FastQCFastQC Report
Mon 14 Oct 2024
CAMS1_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCAMS1_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146978
Sequences flagged as poor quality0
Sequence length100-301
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT78735.356583978554614No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT75835.159275537835595No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT73134.975574575786852No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT70544.799357727006763No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT69894.755133421328362No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT68434.655798827035339No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT66824.546258623739607No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT59204.027813686402046No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT20031.36278898882826No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT19931.3559852494931215No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT18741.2750207514049723No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT18691.271618881737403No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT18311.2457646722638762No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT17831.2131067234552109No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT17301.1770469049789765No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT16621.130781477500034No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC16261.106288015893535No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC16091.0947216590237996No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT15881.0804338064200085No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT15381.0464151097443153No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT15271.038930996475663No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT15191.0334880050075521No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT15101.0273646396059275No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT14951.0171590306032197No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC14751.0035515519329423No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC14670.9981085604648314No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC14670.9981085604648314No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC14550.9899440732626651No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT14460.9838207078610405No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC14370.9776973424594156No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12440.846385173291241No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGGCGCAAGCCTGAC12410.8443440514906992No Hit
CCTACGGGTGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC12410.8443440514906992No Hit
CCTACGGGGGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC11770.8008001197458123No Hit
CCTACGGGAGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC11280.7674617970036332No Hit
CCTACGGGAGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC11060.7524935704663283No Hit
CCTACGGGCGGCTGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10800.7348038481949679No Hit
CCTACGGGGGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10800.7348038481949679No Hit
CCTACGGGTGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC10550.7177944998571215No Hit
CCTACGGGCGGCAGCAGTCGGGAATATTGCGCAATGGAGGAAACTCTGAC9860.6708486984446652No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC5870.3993794989726354No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC5650.38441127243533046No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC5510.3748860373661364No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC5020.34154771462395733No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC4980.3388262188899019No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC4980.3388262188899019No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC4830.328620609887194No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGACGCAAGTCTGAC4710.32045612268502766No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4710.32045612268502766No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4640.3156935051504307No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC4310.29324116534447336No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC4220.2871177999428486No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC4020.2735103212725714No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3930.2673869558709467No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC3910.26602620800391896No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3880.2639850862033774No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3860.2626243383363497No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC3810.25922246866878035No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3770.25650097293472496No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC3630.2469757378655309No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC3480.236770128862823No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC3370.22928601559417056No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC3340.22724489379362897No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3050.20751404972172705No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGCAACTCTGAC3050.20751404972172705No Hit
CCTACGGGTGGCTGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA3040.2068336757882132No Hit
CCTACGGGGGGCTGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA2910.19798881465253304No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2880.19594769285199146No Hit
CCTACGGGAGGCAGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA2840.19322619711793604No Hit
CCTACGGGCGGCTGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA2760.18778320564982515No Hit
CCTACGGGGGGCAGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA2660.18097946631468656No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2650.1802990923811727No Hit
CCTACGGGTGGCAGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA2650.1802990923811727No Hit
CCTACGGGAGGCTGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA2650.1802990923811727No Hit
CCTACGGGCGGCAGCAGTTAGGAATATTCGTCAATGGAGGAAACTCTGAA2580.17553647484657567No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2540.17281497911252025No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2500.1700934833784648No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2490.16941310944495094No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2490.16941310944495094No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2390.16260937010981236No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2350.1598878743757569No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2290.15580563077467374No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2170.14764114357250746No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2130.14491964783845201No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT2080.1415177781708827No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC2080.1415177781708827No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC1980.13471403883574412No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC1870.12722992556709167No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1820.12382805589952238No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1820.12382805589952238No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1800.12246730803249467No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1800.12246730803249467No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1740.1183850644314115No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGGCAATGGAGGGAACTCTGAC1700.11566356869735607No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGGAACCCTGAT1670.11362244689681449No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1550.10545795969464818No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1510.10273646396059274No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGAGGAAACTCTGAT1490.10137571609356504No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGAGGAAACTCTGAT1470.1000149682265373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)