FastQCFastQC Report
Mon 14 Oct 2024
CPFS2_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCPFS2_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179192
Sequences flagged as poor quality0
Sequence length35-301
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG36422.0324568061074153No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG36042.011250502254565No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG35862.001205410955846No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG31761.7724005535961425No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG31401.7523103709987053No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG31271.7450555828385197No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG28941.615027456582883No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG28581.594937273985446No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG28051.5653600607169964No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG27681.5447118174918524No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG27421.5302022411714808No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG26671.4883476940934863No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA24451.3644582347426224No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG24191.349948658422251No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG23981.3382293852404126No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG23831.3298584758248138No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA23701.3226036876646279No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA23121.2902361712576453No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG22091.2327559266038661No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG21851.219362471538908No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG21501.1998303495691773No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA20831.1624402875128355No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA19921.1116567703915354No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA19811.105518103486763No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA19221.0725925264520737No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA18851.0519442832269297No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17450.9738157953480067No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA16980.9475869458457967No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16480.919683914460467No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG16400.9152194294388143No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14610.8153265770793339No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14260.7957944551096031No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13590.7584043930532613No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13360.7455689986160096No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12950.7226885128800393No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12780.7132014822090271No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG10840.6049377204339479No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG10710.5976829322737622No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG10140.5658734764944864No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG9270.5173222018840127No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8920.4977900799142819No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8740.4877449886155632No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8570.4782579579445511No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG8480.4732354122951917No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGAGCCTCAG7830.4369614714942631No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7210.4023617125764543No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7090.3956649850439752No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG7080.39510692441626855No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA7050.39343274253314886No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG6950.3878521362560829No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG6820.3805973480958971No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6580.36720389303093887No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6490.36218134738157953No Hit
GACTACTGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG6370.3554846198491004No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6360.3549265592213938No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC6160.3437653466672619No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA6030.3365105585070762No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5980.3337202553685432No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG5980.3337202553685432No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG5960.33260413411313006No Hit
GACTACTAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG5930.33092995223001026No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5930.33092995223001026No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA5920.3303718916023037No Hit
GACTACTCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG5900.32925577034689046No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG5880.3281396490914773No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA5840.3259074065806509No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG5680.3169784365373454No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5610.31307201214339925No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5610.31307201214339925No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA5590.3119558908879861No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5530.3086075271217465No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5490.3063752846109201No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG5470.30525916335550696No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG5430.3030269208446806No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG5420.30246886021697394No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG5370.299678557078441No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA5320.29688825393990803No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC5240.2924237689182553No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG5190.28963346577972227No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACACTTTCGTGCCTCAG5190.28963346577972227No Hit
GACTACAGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG5060.28237867761953656No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4970.2773561319701772No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4940.2756819500870574No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4860.27121746506540473No Hit
GACTACAAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG4860.27121746506540473No Hit
GACTACCAGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG4850.2706594044376981No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG4830.26954328318228493No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4820.26898522255457835No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGCCTCAA4740.26452073753292554No Hit
GACTACACGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG4740.26452073753292554No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG4720.2634046162775124No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4690.26173043439439264No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4670.2606143131389794No Hit
GACTACCGGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG4670.2606143131389794No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4600.2567078887450333No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4570.2550337068619135No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4550.2539175856065003No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4490.2505692218402607No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4300.23996606991383546No Hit
GACTACCCGGGTATCTAATCCTATTTGCTCCCCACGCTTTCGTGCCTCAG4270.23829188803071566No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4200.2343854636367695No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG4180.2332693423813563No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG4170.23271128175364975No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG4170.23271128175364975No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG4110.22936291798741015No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG3990.222666190454931No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG3980.22210812982722444No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3960.22099200857181125No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG3960.22099200857181125No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3930.21931782668869146No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3920.21875976606098488No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3920.21875976606098488No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCCGGCTTTCGTTCCTCAG3910.21820170543327827No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3800.21206303852850575No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3770.21038885664538595No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3740.20871467476226618No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3740.20871467476226618No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3730.20815661413455958No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3680.2053663109960266No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3610.20145988660208045No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3610.20145988660208045No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3580.19978570471896068No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3550.19811152283584088No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3480.19420509844189474No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3460.19308897718648155No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3460.19308897718648155No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG3420.19085673467565514No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3330.1858341890262958No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3250.18136970400464306No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3250.18136970400464306No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3230.18025358274922987No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3220.1796955221215233No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA3120.17411491584445735No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGAGCCTCAG3010.16797624893968482No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAA2670.14900218759766062No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2510.14007321755435512No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2450.13672485378811552No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2430.13560873253270236No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2210.12333139872315728No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2180.1216572168400375No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2130.11886691370150454No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG2120.11830885307379793No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2070.11551854993526497No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2070.11551854993526497No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG1920.10714764051966605No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG1900.10603151926425287No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG1900.10603151926425287No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG1890.10547345863654628No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGTGCCTCAG1870.10435733738113309No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGTGTCTCAG1860.10379927675342648No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1840.1026831554980133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)