4445705794046688
1103	200-204	0.38433886470065914
1103	205-209	0.16240766097736525
1103	210-214	0.3827354219385626
1103	215-219	0.2759469275284232
1103	220-224	0.18738115247698772
1103	225-229	0.49853521305794146
1103	230-234	0.5130134496969205
1103	235-239	0.13818123698091966
1103	240-244	0.18412906702981502
1103	245-249	0.208304936632409
1103	250-254	0.35377740042243744
1103	255-259	0.14791238540853158
1103	260-264	0.2952339939080204
1103	265-269	0.38223735444051243
1103	270-274	0.16920089190102772
1103	275-279	0.2741149106961345
1103	280-284	0.5544170899855843
1103	285-289	0.29736100940419163
1103	290-294	0.1301209465359534
1103	295-299	0.3004368688502339
1103	300-301	0.29210634409586334
1104	1	0.09473682624439306
1104	2	0.5131293618269126
1104	3	-0.10485231344249257
1104	4	-0.1804698730268015
1104	5	0.21493071693862476
1104	6	-0.06222194899643796
1104	7	0.3051824209211986
1104	8	-0.09154057839548813
1104	9	-0.6642002649168504
1104	10-14	-0.06293986600312707
1104	15-19	-0.005006038061338813
1104	20-24	-0.09212329506361527
1104	25-29	-0.07681811369587876
1104	30-34	-0.27343748102834553
1104	35-39	-0.03726179183015432
1104	40-44	0.03495575259271533
1104	45-49	-0.17529691576655893
1104	50-54	-0.09745436137573904
1104	55-59	-0.04148761304104198
1104	60-64	-0.16132484710549022
1104	65-69	0.13088860475180297
1104	70-74	-0.197029212662585
1104	75-79	0.05117432979792369
1104	80-84	-0.13630115086943562
1104	85-89	-0.04540049096306831
1104	90-94	-0.034136783680310145
1104	95-99	-0.18664779897309813
1104	100-104	0.10717597540249812
1104	105-109	-0.1676169240998675
1104	110-114	-0.015132230144395464
1104	115-119	-0.19008425270714469
1104	120-124	-0.4092650824022641
1104	125-129	-0.16678054373770834
1104	130-134	-0.3934984730304478
1104	135-139	-0.16913574188826885
1104	140-144	-0.27229498935146523
1104	145-149	-0.2561704293046603
1104	150-154	-0.021851995265308233
1104	155-159	-0.2580656820994136
1104	160-164	-0.0544369223182386
1104	165-169	-0.19713444844298778
1104	170-174	-0.015269375544164632
1104	175-179	-0.2559392760588608
1104	180-184	-0.07454133191888701
1104	185-189	-0.1890323432370664
1104	190-194	0.12663637978394604
1104	195-199	-0.1533971756420769
1104	200-204	0.024821184169574906
1104	205-209	-0.4517096672979406
1104	210-214	-0.05021404290360465
1104	215-219	-0.19065651373969672
1104	220-224	-0.06911525722359357
1104	225-229	0.07983855948796759
1104	230-234	0.027442388865047462
1104	235-239	0.10664079530634751
1104	240-244	-0.27055187051252005
1104	245-249	-0.08807766735078815
1104	250-254	0.026071127559674778
1104	255-259	-0.05103084721577744
1104	260-264	-0.24653541403129253
1104	265-269	-0.072172617108059
1104	270-274	-0.021830133705265098
1104	275-279	-0.42709088797613504
1104	280-284	-0.18737941455309937
1104	285-289	-0.10572800292476359
1104	290-294	-0.28125962520229564
1104	295-299	-0.05850755598416413
1104	300-301	-0.7371325607922827
1201	1	-0.11699084002872695
1201	2	-0.2595323199256825
1201	3	-0.19641731409839736
1201	4	0.025485787474316624
1201	5	-0.7747059090616375
1201	6	0.3502140998032459
1201	7	-0.6632027292821334
1201	8	-0.2557803789989208
1201	9	-0.3763870680127468
1201	10-14	0.13320338468558646
1201	15-19	0.2048850809206897
1201	20-24	0.04178723884691493
1201	25-29	-0.03914265783859605
1201	30-34	0.20170353956721954
1201	35-39	0.10042678222205836
1201	40-44	0.17820644129795937
1201	45-49	0.08082272217125563
1201	50-54	0.1870005776246515
1201	55-59	0.027356673985067914
1201	60-64	0.17707210947327923
1201	65-69	0.16620278552052525
1201	70-74	0.3156823182307704
1201	75-79	0.14966573738023925
1201	80-84	0.31160649703821264
1201	85-89	0.21397245931897402
1201	90-94	0.013298609209627443
1201	95-99	0.12577513163165577
1201	100-104	0.1519352565254195
1201	105-109	0.1257583874572603
1201	110-114	-0.057162926720543794
1201	115-119	0.24664669311470533
1201	120-124	0.21652528884264655
1201	125-129	0.015719784217168353
1201	130-134	0.06327707465419508
1201	135-139	-0.02268421361856099
1201	140-144	0.20174410286944777
1201	145-149	0.06354621768471702
