FastQCFastQC Report
Mon 14 Oct 2024
CVMS2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCVMS2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228355
Sequences flagged as poor quality0
Sequence length51-301
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT50062.1922007400757595No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC49112.1505988482844693No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT48862.139650982023604No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC48432.1208206520549147No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT47022.0590746863436316No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT45561.9951391473801756No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC45171.978060476013225No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT45101.9749950734601827No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC44801.9618576339471436No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC44591.9526614262880164No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT44531.9500339383854086No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC43241.8935429484793413No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC42981.882157167568041No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC42861.8769021917628255No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT42821.875150533161087No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC42761.8725230452584791No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC39801.7429003087298285No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC39181.7157496004028814No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT39131.713560027150708No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC38731.6960434411333232No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC38461.6842197455715882No Hit
CCTACGGGCGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC38131.6697685621072453No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC37721.6518140614394254No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC33681.474896542663835No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC33611.4718311401107924No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC32991.4446804317838453No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC31321.3715486851612622No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC30721.3452738061351843No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC30411.3316984519717106No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC30031.3150576952551947No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC28681.2559392174465196No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC25991.1381401764796042No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT13350.5846160583302314No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT13200.5780473385737119No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC12980.5684132162641502No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT12880.5640340697598039No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC12680.5552757767511112No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC12610.5522103741980688No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT12390.5425762518885069No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC12260.5368833614328568No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC12220.5351317028311182No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT12110.5303146416763372No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT12080.5290008977250334No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC12000.5254975805215564No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC11740.514111799610256No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT11600.5079809945041712No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10960.4799544568763548No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT10940.4790786275754856No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT10400.4554312364520155No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT10300.45105208994766927No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT9780.4282805281250684No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT9760.4274046988241992No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT9650.42258763766941826No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT9430.41295351535985636No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT8920.39061986818769023No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT8470.37091370891813186No Hit
CCTACGGGCGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC6340.2776378883755556No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC6270.2745724858225132No Hit
CCTACGGGGGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC6100.2671279367651245No Hit
CCTACGGGTGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC5590.24479428959295835No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5560.24348054564165442No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC5540.24260471634078518No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5430.23778765518600425No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5350.2342843379825272No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5340.23384642333209257No Hit
CCTACGGGAGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC5240.22946727682774626No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC5230.22902936217731165No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC5040.22070898381905368No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4930.2158919226642727No Hit
CCTACGGGCGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC4810.21063694685905715No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4560.19968908059819143No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC4530.19837533664688753No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4240.18567581178428325No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC4040.17691751877559064No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3940.17253837227124433No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3930.1721004576208097No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3900.17078671366950582No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3840.16815922576689804No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3780.16553173786429026No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3780.16553173786429026No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3760.164655908563421No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3590.1572113595060323No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3530.15458387160342452No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3480.15239429835125134No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3470.1519563837008167No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3450.15108055439994744No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3440.1506426397495128No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3420.14976681044864354No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3410.14932889579820893No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC3390.14845306649733966No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3350.14670140789560115No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3300.14451183464342798No Hit
CCTACGGGGGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC3280.14363600534255874No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3180.13925685883821243No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3010.13181230978082373No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2990.13093648047995446No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2940.12874690722778132No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2780.12174027282082721No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT2660.11648529701561165No Hit
CCTACGGGGGGCTGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA2640.1156094677147424No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2570.11254406516169999No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2560.11210615051126535No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2540.1112303212103961No Hit
CCTACGGGTGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2520.11035449190952684No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2480.10860283330778832No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT2470.10816491865735368No Hit
CCTACGGGGGGCAGCAGTGAGGGATATTGGTCAATGGGGGAAACCCTGAA2420.10597534540518054No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2410.1055374307547459No Hit
CCTACGGGAGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2350.10290994285213813No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGCGAAAGCCTGAT2290.10028245494953035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)