FastQCFastQC Report
Mon 14 Oct 2024
CVFS2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCVFS2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206738
Sequences flagged as poor quality0
Sequence length42-301
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT64503.119890876374929No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT64413.115537540268359No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT62773.0362100823264226No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT61132.9568826243844866No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT59902.8973870309280345No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT59052.856272189921543No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT52382.5336416140235465No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT49832.4102970910040726No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC34471.6673277288161827No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC32931.5928373109926575No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC32051.5502713579506429No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC31991.5473691338795965No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC31181.5081891089204693No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC29341.4191875707417116No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC28531.3800075457825847No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC28101.3592082732734185No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC27701.3398601127997756No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC27561.3330882566340005No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC27421.3263164004682255No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC26831.2977778637696022No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC26281.271174143118343No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC24711.1952326132592945No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC23811.1516992521935978No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC21591.0443169615648793No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT13240.6404241116775822No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT13210.638972999642059No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT12830.6205922471920983No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC12760.6172063191092106No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT12690.6138203910263232No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC12200.5901188944461105No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC12090.5847981503158587No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC11860.573672958043514No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC11700.5659336938540568No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT11660.5639988778066926No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC11630.5625477657711693No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT11580.5601292457119639No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC11210.5422321972738442No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC11190.5412647892501621No Hit
CCTACGGGGGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC11020.5330418210488638No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT10860.5253025568594065No Hit
CCTACGGGTGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC10710.5180469966817904No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC10550.5103077324923333No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT10330.49966624423182965No Hit
CCTACGGGCGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC10170.4919269800423725No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10010.4841877158529153No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9930.48031808375818663No Hit
CCTACGGGCGGCAGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC9820.4749973396279349No Hit
CCTACGGGCGGCAGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC9760.47209511555688843No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTCGGCAATGGGGGGAACCCTGAC9650.4667743714266366No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9480.4585514032253384No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9440.4566165871779741No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9200.44500769089378833No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC8990.43484990664512574No Hit
CCTACGGGAGGCTGCAGTTAGGAATATTGGGCAATGGAGGGAACTCTGAC8480.4101810020412309No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC8310.40195803383993267No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC8110.3922839536031112No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC5990.2897387030928034No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC5760.27861351082045877No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5520.267004614536273No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5500.2660372065125908No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC5440.2631349824415444No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC5390.260716462382339No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC5380.2602327583704979No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5270.2549120142402461No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC5220.2524934941810407No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5100.24668904603894784No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC5030.24330311795606033No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4860.23508014975476207No Hit
CCTACGGGTGGCTGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC4750.22975940562451025No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4640.22443866149425845No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT4580.22153643742321197No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC4540.21960162137584768No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT4440.21476458125743694No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT4440.21476458125743694No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT4360.21089494916270835No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC4280.20702531706797977No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC4250.20557420503245655No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC4150.2007371649140458No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT4150.2007371649140458No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT4150.2007371649140458No Hit
CCTACGGGGGGCAGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC4140.2002534609022047No Hit
CCTACGGGGGGCTGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC4130.19976975689036366No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC4130.19976975689036366No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT4110.19880234886668152No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT4110.19880234886668152No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGAAAGCCTTAC4050.19590012479563504No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC4040.195416420783794No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT4030.1949327167719529No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT3960.19154678868906538No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT3950.19106308467722433No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3920.1896119726417011No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC3920.1896119726417011No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3920.1896119726417011No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC3910.18912826862986No Hit
CCTACGGGGGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC3900.18864456461801896No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3880.18767715659433679No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3860.18670974857065464No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3850.18622604455881359No Hit
CCTACGGGTGGCAGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC3830.18525863653513142No Hit
CCTACGGGAGGCAGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC3810.18429122851144927No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3760.1818727084522439No Hit
CCTACGGGCGGCTGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC3760.1818727084522439No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3700.17897048438119745No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3700.17897048438119745No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3690.1784867803693564No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3690.1784867803693564No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3660.17703566833383316No Hit
CCTACGGGTGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC3660.17703566833383316No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3650.17655196432199208No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3620.17510085228646888No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3620.17510085228646888No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC3590.17364974025094565No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3580.17316603623910456No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT3570.1726823322272635No Hit
CCTACGGGCGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC3560.17219862821542242No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3550.17171492420358136No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3500.16929640414437597No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGCGCAAGCCTGAT3470.16784529210885274No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGCAAGCCTGAT3470.16784529210885274No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT3450.1668778840851706No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT3440.16639418007332954No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGCGCAAGCCTGAT3380.16349195600228308No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3360.16252454797860094No Hit
CCTACGGGCGGCAGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC3340.1615571399549188No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTCCGCAATGGGCGAAAGCCTGAC3320.16058973193123663No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGCGCAAGCCTGAT3290.15913861989571343No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGCGCAAGCCTGAT3240.15672009983650803No Hit
CCTACGGGCGGCAGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC3220.1557526918128259No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3150.15236676372993838No Hit
CCTACGGGAGGCTGCAGTAGGGAATTTTGCGCAATGGGCGAAAGCCTGAC3120.15091565169441515No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGCGCAAGCCTGAT3070.14849713163520978No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGAC3050.14752972361152764No Hit
CCTACGGGAGGCTGCAGTTAGGAATATTGGGCAATGGAGGAAACTCTGAC2910.14075786744575258No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2840.13737193936286507No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGCGCAAGCCTGAT2820.13640453133918293No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGCGCAAGCCTGAT2690.13011637918524896No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2520.12189341098395069No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2500.12092600296026855No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2330.11270303475897028No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2290.11076821871160598No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2250.1088334026642417No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC2250.1088334026642417No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2240.10834969865240061No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2210.1068985866168774No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC2190.10593117859319526No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2160.10448006655767203No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2150.10399636254583096No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2140.10351265853398989No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC2110.10206154649846666No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT2080.10061043446294345No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC2070.10012673045110235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)