1201	150-154	-0.03670179193328238
1201	155-159	0.44207861804488857
1201	160-164	0.10744160319664076
1201	165-169	0.17741625338043576
1201	170-174	0.13175935330274058
1201	175-179	0.054069906677597146
1201	180-184	0.08602537410236266
1201	185-189	0.2344229893091807
1201	190-194	-0.10319439550138299
1201	195-199	0.2970816384731023
1201	200-204	-0.044180521195769984
1201	205-209	0.21663612832058732
1201	210-214	0.28708098530051984
1201	215-219	0.2714712574789786
1201	220-224	0.29646259926335716
1201	225-229	0.29098902518389025
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2404	245-249	-0.051433879705463426
2404	250-254	-0.298848328946832
2404	255-259	-0.010464941626757707
2404	260-264	0.18831540541120972
2404	265-269	-0.08500354580954905
2404	270-274	-0.2550280605857722
2404	275-279	0.07594875343022522
2404	280-284	-0.2915956913425717
2404	285-289	0.06096200968204357
2404	290-294	0.008865042118053168
2404	295-299	0.27670663039368293
2404	300-301	0.5929260940908065
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	5.0
20	9.0
21	18.0
22	44.0
23	44.0
24	86.0
25	79.0
26	101.0
27	82.0
28	88.0
29	114.0
30	110.0
31	156.0
32	163.0
33	212.0
34	259.0
35	350.0
36	504.0
37	764.0
38	1568.0
39	29648.0
40	3110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.07196162046908315	0.010660980810234541	0.05863539445628998	99.85874200426439
2	0.007995948719315547	0.021322529918174793	0.15991897438631095	99.81076254697619
3	0.005330490405117271	0.1359275053304904	99.60554371002132	0.2531982942430704
4	0.09594882729211088	99.41897654584221	0.10927505330490404	0.3757995735607676
5	0.16257995735607675	0.12260127931769724	0.10927505330490404	99.60554371002132
6	99.7681236673774	0.0826226012793177	0.015991471215351813	0.13326226012793177
7	99.86140724946695	0.0826226012793177	0.042643923240938165	0.013326226012793176
8	99.7681236673774	0.1199360341151386	0.0746268656716418	0.0373134328358209
9	29.291044776119403	23.294243070362473	25.642324093816633	21.77238805970149
10-14	59.84381663113007	9.899786780383796	10.09861407249467	20.15778251599147
15-19	37.98400852878465	21.733475479744136	19.959488272921106	20.323027718550108
20-24	59.80863539445629	39.67484008528785	0.43230277185501065	0.08422174840085288
25-29	17.420042643923242	17.64818763326226	60.3544776119403	4.5772921108742
30-34	5.075692963752665	56.11300639658848	1.021321961620469	37.78997867803838
35-39	64.2682757811917	2.565592383714112	19.904796426401134	13.261335408693057
40-44	33.748234227991155	49.49279031957142	0.39553292998214234	16.36344252245529
45-49	20.087955435912473	19.92163970255071	22.29483728244356	37.69556757909326
50-54	40.4275167248594	20.78946667022042	16.43913750366481	22.343879101255364
55-59	25.342630055492478	25.949795568064904	10.077668143267605	38.62990623317501
60-64	39.876110157474436	0.5170961585619396	0.7495228855031826	58.85727079846043
65-69	33.689793960071434	23.114854599248343	39.330969960284676	3.864381480395554
70-74	40.35770450729002	56.87608284244475	2.581229842471413	0.18498280779380016
75-79	37.44468023844817	22.31132628795069	13.250615848911734	26.99337762468941
80-84	39.225682593856654	1.9133959044368603	38.23485494880546	20.626066552901023
85-89	31.787129425693152	8.038376005930257	55.05698271586503	5.1175118525115595
90-94	14.614789727726071	59.08370889890398	14.261180298141285	12.040321075228674
95-99	6.699911997653271	11.706978852769408	59.59358916237766	21.99951998719966
100-104	28.369289847729274	22.22405930824822	17.423397957278862	31.983252886743646
105-109	64.09877596735913	31.912317661804313	1.6165764420384543	2.3723299287981012
110-114	41.208536240619146	52.63143858376493	3.373639209955036	2.7863859656608865
115-119	38.52353647152953	34.782237631684225	19.288971862915055	7.4052540338711825
120-124	25.14508833319109	29.891930528292228	29.97300930272254	14.989971835794144
125-129	12.844462462526538	42.848303158972826	23.181803633725583	21.125430744775052
130-134	24.145307123996478	33.0317659296402	30.18803509988531	12.634891846478007
135-139	12.620623803097144	20.16771309538416	26.338530803411874	40.87313229810683
140-144	38.877062693064865	18.228524784327536	19.604987275724646	23.289425246882953
145-149	3.4499132986527945	31.930105375483524	31.111644657863145	33.50833666800053
150-154	34.4484460450847	14.324129651860746	15.517140189409098	35.71028411364546
155-159	28.927304255035345	56.279578498065895	0.7640389489128985	14.029078297985862
160-164	29.054976950657334	13.261908827044563	1.6235914290592453	56.059522793238855
165-169	33.14053379149903	27.908267258582605	24.58300874000875	14.368190209909612
170-174	9.794672586015539	31.852962520276616	19.96606334841629	38.38630154529156
175-179	53.849150236119634	7.036631893492704	16.399242282756596	22.714975587631066
180-184	34.35809044046234	35.929111924608044	1.665474881799844	28.047322753129773
185-189	34.64582477225303	6.997048762134497	25.002801808100163	33.354324657512315
190-194	27.68138759172782	29.526350900600402	23.994663108739157	18.79759839893262
195-199	23.494062708472317	34.10060040026684	24.711407605070047	17.693929286190794
200-204	65.91701134089392	3.4337558372248167	25.794262841894593	4.854969979986658
205-209	24.05667929764637	42.464108448524314	12.448631050861932	21.03058120296739
210-214	44.797826690292695	28.340556350206015	23.981661365043443	2.8799555944578468
215-219	39.14656276686593	18.296861656703673	19.33763877028181	23.21893680614859
220-224	28.372651579846288	26.271349274124677	27.70388556789069	17.652113578138344
225-229	44.93061485909479	10.718403074295473	23.965627668659266	20.38535439795047
230-234	22.0415243381725	48.86208368915457	21.67271562766866	7.423676345004269
235-239	46.743168232280105	22.605678906917166	8.869555935098207	21.781596925704527
240-244	33.0812339880444	33.72918445772844	12.044726729291204	21.14485482493595
245-249	40.716268146883	13.032664389410762	19.963172502134928	26.287894961571308
250-254	34.293872758326216	6.467762596071734	45.285546541417595	13.952818104184459
255-259	12.625426985482493	38.88770281810419	15.244449188727582	33.24242100768574
260-264	37.17175491033305	28.044940222032448	20.122758326216907	14.66054654141759
265-269	22.19761099072364	42.31631422197077	20.037041386009673	15.449033401295914
270-274	14.819994128792931	7.460702943609725	31.69864695364415	46.02065597395319
275-279	33.79752443274923	27.212558246286385	18.28119412226249	20.7087231987019
280-284	13.156658660261542	31.989324793167867	18.813450760608486	36.0405657859621
285-289	41.23352014945289	17.170002668801708	28.771283693621562	12.825193488123832
290-294	31.35658811283995	22.62296832047826	22.838613253622995	23.181830313058796
295-299	34.02258057598696	23.862929684987026	25.599360967555164	16.515128771470845
300-301	31.39200304025625	47.25426857406553	21.2682211786422	0.0855072070360216
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.6666666666666666
41	0.0
42	0.25
43	0.25
44	0.3333333333333333
45	17.250000000000007
46	52.50000000000009
47	77.33333333333334
48	60.833333333333336
49	136.25
50	517.8333333333333
51	423.75
52	340.50000000000006
53	1245.2499999999993
54	3686.166666666673
55	4834.833333333335
56	1252.8333333333337
57	225.4166666666667
58	50.583333333333336
59	20.083333333333332
60	4.666666666666667
61	4.416666666666667
62	3.75
63	6.083333333333333
64	4.333333333333334
65	2.833333333333333
66	1.0
67	1.0
68	0.75
69	1.0
70	2.0
71	1.5
72	0.25
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0026652452025586353
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	0.0
290-294	5.337717378540241E-4
295-299	0.0
300-301	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
30-39	1.0
40-49	0.0
50-59	1.0
60-69	1.0
70-79	13.0
80-89	5.0
90-99	0.0
100-109	0.0
110-119	4.0
120-129	2.0
130-139	2.0
140-149	6.0
150-159	0.0
160-169	3.0
170-179	1.0
180-189	6.0
190-199	0.0
200-209	1.0
210-219	2.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	1.0
270-279	1.0
280-289	0.0
290-299	542.0
300-302	36928.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.69136460554371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.28150873965041	5.413113006396589
2	14.627414903403862	2.5426439232409384
3	6.439742410303588	1.6791044776119404
4	3.526525605642441	1.2260127931769722
5	1.9625881631401412	0.8528784648187633
6	1.4719411223551058	0.767590618336887
7	0.7053051211284882	0.4291044776119403
8	0.8892977614228765	0.6183368869936035
9	0.7973014412756823	0.6236673773987207
>10	5.151793928242871	8.102345415778252
>50	0.6439742410303588	4.386993603411514
>100	1.011959521619135	15.980810234541579
>500	0.18399264029438822	14.200426439232409
>1k	0.306654400490647	43.17697228144989
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	2183	5.818230277185501	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1879	5.007995735607676	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1869	4.981343283582089	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1792	4.776119402985075	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1722	4.58955223880597	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1637	4.363006396588486	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1540	4.104477611940299	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT	1411	3.760660980810235	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1112	2.963752665245203	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	1055	2.8118336886993602	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	990	2.638592750533049	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	955	2.545309168443497	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	920	2.4520255863539444	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	878	2.340085287846482	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	834	2.222814498933902	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT	751	2.001599147121535	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	355	0.9461620469083156	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	313	0.8342217484008528	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	307	0.8182302771855011	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	302	0.8049040511727079	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	301	0.8022388059701492	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	272	0.7249466950959489	No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	238	0.6343283582089552	No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	231	0.6156716417910448	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	225	0.599680170575693	No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	225	0.599680170575693	No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	208	0.5543710021321961	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT	200	0.5330490405117271	No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	174	0.4637526652452026	No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	170	0.453091684434968	No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	170	0.453091684434968	No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	168	0.44776119402985076	No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGAT	164	0.4371002132196162	No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	152	0.4051172707889125	No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	147	0.39179104477611937	No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	138	0.3678038379530917	No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	137	0.36513859275053306	No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	132	0.35181236673773986	No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	130	0.3464818763326226	No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	128	0.3411513859275053	No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	121	0.3224946695095949	No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	118	0.31449893390191896	No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	116	0.3091684434968017	No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	115	0.30650319829424305	No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	113	0.3011727078891258	No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	111	0.2958422174840085	No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	109	0.2905117270788913	No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	103	0.27452025586353945	No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC	103	0.27452025586353945	No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	100	0.26652452025586354	No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	100	0.26652452025586354	No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	100	0.26652452025586354	No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	99	0.26385927505330486	No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC	94	0.2505330490405117	No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC	92	0.24520255863539447	No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	91	0.2425373134328358	No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC	80	0.21321961620469082	No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC	79	0.21055437100213217	No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT	76	0.20255863539445626	No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	76	0.20255863539445626	No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC	76	0.20255863539445626	No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC	74	0.19722814498933902	No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC	73	0.19456289978678037	No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC	70	0.18656716417910446	No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	65	0.1732409381663113	No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	65	0.1732409381663113	No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	61	0.16257995735607675	No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	59	0.15724946695095948	No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	59	0.15724946695095948	No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	57	0.15191897654584222	No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC	50	0.13326226012793177	No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA	42	0.11194029850746269	No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA	40	0.10660980810234541	No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC	38	0.10127931769722813	No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGATGGCCTGAA	38	0.10127931769722813	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-14	0.0	0.0	0.0	0.0	0.0
15-19	0.0	0.0	0.0	0.0	0.0
20-24	0.0	0.0	0.0	0.0	0.0
25-29	0.0	0.0	0.0	0.0	0.0
30-34	0.0	0.0	0.0	0.0	0.0
35-39	0.0	0.0	0.0	0.0	0.0
40-44	0.0	0.0	0.0	0.0	0.0
45-49	0.0	0.0	0.0	0.0	0.0
50-54	0.0	0.0	0.0	0.0	0.0
55-59	0.0	0.0	0.0	0.0	0.0
60-64	0.0	0.0	0.0	0.0	0.0
65-69	0.0	0.0	0.0	0.0	0.0
70-74	0.0	0.0	0.0	0.0	0.0
75-79	0.0	0.0	0.0	0.0	0.0
80-84	0.0	0.0	0.0	0.0	0.0
85-89	0.0	0.0	0.0	0.0	0.0
90-94	0.0	0.0	0.0	0.0	0.0
95-99	0.0	0.0	0.0	0.0	0.0
100-104	0.0	0.0	0.0	0.0	0.0
105-109	0.0	0.0	0.0	0.0	0.0
110-114	0.0	0.0	0.0	0.0	0.0
115-119	0.0	0.0	0.0	0.0	0.0
120-124	0.0	0.0	0.0	0.0	0.0
125-129	0.0	0.0	0.0	0.0	0.0
130-134	0.0	0.0	0.0	0.0	0.0
135-139	0.0	0.0	0.0	0.0	0.0
140-144	0.0	0.0	0.0	0.0	0.0
145-149	0.0	0.0	0.0	0.0	0.0
150-154	0.0	0.0	0.0	0.0	0.0
155-159	0.0	0.0	0.0	0.0	0.0
160-164	0.0	0.0	0.0	0.0	0.0
165-169	0.0	0.0	0.0	0.0	0.0
170-174	0.0	0.0	0.0	0.0	0.0
175-179	0.0	0.0	0.0	0.0	0.0
180-184	0.0	0.0	0.0	0.0	0.0
185-189	0.0	0.0	0.0	0.0	0.0
190-194	0.0	0.0	0.0	0.0	0.0
195-199	0.0	0.0	0.0	0.0	0.0
200-204	0.0	0.0	0.0	0.0	0.0
205-209	0.0	0.0	0.0	0.0	0.0
210-214	0.0	0.0	0.0	0.0	0.0
215-219	0.0	0.0	0.0	0.0	0.0
220-224	0.0	0.0	0.0	0.0	0.0
225-229	0.0	0.0	0.0	0.0	0.0
230-234	0.0	0.0	0.0	0.0	0.0
235-239	0.0	0.0	0.0	0.0	0.0
240-244	0.0	0.0	0.0	0.0	0.0
245-249	0.0	0.0	0.0	0.0	0.0
250-254	0.0	0.0	0.0	0.0	0.0
255-259	0.0	0.0	0.0	0.0	0.0
260-264	0.0	0.0	0.0	0.0	0.0
265-269	0.0	0.0	0.0	0.0	0.0
270-274	0.0	0.0	0.0	0.0	0.0
275-279	0.0	0.0	0.0	0.0	0.0
280-284	0.0	0.0	0.0	0.0	0.0
285-289	0.0	0.0	0.0	0.0	0.0
290	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
